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1 - Reagent Parameter Settings

This document provides instructions and parameters for testing multiple items including ALT, AST, CHO, CRE, GGT, GLU, HDL-C, LDL-C, TG, UA, and UREA using a chemistry analyzer. It lists the test method, wavelengths, volumes, times, units, temperature and calibration for each item and provides the procedure for testing each which involves pipetting reagents and sample and incubating before measurement.
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0% found this document useful (0 votes)
19 views

1 - Reagent Parameter Settings

This document provides instructions and parameters for testing multiple items including ALT, AST, CHO, CRE, GGT, GLU, HDL-C, LDL-C, TG, UA, and UREA using a chemistry analyzer. It lists the test method, wavelengths, volumes, times, units, temperature and calibration for each item and provides the procedure for testing each which involves pipetting reagents and sample and incubating before measurement.
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
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Reagent parameter settings

Test item ALT

Method Rate Method


Wave1 340nm
Wave2 /
Sample vol(ul) 500
Hatch time(s) 60
Test time(s) 250
Unit U/L
Decimal 1
Temp 37℃
Linear 10.0~1000.0
Calibration Type Linear - 2P

1.Pipette 600µL of R1 and 30 µL of sample into a


test tube.Mix and after 300 secondes incubation at 37°C.
PROCEDURE:
2.Pipette 150µL of R2 into the test tube.Mix and aspirate the
mixing by the analyser and start measurement.

Test item AST

Method Rate Method


Wave1 340nm
Wave2 /
Sample vol(ul) 500
Hatch time(s) 60
Test time(s) 250
Unit mg/dl
Decimal 1
Temp 37℃
Linear 10.0~900.0
Calibration Type Linear - 2P

1.Pipette 600µL of R1 and 30 µL of sample into a


test tube.Mix and after 300 secondes incubation at 37°C.
PROCEDURE:
2.Pipette 150µL of R2 into the test tube.Mix and aspirate the
mixing by the analyser and start measurement.
Test item CHO

Method End point Method


Wave1 510
Wave2 /
Sample vol(ul) 500
Hatch time(s) 5
Test time(s) 5
Unit mmol/L
Decimal 1
Temp 37℃
Linear 0.3~19.4
Calibration Type Linear - 2P

1.Pipette 1000µL of R1 and 10 µL of sample into a


PROCEDURE: test tube.Mix and after 600 secondes incubation at 37°C.
aspirate the mixing by the analyser and start measurement.

Test item CRE

Method End point Method


Wave1 546
Wave2 /
Sample vol(ul) 500
Hatch time(s) 5
Test time(s) 5
Unit μmol/L
Decimal 0
Temp 37℃
Linear 30~1500
Calibration Type Linear - 2P

1.Pipette 667µL of R1,333µL of R2 and 10 µL of sample into a


PROCEDURE: test tube.Mix and after 300 secondes incubation at 37°C.
aspirate the mixing by the analyser and start measurement.
Test item GGT

Method Rate Method


Wave1 405
Wave2 /
Sample vol(ul) 500
Hatch time(s) 90
Test time(s) 210
Unit U/L
Decimal 0
Temp 37℃
Linear 10~1200
Calibration Type Linear - 2P

1.Pipette 576µL of R1 and 24 µL of sample into a


test tube.Mix and after 300 secondes incubation at 37°C.
PROCEDURE:
2.Pipette 192µL of R2 into the test tube.Mix and aspirate the
mixing by the analyser and start measurement.

Test item GLU

Method End point Method


Wave1 510
Wave2 /
Sample vol(ul) 500
Hatch time(s) 5
Test time(s) 5
Unit mmol/L
Decimal 1
Temp 37℃
Linear 2.2~25.0
Calibration Type Linear - 2P

1.Pipette 1000µL of R1 and 10 µL of sample into a


PROCEDURE: test tube.Mix and after 600 secondes incubation at 37°C.
aspirate the mixing by the analyser and start measurement.
Test item HDL-C

Method End point Method


Wave1 578
Wave2 /
Sample vol(ul) 500
Hatch time(s) 5
Test time(s) 5
Unit μmol/L
Decimal 2
Temp 37℃
Linear 0.20~2,58
Calibration Type Linear - 2P

1.Pipette 667µL of R1,333µL of R2 and 20 µL of sample into a


PROCEDURE: test tube.Mix and after 300 secondes incubation at 37°C.
aspirate the mixing by the analyser and start measurement.

Test item LDL-C

Method End point Method


Wave1 578
Wave2 /
Sample vol(ul) 500
Hatch time(s) 5
Test time(s) 5
Unit mmol/L
Decimal 1
Temp 37℃
Linear 0.3~12.0
Calibration Type Linear - 2P

1.Pipette 667µL of R1,333µL of R2 and 20 µL of sample into a


PROCEDURE: test tube.Mix and after 300 secondes incubation at 37°C.
aspirate the mixing by the analyser and start measurement.
Test item TG

Method End point Method


Wave1 510
Wave2 /
Sample vol(ul) 500
Hatch time(s) 5
Test time(s) 5
Unit mmol/L
Decimal 1
Temp 37℃
Linear 0.3~11.4
Calibration Type Linear - 2P

1.Pipette 1000µL of R1 and 10 µL of sample into a


PROCEDURE: test tube.Mix and after 600 secondes incubation at 37°C.
aspirate the mixing by the analyser and start measurement.

Test item UA

Method End point Method


Wave1 546
Wave2 /
Sample vol(ul) 500
Hatch time(s) 5
Test time(s) 5
Unit μmol/L
Decimal 1
Temp 37℃
Linear 29.0~1190.0
Calibration Type Linear - 2P

1.Pipette 800µL of R1,200µL of R2 and 20 µL of sample into a


PROCEDURE: test tube.Mix and after 300 secondes incubation at 37°C.
aspirate the mixing by the analyser and start measurement.
Test item UREA

Method Rate Method


Wave1 340nm
Wave2 /
Sample vol(ul) 500
Hatch time(s) 60
Test time(s) 250
Unit mmol/L
Decimal 2
Temp 37℃
Linear 0.17~44.00
Calibration Type Linear - 2P

1.Pipette 600µL of R1 and 8 µL of sample into a


test tube.Mix and after 300 secondes incubation at 37°C.
PROCEDURE:
2.Pipette 200µL of R2 into the test tube.Mix and aspirate the
mixing by the analyser and start measurement.

Test item Albumin

Method End point Method


Wave1 578
Wave2 /
Sample vol(ul) 500
Hatch time(s) 5
Test time(s) 5
Unit g/L
Decimal 1
Temp 37℃
Linear 2.0~60.0
Calibration Type Linear - 2P

1.Pipette 1000µL of R1 and 10 µL of sample into a test


PROCEDURE: tube.Mix and after 180 secondes incubation at 37°C. aspirate
the mixing by the analyser and start measurement.
Test item Alkali phosphatase

Method Rate Method


Wave1 405
Wave2 /
Sample vol(ul) 500
Hatch time(s) 90
Test time(s) 150
Unit U/L
Decimal 0
Temp 37℃
Linear 3~1702
Calibration Type Linear - 2P

1.Pipette 800µL of R1,200µL of R2 and 20 µL of sample into a


PROCEDURE: test tube.Mix and aspirate the mixing by the analyser and
start measurement.

Test item Direct bilirubin

Method End point Method


Wave1 546
Wave2 /
Sample vol(ul) 500
Hatch time(s) 5
Test time(s) 5
Unit μmol/L
Decimal 1
Temp 37℃
Linear 2.5~171.0
Calibration Type Linear - 2P

1.Pipette 800µL of R1,200µL of R2 and 20 µL of sample into a


PROCEDURE: test tube.Mix and after 300 secondes incubation at 37°C.
aspirate the mixing by the analyser and start measurement.
Test item Total Protein

Method End point Method


Wave1 546
Wave2 /
Sample vol(ul) 500
Hatch time(s) 5
Test time(s) 5
Unit g/L
Decimal 1
Temp 37℃
Linear 2.0~120.0
Calibration Type Linear - 2P

1.Pipette 800µL of R1 and 16 µL of sample into a


PROCEDURE: test tube.Mix and after 600 secondes incubation at 37°C.
aspirate the mixing by the analyser and start measurement.

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