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Histopath Lab Activity

There are four main methods for examining fresh tissue samples microscopically: 1) Teasing or dissociation, where tissue is immersed and dissected in isotonic solution to preserve cell structure. 2) Squash preparation, where tissue fragments are crushed between slides. 3) Smear preparation, where tissue is applied to a slide in streaks, spreads, or touch preparations to view cells. 4) Frozen section, where very thin fresh tissue slices are cut in a cryostat, stained, and viewed immediately.

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0% found this document useful (0 votes)
623 views5 pages

Histopath Lab Activity

There are four main methods for examining fresh tissue samples microscopically: 1) Teasing or dissociation, where tissue is immersed and dissected in isotonic solution to preserve cell structure. 2) Squash preparation, where tissue fragments are crushed between slides. 3) Smear preparation, where tissue is applied to a slide in streaks, spreads, or touch preparations to view cells. 4) Frozen section, where very thin fresh tissue slices are cut in a cryostat, stained, and viewed immediately.

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Rakia Pillay
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ILLUSTRATE THE DIFFERENT METHODS OF FRESH TISSUE EXAMINATION.

1. TEASING OR DISSOCIATION

2. SQUASH PREPARATION (CRUSHING)


3. SMEARING PREPARATION

a. STREAKING

b. SPREADING
c. PULL APART

d. TOUCH PREPARATION (IMPRESSION SMEAR)

4. FROZEN SECTION
Methods of Fresh Tissue Examination

1. Teasing or Dissociation

- a tissue specimen is immersed in a watch glass containing isotonic solution and then
meticulously dissected or divided and studied under the microscope. The fluid medium
used usually has the property of dissolving the intracellular substance at the same time
fixes and preserves the cell structure. (Ex: Muller’s fluid, 1% Normal saline, 30% Alcohol)

2. Squash preparation (Crushing)

- small fragments of tissue are put on a microscopic slide and pushed down with the help of
another slide and a coverslip.’ If necessary, a vital stain can be applied to the junction of
the slide and the cover glass and allowed to diffuse via capillary attraction into the tissue.

3. Smear preparation

- A slide is delicately covered with cellular materials.

A. Streaking- The material is applied quickly yet gently with an applicator stick or
platinum loops in a straight or zigzag line throughout the slide, striving to achieve a
generally equal distribution of secrtion.

B. Spreading- A part of the material is transferred to a clean slide for analysis.


Spread thinly into a thick film.

C. Pull–apart- On two slide surfaces, thick secretions (gastric lavage, serum fluids,
blood) are equally disseminated.

D. Touch Preparation (Impression)- A newly sliced tissue surface is brought into

touch with the slide. Cells are viewed in their true intercellular interaction. It also
offers the benefit of allowing the cells to be inspected without disrupting their
natural intercellular interaction or isolating them from their surroundings.
4. Frozen Section

- for fast detection and display of lipids and nervous tissue components during surgery.
Using a microtome with CO2 or a Cyrostat, a cold chamber kept at an ambient temperature
of –10oC to–20oC, very thin slices of fresh tissue are cut, transferred to a plate
containing isotonic salt solution, and stained for microscopic study.

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