Histopath Lab Activity
Histopath Lab Activity
1. TEASING OR DISSOCIATION
a. STREAKING
b. SPREADING
c. PULL APART
4. FROZEN SECTION
Methods of Fresh Tissue Examination
1. Teasing or Dissociation
- a tissue specimen is immersed in a watch glass containing isotonic solution and then
meticulously dissected or divided and studied under the microscope. The fluid medium
used usually has the property of dissolving the intracellular substance at the same time
fixes and preserves the cell structure. (Ex: Muller’s fluid, 1% Normal saline, 30% Alcohol)
- small fragments of tissue are put on a microscopic slide and pushed down with the help of
another slide and a coverslip.’ If necessary, a vital stain can be applied to the junction of
the slide and the cover glass and allowed to diffuse via capillary attraction into the tissue.
3. Smear preparation
A. Streaking- The material is applied quickly yet gently with an applicator stick or
platinum loops in a straight or zigzag line throughout the slide, striving to achieve a
generally equal distribution of secrtion.
C. Pull–apart- On two slide surfaces, thick secretions (gastric lavage, serum fluids,
blood) are equally disseminated.
touch with the slide. Cells are viewed in their true intercellular interaction. It also
offers the benefit of allowing the cells to be inspected without disrupting their
natural intercellular interaction or isolating them from their surroundings.
4. Frozen Section
- for fast detection and display of lipids and nervous tissue components during surgery.
Using a microtome with CO2 or a Cyrostat, a cold chamber kept at an ambient temperature
of –10oC to–20oC, very thin slices of fresh tissue are cut, transferred to a plate
containing isotonic salt solution, and stained for microscopic study.