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Class 9 Experiment 4 Onion Cell, Cheek Cell

This document provides instructions for two experiments: 1) Preparing a stained temporary mount of onion peel cells to observe their plant-like characteristics under a microscope. Onion peel cells have rectangular shapes, distinct cell walls, cytoplasm, large central vacuoles, and nuclei. 2) Preparing a stained temporary mount of human cheek cells to observe their animal-like characteristics. Cheek cells are flat, irregularly shaped, lack cell walls and vacuoles, and have jelly-like cytoplasm enclosing the central nuclei. Both experiments aim to distinguish key features of plant and animal cells under the microscope.

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ARSHLEEN KAUR GHAI
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7K views

Class 9 Experiment 4 Onion Cell, Cheek Cell

This document provides instructions for two experiments: 1) Preparing a stained temporary mount of onion peel cells to observe their plant-like characteristics under a microscope. Onion peel cells have rectangular shapes, distinct cell walls, cytoplasm, large central vacuoles, and nuclei. 2) Preparing a stained temporary mount of human cheek cells to observe their animal-like characteristics. Cheek cells are flat, irregularly shaped, lack cell walls and vacuoles, and have jelly-like cytoplasm enclosing the central nuclei. Both experiments aim to distinguish key features of plant and animal cells under the microscope.

Uploaded by

ARSHLEEN KAUR GHAI
Copyright
© © All Rights Reserved
Available Formats
Download as PDF, TXT or read online on Scribd
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CBSE Class 9 Science Practical Skills – Slide of Onion Peel and Cheek Cells

EXPERIMENT 4(a)

AIM
To prepare stained temporary mount of onion peel cells and to record observations and
draw labelled diagrams.

MATERIALS REQUIRED
Onion, plain slides, coverslip, watch glass, needles, forceps, brush, blade, safranin, blotting
paper, glycerine and compound microscope.

THEORY
Onion is a multicellular plant. Like other plant cells, the cell of onion peel consists of a cell
wall, cell membrane, cytoplasm, a large vacuole and a nucleus. The nucleus lies at the
periphery of cytoplasm and vacuole is located in the centre. Presence of large vacuoles and
cell wall confirms that cells of onion peel are plant cells.

PROCEDURE

1. Take a piece of onion and bend it to remove the transparent membranous structure
called onion epidermal peel. With help of forcep remove the peel from its inner side.
2. Place the peel in water in a watch glass.
3. Add a few drops of stain safranin, to the watch glass containing the peel for staining.
4. Now, wash the leaf peel with water and transfer it on to a clean slide with the help
of brush.
5. Remove extra water from the slide surrounding the peel with the help of blotting
paper.
6. To this slide, add a drop of glycerine over the peel and place the coverslip in a
manner to avoid entry of air bubbles.
7. Soak away the extra glycerine with blotting paper.
8. Examine slide under the microscope.

OBSERVATIONS

1. A large number of rectangular cells with distinct cell wall can be observed.
2. Cytoplasm is seen as thin layer of deep coloured substance on inner surface of cell
wall.
3. A big central vacuole is present in the cell.
4. A deeply stained round body called nucleus is seen in each cell.
RESULT

1. The epidermal peel of onion comprises of rectangular shaped cells. Each cell
comprises of a nucleus, a central vacuole, thin layer of cytoplasm and cell wall.
2. As cell walls and large prominent vacuole are present in each cell, the cells placed
under observation are plant cells.

PRECAUTIONS

1. Always take a clean slide and hold it by its edges to avoid making the slide dirty.
2. Peel should be properly stain. Avoid under-staining or excessive staining of the peel.
3. Always transfer the peel with the help of brush.
4. Mounting of the peel should be done in centre of slide.
5. Avoid folding of the leaf peel.
6. Remove extra glycerine with the help of blotting paper.
7. Avoid entry of air bubbles while placing the cover slip.

EXPERIMENT 4(b)

AIM To prepare stained temporary mount of human cheek cells and to record observations
and draw labelled diagrams.

MATERIALS REQUIRED Slide, cover slip, needle, blotting paper, tooth pick, methylene blue,
watch glass, compound microscope

THEORY
Animal cells lack cell wall and prominent vacuole. In these cells a semi-permeable
membrane called cell membrane surrounds the cytoplasm. In comparison to plant cell, in
animal cell cytoplasm is dense and occupies larger space. Vacuoles are absent or very small
in size. In animal cells, the position of nucleus is usually central.Cheek cells are part of
squamous epithelium. They are thin and flat cells. They found arranged like pavement tiles,
i.e., arranged end to end forming a delicate lining.

PROCEDURE

1. Rinse your mouth to remove any food particles.


2. With the help of the toothpick scrap the inner side of the cheek to get some
epithelial cells.
3. Place the scrappings in a drop of water over a clean slide.
4. Spread the scrappings with the help of needle.
5. Add a drop of methylene blue over the slide.
6. Wait for 2 minutes and then remove the extra stain with blotting paper.
7. Add a drop of glycerine on the slide.
8. With the help of needle, gently place the coverslip over the slide to avoid entry of air
bubbles.
9. Observe the slide under the microscope.

OBSERVATIONS

1. Large number of flat polygonal cells with irregular boundaries attached edge to edge
are seen.
2. The cells have thin membrane called cell membrane which encloses jelly like
substance called

RESULT
Cells under observation do not have cell wall and large prominent vacuole. So, cells of
specimen on the slide are animal cells.

PRECAUTIONS

1. Scrap the cheek cells gently with help of clean toothpick to avoid injury.
2. Always rinse the mouth before scrapping the tissue so as to remove any food
particles from mouth.
3. Place coverslip gently to avoid entry of air bubbles.
4. Remove extra glycerine with blotting paper.
5. Spread the scrappings so that cells are properly distributed.

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