Diffusion-

• Diffusion is the movement of molecules from a region of its higher concentration to

a region of its lower concentration
• Molecules move down a concentration gradient, as a result of their random

movement
Diffusion across the cell membrane
• For living cells, the principle of the movement down a concentration gradient is the
same, but the cell is surrounded by a cell membrane which can restrict the free
movement of the molecules
• The cell membrane is a partially permeable membrane - this means it allows some
molecules to cross easily, but others with difficulty or not at all
• The simplest sort of selection is based on the size of the molecules
• Diffusion helps living organisms to:
o obtain many of their requirements
o get rid of many of their waste products
o carry out gas exchange for respiration
Examples of diffusion in living organisms
• You will need to learn examples of substances that organisms obtain by diffusion
• Don’t forget that plants require oxygen for respiration at all times, as well
as carbon dioxide for photosynthesis when conditions for photosynthesis are right
(e.g. enough light and a suitable temperature)
Examples of Diffusion Table
Where does the energy for diffusion come from?
• All particles move randomly at all times
• This is known as Brownian motion
• The energy for diffusion comes from the kinetic energy of this random movement of
molecules and ions

Brownian motion
 Factors that Influence Diffusion
Surface area to volume ratio

• The bigger a cell or structure is, the smaller its surface area to volume ratio is,
slowing down the rate at which substances can move across its surface
• Many cells which are adapted for diffusion have increased surface area in some way
- eg root hair cells in plants (which absorb water and mineral ions) and cells lining the
ileum in animals (which absorb the products of digestion)

Cell adaptations for diffusion

The highly folded surface of the small intestine increases its surface area
Distance
 • The smaller the distance molecules have to travel the faster transport will occur
• This is why blood capillaries and alveoli have walls which are only one cell thick,
ensure the rate of diffusion across them is as fast as possible

Temperature

• The higher the temperature, the faster molecules move as they have more energy
• This results in more collisions against the cell membrane and therefore a faster rate of
movement across them

Concentration Gradient

• The greater the difference in concentration on either side of the membrane, the faster
movement across it will occur
• This is because on the side with the higher concentration, more random collisions
against the membrane will occur
 Water as a Solvent
• Water is important for all living organisms as many substances are able to dissolve
in it (it is a solvent)
• This makes it incredibly useful and essential for all life on Earth
• Water is important as a solvent in the following situations within organisms:
o Dissolved substances can be easily transported around organisms - eg xylem
and phloem of plants and dissolved food molecules in the blood
o Digested food molecules are in the alimentary canal but need to be moved to
cells all over the body - without water as a solvent this would not be able to
happen
o Toxic substances such as urea and substances in excess of requirements such
as salts can dissolve in water which makes them easy to remove from the
body in urine
o Water is also an important part of the cytoplasm and plays a role in
ensuring metabolic reactions can happen as necessary in cells

Water as a solvent
 Osmosis
• All cells are surrounded by a cell membrane which is partially permeable
• Water can move in and out of cells by osmosis
• Osmosis is the diffusion of water molecules from a dilute solution (high
concentration of water) to a more concentrated solution (low concentration of
water) across a partially permeable membrane
• In doing this, water is moving down its concentration gradient
• The cell membrane is partially permeable which means it allows small
molecules (like water) through but not larger molecules (like solute molecules)

Osmosis and the partially permeable membrane

Osmosis Experiments
Immersing plant cells in solutions of different concentrations
• The most common osmosis practical involves cutting cylinders of root
vegetables such as potato or radish and placing them into distilled water and sucrose
solutions of increasing concentration
• The cylinders are weighed before placing into the solutions
• They are left in the solutions for 20 - 30 minutes and then removed, dried to remove
excess liquid and reweighed
Potatoes are usually used in osmosis experiments to show how the concentration of a
solution affects the movement of water, but radishes can be used too
• If the plant tissue gains mass:
o Water must have moved into the plant tissue from the solution surrounding it
by osmosis
o The solution surrounding the tissue is more dilute than the plant tissue (which
is more concentrated)
• If plant tissue loses mass:
o Water must have moved out of the plant tissue into the solution surrounding it
by osmosis
o The solution surrounding the tissue is more concentrated than the plant tissue
(which is more dilute)
• If there is no overall change in mass:
 o There has been no net movement of water as the concentration in both the
plant tissue and the solution surrounding it must be equal
o Remember that water will still be moving into and out of the plant tissue, but
there wouldn’t be any net movement in this case
Investigating osmosis using dialysis tubing
• Dialysis tubing (sometimes referred to as visking tubing) is a non-living partially
permeable membrane made from cellulose
• Pores in this membrane are small enough to prevent the passage of large
molecules (such as sucrose) but allow smaller molecules (such as glucose and
water) to pass through by diffusion and osmosis
• This can be demonstrated by:
o Filling a section of dialysis tubing with concentrated sucrose solution
o Suspending the tubing in a boiling tube of water for a set period of time
o Noting whether the water level outside the tubing decreases as water moves
into the tubing via osmosis
▪ Water moves from a region of higher water potential (dilute solution)
to a region of lower water potential (concentrated solution), through a
partially permeable membrane

An example setup of a dialysis tubing experiment

 Osmosis in Plant Tissues
• When water moves into a plant cell, the vacuole gets bigger, pushing the cell
membrane against the cell wall
• Water entering the cell by osmosis makes the cell rigid and firm
• This is important for plants as the effect of all the cells in a plant being firm is
to provide support and strength for the plant - making the plant stand upright with
its leaves held out to catch sunlight
• The pressure created by the cell wall stops too much water entering and prevents the
cell from bursting
• If plants do not receive enough water the cells cannot remain rigid and firm (turgid)
and the plant wilts

Osmosis: Extended
• Osmosis is the net movement of water molecules from a region of higher water
potential (dilute solution) to a region of lower water potential (concentrated
solution), through a partially permeable membrane
• It can get a little confusing to talk about the 'concentration of water' when we also talk
about solutions being ‘concentrated’ (having a lot of solute in them), so instead we
can say that a dilute solution has a high water potential (the right-hand side of the
diagram below) and a concentrated solution has a low water potential (the left-hand
side of the diagram below)
How osmosis works
 Osmosis in Animals & Plants: Extended
Plant cells in solutions of different concentrations
• When plant cells are placed in a solution that has a higher water potential (dilute
solution) than inside the cells (e.g. distilled water) then water moves into the plant
cells via osmosis
• These water molecules push the cell membrane against the cell wall, increasing
the turgor pressure in the cells which makes them turgid

A turgid plant cell

• When plant cells are placed in a concentrated solution (with a lower water potential
than inside the cells) water molecules will move out of the plant cells by osmosis,
making them flaccid
o If plant cells become flaccid it can negatively affect the plant's ability to
support itself
• If looked at underneath the microscope, the plant cells might be plasmolysed,
meaning the cell membrane has pulled away from the cell wall
 A plasmolysed plant cell
Animal cells in solutions of different concentrations
• Animal cells also lose and gain water as a result of osmosis
• As animal cells do not have a supporting cell wall, the results on the cell are more
severe
• If an animal cell is placed into a strong sugar solution (with a lower water potential
than the cell), it will lose water by osmosis and become crenated (shrivelled up)
• If an animal cell is placed into distilled water (with a higher water potential than the
cell), it will gain water by osmosis and, as it has no cell wall to create turgor
pressure, will continue to do so until the cell membrane is stretched too far and it
bursts
Effect of osmosis on animal cells
 Active Transport
• Active transport is the movement of particles through a cell membrane from a region
of lower concentration to a region of higher concentration using energy from
respiration

The process of active transport

 Importance of Active Transport: Extended
• Energy is needed because particles are being moved against a concentration
gradient, in the opposite direction from which they would naturally move (by
diffusion)
• Active transport is vital process for the movement of molecules or ions across
membranes
• Including:

o uptake of glucose by epithelial cells in the villi of the small intestine and by
kidney tubules in the nephron
o uptake of ions from soil water by root hair cells in plants

Protein Carriers: Extended

• Active transport works by using carrier proteins embedded in the cell membrane to
pick up specific molecules and take them through the cell membrane against their
concentration gradient:

1.
1. Substance combines with carrier protein molecule in the cell membrane
2. Carrier transports substances across membrane using energy from respiration
to give them the kinetic energy needed to change shape and move the
substance through the cell membrane
3. Substance released into cell

Carrier proteins in active transport