Scribd
Upload
312 views

FULL LAB 1 BIO615 - Lab Report About DNA Extraction. This Report Contain The Method, Result and - Studocu

The document provides instructions for extracting recombinant proteins from E. coli cell pellets. It involves 9 steps: 1) centrifuging the cell pellet and discarding the supernatant, 2) adding lysis buffer and proteinase K to lyse the cells, 3) incubating at 55°C, 4) adding RNase A and incubating at room temperature, 5) adding binding buffer, 6) transferring to a spin column and centrifuging, 7) centrifuging after adding wash buffer, 8) repeating step 7 with a wash buffer, 9) repeating step 8 twice to wash the cell culture.

Uploaded by

MØ'gireh Bryan
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
312 views

FULL LAB 1 BIO615 - Lab Report About DNA Extraction. This Report Contain The Method, Result and - Studocu

The document provides instructions for extracting recombinant proteins from E. coli cell pellets. It involves 9 steps: 1) centrifuging the cell pellet and discarding the supernatant, 2) adding lysis buffer and proteinase K to lyse the cells, 3) incubating at 55°C, 4) adding RNase A and incubating at room temperature, 5) adding binding buffer, 6) transferring to a spin column and centrifuging, 7) centrifuging after adding wash buffer, 8) repeating step 7 with a wash buffer, 9) repeating step 8 twice to wash the cell culture.

Uploaded by

MØ'gireh Bryan
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
You are on page 1/ 1

Search for courses, books or documents Ask AI Sign in

Download 5 1 Save Share

tube was centrifuged at 12,000xg for 1 minute and then the supernatant was discarded
BIO615 LAB Report 2 - Feel free to use it …
completely. Was this document helpful?
1. LAB Manual BIO 615 latest edition (2019 )
2. 100 µl Lysis buffer (LB11) and 20 µl Proteinase K were added into the tube. The E.
Extraction of recombinant proteins from … 5 1
Coli cell pellet was resuspended by vortexing or pipetting.
Experiment 1 biomol
3. Then, the mixture was incubated at 55Oc for 15 minutes in the water bath.
EUA Xiamen SARSkit ifu - Helpful lab rep… 4. 20 µl RNase A was added to the sample and after that the mixture was incubated at
room temperature for 2 minutes.
Related Studylists
5. 50 µl Binding buffer (BB11) was added and vortex machine was used to mix the
my studylist
solution for 30 seconds.
TBM
6. Next, the entire contents from the microcentrifuge tube were transferred to a spin
uni
column and it was centrifuged at 12,000xg form 30 seconds. The flow through of the spin
column were discarded completely.
Preview text
Electrophoresis. The Wolbachia Project.
7. Centrifuge the mixture again at 12,000xg for 30 seconds after adding 500
Stuckµl on
clean
your studies?
Retrieved from cdn.vanderbilt/vu-wp0/wp- buffer (CB211). The flow through of the spin column were discarded completely. Get instant help powered by AI
content/uploads/sites/267/2021/06/02163125/
8. The step number 7 was repeated but using different buffer which is wash buffer
212 Ask AI
(WB11). The flow through were discarded completely after the mixture were centrifuged.
9. The step 8 was recommended to be done twice to ensure the cell culture was washed

You might also like