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LAB 12 Blood

This document provides procedures for analyzing a blood sample to determine its key constituents. It outlines tests to identify chlorides, phosphates, glucose, and iron. The objectives are to differentiate between oxyhemoglobin and reduced hemoglobin, and determine the different constituents of blood. A series of chemical tests are performed on blood and its filtrate to achieve these objectives, including the hemin test, benzidine test, and Benedict's test.

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Mike Bryan Blanco
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265 views

LAB 12 Blood

This document provides procedures for analyzing a blood sample to determine its key constituents. It outlines tests to identify chlorides, phosphates, glucose, and iron. The objectives are to differentiate between oxyhemoglobin and reduced hemoglobin, and determine the different constituents of blood. A series of chemical tests are performed on blood and its filtrate to achieve these objectives, including the hemin test, benzidine test, and Benedict's test.

Uploaded by

Mike Bryan Blanco
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as DOCX, PDF, TXT or read online on Scribd
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CENTER FOR NATURAL SCIENCES

Name: Course & Year: Score:


Instructor: Group No: Date of Activity:

Activity 12
Analysis of Blood

Theory: Blood is a type of connective tissue with a liquid matrix and is the most
active transport system of the body. Blood plasma is the most physiologically active tissue
of the body. The proteins of the plasma are diverse and complex, serving multiple
functions. The most important are albumin, globulin and fibrinogen

OBJECTIVES
1. To determine the different constituents of the blood
2. To differentiate oxyhemoglobin from reduced hemoglobin

MATERIALS

Blood sample 5% AgNO3 Flask


10% HCl 3% H2O2 Beaker
10% NH4OH Benedict’s solution Funnel evaporating dish
10% HAc 1% NaCl Test tubes
10% N HCl 0.05% CuSO4 solution Graduated cylinder
5%potassium Glacial acetic acid microscope
thiocyanate Stokes reagent aspirator
1% benzidine Filter paper pipette
5% NH4MoO4 Litmus paper

PROCEDURES

A. General Test for Blood

Place 10 mL of freshly drawn blood in a heparinized container. Shake and subject to


the following tests.

1. Hemin Test
Place 1 drop of heparinized blood on a glass slide then add 1 drop of 1 % NaCl in a
glacial acetic acid. Evaporate the mixture over a water bath. Cool and examine under
the microscope. Draw what you have seen and label

2. Benzidine Test
Prepare dilute blood by mixing 1 drop of blood with 5 mL of distilled water. Into 2
mL of the diluted blood, add 6 mL of 1 % benzidine solution and 2 mL of 3 % H 2O2
solution. Observe the formation of green or blue color. Describe the result below.
What is the significance of the formation of blue color? Write the reaction involved.

B. Test for Blood Constituents


CENTER FOR NATURAL SCIENCES

Place 2.5 mL of freshly-drawn blood or a defibrinated blood with 25 mL of


distilled water in a small beaker. Heat to boiling then add 4 drops of 10% acetic
acid and continue heating for few more minutes. Filter the solution while hot. Set
aside the residue for the test for iron and use the filtrate for the following tests.

1. Test for Chlorides


Acidify 1 mL of filtrate using 0.1 M HNO3, then add 2 to 3 drops 5% AgNO3
solution. A white precipitate indicates the presence of chlorides.

2. Test for the Presence of Phosphates


Acidify 2 mL of the filtrate with 0.1M HNO 3 then add 4 mL of 5% Ammonium
molybdate solution. The formation of yellow precipitate means a positive result.

3. Benedict’s Test
Place 2 mL of the filtrate in a test tube, then add 4 drops of Benedict’s reagent.
Heat the mixture over a water bath and observe the formation of brick red
precipitate.

4. Test for Iron


Heat the residue that was set aside in an evaporating dish until all the organic
matter has completely burned out. Cool then add 2 to 3 mL of 10% HCl solution.
To the dissolved material add 3 to 4 drops of 5% KSCN solution and take note of
the color produced.

RESULTS AND DISCUSSION

Tests Description
Chlorides
Phosphates
Benedict’s
Iron

GENERALIZATION:

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