One of The World’s Leading HPLC Columns

Explore Successful Separations/Phase Overview.....................................................................4

Explore Robust Methods.........................................................................................................5
Explore Options for Every Development Route.........................................................................6
Luna C18(2), C8(2), C5............................................................................................................8
Luna PFP(2)...........................................................................................................................16
Luna Phenyl-Hexyl.................................................................................................................18
Luna CN (Cyano)...................................................................................................................20
Luna NH2 (Amino)..................................................................................................................21
Luna HILIC............................................................................................................................22
Luna SCX..............................................................................................................................24
Axia-Packed Preparative HPLC.............................................................................................25
Fast LC.................................................................................................................................26
Ordering Information..............................................................................................................28
One of the world’s leading HPLC columns
The Luna® brand of columns and media is more than just a product line from Phenomenex. It is a
pledge to provide you with the highest level of satisfaction for your chromatographic goals. Every
aspect of Luna products has been engineered to meet the exacting demands placed on today’s
chromatographers.

Luna products continue to uphold the quality our customers depend on. If you have never
tried Luna columns or media, this brochure will guide you through the various solutions
to fit your needs.

For those who use Luna products daily, thank you for making Luna columns one of the world’s
leading HPLC columns.

© 2008 Phenomenex, Inc. All rights reserved.

Explore Successful Separations
Your success begins with our commitment to provide the essential solutions to HPLC separations in the Luna
brand. Some of the highest quality and performance standards are incorporated into Luna products, making them
an indispensable platform for all areas of HPLC.

Explore Resolution with Luna Selectivities

Phase selectivity has the strongest impact on overall chromatographic resolution. Choosing the optimal selectivity
can drive your separation to success. Luna phases span through 10 different chemistries, each offering its own
unique selectivity.

Luna Phases Description Particle Size (µm) Pore Surface Carbon Bonded Phase pH Stability Application Reversed Normal HILIC IEX
Size (Å) Area (m2/g) Load (%) Coverage (µmole/m2) Phase Phase
Silica(2) Unbonded silica 3, 5, 10, 10-PREP, 15 100 400 — — 2.0 - 7.5 Non-polar compounds 

C5 5 Carbon ligand 5, 10 100 440 12.5 7.85 1.5 - 9.0* Good alternative to C8 when less retention 
is desired
C8(2) C8 ligand optimized for improved peak shape 3, 5, 10, 10-PREP, 15 100 400 13.5 5.50 1.5 - 9.0* Great starting phase for method development 

C18(2) C18 ligand optimized for improved peak shape 2.5, 3, 5, 10, 100 400 17.5 3.00 1.5 - 9.0* From capillary LC/MS to process scale 
OUR MOST POPULAR PHASE
10-PREP, 15
CN Versatile CN phase 3, 5, 10 100 400 7.0 3.80 1.5 - 7.0 For improving the retention of polar  
compounds
NH2 Rugged and reproducible NH2 3, 5, 10 100 400 9.5 5.80 1.5 - 11 Sugar alcohols, anionic or hydrogen bonding   
compounds
Phenyl-Hexyl Phenyl phase attached to C6 (hexyl) ligand 3, 5, 10, 10-PREP, 15 100 400 17.5 4.00 1.5 - 9.0* Unique selectivity for very polar and aromatic 
compounds
SCX Benzene sulfonic acid 5, 10 100 400 Binding Capacity: 0.15 meq/g 2.0 - 7.0 Amine and polyamine containing compounds 

HILIC Reproducible, cross-linked diol 3, 5 200 200 5.7 4.30 1.5 - 8.0 Increased retention and MS sensitivity of 
polar compounds
PFP(2) Pentafluorophenyl with a C3 (propyl) linkage 3, 5 100 400 11.5 2.2 1.5 - 8.0 Highly polar compounds, halogenated 
compounds and isomers

* pH range is 1.5 - 10 under isocratic conditions. pH range is 1.5 - 9 under gradient conditions.

4 Phenomenex l WEB: www.phenomenex.com

 Explore Robust Methods
Successful methods depend on results that can tolerate minor variations in chromatographic parameters. The base
silica of Luna is 99.999 % pure and meticulous care is given to quality control over all aspects of silica structure
and chemistry. This ensures that Luna columns will always perform consistently, resulting in method reproducibility
you can trust.

Reliable Performance Column to Column Reproducibility

The chromatograms in Figure 2 show consistency of inertness
Almost no variation is observed among the batches of Luna. Figure 1 shows quality control test data designed
(black) and hydrophobicity (blue) for Luna 5 µm C18(2) columns from
to monitor the slightest differences that may affect reproducibility - particle shape and smoothness, porosimetry,
9 different batches. Almost no variation is observed.
bonding consistency and pH stability.

Particle Size

Surface Coverage
Particle Size Distribution

Batch No.

Carbon Load % Pore Size (x100 Å) 7

Figure 1 9
Silica Reproducibility.
Batch No: Inert Test
Hydrophobicity

Figure 2
Column-to-column
reproducibility for 9 batches of Luna
5 µm C18(2)

Metal (ppm/10) Surface Area (x100 m2/g)

Phenomenex l WEB: www.phenomenex.com 5

 Explore Options for Every Development Route
Luna® media is available in a wide variety of particle sizes and formats, each with different attributes that can be
optimized for nearly any stage of development.

Direct Connect
Cartridge Holder

Fast LC-MS Methods High-Speed-Technology

Standard Luna media is available in MercuryMS™ Cartridges Luna 2.5 µm C18(2)-HST columns deliver
Cartridge Holder
20 x 2.0 mm
and online columns for quick, cost-effective highly efficient separations without the need for
Cartridge screening methods. expensive high-pressure instruments.

Develop Robust Analytical Methods Lab-Scale Purification Redefined

Analytical HPLC columns are the most widely used format Axia™-packed Luna preparative columns provide
and are available in a wide variety of dimensions and industry-leading lifetimes and efficiencies.
particle sizes.

6 Phenomenex l WEB: www.phenomenex.com

Beyond our largest preparative column dimensions, Luna phases are
available in bulk quantities for HPLC purification at the process, pilot, and
commercial scale. The highly reproducible manufacturing process makes
scaling to large scale purification extremely straight-forward.

The wide range of Luna phases provides you with the selectivity choices
to optimize parameters such as retention time and resolution. Additionally,
the high surface area (400 m2/g) of Luna materials gives you greater
loadability than most other media. For those challenging purifications
where chromatography is the best option, the Luna family offers an
excellent platform for all purification challenges.


Contact your Phenomenex technical
consultant for bulk media sales.

Phenomenex l WEB: www.phenomenex.com 7

Luna C18(2), C8(2), C5
Your Starting-Point for All Reversed Phase Methods Pyridine Peak Asymmetry Comparison

Luna has found a place as one of the world’s top reversed phase columns because it can help Luna® 5 μm C18 (2)
optimize two important chromatographic properties: resolution and peak shape. The high efficiencies
and bonded phase surface coverage provide for sharp peaks. Whether you need a column for USP Sigma-Aldrich Biotechnology
Supelcosil™ 5 μm ABZ
methods or just general method development, Luna C18(2) and C8(2) should be your first choice
every time. Sigma-Aldrich Biotechnology
Supelcosil™ LC-18-BD

The result: Waters

Spherisorb® 5 μm ODS (2)
 Free exposed silanols virtually eliminated by complete bonding and endcapping Agilent Technologies
T
 Sharp peak shape for good method sensitivity ZORBAX® 5 μm SB-C18
G.L. Sciences
 pH stable from 1.5 to 10.0 for over 10,000 hours
Inertsil® ODS (2)
Waters
Symmetry® 5 μm C18
0 1 2 3 4

Peak Symmetry(A ) S

Comparison of 7 different 5 μm reversed phase columns. This survey measures the degree of silanol activity on the surface of
each silica. In this survey, Luna 5 µm C18(2) material demonstrates the lowest silanol activity.

PEAK ASYMMETRY COMPARISON OF COMPETING COLUMNS

Conditions for all columns

Phenomenex Luna 5 μm C18(2) ® Dimension: 150 x 4.6 mm

Mobile Phase: Acetonitrile/Water (50:50)
Flow Rate: 1.0 mL/min
Detection: UV @ 254 nm
Waters Agilent Technologies Sample: 1. Pyridine
Pyridine Asymmetry
Symmetry 5 μm C18 ZORBAX 5 μm SB-C18 2. Phenol
3. Toluene
2
As =1.44 2
500
Pyridine Adsorption:
300 No elution
1 400
400 1 Pyridine Asymmetry: 3
2 A s 2.27 300
200 3
300
App ID 1100

200
200 3
100
100 100 1

0 0 0
0 2 4 6 8 10 12 min 0 2 4 6 8 10 12 min 0 2 4 6 8 10 12 min

Agilent Technologies and ZORBAX are registered trademarks of Agilent Technologies, Inc. Inertsil is a registered trademark of GL Sciences. Supelcosil is a trademark of Sigma-Aldrich Biotechnology. Waters and Symmetry are registered trademarks of Waters Corporation.
Phenomenex is not affiliated with Agilent Technologies, GL Sciences, Sigma Aldrich, or Waters Corporation. The comparative data presented here may not be representative for all applications.

8 Phenomenex l WEB: www.phenomenex.com

Applications
Polar, Acidic Drugs Conditions same for both columns Hydrophobic, Acidic Compounds Conditions same for both columns
Dimension: 75 x 4.6 mm Dimension: 150 x 4.6 mm
Mobile Phase: 20 mM KH2PO4/ Acetonitrile(70:30) Mobile Phase: 20 mM Potassium phosphate, pH 2.5 /
Phenomenex Luna 5 µm C18(2) Acetonitrile (75:25)
Phenomenex Luna® 3 µm C18(2) Flow Rate: 0.75 mL/min
Flow Rate: 1.5 mL/min
Waters® Symmetry® 3.5 µm C18 Detection: UV @ 202 nm Thermo Hypersil-Keystone® HyPURITY™ Elite 5 µm C18
Sample: 1. Tolmetin Temperature: 30 °C
2. Naproxen Detection: UV @ 254 nm
3. Diflunisal Sample: 1. p-Hydroxybenzoic acid
4. Fenoprofen 2. Sorbic acid*
5. Indomethacin 3. Benzoic acid*
6. Ibuprofen 4. Salicylic acid
5. p-Toluic acid
* Sorbic acid and Benzoic
acid co-elute on HyPURITY Elite

App ID 14314 App ID 14315

App ID 12904
App ID 12903
Basic Compounds
Conditions same for both columns
α- and β-acids in Hop Extract
Column: Luna 5 µm C18(2)
Dimension: 150 x 4.6 mm Dimension: 250 x 4.6 mm
Phenomenex Luna 5 µm C18(2) Mobile Phase: 20 mM Potassium phosphate, pH 2.5 / Part No.: 00G-4252-E0
Acetonitrile (75:25) Mobile Phase: Methanol with 0.1 % H3P04
Macherey-Nagel® Nucleosil® 5 µm C18
Flow Rate: 1.5 mL/min / Water with 0.1 % H3P04 (90:10)
Temperature: 30 °C Flow Rate: 1.5 mL/min
Detection: UV @ 210 nm Temperature: 30 °C
Sample: 1. Maleic acid Detection: UV @ 314 nm
2. Triprolidine* Sample: 1. Cohumulone
3. Chlorpheniramine* 2. Ad-+humulone
4. Diphenhydramine* 3. Colupulone
*Peaks 2-4 adsorb on Nucleosil C18 4. Ad-+lupulone
App ID 14317

App ID 14313
App ID 14316

HyPURITY is a trademark of Thermo Hypersil-Keystone. Waters and Symmetry are registered trademarks of Waters Corporation. Nucleosil is a registered trademark of Macherey-Nagel. Phenomenex is not affiliated with
any of the above companies. Comparative separations may not be representative of all applications.

Phenomenex l WEB: www.phenomenex.com 9

 Applications
USP METHOD: ESTRADIOL COCAINE-NARCOTIC DRUG

Column: Luna® 5 µm C18(2) Column: Luna 5 µm C18(2)

Dimension: 150 x 4.6 mm Dimension: 150 x 4.6 mm
Part No.: 00F-4252-E0 Part No.: 00F-4252-E0
1 Mobile Phase: Phosphate Buffer, pH 2.5/
Mobile Phase: Acetontrile/Water (55:45)
Acetonitrile (75:25)
Flow Rate: 1 mL/min
Flow Rate: 1 mL/min
Temperature: 30 °C
Temperature: 30 °C
Detection: UV @ 254 nm
Detection: UV @ 233 nm
Sample: 1. Ethylparaben 1
2. Estrone mAU Sample: 1. Cocaine hydrochloride
3. Estradiol 600

2 500

400

App ID 10893

App ID 1313
300

200
3
100

0
1 2 3 4 min
0 2 4 6 min

USP METHOD: PHENYLEPHRINE HYDROCHLORIDE INJECTION USP METHOD: HYDROCORTISONE CREAM

1
Column: Luna 5 µm C18(2) Column: Luna 5 µm C18(2)
Dimension: 250 x 4.6 mm Dimension: 250 x 4.6 mm
Part No.: 00G-4252-E0 Part No.: 00G-4252-E0
Mobile Phase: Water/Methanol with 1.1 % Mobile Phase: Water/Acetonitrile (75:25)
1-Octanesulfonic Flow Rate: 2 mL/min
acid pH 3.0 (50:50)
Temperature: 30 °C
Flow Rate: 1 mL/min
Detection: UV @ 254 nm
Temperature: 22 °C
2
Sample: 1. Hydrocortisone
Detection: UV @ 280 nm 1
Sample: 1. Phenylephrine
hydrochloride
2. Epinephrine bitartrate
App ID 3246

App ID 3271
0 2 4 6 8 min

10 Phenomenex l WEB: www.phenomenex.com

 Applications
EPA METHOD 8330 - EXPLOSIVES EPA METHOD 8310 - POLYNUCLEAR AROMATIC HYDROCARBONS (PAHs)

4
Column: Luna® 5 µm C18(2) Column: Luna 5 µm C18(2)
Dimension: 250 x 4.6 mm Dimension: 250 x 4.6 mm
Part No.: 00G-4252-E0 Part No.: 00G-4252-E0
3 Mobile Phase: Methanol/Water (55:45) Mobile Phase: A: Water B: Acetonitrile
Flow Rate: 1 mL/min Gradient: A/B (25:75) to 100 % B in 25 min
Temperature: 35 °C Flow Rate: 2 mL/min
Detection: UV @ 254 nm Temperature: 22 °C
5 11
Sample: 1. HMX Detection: UV @ 254 nm
1 2. RDX
7 Sample: 1. Naphthalene
3. 1,3,5-Trinitrobenzene 2. Acenaphthalene
2 6 9
4. 1,3-Dinitrobenzene 3. Fluorene
5. Tetryl 4. Phenanthrene
8 10 6. Nitrobenzene 5. Anthracene
7. 2,4,6-Trinitrotoluene 6. Fluoranthracene

App ID 14263
12 8. 4-Amino-2,6-Dinitriotoluen
14 7. Pyrene

App ID 14269
13 9. 2-Amino-4,6-Dinitrotoluene 8. Benz[a]anthracene
10. 2,6-Dinitrotoluene 9. Chrysene
11. 2,4-Dinitrotoluene 10. Benzo[e]pyrene
12. 2-Nitrotoluene 11. Benzo[b]fluoranthene
13. 4-Nitrotoluene 12. Benzo[k]fluoranthene
14. 3-Nitrotoluene 13. Benzo[a]pyrene
0 2 4 6 8 10 12 14 16
14. Dibenzo[a,h]anthracene
15. Benzo[g,h,i]perylene
16. Indenol[1,2,3-c,d]pyrene

GINGER PUNGENTS COLD MEDICINE

Column: Luna 5 µm C18(2) Column: Luna 5 µm C8(2)

Dimension: 250 x 4.6 mm Dimension: 150 x 4.6 mm
Part No.: 00G-4252-E0 Part No.: 00F-4249-E0
Mobile Phase: A: Water B: Acetonitrile Mobile Phase: Methanol/Acetonitrile
Gradient: A/B (55:45) to A/B (50:50) with 0.1 % H3PO4/ Water
in 8 min, A/B (35:65) in 15 min, with 0.1 % H3PO4 and
A/B (10:90) in 40 min 0.1% Heptane
Flow Rate: 1 mL/min Flow Rate: 1.5 mL/min
Temperature: 50 °C 1 2 Temperature: 22 °C
mAU
Detection: UV @ 282 nm Detection: UV @ 214 nm
m
Sample: 1. 6-Gingerol
175 Sample: 1. Acetaminophen
2. 8-Gingerol + isomer 2. Pseudoephedrine
App ID 12960

150 3
3. 6-Shogaol 3. Benzoic acid

App ID 2578
4. 10-Gingerol 4. Chlorpheniramine
125
5. 8-Shogaol 5. Dextromethorphan
6. 10-Shogaol 100

75

50

25 5
4
0

0 2 4 6 8 min

Phenomenex l WEB: www.phenomenex.com 11

 Applications
WATER SOLUBLE VITAMINS USP METHOD: LORAZEPAM TABLETS

Column: Luna® 5 μm C18(2) Column: Luna 5 μm C18(2)

1
Dimension: 150 x 4.6 mm Dimension: 250 x 4.6 mm
Part No.: 00F-4252-E0 Part No.: 00G-4252-E0
Mobile Phase: 20 mM Potassium Phosphate, Mobile Phase: Water/Methanol/Acetic acid
pH 3.0/Acetonitrile (95:5) (54:44:2)
Flow Rate: 1.5 mL/min Flow Rate: 2 mL/min
Temperature: 22 °C Temperature: 22 °C
Detection: UV @ 214 nm Detection: UV @ 254 nm
Sample: 1. Thiamine Sample: 1. Lorazepam
2. Cyanocobalamin (Vitamin B12)
3. Ascorbic acid
4. Pantothenic acid

App ID 3305
5. Niacinamide

App ID 3253
6. p-Aminobenzoic acid

1.0 2.0 3.0 4.0 5.0 6.0 min

PHARMACEUTICAL PRESERVATIVES USP METHOD: MINOXIDIL

Column: Luna 5 μm C5
Column: Luna 5 μm C18(2)
Dimension: 150 x 4.6 mm
Dimension: 250 x 4.6 mm
mAU 6 8 Part No.: 00F-4043-E0
700 Part No.: 00G-4252-E0
Mobile Phase: A: 0.5 % Acetic acid in water/
Acetonitrile (80:20) Mobile Phase: Methanol/Water/Acetic acid with
1 7 mM Docusate sodium pH 3.0
B: 0.5 % Acetic acid in water/
600 (69.3:29.7:1)
Acetonitrile (20:80) 2
Flow Rate: 1 mL/min
Gradient: A/B (100:0) to A/B (0:100) in 30 min
500 7 Temperature: 22 °C
Flow Rate: 1 mL/min
Detection: UV @ 254 nm
Temperature: 25 °C
Sample: 1. Medroxyprogesterone acetate
400 5 Detection: UV @ 254 nm
2. Minoxidil
Sample: 1. Propylparaben impurity
2. Benzyl alcohol
300 3. Phenol
4. Benzoic acid
5. Methylparaben
200
6. Benzaldehyde
App ID 1141

App ID 3266
7. Ethylparaben
3 8. Propylparaben
100

2 4
1
0

10 20 30 40 min 0 10 20 30 min

12 Phenomenex l WEB: www.phenomenex.com

Applications
USP METHOD: IMIPRAMINE FATTY ACIDS
7

Column: Luna 5 μm C18(2)

® Column: Luna 5 μm C8(2)
Dimension: 250 x 4.6 mm Dimension: 150 x 4.6 mm
Part No.: 00G-4252-E0 Part No.: 00F-4249-E0
Mobile Phase: 0.06 M Sodium perchlorate, pH Mobile Phase: A: Acetonitrile
2.0/Acetonitrile/Triethylamine B: Water (18 Mohms DI)
(62.5:37.5:0.1) Gradient: A/B (70:30)) to A/B (90:10) in 10
8
Flow Rate: 1.5 mL/min 6
min, A/B (90:10) to A/B (70:30) in
Temperature: 22 °C 2 min, hold for 4 min
3
Detection: UV @ 269 nm Flow Rate: 0.3 mL/min
Sample: 1. Imipramine 4 5 Detection: Evaporative Light Scattering
2 (ELSD)
2. Desipramine
1 Temperature: 22 °C
Sample: 1. Lauric acid

App ID 1258
App ID 3255
2. Myristic acid
3. Palmitoleic acid
4. Linoleic acid
5. Palmitic acid
6. Oleic acid
7. Heptadecanoic acid
0 2 4 6 8 10
10 12
12 1
14 min
mi 8. Stearic acid

USP METHOD: NAPROXEN TABLETS USP METHOD: ALBUTEROL TABLETS

Column: Luna 5 μm C18(2) Column: Luna 5 μm C18(2)

Dimension: 150 x 4.6 mm Dimension: 150 x 4.6 mm
Part No.: 00F-4252-E0 Part No.: 00F-4252-E0
Mobile Phase: Acetonitrile/Water/Glacial acid, 1 Mobile Phase: Methanol/Water with 5 mM
pH 3.0 (50:49:1) Hexane sulfonic acid and 1 %
Flow Rate: 1.2 mL/min Glacial acetic acid (40:60)
Temperature: 22 °C Flow Rate: 1.5 mL/min
Detection: UV @ 254 nm Temperature: 22 °C
Sample: 1. Naproxen Detection: UV @ 276 nm
2. Butyrophenone Sample: 1. Albuterol

App ID 3260
App ID 3282

Phenomenex l WEB: www.phenomenex.com 13

 Applications
ANTIOXIDANTS NARCOTICS

Column: Luna® 5 µm C18(2)

18099 Column: Luna 5 µm C18(2)
Dimension: 100 x 4.6 mm
5 Dimension: 150 x 4.6 mm
Part No.: 00D-4252-E0
Part No.: 00F-4252-E0
Mobile Phase: A: Acetonitrile
Mobile Phase: A: 10mM NH4OAc, pH 5.5
B: Phosphate Buffer
B: Acetonitrile
Gradient: A/B (30:70) to A/B (70:30) in 5 min
Gradient: A/B (95:5) for 3 minutes, then A/B
Flow Rate: 1 mL/min (95:5) to A/B (60:40) in 23 minutes
Temperature: 22 °C Flow Rate: 1.0 mL/min
Detection: UV @ 254 nm Temperature: 45 °C
1
Sample: 1. PG 7
2 4 Detection: UV @ 254 nm (ambient)
4 2. TBHQ 2 Sample: 1. Normorphine
3. DMT
2. Morphine
4. BHA

App ID 18099
5 8 3. Hydromorphone

App ID 1283
5. BHT 6
3 4. Norcodeine
1
3 5. Codeine
6. Hydrocodone
7. Cocaine
8. Norcocaine

0.0 1.0 2.0 3.0 4.0 5.0 6.0 7.0 min 0 5 10 15 min

STEROIDS USP METHOD: LORATADINE Column: Luna 3 µm C18(2)

Dimension: 150 x 4.6 mm
Part No.: 00F-4251-E0
Column: Luna 5 µm C8(2) 19565 Mobile Phase: A: 0.96 g 1-pentaesulfonic acid
sodium salt in 1 L buffered to 3.00
Dimension: 150 x 4.6 mm
mAU with Phosphoric Acid
Part No.: 00F-4249-E0 B: Acetonitrile
Mobile Phase: 0.1 % H3PO4 in Water/Acetonitrile/ Gradient: Time (min) Pct B
Methanol (54:35:11) 0 25
Flow Rate: 1.0 mL/min 80 20 50
Temperature: Ambient 30 60
Detection: UV @ 254 nm 35 70
45 70
Sample: 1. Hydrocortisone 60 50 25
2. Corticosterone 1 2
3. 11-α-Hydroxyprogesterone Flow Rate: 1.2 mL/min
4. Cortisone acetate Temperature: Ambient
5. 11-Ketoprogesterone 40 3 Detection: UV @ 254 nm
Sample: 1. Loratadine Related Compound A
2. Loratadine Related Compound B
14511
App ID 14511

3. Loratadine

App ID 19565
2 20
1
3
4
5

0 2 4 6 8 10 min 0 10 20 min

14 Phenomenex l WEB: www.phenomenex.com

Applications
SAW PALMETO BERRY, p-BROMOPHENACYL ESTERS USP METHOD: ACETAMINOPHEN

Column: Luna® 3 µm C8(2) Column: Luna 5 µm C18(2)

Dimension: 150 x 4.6 mm Dimension: 150 x 4.6 mm
Part No.: 00F-4248-E0 Part No.: 00F-4252-E0
Mobile Phase: Acetonitrile/Water (87:13) Mobile Phase: Water/Methanol/Acetic
Flow Rate: 1.5 mL/min 0.20000
Acid (69:28:3)
Temperature: 25 °C Flow Rate: 1.5 mL/min
Detection: UV @ 254 nm Temperature: 45 °C
Sample: 1. Capric acid Detection: UV @ 275 nm
2. Lauric acid Sample: 1. Acetaminophen
3. Linolenic acid 2. Caffeine
4. Myristic acid 3. Benzoic Acid

Absorbance
5. Linoleic acid 0.1000
6. Palmitic acid
7. Oleic acid

App ID 1072

App ID 1293
8. Stearic acid

0.0000

0 2 4 6 8 10 12 14 min

CYCLOSPORIN - IMMUNOSUPPRESSANTS CAPSAICIN

2
Column: Luna 5 µm C18(2) Column: Luna 5 µm C18(2)
Dimension: 150 x 4.6 mm 3 Dimension: 150 x 4.6 mm
Part No.: 00F-4252-E0 Part No.: 00F-4252-E0
Mobile Phase: Acetonitrile/Water, Mobile Phase: A: Acetonitrile/Water (35:65)
pH 3.1 w/1 mM H3PO4 (70:30) B: Acetonitrile/Water (60:40)
1
Flow Rate: 1.3 mL/min Gradient: 100 % A in 1 min to
Temperature: 75 °C 100 % B in 29 min
Detection: UV @ 210 nm Flow Rate: 1.5 mL/min
Sample: 1. Cyclosporin A Temperature: 75 °C
2. Cyclosporin D Detection: UV @ 227 nm
Sample: 1. Nordihydrocapsaicin
2 1 2. Capsaicin

App ID 1076
3. Dihydrocapsaicin
4. Homocapsaicin
App ID 1108

0 5 10 15 20 25 30 35 min 0 10 20 min

Phenomenex l WEB: www.phenomenex.com 15

 Luna PFP(2) ®

Powerful Selectivity for Reversed Phase Methods

Luna PFP(2) columns provide unique selectivity for highly polar compounds, complex natural products, Halogens can radically increase the polarity of compounds, thus decreasing typical retention characteristics. Luna
isomers and other closely related compounds. This is achieved by using a propyl-linked pentafluorophenyl, PFP(2) columns retain, discriminate, and separate halogens easily.
which provides multiple retention mechanisms unique to typical reversed phase medias.

POSITIONAL ISOMERS OF HALOGENATED PHENOLS

Luna PFP(2) selectivity is achieved through
4 mechanisms of interaction Luna 3 µm PFP(2)
5
Column: Luna 3 µm PFP(2)
1 Hydrogen Bonding Dimension: 150 x 4.6 mm
4 Part No.: 00F-4447-E0
2 Dipole-Dipole Interactions 2
3
Mobile Phase: A: 0.1 % Formic acid in Water

!
1 B: 0.1 % Formic acid in Acetonitrile
3 Aromatic and π-π Interactions 9 Gradient: A/B (60:40) to (50:50) in 10 min
Flow Rate: 1 mL/min
baseline
4 Hydrophobic 8 Temperature: 22 °C
6 separation
7 Detection: UV @ 254 nm
Sample: 1. 2,3-Dimethylphenol
2. 2,5-Dimethylphenol

App ID 16296
3. 2,6-Dimethylphenol
4. 3,4-Dimethylphenol
5. 3,5-Dimethylphenol
6. 2,5-Dichlorophenol
7. 2,6-Dichlorophenol
8. 3,4-Dichlorophenol
9. 3,5-Dichlorophenol
0 2 4 6 8 10 12 14 min 10. 2,4-Dibromophenol

Luna 3 µm C18(2)
16297
Column: Luna 3 µm C18(2)
5
Dimension: 150 x 4.6 mm

 Achieve unique selectivity using four mechanisms of solute/stationary phase interactions

1
2
3
4
!
coelution
Part No.:
Mobile Phase:
00F-4251-E0
A: 0.1% Formic acid in Water
B: 0.1% Formic acid in Acetonitrile
9 & 10 Gradient: A/B (60:40) to (50:50) in 10 min
 Extremely discerning for halogenated, aromatic, and conjugated compounds Flow Rate: 1 mL/min
8 Temperature: 22 °C
 Provides orthogonal selectivity, even using traditional reversed phase solutions 6
Detection: UV @ 254 nm
7
Sample: 1. 2,3-Dimethylphenol

App ID 16297
2. 2,5-Dimethylphenol
3. 2,6-Dimethylphenol
4. 3,4-Dimethylphenol
5. 3,5-Dimethylphenol
6. 2,5-Dichlorophenol
7. 2,6-Dichlorophenol
8. 3,4-Dichlorophenol
9. 3,5-Dichlorophenol
10. 2,4-Dibromophenol
0 2 4 6 8 10 12 14 min

16 Phenomenex l WEB: www.phenomenex.com

Isomeric Compounds Aromatic Compounds
Positional changes on an analyte of interest may effect the compound’s dipole moment. This change can be readily Aromatic compounds show unique retention characteristics on Luna PFP(2) compared to traditional reversed phase
seen by the way the highly electronegative fluorine (F) atoms and other retention mechanisms of the Luna PFP(2) columns. The presence of the aromatic benzene ring in Luna PFP(2) increases the relative attraction between the
are able to separate positional isomers. stationary phase and aromatic analytes, leading to increased retention for these types of compounds.

POSITIONAL ISOMERS OF METHYLACETOPHENONE CATECHINS

Luna 3 µm PFP(2)
16298
Luna 3 µm PFP(2)
16294
2 1
Column: Luna 3 µm PFP(2) Column: Luna 3 µm PFP(2)
Dimension: 150 x 4.6 mm Dimension: 150 x 4.6 mm
Part No.: 00F-4447-E0 Part No.: 00F-4447-E0
Mobile Phase: Water/ Methanol (50:50) Mobile Phase: A: 0.1 % Formic acid in Water

! 3
Flow Rate:
Temperature:
Detection:
1 mL/min
22 °C
UV @ 254 nm
!
baseline
Gradient:
Flow Rate:
B: 0.1 % Formic acid in Acetonitrile
A/B (80:20) to (55:45) in 10 min
1 mL/min
4 mechanisms of Temperature: 22 °C
Sample: 1. o-Methylacetophenone resolution
interaction provide 2. m-Methylacetophenone Detection: UV @ 280 nm
good resolution of isomers 1
3. p-Methylacetophenone Sample: 1. Gallic acid
8

App ID 16294
7 2. Epigallo catechin
App ID 16298

3. Catechin
5 6 4. Epicatechin
5. Epigallocatechin gallate
4
3 6. Gallocatechin gallate
2 7. Epicatechin gallate
8. Catechin gallate

0 2 4 6 8 10 12 14 16 18 min
0 2 4 6 8 10 12 14 min

Luna 3 µm C18(2) Luna 3 µm C18(2)

16295
16299 1
1
Column: Luna 3 µm C18(2)
Dimension: 150 x 4.6 mm

! Column: Luna 3 µm C18(2) Part No.: 00F-4251-E0

Dimension: 150 x 4.6 mm Mobile Phase: A: 0.1 % Formic acid in Water
B: 0.1 % Formic acid in Acetonitrile
Part No.: 00F-4251-E0
1 mechanism of Gradient: A/B (80:20) to (55:45) in 10 min
3 Mobile Phase: Water/ Methanol (50:50)
interaction unable to Flow Rate: 1 mL/min
Flow Rate: 1 mL/min
resolve isomers Temperature: 22 °C
Temperature: 22 °C
Detection: UV @ 280 nm
Detection: UV @ 254 nm

App ID 16295
Sample: 1. Gallic acid
App ID 16299

2 Sample: 1. o-Methylacetophenone 2. Epigallo catechin

2. m-Methylacetophenone 3. Catechin
3. p-Methylacetophenone 4. Epicatechin
56
5. Epigallocatechin gallate
3 4
6. Gallocatechin gallate
2 7. Epicatechin gallate
8. Catechin gallate

0 2 4 6 8 10 12 14 16 18 min 0 2 4 6 8 10 12 14 min

Phenomenex l WEB: www.phenomenex.com 17

 Luna Phenyl-Hexyl
Engineered for Stability
Luna Phenyl-Hexyl is a reproducible, extremely stable phenyl phase.
Most other phenyl phases use a short propyl (3 carbon) linker, which
limits the phase stability. This Luna phase uses a hexyl (6 carbon) linker
ANTIBACTERIALS: COMPARISON OF PHENYL COLUMNS
to attach the phenyl group to the silica surface.

The result:
Phenomenex Luna® 5 µm Phenyl-Hexyl
 Highly reproducible phenyl phase
 Dual selectivity of both phenyl phase and Conditions same for all columns
Dimension: 150 x 4.6 mm
a short alkyl phase (such as a C8)
Mobile Phase: A: 20 mM KH2PO4, pH 2.5
 Excellent retention of amine and polar aromatic compounds 6
B: Acetonitrile
Gradient: A/B (80:20) to A/B (75:25) in 5 min, then to A/B (55:45) in 15 min
 1.5 to 10 pH stability for 10,000 hours
7
Flow Rate: 1.0 mL/min
Detection: UV @ 254 nm
Temperature: 22 °C
3 Sample: 1. Carbadox
2. Thiamphenicol

App ID 1201
2
5 8
1
3. Furazolidone
4
4. Oxolinic acid
5. Sulfadimethoxine
6. Sulfaquinoxaline
7. Nalidixic acid
8. Piromidic acid

5 10 15 20 min

Waters® Spherisorb® 5 µm Phenyl Agilent Technologies® ZORBAX® 5 µm SB-Phenyl Agilent Technologies® ZORBAX® 5 µm Phenyl

5,6
5,6
5,6

3 Peaks 1,2 - Poor resolution 7

Peaks 4,5,6 - Poor Resolution
2 3
1 4 3
Peaks 7,8 1,2
App ID 10822

4 2

App ID 10821
Some absorption 4

App ID 10823
1
8
Peaks 7,8 - Total absorption

5 10 15 20 min
2.5 5.0 7.5 10.0 12.5 15.0 17.5 20.0 min 2.5 5.0 7.5 10.0 12.5 15.0 17.5 20.0 min

Agilent Technologies and ZORBAX are registered trademarks of Agilent Technologies. Spherisorb and Waters are registered trademarks of Waters Corp.
Phenomenex is not affiliated with Agilent Technologies or Waters Corp. Comparative separations may not be representative of all applications.

18 Phenomenex l WEB: www.phenomenex.com

Applications
COUGH AND COLD-USP METHOD Column: Luna® 5 µm Phenyl-Hexyl FOOD ADDITIVES Column: Luna 5 µm Phenyl-Hexyl
Dimension: 150 x 4.6 mm Dimension: 150 x 4.6 mm
Part No.: 00F-4257-E0 Part No.: 00F-4257-E0
Mobile Phase: Methanol/Water with 0.1 % H3PO4 Mobile Phase: A: 50 mM KH2PO4 + 0.1% H3PO4
and 0.1 % Heptane Sulfonate/ B: Acetonitrile
Acetonitrile with 0.1 % H3PO4 (35:55:10) Gradient: A/B (75:25) to A/B (25:75) in 18 min,
Flow Rate: 2.05 mL/min hold at A/B (25:75) for 12 min
Detection: UV @ 214 nm Flow Rate: 1.0 mL/min
Temperature: 22 °C Detection: UV @ 230 nm
Sample: 1. Acetaminophen Temperature: 22 °C
2. Pseudoephedrine Injection: 20 µL
3. Chlorphenaramine
4. Dextromethorphan Sample: 1. Saccharin
2. p-Hydroxybenzoic acid
3. Sorbic acid
4. p-Hydroxbenzoic acid methyl ester
5. Dehydroacetic acid
6. p-Toluic acid
7. p-Hydroxybenzoic acid ethyl ester

App ID 2575
8. n-Propyl p-hydroxybenzoate

App ID 1199
CHLOROBENZENES Column: Luna 5 µm Phenyl-Hexyl COUGH AND COLD MEDICINE Column: Luna 3 µm Phenyl-Hexyl
Dimension: 150 x 4.6 mm Dimension: 75 x 4.6 mm
Part No.: 00F-4257-E0 Part No.: 00C-4256-E0
Mobile Phase: A: Water Mobile Phase: A: Acetonitrile
B: Acetonitrile B: 20 mM KH2PO4 / Methanol(80:20) pH 9.0
Gradient: A/B (60:40) to A/B Gradient: A/B (0:100) to A/B (80:20) in 5 min
(45:55) in 10 min
Flow Rate: 1.0 mL/min
Flow Rate: 1.0 mL/min
Detection: UV @ 214 nm
Detection: UV @ 254 nm
Temperature: 22 °C
Temperature: 22 °C
7
Injection: 20 µL
Sample: 1. Chlorobenzene
2. 1,2-Dichlorobenzene 3 Sample: 1. p-Aminophenol
mAU 2
2. Benzoic acid
3. 1,4-Dichlorobenzene
3. Acetaminophen
4. 1,2,3-Trichlorobenzene 350
4. Pseudoephedrine
5. 1,3,5-Trichlorobenzene
5. Butyl paraben
6. 1,2,3,4-Tetrachlorobenzene 300
4 6. Chlorphenaramine
7. Diphenhydramine
250
8. Dextromethorphan
200
5
150

App ID 2572
App ID 1200

100

8
50 6
1

1 2 3 4 5 6 min

Phenomenex l WEB: www.phenomenex.com 19

 Luna (CN) Cyano
Proven Reproducibility Batch-to-Batch Reproducibility Column-to-Column Reproducibility
Column: Luna 5 µm CN Column: Luna 5 µm CN
Luna CN columns were developed to provide reproducibile Dimension: 150 x 4.6 mm Dimension: 150 x 4.6 mm
chromatography from run-to-run, column-to-column and batch-to- Mobile Phase: A: Hexane, B: Methylene chloride/ Mobile Phase: A: Hexane, B: Methylene
batch. Luna® high-purity silica provides a ridged and dense column Methanol(80:20), A/B (80:20) chloride/Methanol(80/20), A/B (95:5)

bed that allows for improved CN bonding techniques to make a stable Flow Rate: 2.0 mL/min Flow Rate: 1.0 mL/min
Detection: UV @ 254 nm Detection: UV @ 254 nm
CN phase.
Injection: 1.0 µL Injection: 5.0 µL
Temperature: Ambient Temperature: Ambient
The result: Sample: 1. Hydrocortisone Sample: 1. Dimethyl phthalate
2. Prednisone 2. Diethyl phthalate
 One of the most stable CN columns under both reversed 3. Cortisone
4. Hydrocortisone acetate
3. Dibutyl phthalate
4. Dioctyl phthalate
phase or normal phase conditions.
 Reproducible from run-to-run, column-to-column,
batch-to-batch.
 pH stable from 1.5 to 7.0

App ID 3464

App ID 3466
Batch 1 Column 1
Batch 2 Column 2
Batch 3 Column 3

PHTHALATE ESTERS: A COMPARSION OF CN COLUMNS

Waters® Spherisorb® 5 µm CN

Phenomenex Luna 5 µm CN 1
2
3
4+5
6

0 1 2 3 4 5 6 7 8 9 min
mi

Agilent Technologies® ZORBAX® 5 µm SB-CN Conditions same for all columns

Dimension: 150 x 4.6 mm
4 6 5 Mobile Phase: A: Hexane, B: Methylene
3 6 chloride/Methanol (80:20), A/B (99:1)
3 4
5
1 1 2 Flow Rate: 1.0 mL/min
2
Detection: UV @ 254 nm
Temperature: Ambient
App ID 3471

Sample: 1. Di-n-octyl phthalate

2. Bis (2-Ethylhexyl) phthalate
3. Butylbenzyl phthalate
4. Di-n-butyl phthalate
5. Diethyl phthalate
6. Dimethyl phthalate
0 1 2 3 4 5 6 7 8 9 min
0 1 2 3 4 5 6 7 8 9 min

4+5
Agilent Technologies and ZORBAX are registered trademarks
1 of Agilent
3 Technologies. Waters and Spherisorb are registered trademarks of Waters Corporation. Phenomenex is not affiliated with Agilent Technologies or Waters Corporation.
2 6
The comparative data presented here may not be representative for all applications.

20 Phenomenex l WEB: www.phenomenex.com

Luna (NH2) Amino
Developed for Ruggedness NUCLEIC ACID BASES Column: Luna 5 µm NH2
Dimension: 150 x 4.6 mm
Part No.: 00F-4378-E0
Luna® NH2 columns were developed to provide improved amino column lifetime. Column life for most amino Mobile Phase: Acetonitrile/Water (80:20)
columns can be problematic as the amino bonding easily strips off the silica. Luna NH2 columns, however, Flow Rate: 1.0 mL/min
show good bonded phase stability under both normal and reversed phase modes and across a pH range of 2 Detection: UV @ 254 nm

1.5 to 11.0. Such a broad pH range indicates the bonded phase ruggedness and the density of the bonded Temperature: 40 °C
Sample: 1. Thymine
phase coverage. 1
2. Uracil
4 3. Cytosine
4. Adenine
The result: 3 5. Guanosine

 Long lifetimes and low phase bleed for more reproducible methods 5

APP ID 14302
 Excellent retention of simple sugars, complex sugars, sugar alcohols by reverse phase conditions and
hydrogen bonding compounds under normal phase conditions
 pH stable from 1.5 to 11.0
 Stable in 100 % aqueous mobile phases
1.0 2.0 3.0 4.0 5.0 min

SIMPLE SUGARS Column: Luna 5 µm NH2 STEROIDS Column: Luna 5 µm NH2

Dimension: 250 x 4.6 mm Dimension: 250 x 4.6 mm
Part No.: 00G-4378-E0 Part No.: 00G-4378-E0
Mobile Phase: Acetonitrile/Water (80:20) Mobile Phase: Hexane/Ethanol (85:15)
Flow Rate: 3.0 mL/min Flow Rate: 2.0 mL/min
Detection: RI Detection: UV @ 240 nm
Temperature: 40 °C Temperature: 22 °C
Sample: 1. Xylose Sample: 1. 11-Ketoprogesterone
2. Fructose 2. 11-Hydroxyprogesterone
3. Glucose 3. Cortisone acetate
4. Sucrose 4. Prednisolone 21-acetate
5. Maltose 5. Cortisone
6. Melezitose 6. Prednisolone
7. Raffinose 3
2

5
4
4
6
3 2
APP ID 14285

APP ID 14299
5 6
7 1
1

0 2 4 6 8 10 12 min 0 2 4 6 8 10 12 min

Phenomenex l WEB: www.phenomenex.com 21

 Luna HILIC
Increase MS-Sensitivity and Retention for Polar Compounds

Luna® HILIC columns retain a water-enriched layer on the surface of the silica. This water layer facilitates the transfer of Separation is achieved through the partitioning of polar solutes from the high concentration, water-
polar compounds into the stationary phase for increased retention. miscible, organic mobile phase into the hydrophilic surface environment. Polar solutes exhibit increased
retention, and elute in the order of increasing hydrophilicity.
The result:
 Superior retention of polar compounds
 Improve mass spec sensitivity
 Increased laboratory throughput and productivity

Improve Mass Spec Sensitivity

Luna HILIC columns allow low level polar metabolites to be retained on column past the critical ion suppression zone, allowing: Increased MS sensitivity and Higher signal-to-noise ratio (S/N).

POLAR COMPOUND IN HILIC MODE

S/N = 20.6

Great MS sensitivity Column: Luna 3 µm HILIC

Dimension: 100 x 2.0 mm
Part No.: 00D-4449-B0
Mobile Phase: Acetonitrile / 100 mM Ammonium
Formate, pH 3.2 (90:10)
Flow Rate: 0.4 mL/min
Detection: Mass Spectrometer (MS)
Temperature: Ambient
Primary Ion Sample: Bamethan
Suppression Region

App ID 16443
0 min
to 1 2

POLAR COMPOUND IN C18 REVERSED PHASE

Ion Suppression Region is
from 0.5-1.0 min
S/N = 6.2 Column: Gemini 3 µm C18
Dimension: 100 x 2.0 mm
Part No.: 00D-4435-B0 ~ 0.5 min
to=
Mobile Phase: 0.1 % Formic Acid / Acetonitrile (97:3)
Poor MS Flow Rate: 0.4 mL/min tR–to 1- 0.5
sensitivity Detection: Mass Spectrometer (MS) k'=1= =
Temperature: to
App ID 16444

Ambient
Sample: Bamethan 0.5

0
to 1 2 min

22 Phenomenex l WEB: www.phenomenex.com

Retain Polar Compounds Unique HILIC Selectivity
Highly polar compounds such as ribavirin may be poorly retained on reversed phase columns. Not all HILIC columns are alike. Luna HILIC columns deliver on the exacting standards you’ve come to trust from
HILIC techniques will increase polar compound retention and sensitivity. the Luna product line.

RIBAVIRIN ON LUNA HILIC

0.5 ng on column HILIC COLUMN COMPARISON
1 2

Phenomenex Luna® 5 µm HILIC

1
Column: Luna 3 µm HILIC 5
Dimension: 100 x 2.0 mm to
Part No.: 00D-4449-B0 4
Requires Mobile Phase: A: Acetonitrile/100 mM Ammonium
6
gradient Formate, pH 3.2 (90:10)
Increased retention and to elute
3
B: Acetonitrile/20 mM Ammonium Formate,
sensitivity pH 3.2 (50:50)
1 2 Baseline
0 2 4 6 8 10 min
Peak area = 20,759 Gradient: 100 % A for 3 min, then 100 % B to 4.5 5 resolution of

App ID 16337
min, switch to 100 % A for 10 min to all peaks
Flow Rate: 0.4 mL/min 4
App ID 16442

6
Detection: Mass Spectrometer (MS)
Temperature: Ambient 1 2
3
Sample: 1. Ribavirin (MRM: 245.2/113.2)
to 5
0 2 4 6 8 10 min
4

0 0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8 2.0 2.2 2.4 2.6 2.8 min 3

Waters ® 1 ®2 5
Atlantis μm HILIC 4
0 2 6 8 10 min

to 5

1
2

App ID 16432
4

to 3
RIBAVIRIN ON C18 1 2
5
6 8 4
0 2 4 6 10 min
5 3 (Thiamine-no elution)
0.5 ng on column
to
1 4
1 0 2 2 4 66 8 10 min
Column: Gemini® 5 µm C18
Elutes at Dimension: 100 x 2.0 mm to
void peak Part No.: 00D-4435-B0 3
5
area = 17,996 Mobile Phase: Acetonitrile with 0.1 % v/v Formic Acid/ 4
0 2 4 6 8 10 min
Water with 0.1 v/v % Formic Acid (3:97) 3
Flow Rate: 0.4 mL/min Conditions same for all columns:
SeQuant 5 μm ZIC®-HILIC
Detection: Mass Spectrometer (MS) (ambient) 0 2 4 6 8 10 min Column: As noted
Temperature: Ambient Dimension: 150 x 4.6 mm
Sample: 1. Ribavirin (MRM: 245.2/113.2) 1 2 Mobile Phase: Acetonitrile/100 mM

App ID 16431
Ammonium Formate,
5
App ID 16343

to pH 3.2 (90:10)
Flow Rate: 1.0 mL/min
4 6 Detection: UV @ 260 nm
k’ = 0.85
3 (Thiamine-no elution) Sample: 1. PABA
2. Nicotinamide
0 2 4 6 8 10 min 3. Riboflavin
0 0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8 2.0 2.2 2.4 2.6 2.8 min 4. Nicotinic Acid
5. Pyridoxine
1 6. Thiamine
2

to
ZIC is a registered trademark of Merck SeQuant AB. Waters and Atlantis are registered trademarks of Waters Corporation. Phenomenex is not
5
affiliated with Merck SeQuant AB or Waters Corporation. The comparative data presented
4 here may not be representative for all applications.
3

0 2 4 6 8 10 min

Phenomenex l WEB: www.phenomenex.com 23

 Luna SCX
Develop Robust Strong Cation Exhange Methods

Luna® SCX columns provide excellent resolution and peak shape of basic, cationic compounds. However, most Luna SCX columns contain a benzene sulfonic acid ligand providing ion exchange reversed phase, and aromatic
SCX columns show poor peak shape and bad resolution causing many chromatographers to ignore this important interactions. In combination with the ultra pure silica, Luna SCX columns are a stable, robust phase for strong cation
phase for small molecule method development, until now. exchange chromatography.

The result:
 Resolving power and sharp peak shape to separate complex cationic/basic
and nitrogen containing compounds
 Benzene sulfonic acid ligand provides mixed-mode interaction improving separation
for 2D peptide applications
 Excellent first dimension of 2D LC applications

PEPTIDES TRYPTIC DIGEST OF BOVINE CYTOCHROME c

Column: Luna 5 μm SCX Column: Luna 5 μm SCX
Dimension: 150 x 4.6 mm Dimension: 150 x 4.6 mm
Part No.: 00F-4398-E0 Part No.: 00F-4398-E0
Mobile Phase: A: 20 mM Potassium Phosphate, Mobile Phase: A: 20 mM Potassium Phosphate,
25 % Acetonitrile, pH 2.5 pH 2.5 /25 % Acetonitrile
B: 20 mM Potassium Phosphate, B: 20 mM Potassium Phosphate,
25 % Acetonitrile, 400 mM pH 2.5 /25 % Acetonitrile
1 Potassium Chloride, pH 2.5 / 350 mM Potassium Chloride
2 Gradient: A/B (95:5) to A/B (10:90) in 45 min Gradient: 100 % A to 100 % B in 50 min
6
Flow Rate: 1 mL/min Flow Rate: 1 mL/min
5
Temperature: 35 °C Temperature: 35 °C
3
4 Detection: UV @ 215 nm Detection: UV @ 215 nm
Injection Volume: 2 µL (5 µg on column) Injection Volume: 50 µL (20 µg on column)

App ID 14767
App ID 14765

Sample: Peptide Mixture - Substance P Sample: Bovine Cytochrome c trypsin digest

1. Fragment 5-11 (+1)
2. Fragment 4-11 (+1)
3. Fragment 2-11 (+2)
4. Fragment 1-9 (+3)
5. Intact (+3)
0 5 10 15 20 25 30 35 40 min 6. (ARG-PHE-TRP-LEU) (+3) 0 5 10 15 20 25 30 35 40 45 min

24 Phenomenex l WEB: www.phenomenex.com

 AXIA
™

Revolutionize Lab-Scale Purification

An advanced column packing and hardware design, Axia columns incorporate patented With Axia technology, the correct slurry amount and packing pressure are automated to
Hydraulic Piston Compression technology to eliminate bed collapse as a source of failure give not only higher efficiency and sharper peaks, but also drastically reduced column-
in preparative columns. Using a single, controlled hydraulic compression, the piston to-column variability. This will help improve longer column lifetime, column-to-column
assembly is locked in place without allowing the media to decompress or “relax,” thus reproducibility, and recover higher compound purity with analytical like efficiency.
maintaining media and column bed integrity.

Axia Gradient Lifetime Study Compare Lifetime

mAu
20000
2 1 2 3 AxiamAu
Packed 1 2 3

Luna® 5 µm C18(2) Axia Packed

500 First
500 Run 800th Run

0 0

2 3 4 min 2 3 4
10000

Overlay of 1, 800, 1300

3

Cycles:
vs. Leading Competitive Preparative Column
Waters® XTerra® 5 µm Prep MS C18 OBD™
chromatograms 1
1300
mAu mAu
First Run 120th Run*
1 2 3800 1 2 3
*Six columns tested.
Best lifetime chosen for comparison.
0 1

500 500 Conditions same for both columns except where noted
Column: Luna 5 µm C18(2) Axia Packed
Waters XTerra 5 µm Prep MS C18 OBD
0 1 2 3 4 min Dimension: 50 x 21.2 mm (Luna)
50 x 19 mm (XTerra)
Mobile Phase: A: 0.5 % TFA in Water
Columns: Luna 5 µm C18(2) Axia Packed
0 0
B: 0.5 % TFA in Acetonitrile
Dimension: 50 x 21.2 mm
Gradient: Linear 95:5 (A/B) to 5:95 (A/B) over 7 min,
Part No.: 00B-4252-P0-AX 2 3 4 min 2 3 4 min hold 3 min
Mobile Phase: A: 0.5% TFA in Water Flow Rate: 30 mL/min (Luna)
B: 0.5% TFA in Acetonitrile 24 mL/min (XTerra)‡
Gradient: Linear 95:5 to 5:95 (A/B) over 7 min, hold 3 min Temperature: Ambient
Injection Volume: 500 μL Detection: UV @ 254 nm
Flow Rate: 30 mL/min Waters and XTerra are registered trademarks of Waters Corporation. OBD is a trademark of Sample: 1. Triprolidine 1.6 mg
Temperature: Ambient 2. Methacycline 16 mg
Waters Corporation. Phenomenex is not affiliated with Waters Corporation. The comparative data 3. Amitriptyline 5.25 mg
Detection: UV @ 254 nm presented here may not be representative for all applications. ‡
Same linear velocity
Sample: 1. Triprolidine 1.6 mg
2. Methacycline 16 mg
3. Amitriptyline 5.25 mg 2006 R&D 100 Award Recipient For more detailed information on Axia Preparative columns visit: www.phenomenex.com/axia

Phenomenex l WEB: www.phenomenex.com 25

 Fast LC
When you want Fast LC, you need BALANCE

The ever-increasing demand for high-throughput analysis of drug candidates during the
early stages of drug discovery has generated an acute need for rapid methods of analysis.

3 Balanced Solutions to Balance Your Speed: Pressure, Efficiency and Selectivity HST Columns: 66 % Faster. No Loss in Resolution

Luna 5 μm C18(2) 250 x 4.6 mm

Speed Pressure Efficiency Selectivity Flow Rate: 1.5 mL/min
Fast Several phases

App ID 16281
1 High Speed Technology (HST) Columns < 400 Bar Highest
Run Time available RS = 24

Fastest Most phases

2 MercuryMS™ Columns and Cartridges < 400 Bar High
Run Time available 0 10 20 30 min

Fast Several phases

3 Monolithic Columns < 200 Bar Good
Run Time available

Developing ultra-fast and efficient methods for potential drugs has become a constant challenge for Luna 3 μm C18(2) 150 x 4.6 mm
analysts. Use the chart above to determine the HPLC column that meets your performance needs. Flow Rate: 1.5 mL/min
RS = 24

App ID 16274
Luna: High Speed Technology (HST) columns

 High efficiency 2.5 µm particles on ultra-pure silica 0 10 20 min

 Ultra-high performance results on your current HPLC

 Easy method transfer
 Orthogonal selectivity options
Luna 2.5 μm C18(2)-HST 100 x 2.0 mm
Flow Rate: 0.65 mL/min Run time reduced
by 20 min
with virtually no effect
RS = 23
on resolution!

App ID 16201
Luna HST columns are manufactured in specific dimensions utilizing new, highly
controlled and robust packing technologies. The technology allows for consistent,
high performance results on newer and existing HPLC instrumentation. Get the benefit 0 5 10 min
of increased speed and efficiency with standard HPLC system pressure capabilities!
Conditions for all columns:
Luna HST can be used with your current standard HPLC and newer high performance
Column: Luna C18(2), particle size as noted
systems so that there will be no need for time consuming method revalidation.
Dimension: as noted
Mobile Phase: A: Water B: Acetonitrile
Luna HST 2.5 µm columns allow the scientist to reduce analysis time by increasing flow Gradient: 90:10 (A/B) to 5:95 (A/B)
rates without a loss in performance. Flow Rate: As noted
Detection: UV @ 270 nm
Sample: Ketones C3 to C16

26 Phenomenex l WEB: www.phenomenex.com

MercuryMS™: Columns and Cartridges Rugged Durability for Over 1,000 Injections

 Ultra-fast, low-cost analysis for high-throughput laboratories When running long sequences, often involving hundreds of valuable samples, it is imperative that the column does
not fail during the middle of the run. As shown, the Luna 3 μm C18(2) 20 x 2.0 mm MercuryMS cartridge was stable
 Packed with Luna , Synergi , and Gemini material
® ® ®
for over 1000 injections (over 20,000 column volumes of mobile phase) in this gradient assay, offering exceptional
 Short 10 and 20 mm cartridge formats use a new proprietary slurry packing process reliability for automated screening systems.

Reduce Analysis Times by 60 %

With the increasing emphasis on high sample throughput for screening combinatorial libraries as well as the
need for overall faster cycle time, it has become necessary for the chromatographer to reduce analysis time
while still maintaining acceptable resolution. As shown, retention times can be significantly reduced with a 20 MercuryMS: Stable Over 1,000 Injections
x 4.0 mm MercuryMS cartridge column.
3.00
Column: Luna 3 μm C18(2) 20 x 2.0 mm
MercuryMS Cartridge
2.75
60% Reduction in Analysis Time Using MercuryMS Part No.: 00M-4251-B0-CE
Mobile Phase: A: Water with 0.1 %
2.50
Trifluoroacetic acid (TFA)
50 x 4.6 mm Column B: Methanol with 0.1 % TFA
Gradient: 95:5 A/B to 5: 95 A/B in 2 min
2.25
at a flow rate of 0.4 mL/min, then
App ID 10849

reequilibrate for 3 min at 1.0 mL/min

Retention time (min) or Symmetry

Flow Rate: 0.4 and 1.0 mL/min
tR #1
2.00 Detection: UV @ 230 nm
tR #2
Temperature: Ambient
tR #3 Sample: 2 µL containing:
1.75 1. Propranolol
Symmetry #1
0 2 4 min 2. Metoprolol

vs.
Symmetry #2 3. Pindolol
30 x 4.6 mm Column 1.50
Symmetry #3

1.25
App ID 10850

Column: Luna 3 μm C18(2)

Dimension: 50 x 4.6 mm, 30 x 4.6 mm,
20 x 4.0 mm 1.00
Mobile Phase: A: 0.1 % Formic acid in Water
B: 0.1 % Formic acid Acetonitrile
Gradient: 95:5 A/B to 5:95 in 6 min 0.75
0 2 4 min
for 50 x 4.6 mm
95:5 A/B to 5:95 in 4 min

vs.
for 30 x 4.6 mm 0.50
20 x 4.0 mm Cartridge 95:5 A/B to 5:95 in 2.4 min 0 200 400 600 800 1000
for 20 x 4.0 mm
Injection#
60 % Reduction Flow Rate: 2 mL/min for 50 and 30 x 4.6 mm,
1.6 mL/min for 20 x 4.0 mm
in Run Time
Detection: UV @ 254 nm
App ID 10851

Temperature: Ambient
Sample: 5 µL Gradient mixture
1. Acetaminophen
2. Propranolol
3. Imipramine
0 2 4 min 4. Naproxen
5. Valerophenone

Phenomenex l WEB: www.phenomenex.com 27

 Ordering Information
2.5 μm High Speed Technology (HST) Columns (mm)
30 x 2.0 50 x 2.0 100 x 2.0 50 x 3.0 100 x 3.0
Phases
Luna 2.5 µm C18(2)-HST 00A-4446-B0 00B-4446-B0 00D-4446-B0 00B-4446-Y0 00D-4446-Y0

3 μm and 5 μm Capillary Columns (mm) Guard Columns (mm)

50 x 0.30 150 x 0.30 250 x 0.30 50 x 0.50 150 x 0.50 250 x 0.50 20 x 0.30 20 x 0.50
Phases
3 μm C8(2) 00B-4248-AC 00F-4248-AC 00G-4248-AC
–– 00B-4248-AF 00F-4248-AF 00G-4248-AF
–– 03M-4248-AC 03M-4248-AF
3 μm C18(2) 00B-4251-AC 00F-4251-AC 00G-4251-AC 00B-4251-AF 00F-4251-AF 00G-4251-AF 03M-4251-AC 03M-4251-AF
5 μm C8(2) 00B-4249-AC
–– 00F-4249-AC 00G-4249-AC 00B-4249-AF
–– 00F-4249-AF 00G-4249-AF
–– 03M-4249-AC 03M-4249-AF
5 μm C18(2) 00B-4252-AC 00F-4252-AC 00G-4252-AC 00B-4252-AF 00F-4252-AF 00G-4252-AF 03M-4252-AC 03M-4252-AF
5 μm Phenyl-Hexyl 00B-4257-AC 00F-4257-AC
–– –– 00B-4257-AF 00F-4257-AF
–– –– –– ––

Cartridge Holders
MercuryMS™ LC/MS Cartridges (mm)
10 x 2.0 10 x 4.0 20 x 2.0 20 x 4.0
Phases

3 μm
C18(2) 00N-4251-B0-CE 00N-4251-D0-CE 00M-4251-B0-CE 00M-4251-D0-CE
C8(2) 00N-4248-B0-CE 00N-4248-D0-CE 00M-4248-B0-CE 00M-4248-D0-CE Standard Cartridge Holders Direct-Connect Cartridge Holders
5 μm Part No. Description Price Part No. Description Price
C18(2) 00N-4252-B0-CE –– 00M-4252-B0-CE 00M-4252-D0-CE CH0-5846 10 mm standard holder CH0-7187 10 mm direct-connect holder
C8(2) 00N-4249-B0-CE –– 00M-4249-B0-CE 00M-4249-D0-CE CH0-5845 20 mm standard holder CH0-7188 20 mm direct-connect holder

SecurityGuard™ Analytical Cartridges require universal holder Part No.: KJO-4282

SecurityGuard
3 μm Microbore and Minibore Columns (mm) Cartridges (mm)
50 x 1.0 150 x 1.0 30 x 2.0 50 x 2.0 100 x 2.0 150 x 2.0 4 x 2.0
Phases 10/pk
Silica(2) — — 00A-4162-B0 00B-4162-B0 00D-4162-B0 00F-4162-B0 AJ0-4347
C8(2) 00B-4248-A0 00F-4248-A0 00A-4248-B0 00B-4248-B0 00D-4248-B0 00F-4248-B0 AJ0-4289
C18(2) 00B-4251-A0 00F-4251-A0 00A-4251-B0 00B-4251-B0 00D-4251-B0 00F-4251-B0 AJ0-4286
CN — — 00A-4254-B0 00B-4254-B0 00D-4254-B0 00F-4254-B0 AJ0-4304
Phenyl-Hexyl 00B-4256-A0 — 00A-4256-B0 00B-4256-B0 00D-4256-B0 00F-4256-B0 AJ0-4350
NH2 — 00F-4377-A0 00A-4377-B0 00B-4377-B0 00D-4377-B0 00F-4377-B0 AJ0-4301
HILIC — — — 00B-4449-B0 00D-4449-B0 00F-4449-B0 AJ0-8328
PFP(2) — — 00A-4447-B0 00B-4447-B0 00D-4447-B0 00F-4447-B0 AJ0-8326
for ID: 2.0-3.0 mm

28 Phenomenex l WEB: www.phenomenex.com

Ordering Information
SecurityGuard™ Analytical Cartridges require universal holder Part No.: KJO-4282

SecurityGuard
SecurityGuard™
3 μm Narrow
Analytical
Bore
Columns
and Analytical
(mm) Columns (mm) Cartridges (mm)
30 x 3.0 50 x 3.0 150 x 3.0 30 x 4.6 50 x 4.6 75 x 4.6 100 x 4.6 150 x 4.6 4 x 2.0 4 x 3.0
Phases 10/pk
/10 pk 10/pk
/10 pk
Silica(2) — — 00F-4162-Y0 — 00B-4162-E0 — 00D-4162-E0 00F-4162-E0 AJ0-4347 AJ0-4348
C8(2) 00A-4248-Y0 00B-4248-Y0 00F-4248-Y0 00A-4248-E0 00B-4248-E0 00C-4248-E0 00D-4248-E0 00F-4248-E0 AJ0-4289 AJ0-4290
C18(2) 00A-4251-Y0 00B-4251-Y0 00F-4251-Y0 00A-4251-E0 00B-4251-E0 00C-4251-E0 00D-4251-E0 00F-4251-E0 AJ0-4286 AJ0-4287
CN — 00B-4254-Y0 00F-4254-Y0 00A-4254-E0 00B-4254-E0 00C-4254-E0 00D-4254-E0 00F-4254-E0 AJ0-4304 AJ0-4305
Phenyl-Hexyl — 00B-4256-Y0 00F-4256-Y0 00A-4256-E0 00B-4256-E0 00C-4256-E0 00D-4256-E0 00F-4256-E0 AJ0-4350 AJ0-4351
NH2 — 00B-4377-Y0 00F-4377-Y0 00A-4377-E0
— 00B-4377-E0 — 00D-4377-E0 00F-4377-E0 AJ0-4301 AJ0-4302
HILIC — 00B-4449-Y0 00F-4449-Y0 — — — 00D-4449-E0 00F-4449-E0 AJ0-8328 AJ0-8329
PFP(2) — 00B-4447-Y0 00F-4447-Y0 — 00B-4447-E0 — 00D-4447-E0 00F-4447-E0 AJ0-8326 AJ0-8327
for ID: 2.0-3.0 mm 3.2-8.0 mm

SecurityGuard™ Analytical Cartridges require universal holder Part No.: KJO-4282

SecurityGuard
5 μm Microbore and Minibore Columns (mm) Cartridges (mm)
50 x 1.0 150 x 1.0 250 x 1.0 30 x 2.0 50 x 2.0 150 x 2.0 250 x 2.0 4 x 2.0* Contact your Phenomenex technical
Phases 10/pk consultant for bulk media sales.
Silica(2) — — — — 00B-4274-B0 00F-4274-B0 00G-4274-B0 AJ0-4347
C5 — — — 00A-4043-B0 00B-4043-B0 00F-4043-B0 — AJ0-4292
C8 (2) 00B-4249-A0 00F-4249-A0 — 00A-4249-B0 00B-4249-B0 00F-4249-B0 00G-4249-B0 AJ0-4289
C18 (2) 00B-4252-A0 00F-4252-A0 00G-4252-A0 00A-4252-B0 00B-4252-B0 00F-4252-B0 00G-4252-B0 AJ0-4286
CN — — — 00A-4255-B0 00B-4255-B0 00F-4255-B0 00G-4255-B0 AJ0-4304
Phenyl-Hexyl 00B-4257-A0 00F-4257-A0 00G-4257-A0 00A-4257-B0 00B-4257-B0 00F-4257-B0 00G-4257-B0 AJ0-4350
NH2 — 00F-4378-A0 — 00A-4378-B0 00B-4378-B0 00F-4378-B0 00G-4378-B0 AJ0-4301
PFP(2) — — — 00A-4448-B0 00B-4448-B0 00F-4448-B0 — AJ0-8326
for ID: 2.0-3.0 mm

SecurityGuard™ Analytical Cartridges require universal holder Part No.: KJO-4282

SecurityGuard
SecurityGuard™
5 μm Narrow
Analytical
Bore
Columns
and Analytical
(mm) Columns (mm) Cartridges (mm)
30 x 3.0 50 x 3.0 150 x 3.0 250 x 3.0 30 x 4.6 50 x 4.6 75 x 4.6 4 x 2.0* 4 x 3.0*
Phases /10
10/pk
pk /10
10/pk
pk
Silica(2) — — — — — 00B-4274-E0 — AJ0-4347 AJ0-4348
C5 — — 00F-4043-Y0 — — 00B-4043-E0 — AJ0-4292 AJ0-4293 If Luna analytical columns do not provide
you with at least equivalent separations
C8(2) — 00B-4249-Y0 00F-4249-Y0 00G-4249-Y0 00A-4249-E0 00B-4249-E0 00C-4249-E0 AJ0-4289 AJ0-4290 to a competing column of the same
C18(2) 00A-4252-Y0 00B-4252-Y0 00F-4252-Y0 00G-4252-Y0 00A-4252-E0 00B-4252-E0 00C-4252-E0 AJ0-4286 AJ0-4287 phase, particle size, and dimensions,
CN — 00B-4255-Y0 00F-4255-Y0 00G-4255-Y0 00A-4255-E0 00B-4255-E0 00C-4255-E0 AJ0-4304 AJ0-4305 return the column with comparative data
Phenyl-Hexyl 00A-4257-Y0 00B-4257-Y0 00F-4257-Y0 00G-4257-Y0 00A-4257-E0 00B-4257-E0 00C-4257-E0 AJ0-4350 AJ0-4351 within 45 days for a FULL REFUND.
NH2 — 00B-4378-Y0 00F-4378-Y0 00G-4378-Y0 00A-4378-E0 00B-4378-E0 00C-4378-E0 AJ0-4301 AJ0-4302
SCX — — — — — 00B-4398-E0 — AJ0-4307 AJ0-4308
HILIC — — 00F-4450-Y0 — — — — AJ0-8328 AJ0-8329
PFP(2) — 00B-4448-Y0 00F-4448-Y0 — 00A-4448-E0 00B-4448-E0 — AJ0-8326 AJ0-8327
for ID: 2.0-3.0 mm 3.2-8.0 mm

Phenomenex l WEB: www.phenomenex.com 29

 Ordering Information
Luna Method Development Kits
The Luna Method Development Kits combine the
incredible performance of Luna HPLC columns
with three different selectivities. Both kits include
5 μm Analytical, Semi-Prep and Preparative Columns (mm) SecurityGuard™ Cartridges (mm) a Luna Phenyl-Hexyl (moderate polarity), a Luna
100 x 4.6 150 x 4.6 250 x 4.6 250 x 10 250 x 15 4 x 3.0* 10 x 10‡ CN (polar), and your choice of a C8(2) or C18(2)
Phases 10/pk 3/pk (non-polar). A wide range of selectivies for virtually
Silica(2) 00D-4274-E0 00F-4274-E0 00G-4274-E0 00G-4274-N0 — AJ0-4348 AJ0-7223 any separation challenge.
C5 00D-4043-E0 00F-4043-E0 00G-4043-E0 — — AJ0-4293 AJO-7372
C8(2) 00D-4249-E0 00F-4249-E0 00G-4249-E0 00G-4249-N0 — AJ0-4290 AJ0-7222 Method Development Kits
C18(2) 00D-4252-E0 00F-4252-E0 00G-4252-E0 00G-4252-N0 00G-4252-AK AJ0-4287 AJ0-7221 Part No. Description Dimensions (mm) Unit Price
CN 00D-4255-E0 00F-4255-E0 00G-4255-E0 00G-4255-N0 — AJ0-4305 AJ0-7313 KH0-4760 Luna Method Development Kit (5 columns) 150 x 4.6 5/pk
Phenyl-Hexyl 00D-4257-E0 00F-4257-E0 00G-4257-E0 00G-4257-N0 — AJ0-4351 AJ0-7314 1 each of 5 µm C18(2), C8(2), C5, CN, Phenyl-Hexyl Phases
NH2 00D-4378-E0 00F-4378-E0 00G-4378-E0 00G-4378-N0 — AJ0-4302 AJ0-7364 KH0-4354 Luna Rapid-MD Kit (3 columns) 50 x 4.6 3/pk
SCX 00D-4398-E0 00F-4398-E0 00G-4398-E0 00G-4398-N0 — AJ0-4308 AJ0-7369 1 each of 3 µm C18(2), C8(2) and Phenyl-Hexyl phases
HILIC 00D-4450-E0 00F-4450-E0 00G-4450-E0 00G-4450-N0 — AJ0-8329 AJ0-8902 KH0-4761 Luna Selectivity Kit 1 (3 columns) 150 x 4.6 3/pk
PFP(2) 00D-4448-E0 00F-4448-E0 00G-4448-E0 00G-4448-N0 — AJ0-8327 AJ0-8376 1 each of 5 µm C18(2), Phenyl-Hexyl, CN phases
for ID: 3.2-8.0 mm 9-16 mm KH0-4762 Luna Selectivity Kit 2 (3 columns) 150 x 4.6 3/pk
1 each of 5 µm C8(2), Phenyl-Hexyl, CN phases

Axia™ Packed Preparative Columns (mm) SecurityGuard Cartridges (mm) 10 μm Analytical and Semi-Prep SecurityGuard Cartridges (mm)
50 x 21.2 100 x 21.2 150 x 21.2 250 x 21.2 50 x 30 100 x 30 250 x 30 15 x 21.2** 15 x 30 ◊ 250 x 4.6 250 x 10 4 x 3.0* 10 x 10‡
Phases ea ea Phases 10/pk 3/pk
5 μm Silica(2) 00G-4091-E0 00G-4091-N0 AJ0-4348 AJ0-7223
Silica(2) 00B-4274-P0-AX 00D-4274-P0-AX 00F-4274-P0-AX 00G-4274-P0-AX 00B-4274-U0-AX 00D-4274-U0-AX 00G-4274-U0-AX AJ0-7229 AJ0-8312 C5 00G-4092-E0 00G-4092-N0 AJ0-4293 AJ0-7372
C5 — — 00F-4043-P0-AX — — — — — — C8(2) 00G-4250-E0 00G-4250-N0 AJ0-4290 AJ0-7222
C8(2) 00B-4249-P0-AX 00D-4249-P0-AX 00F-4249-P0-AX — 00B-4249-U0-AX 00D-4249-U0-AX — AJ0-7840 AJ0-8302 C18(2) 00G-4253-E0 00G-4253-N0 AJ0-4287 AJ0-7221
C18(2) 00B-4252-P0-AX 00D-4252-P0-AX 00F-4252-P0-AX 00G-4252-P0-AX 00B-4252-U0-AX 00D-4252-U0-AX 00G-4252-U0-AX AJ0-7839 AJ0-8301 CN 00G-4300-E0 00G-4300-N0 AJ0-4305 AJ0-7313
CN 00B-4255-P0-AX — 00F-4255-P0-AX 00G-4255-P0-AX — 00D-4255-U0-AX — AJ0-8220 AJ0-8311 Phenyl-Hexyl 00G-4285-E0 00G-4285-N0 AJ0-4351 AJ0-7314
Phenyl-Hexyl 00B-4257-P0-AX 00D-4257-P0-AX 00F-4257-P0-AX — 00B-4257-U0-AX 00D-4257-U0-AX — AJ0-7841 AJ0-8303 NH2 00G-4379-E0 00G-4379-N0 AJ0-4302 AJ0-7364
NH2 — 00D-4378-P0-EX 00F-4378-P0-AX 00G-4378-P0-AX — — — AJ0-8162 AJ0-8309 SCX 00G-4401-E0 00G-4401-N0 AJ0-4308 AJ0-7369
PFP(2) 00B-4448-P0-AX 00D-4448-P0-AX 00F-4448-P0-AX 00G-4448-P0-AX 00B-4448-U0-AX 00D-4448-U0-AX 00G-4448-U0-AX AJ0-8377 AJ0-8378 for ID: 3.2-8.0 mm 9-16 mm
HILIC 00B-4450-P0-AX 00D-4450-P0-AX 00F-4450-P0-AX 00G-4450-P0-AX — — 00G-4450-U0-AX –– ––
for ID: 18-29 mm 30-49 mm

Axia Packed Preparative Columns (mm) SecurityGuard Cartridges (mm)

50 x 21.2 100 x 21.2 150 x 21.2 250 x 21.2 50 x 30 100 x 30 250 x 30 50 x 50 100 x 50 250 x 50 15 x 21.2** 15 x 30 ◊
Phases ea ea
10 μm
Silica(2) — — — 00G-4091-P0-AX — — 00G-4091-U0-AX — — — AJ0-7229 AJ0-8312
C5 — 00D-4092-P0-AX — 00G-4092-P0-AX — — — — — 00G-4092-V0-AX — —
C8(2) 00B-4250-P0-AX — 00F-4250-P0-AX 00G-4250-P0-AX 00B-4250-U0-AX — — — — 00G-4250-V0-AX AJ0-7840 AJ0-8302
C18(2) 00B-4253-P0-AX 00D-4253-P0-AX 00F-4253-P0-AX 00G-4253-P0-AX 00B-4253-U0-AX 00D-4253-U0-AX 00G-4253-U0-AX 00B-4253-V0-AX 00D-4253-V0-AX 00G-4253-V0-AX AJ0-7839 AJ0-8301
CN — — — 00G-4300-P0-AX — — — — — — AJ0-8220 AJ0-8311
Phenyl-Hexyl — — — 00G-4285-P0-AX — — 00G-4285-U0-AX — — — AJ0-7841 AJ0-8303
NH2 — — — 00G-4379-P0-AX — — — — — — AJ0-8162 AJ0-8309
for ID: 18-29 mm 30-49 mm

*SecurityGuard Analytical Cartridges require holder, Part No.: KJ0-4282 **PREP SecurityGuard Cartridges require holder, Part No.: AJ0-8223
◊
‡
SemiPrep SecurityGuard Cartridges require holder, Part No.: AJ0-7220 PREP SecurityGuard Cartridges require holder, Part No.: AJ0-8277

30 Phenomenex l WEB: www.phenomenex.com

Phenex™ Syringe Filters
For Sample and Solvent Filtration Prior to Chromatography!
• Less system downtime

• More consistent, reproducible results

• Increased column lifetime

4 mm Diameter 15 mm Diameter 25 - 28 mm Diameter

for ≤ 2 mL sample volumes for 2 – 10 mL sample volumes for 10 – 100 mL sample volumes
Membrane Type/Size Part No. Unit Price Part No. Unit Price Part No. Unit Price
Phenex Offers:
0.20 µm » Broad chemical » 100 % integrity tested
Phenex-RC AF0-3203-12 100/pk AF0-2203-12 100/pk AF0-8203-12 5
100/pk compatibility
(Regenerated Cellulose) » Low hold-up volume
AF0-3203-52 500/pk AF0-2203-52 500/Pk AF0-8203-52 5
500/pk » Minimized extractables
» Low protein absorption
Phenex-PES 3 — — — — — — AF0-8208-12 7
100/pk
(Polyethersulfone) » Excellent flow rate
— — — — — — AF0-8208-52 7
500/pk » Bi-directional use
» High total throughput
Phenex-PTFE® 6 AF0-3202-12 100/pk AF0-2202-12 100/pk AF0-1202-12 100/pk
(Polytetrafluoroethylene) » Certified quality
AF0-3202-52 500/pk AF0-2202-52 500/pk AF0-1202-52 500/pk
Phenex-NY AF3-3207-12 100/pk AF0-2207-12 100/pk AF0-1207-12 100/pk
(Nylon) Tip: Try a Sample Pack!
AF3-3207-52 500/pk AF0-2207-52 500/pk AF0-1207-52 500/pk
Phenex-GF/NY 2 An integrated syringe filter unit containing an inert borosilicate glass fiber prefilter and a Nylon AF0-1A47-12 7 100/pk The best way to determine if a specific Phenex
(Glass Fiber/Nylon) (NY) membrane. Excellent for filtration of particle-laden samples, such as foods and beverages, membrane is suitable for your application.
environmental, biofuels, and dissolution samples. Use less hand pressure to filter even the most AF0-1A47-52 7
500/pk
Request yours today by phone or visit
difficult samples. Outlet connection is luer-lock. www.phenomenex.com/sample
Phenex-GF/CA 2,3,4
An integrated syringe filter unit containing an inert borosilicate glass fiber prefilter and a CA AF0-8A09-12 7
100/pk
(Glass Fiber/Cellulose Acetate) membrane. Excellent for filtration of tissue culture media, general biological sample filtration
and clarification. Outlet connection is luer lock. AF0-8A09-52 7
500/pk
Above syringe filters are non-sterile. Housing is made
0.45 µm of medical-grade polypropylene (PP), and offer luer lock
Phenex-RC AF0-3103-12 100/pk AF0-2103-12 100/pk AF0-8103-12 5
100/pk inlet/slip outlet connections, unless otherwise indicated.
(Regenerated Cellulose)
AF0-3103-52 500/pk AF0-2103-52 500/Pk AF0-8103-52 5
500/pk
1. Larger quantity purchases at significant savings are available.
Phenex-PES 3 — — — — — — AF0-8108-12 7
100/pk 2. Glass fiber filters are 28 mm diameter and made of borosilicate. If Phenex Syringe Filters do not
(Polyethersulfone) They will remove 90 % of all particles >1.2 µm. perform as well or better than your
— — — — — — AF0-8108-52 7
500/pk 3. Housing material is methacrylate butadiene styrene (MBS) current syringe filter product of
Phenex-PTFE 6 AF0-3102-12 100/pk AF0-2102-12 100/pk AF0-1102-12 100/pk polymerisate. similar membrane, diameter and
(Polytetrafluoroethylene) Also known as Cyrolite®. pore size, send in your comparative
AF0-3102-52 500/pk AF0-2102-52 500/pk AF0-1102-52 500/pk 4. Cellulose acetate is surfactant-free.
data within 45 days and keep the
5. 26 mm diameter.
Phenex-NY AF3-3107-12 100/pk AF0-2107-12 100/pk AF0-1107-12 100/pk 6. Hydrophobic membrane. Can be made hydrophilic by pre- Phenex product for FREE.
(Nylon) wetting with IPA.
AF3-3107-52 500/pk AF0-2107-52 500/pk AF0-1107-52 500/pk
7. 28 mm diameter.
Phenex-GF/NY 2 An integrated syringe filter unit containing an inert borosilicate glass fiber prefilter and a Nylon AF0-1B47-12 7 100/pk 8. Additional dimensions and membrane types are available.
(Glass Fiber/Nylon) (NY) membrane. Excellent for filtration of particle-laden samples, such as foods and beverages, Please contact your local Phenomenex technical consultant or
AF0-1B47-52 7 500/pk distributor for availability or assistance.
environmental, biofuels, and dissolution samples. Use less hand pressure to filter even the most
difficult samples. Outlet connection is luer-lock.
Terms and Conditions
Phenex-GF/CA 2,3,4 An integrated syringe filter unit containing an inert borosilicate glass fiber prefilter and a CA AF0-8B09-12 7
100/pk Subject to Phenomenex Standard Terms & Conditions, which may be viewed
(Glass Fiber/Cellulose Acetate) membrane. Excellent for filtration of tissue culture media, general biological sample filtration at www.phenomenex.com/TermsAndConditions
AF0-8B09-52 7
500/pk
and clarification. Outlet connection is luer lock. Trademarks
Luna, Gemini, and Synergi are registered trademarks of Phenomenex. Phenex, Axia, MercuryMS and
1.20 µm SecurityGuard are trademarks of Phenomenex. ZORBAX is a registered trademark of Agilent Technologies.
Phenex-GF 2,3 Prefiltration of heavily contaminated or highly viscous samples. When used in-series preced- AF0-8515-12 7
100/pk Symmetry, Spherisorb, Atlantis, and XTerra are registered trademarks of Waters Corp. OBD is a trademark of
(Glass Fiber) ing a membrane filter, clogging of the membrane filter is prevented and sample clean up is Waters Corp. Supelcosil is a trademark of Sigma-Aldrich Biotechnology. Nucleosil is a registered trademark
AF0-8515-52 7
500/pk of Macherey-Nagel. HyPURITY is a registered trademark of Thermo Hypersil-Keystone LLC. Cyrolite is a
optimized. Outlet connection is luer lock.
registered trademark of CY/RO Industries. ZIC is a registered trademark of Merck SeQuant AB.
Comparative separations may not be representative of all applications. Phenomenex is in no way affiliated with Agilent, Sigma-Aldrich Biotechnology, Waters Corp., Macherey-Nagel, Thermo, Merck or CY/RO Industries. Disclaimer
Axia and SecurityGuard are patented by Phenomenex.
© 2012 Phenomenex, Inc. All rights reserved.
 Australia Finland Italy Norway All other countries:
t: 02-9428-6444 t: 09 4789 0063 t: 051 6327511 t: 810 02 005 Corporate Office USA
f: 02-9428-6445 f: +45 4810 6265 f: 051 6327555 f: +45 4810 6265 t: (310) 212-0555
[email protected] [email protected] [email protected] [email protected] f: (310) 328-7768
[email protected]
Austria France Luxembourg Puerto Rico
t: 01-319-1301 t: 01 30 09 21 10 t: +31 (0)30-2418700 t: (800) 541-HPLC
f: 01-319-1300 f: 01 30 09 21 11 f: +31 (0)30-2383749 f: (310) 328-7768
[email protected] [email protected] [email protected] [email protected]

Belgium Germany Mexico Sweden

t: 02 503 4015 (French) t: 06021-58830-0 t: 001-800-844-5226 t: 08 611 6950
t: 02 511 8666 (Dutch) f: 06021-58830-11 f: 001-310-328-7768 f: +45 4810 6265
f: +31 (0)30-2383749 [email protected] [email protected] [email protected]
[email protected] India The Netherlands United Kingdom
Canada t: 040-3012 2400 t: 030-2418700 t: 01625-501367
t: (800) 543-3681 f: 040-3012 2411 f: 030-2383749 f: 01625-501796
f: (310) 328-7768 [email protected] [email protected] [email protected]
[email protected] Ireland New Zealand United States
Denmark t: 01 247 5405 t: 09-4780951 t: (310) 212-0555
t: 4824 8048 f: +44 1625-501796 f: 09-4780952 f: (310) 328-7768
f: +45 4810 6265 [email protected] [email protected] [email protected]
[email protected]

BR83200612_W
www.phenomenex.com
Phenomenex products are available worldwide. For the distributor in your country,
contact Phenomenex USA, International Department at [email protected]