8/6/23, 12:22 PM Spatial Transcriptomics - 10x Genomics

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Spatially resolved biology

Novel insights about

your tissue. Visualized.

Uncover the
"Where" for every
"What"
The relationship between cells and their relative locations
within tissue is critical to understanding normal
development and disease pathology. Spatial
transcriptomics is a groundbreaking molecular profiling
method that allows scientists to measure all the gene
activity in a tissue sample and map where the activity is
occurring. Already this technology is leading to new
discoveries that are proving instrumental in helping

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8/6/23, 12:22 PM Spatial Transcriptomics - 10x Genomics

scientists gain a better understanding of biological

processes and disease.

Download Technology Brochure

How it works

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8/6/23, 12:22 PM Spatial Transcriptomics - 10x Genomics

Fuel your spatial

discoveries with
spatial capture
technology
Spatial capture technology powers the Visium Spatial
platform through the use of spatially barcoded mRNA-
binding oligonucleotides. There are two methods for how
mRNA molecules get a spatial barcode.

In fresh frozen tissues, the tissue is fixed and permeabilized

to release RNA which binds to adjacent capture probes,
allowing for the capture of gene expression information.
cDNA is then synthesized from captured RNA and
sequencing libraries prepared.

In FFPE tissues, the tissue is permeabilized to release

ligated probe pairs that bind to adjacent capture probes on
the slide, allowing for the capture of gene expression
information. Pairs of probes specific to each gene in the
protein-coding transcriptome are hybridized to their gene
target and then ligated to one another. The probe pairs are
extended to incorporate complements of the spatial
barcodes and sequencing libraries prepared.

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Seminars

Examine gene
expression in the
context of the tissue
microenvironment
Innovation in spatial transcriptomics methodologies is
enabling scientists to get a holistic understanding of cells
in their morphological context. In this presentation, you will
hear first-hand from 10x Genomics scientists about
groundbreaking improvements to the technology and
exciting applications showcased by users.

Publications

Versican promotes T helper 17

Gain a holistic cytotoxic inflammation and impedes
understanding of gene oligodendrocyte precursor cell
remyelination
and protein expression Ghorbani S, et al. Nature Communications 2022.

in the tissue
microenvironment
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8/6/23, 12:22 PM Spatial Transcriptomics - 10x Genomics

See how researchers are using spatial transcriptomics to

elevate the understanding of complex transcriptional
landscapes within a spatial context, which is essential for the Single-cell and spatial analysis reveal
understanding of biology and complex disease. interaction of FAP+ fibroblasts and
SPP1+ macrophages in colorectal
cancer
Qi J, et al. Nature Communications 2022.

Spatial transcriptomics of dorsal root

ganglia identifies molecular
signatures of human nociceptors
Tavares-Ferreira D, et al. Science Translational Medicine
2022.

View all Visium Spatial publications

Explore data The data generated by spatial capture technology allows you to choose any
gene of interest and display its spatially resolved expression on the original

visualization tissue section. Since the whole transcriptome is measured, you are not
limited to visualizing only a single gene. You can choose any number of
genes in any combination to view and analyze together.

Play with the data visualization and table below

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Visualization Controls Spatial Image t-SNE

Use the sliders under the tissue image Opacity

to adjust how you visualize and

combine the tissue image and the +
gene expression data. Colors −
represent clusters identified by
differentially expressed genes.

Leaflet

Cluster 1 2 3 4 5 6 7 8 9
Gene Identification
Nptxr 3.5 1.1 2.2 4.7 1.8 4.1 1.6 1.2 3.1
By placing the pointer above a gene Agt 0.59 2.0 3.6 1.2 0.78 0.42 3.2 2.6 0.61
name within the table, spots in the Ttr 3.5 4.9 3.0 4.8 3.1 2.6 2.7 2.8 3.8
tissue image will be colored based on
Pmch 0.77 1.8 4.5 1.5 0.56 0.79 1.3 0.63 0.66
the expression of that gene.
Camk2n1 5.7 3.7 3.9 5.8 3.9 6.8 4.3 4.2 5.1
Alternatively, by placing the pointer
above a value within the table, you Olfm1 5.6 2.8 3.5 5.9 3.1 5.5 3.2 3.6 4.5

can observe the expression of a Pcp4 4.9 2.8 4.2 4.4 2.0 2.5 3.3 6.2 3.0
specific gene with the spots from an Prkcd 0.50 0.81 0.72 0.51 0.48 0.31 1.6 4.7 0.43
individual cluster highlighted. Cck 5.5 2.4 2.3 4.8 3.0 5.2 2.3 4.4 4.2
Nnat 2.2 2.7 5.2 3.8 1.6 1.2 3.0 1.8 2.4

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Beyond gene expression Turnkey solution Easily adoptable workflow Streamlined data analysis

Layer transcriptome and proteome Compatible with diverse sample Integrate easily with your current Analyze and understand gene and
data onto your stained images to types across preparation (FFPE laboratory methods and tools for protein expression heterogeneity
understand tissue and fresh frozen) and species tissue analysis. Combine with with Space Ranger analysis
microenvironments like never (human, mouse, rat, and more), immunofluorescence staining and software to process your data, and
before. Reveal the spatial easily incorporate into your imaging to gain multiomic interactively explore the results
organization of newly discovered research with all the reagents characterization in the spatial with Loupe Browser visualization
cell types, states, and biomarkers. kitted and ready to use and no context. software.
specialized instrumentation
required.

Now available for order

View Spatial Products View Pricing

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