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Laboratory Exercise Methods

The document describes a laboratory exercise on cellular respiration. It outlines two methodologies - the first uses beans and calcium hydroxide in test tubes to observe differences in water height, the second uses phenol red indicator and volunteers' exhaled breath before and after exercise to observe changes in color. The document provides details on the setup, materials, and measurements for each methodology.

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33 views

Laboratory Exercise Methods

The document describes a laboratory exercise on cellular respiration. It outlines two methodologies - the first uses beans and calcium hydroxide in test tubes to observe differences in water height, the second uses phenol red indicator and volunteers' exhaled breath before and after exercise to observe changes in color. The document provides details on the setup, materials, and measurements for each methodology.

Uploaded by

Jane
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
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Laboratory Exercise No.

TITLE: CELLULAR RESPIRATION

COURSE TITLE : Anatomy and Physiology

SECTION : EFGSE

LAB INSTRUCTOR/PROFESSOR : Professor Ronald Bigsang, M.Sc.

DATE PPERFORMED : August 11, 2014

DATE SUBMITTED : August 15, 2014

GROUP No. : Group 6

MEMBERS:

LARIDA, JANE A.
TAMAMA, SITTI ALYSSA
CADATUAN, RIZALYN
LUMAPAT, DARLENE
METHODOLOGY

I. Using Beans
In this activity seven test tubes were used, three test tubes were labelled
with treatment 1, another three test tubes for treatment 2 and one test tube for
the control. Treatment 1 set-up should have the following order 1.5 grams of
calcium hydroxide at the bottom, cotton balls, at least three germinating
seeds and another cotton ball. For treatment 2, set up are still the same but
instead of germinating seeds you will put dry beans. For the control test tube
will only have 1.5 grams of calcium hydroxide and a cotton ball. Pour 50 mL
of water on seven 100 mL beaker. Place each test tube upside down in the
beaker and record the height that water rises in each of the beaker. After 2
hours measure and record the changes in height of the water.

t
re
atment
1 treatment 2 control

II. Using Exhaled Breath


Put 20 mL of phenol red in three Erlenmeyer flask. Ask three
volunteers to blow the phenol red indicator as fast as they can. Record the
time that the phenol red turns to orange in color of each volunteers. Let
the volunteers take a 3 minute exercise after that let them blow again the
phenol red using the straw. Record and compare how fast each volunteers
change the phenol red to orange by just blowing it with straw.

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