This document provides instructions and parameters for testing multiple items including ALT, AST, CHO, CRE, GGT, GLU, HDL-C, LDL-C, TG, UA, and UREA using a chemistry analyzer. It lists the test method, wavelengths, volumes, times, units, temperature and calibration for each item and provides the procedure for testing each which involves pipetting reagents and sample and incubating before measurement.
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1 - Reagent Parameter Settings
This document provides instructions and parameters for testing multiple items including ALT, AST, CHO, CRE, GGT, GLU, HDL-C, LDL-C, TG, UA, and UREA using a chemistry analyzer. It lists the test method, wavelengths, volumes, times, units, temperature and calibration for each item and provides the procedure for testing each which involves pipetting reagents and sample and incubating before measurement.
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Reagent parameter settings
Test item ALT
Method Rate Method
Wave1 340nm Wave2 / Sample vol(ul) 500 Hatch time(s) 60 Test time(s) 250 Unit U/L Decimal 1 Temp 37℃ Linear 10.0~1000.0 Calibration Type Linear - 2P
1.Pipette 600µL of R1 and 30 µL of sample into a
test tube.Mix and after 300 secondes incubation at 37°C. PROCEDURE: 2.Pipette 150µL of R2 into the test tube.Mix and aspirate the mixing by the analyser and start measurement.
Test item AST
Method Rate Method
Wave1 340nm Wave2 / Sample vol(ul) 500 Hatch time(s) 60 Test time(s) 250 Unit mg/dl Decimal 1 Temp 37℃ Linear 10.0~900.0 Calibration Type Linear - 2P
1.Pipette 600µL of R1 and 30 µL of sample into a
test tube.Mix and after 300 secondes incubation at 37°C. PROCEDURE: 2.Pipette 150µL of R2 into the test tube.Mix and aspirate the mixing by the analyser and start measurement. Test item CHO
Method End point Method
Wave1 510 Wave2 / Sample vol(ul) 500 Hatch time(s) 5 Test time(s) 5 Unit mmol/L Decimal 1 Temp 37℃ Linear 0.3~19.4 Calibration Type Linear - 2P
1.Pipette 1000µL of R1 and 10 µL of sample into a
PROCEDURE: test tube.Mix and after 600 secondes incubation at 37°C. aspirate the mixing by the analyser and start measurement.
Test item CRE
Method End point Method
Wave1 546 Wave2 / Sample vol(ul) 500 Hatch time(s) 5 Test time(s) 5 Unit μmol/L Decimal 0 Temp 37℃ Linear 30~1500 Calibration Type Linear - 2P
1.Pipette 667µL of R1,333µL of R2 and 10 µL of sample into a
PROCEDURE: test tube.Mix and after 300 secondes incubation at 37°C. aspirate the mixing by the analyser and start measurement. Test item GGT
Method Rate Method
Wave1 405 Wave2 / Sample vol(ul) 500 Hatch time(s) 90 Test time(s) 210 Unit U/L Decimal 0 Temp 37℃ Linear 10~1200 Calibration Type Linear - 2P
1.Pipette 576µL of R1 and 24 µL of sample into a
test tube.Mix and after 300 secondes incubation at 37°C. PROCEDURE: 2.Pipette 192µL of R2 into the test tube.Mix and aspirate the mixing by the analyser and start measurement.
Test item GLU
Method End point Method
Wave1 510 Wave2 / Sample vol(ul) 500 Hatch time(s) 5 Test time(s) 5 Unit mmol/L Decimal 1 Temp 37℃ Linear 2.2~25.0 Calibration Type Linear - 2P
1.Pipette 1000µL of R1 and 10 µL of sample into a
PROCEDURE: test tube.Mix and after 600 secondes incubation at 37°C. aspirate the mixing by the analyser and start measurement. Test item HDL-C
Method End point Method
Wave1 578 Wave2 / Sample vol(ul) 500 Hatch time(s) 5 Test time(s) 5 Unit μmol/L Decimal 2 Temp 37℃ Linear 0.20~2,58 Calibration Type Linear - 2P
1.Pipette 667µL of R1,333µL of R2 and 20 µL of sample into a
PROCEDURE: test tube.Mix and after 300 secondes incubation at 37°C. aspirate the mixing by the analyser and start measurement.
Test item LDL-C
Method End point Method
Wave1 578 Wave2 / Sample vol(ul) 500 Hatch time(s) 5 Test time(s) 5 Unit mmol/L Decimal 1 Temp 37℃ Linear 0.3~12.0 Calibration Type Linear - 2P
1.Pipette 667µL of R1,333µL of R2 and 20 µL of sample into a
PROCEDURE: test tube.Mix and after 300 secondes incubation at 37°C. aspirate the mixing by the analyser and start measurement. Test item TG
Method End point Method
Wave1 510 Wave2 / Sample vol(ul) 500 Hatch time(s) 5 Test time(s) 5 Unit mmol/L Decimal 1 Temp 37℃ Linear 0.3~11.4 Calibration Type Linear - 2P
1.Pipette 1000µL of R1 and 10 µL of sample into a
PROCEDURE: test tube.Mix and after 600 secondes incubation at 37°C. aspirate the mixing by the analyser and start measurement.
Test item UA
Method End point Method
Wave1 546 Wave2 / Sample vol(ul) 500 Hatch time(s) 5 Test time(s) 5 Unit μmol/L Decimal 1 Temp 37℃ Linear 29.0~1190.0 Calibration Type Linear - 2P
1.Pipette 800µL of R1,200µL of R2 and 20 µL of sample into a
PROCEDURE: test tube.Mix and after 300 secondes incubation at 37°C. aspirate the mixing by the analyser and start measurement. Test item UREA
Method Rate Method
Wave1 340nm Wave2 / Sample vol(ul) 500 Hatch time(s) 60 Test time(s) 250 Unit mmol/L Decimal 2 Temp 37℃ Linear 0.17~44.00 Calibration Type Linear - 2P
1.Pipette 600µL of R1 and 8 µL of sample into a
test tube.Mix and after 300 secondes incubation at 37°C. PROCEDURE: 2.Pipette 200µL of R2 into the test tube.Mix and aspirate the mixing by the analyser and start measurement.
Test item Albumin
Method End point Method
Wave1 578 Wave2 / Sample vol(ul) 500 Hatch time(s) 5 Test time(s) 5 Unit g/L Decimal 1 Temp 37℃ Linear 2.0~60.0 Calibration Type Linear - 2P
1.Pipette 1000µL of R1 and 10 µL of sample into a test
PROCEDURE: tube.Mix and after 180 secondes incubation at 37°C. aspirate the mixing by the analyser and start measurement. Test item Alkali phosphatase
Method Rate Method
Wave1 405 Wave2 / Sample vol(ul) 500 Hatch time(s) 90 Test time(s) 150 Unit U/L Decimal 0 Temp 37℃ Linear 3~1702 Calibration Type Linear - 2P
1.Pipette 800µL of R1,200µL of R2 and 20 µL of sample into a
PROCEDURE: test tube.Mix and aspirate the mixing by the analyser and start measurement.
Test item Direct bilirubin
Method End point Method
Wave1 546 Wave2 / Sample vol(ul) 500 Hatch time(s) 5 Test time(s) 5 Unit μmol/L Decimal 1 Temp 37℃ Linear 2.5~171.0 Calibration Type Linear - 2P
1.Pipette 800µL of R1,200µL of R2 and 20 µL of sample into a
PROCEDURE: test tube.Mix and after 300 secondes incubation at 37°C. aspirate the mixing by the analyser and start measurement. Test item Total Protein
Method End point Method
Wave1 546 Wave2 / Sample vol(ul) 500 Hatch time(s) 5 Test time(s) 5 Unit g/L Decimal 1 Temp 37℃ Linear 2.0~120.0 Calibration Type Linear - 2P
1.Pipette 800µL of R1 and 16 µL of sample into a
PROCEDURE: test tube.Mix and after 600 secondes incubation at 37°C. aspirate the mixing by the analyser and start measurement.