“PEARL”

NWR'S SALTWATER PEARL

DNA Test Report Test Date: March 16th, 2023 embk.me/nwrssaltwaterpearls

BREED ANCESTRY GENETIC STATS

Doberman Pinscher : 100.0% Predicted adult weight: 69 lbs

TEST DETAILS
Kit number: EM-17321604
Swab number: 31220511502239

Registration: American Kennel Club

(AKC)
 “PEARL”
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DNA Test Report Test Date: March 16th, 2023 embk.me/nwrssaltwaterpearls

DOBERMAN PINSCHER
The Doberman Pinscher is a relatively new breed, bred around 1890 by Karl Friedrich
Louis Doberman, a German tax collector. He aimed to breed a dog that would protect
him during his tax collections. Doberman Pinschers are intelligent, loyal, and make for
perfect companions as well as guard dogs. The Doberman is a mixture of many
different dog breeds that includes Beauceron, German Pinscher, German Shepherd,
and Rottweiler. The Doberman is a very athletic dog that often excels in agility
courses. Doberman’s are trainable and are listed as one of the top five smartest dogs.

Fun Fact
A Doberman named Cappy saved the
lives of 250 U.S. Marines on Guam in
1944 by alerting them when Japanese
troops were nearby.

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DNA Test Report Test Date: March 16th, 2023 embk.me/nwrssaltwaterpearls

MATERNAL LINE

Through Pearl’s mitochondrial DNA we can trace her mother’s ancestry back to where dogs and people first became friends. This
map helps you visualize the routes that her ancestors took to your home. Their story is described below the map.

HAPLOGROUP: B1 HAPLOTYPE: B95

B1 is the second most common maternal lineage in breeds of Part of the B1 haplogroup, we see this haplotype most
European or American origin. It is the female line of the frequently in mixed breed dogs.
majority of Golden Retrievers, Basset Hounds, and Shih Tzus,
and about half of Beagles, Pekingese and Toy Poodles. This
lineage is also somewhat common among village dogs that
carry distinct ancestry from these breeds. We know this is a
result of B1 dogs being common amongst the European dogs
that their conquering owners brought around the world,
because nowhere on earth is it a very common lineage in
village dogs. It even enables us to trace the path of (human)
colonization: Because most Bichons are B1 and Bichons are
popular in Spanish culture, B1 is now fairly common among
village dogs in Latin America.

Registration: American Kennel Club

(AKC)
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DNA Test Report Test Date: March 16th, 2023 embk.me/nwrssaltwaterpearls

TRAITS: COAT COLOR

TRAIT RESULT

E Locus (MC1R)

The E Locus determines if and where a dog can produce dark (black or brown) hair. Dogs with two copies
of the recessive e allele do not produce dark hairs at all, and will be “red” over their entire body. The shade
of red, which can range from a deep copper to yellow/gold to cream, is dependent on other genetic factors
Can have a melanistic
including the Intensity loci. In addition to determining if a dog can develop dark hairs at all, the E Locus can
mask (EmEm)
give a dog a black “mask” or “widow’s peak,” unless the dog has overriding coat color genetic factors.
Dogs with one or two copies of the Em allele usually have a melanistic mask (dark facial hair as commonly
seen in the German Shepherd and Pug). Dogs with no copies of Em but one or two copies of the Eg allele
usually have a melanistic "widow's peak" (dark forehead hair as commonly seen in the Afghan Hound and
Borzoi, where it is called either “grizzle” or “domino”).

K Locus (CBD103)

The K Locus KB allele “overrides” the A Locus, meaning that it prevents the A Locus genotype from
affecting coat color. For this reason, the KB allele is referred to as the “dominant black” allele. As a result, More likely to have a
dogs with at least one KB allele will usually have solid black or brown coats (or red/cream coats if they are patterned haircoat
ee at the E Locus) regardless of their genotype at the A Locus, although several other genes could impact (kyky)
the dog’s coat and cause other patterns, such as white spotting. Dogs with the kyky genotype will show a
coat color pattern based on the genotype they have at the A Locus. Dogs who test as KBky may be brindle
rather than black or brown.

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TRAITS: COAT COLOR (CONTINUED)

TRAIT RESULT

Intensity Loci LINKAGE

Areas of a dog's coat where dark (black or brown) pigment is not expressed either contain red/yellow
pigment, or no pigment at all. Five locations across five chromosomes explain approximately 70% of red Any light hair likely
pigmentation "intensity" variation across all dogs. Dogs with a result of Intense Red Pigmentation will likely yellow or tan
have deep red hair like an Irish Setter or "apricot" hair like some Poodles, dogs with a result of (Intermediate Red
Intermediate Red Pigmentation will likely have tan or yellow hair like a Soft-Coated Wheaten Terrier, and Pigmentation)
dogs with Dilute Red Pigmentation will likely have cream or white hair like a Samoyed. Because the
mutations we test may not directly cause differences in red pigmentation intensity, we consider this to be
a linkage test.

A Locus (ASIP)

The A Locus controls switching between black and red pigment in hair cells, but it will only be expressed
Black/Brown and tan
in dogs that are not ee at the E Locus and are kyky at the K Locus. Sable (also called “Fawn”) dogs have a
coat color pattern
mostly or entirely red coat with some interspersed black hairs. Agouti (also called “Wolf Sable”) dogs have
(atat)
red hairs with black tips, mostly on their head and back. Black and tan dogs are mostly black or brown with
lighter patches on their cheeks, eyebrows, chest, and legs. Recessive black dogs have solid-colored black
or brown coats.

D Locus (MLPH)

The D locus result that we report is determined by two different genetic variants that can work together to
cause diluted pigmentation. These are the common d allele, also known as “d1”, and a less common allele
known as “d2”. Dogs with two d alleles, regardless of which variant, will have all black pigment lightened
Dark areas of hair and
(“diluted”) to gray, or brown pigment lightened to lighter brown in their hair, skin, and sometimes eyes.
skin are lightened (dd)
There are many breed-specific names for these dilute colors, such as “blue”, “charcoal”, “fawn”, “silver”,
and “Isabella”. Note that in certain breeds, dilute dogs have a higher incidence of Color Dilution Alopecia.
Dogs with one d allele will not be dilute, but can pass the d allele on to their puppies. To view your dog’s d1
and d2 test results, click the “SEE DETAILS” link in the upper right hand corner of the “Base Coat Color”
section of the Traits page, and then click the “VIEW SUBLOCUS RESULTS” link at the bottom of the page.

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TRAITS: COAT COLOR (CONTINUED)

TRAIT RESULT

Cocoa (HPS3)

Dogs with the coco genotype will produce dark brown pigment instead of black in both their hair and skin. No co alleles, not
Dogs with the Nco genotype will produce black pigment, but can pass the co allele on to their puppies. expressed (NN)
Dogs that have the coco genotype as well as the bb genotype at the B locus are generally a lighter brown
than dogs that have the Bb or BB genotypes at the B locus.

B Locus (TYRP1)

Dogs with two copies of the b allele produce brown pigment instead of black in both their hair and skin.
Brown hair and skin
Dogs with one copy of the b allele will produce black pigment, but can pass the b allele on to their puppies.
(bb)
E Locus ee dogs that carry two b alleles will have red or cream coats, but have brown noses, eye rims, and
footpads (sometimes referred to as "Dudley Nose" in Labrador Retrievers). “Liver” or “chocolate” is the
preferred color term for brown in most breeds; in the Doberman Pinscher it is referred to as “red”.

Saddle Tan (RALY)

The "Saddle Tan" pattern causes the black hairs to recede into a "saddle" shape on the back, leaving a tan
face, legs, and belly, as a dog ages. The Saddle Tan pattern is characteristic of breeds like the Corgi, Not saddle tan
Beagle, and German Shepherd. Dogs that have the II genotype at this locus are more likely to be mostly patterned (II)
black with tan points on the eyebrows, muzzle, and legs as commonly seen in the Doberman Pinscher and
the Rottweiler. This gene modifies the A Locus at allele, so dogs that do not express at are not influenced
by this gene.

S Locus (MITF)

The S Locus determines white spotting and pigment distribution. MITF controls where pigment is
produced, and an insertion in the MITF gene causes a loss of pigment in the coat and skin, resulting in
Likely to have little to
white hair and/or pink skin. Dogs with two copies of this variant will likely have breed-dependent white
no white in coat (SS)
patterning, with a nearly white, parti, or piebald coat. Dogs with one copy of this variant will have more
limited white spotting and may be considered flash, parti or piebald. This MITF variant does not explain all
white spotting patterns in dogs and other variants are currently being researched. Some dogs may have
small amounts of white on the paws, chest, face, or tail regardless of their S Locus genotype.

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DNA Test Report Test Date: March 16th, 2023 embk.me/nwrssaltwaterpearls

TRAITS: COAT COLOR (CONTINUED)

TRAIT RESULT

M Locus (PMEL)

Merle coat patterning is common to several dog breeds including the Australian Shepherd, Catahoula
Leopard Dog, and Shetland Sheepdog, among many others. Merle arises from an unstable SINE insertion
(which we term the "M*" allele) that disrupts activity of the pigmentary gene PMEL, leading to mottled or
patchy coat color. Dogs with an M*m result are likely to be phenotypically merle or could be "non-
expressing" merle, meaning that the merle pattern is very subtle or not at all evident in their coat. Dogs
No merle alleles (mm)
with an M*M* result are likely to be phenotypically merle or double merle. Dogs with an mm result have no
merle alleles and are unlikely to have a merle coat pattern.

Note that Embark does not currently distinguish between the recently described cryptic, atypical,
atypical+, classic, and harlequin merle alleles. Our merle test only detects the presence, but not the length
of the SINE insertion. We do not recommend making breeding decisions on this result alone. Please pursue
further testing for allelic distinction prior to breeding decisions.

R Locus (USH2A) LINKAGE

The R Locus regulates the presence or absence of the roan coat color pattern. Partial duplication of the
USH2A gene is strongly associated with this coat pattern. Dogs with at least one R allele will likely have
roaning on otherwise uniformly unpigmented white areas. Roan appears in white areas controlled by the S Likely no impact on
Locus but not in other white or cream areas created by other loci, such as the E Locus with ee along with coat pattern (rr)
Dilute Red Pigmentation by I Locus (for example, in Samoyeds). Mechanisms for controlling the extent of
roaning are currently unknown, and roaning can appear in a uniform or non-uniform pattern. Further, non-
uniform roaning may appear as ticked, and not obviously roan. The roan pattern can appear with or without
ticking.

H Locus (Harlequin)

This pattern is recognized in Great Danes and causes dogs to have a white coat with patches of darker No harlequin alleles
pigment. A dog with an Hh result will be harlequin if they are also M*m or M*M* at the M Locus and are not (hh)
ee at the E locus. Dogs with a result of hh will not be harlequin. This trait is thought to be homozygous
lethal; a living dog with an HH genotype has never been found.

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TRAITS: OTHER COAT TRAITS

TRAIT RESULT

Furnishings (RSPO2) LINKAGE

Dogs with one or two copies of the F allele have “furnishings”: the mustache, beard, and eyebrows Likely unfurnished (no
characteristic of breeds like the Schnauzer, Scottish Terrier, and Wire Haired Dachshund. A dog with two I mustache, beard,
alleles will not have furnishings, which is sometimes called an “improper coat” in breeds where and/or eyebrows) (II)
furnishings are part of the breed standard. The mutation is a genetic insertion which we measure
indirectly using a linkage test highly correlated with the insertion.

Coat Length (FGF5)

The FGF5 gene is known to affect hair length in many different species, including cats, dogs, mice, and Likely short or mid-
humans. In dogs, the T allele confers a long, silky haircoat as observed in the Yorkshire Terrier and the length coat (GG)
Long Haired Whippet. The ancestral G allele causes a shorter coat as seen in the Boxer or the American
Staffordshire Terrier. In certain breeds (such as Corgi), the long haircoat is described as “fluff.”

Shedding (MC5R)

Dogs with at least one copy of the ancestral C allele, like many Labradors and German Shepherd Dogs, are Likely light shedding
heavy or seasonal shedders, while those with two copies of the T allele, including many Boxers, Shih Tzus (TT)
and Chihuahuas, tend to be lighter shedders. Dogs with furnished/wire-haired coats caused by RSPO2
(the furnishings gene) tend to be low shedders regardless of their genotype at this gene.

Hairlessness (FOXI3) LINKAGE

A duplication in the FOXI3 gene causes hairlessness over most of the body as well as changes in tooth
shape and number. This mutation occurs in Peruvian Inca Orchid, Xoloitzcuintli (Mexican Hairless), and Very unlikely to be
Chinese Crested (other hairless breeds have different mutations). Dogs with the NDup genotype are likely hairless (NN)
to be hairless while dogs with the NN genotype are likely to have a normal coat. The DupDup genotype has
never been observed, suggesting that dogs with that genotype cannot survive to birth. Please note that
this is a linkage test, so it may not be as predictive as direct tests of the mutation in some lines.

Hairlessness (SGK3)

Very unlikely to be
Hairlessness in the American Hairless Terrier arises from a mutation in the SGK3 gene. Dogs with the DD
hairless (NN)
result are likely to be hairless. Dogs with the ND genotype will have a normal coat, but can pass the D
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TRAITS: OTHER COAT TRAITS (CONTINUED)

TRAIT RESULT

Oculocutaneous Albinism Type 2 (SLC45A2) LINKAGE

Dogs with two copies DD of this deletion in the SLC45A2 gene have oculocutaneous albinism (OCA), also
known as Doberman Z Factor Albinism, a recessive condition characterized by severely reduced or absent
pigment in the eyes, skin, and hair. Affected dogs sometimes suffer from vision problems due to lack of eye Reduced pigmentation
pigment (which helps direct and absorb ambient light) and are prone to sunburn. Dogs with a single copy (DD)
of the deletion ND will not be affected but can pass the mutation on to their offspring. This particular
mutation can be traced back to a single white Doberman Pinscher born in 1976, and it has only been
observed in dogs descended from this individual. Please note that this is a linkage test, so it may not be as
predictive as direct tests of the mutation in some lines.

Coat Texture (KRT71)

Dogs with a long coat and at least one copy of the T allele have a wavy or curly coat characteristic of
Likely straight coat
Poodles and Bichon Frises. Dogs with two copies of the ancestral C allele are likely to have a straight coat,
(CC)
but there are other factors that can cause a curly coat, for example if they at least one F allele for the
Furnishings (RSPO2) gene then they are likely to have a curly coat. Dogs with short coats may carry one or
two copies of the T allele but still have straight coats.

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TRAITS: OTHER BODY FEATURES

TRAIT RESULT

Muzzle Length (BMP3)

Dogs in medium-length muzzle (mesocephalic) breeds like Staffordshire Terriers and Labradors, and long
muzzle (dolichocephalic) breeds like Whippet and Collie have one, or more commonly two, copies of the
ancestral C allele. Dogs in many short-length muzzle (brachycephalic) breeds such as the English Bulldog, Likely medium or long
Pug, and Pekingese have two copies of the derived A allele. At least five different genes affect muzzle muzzle (CC)
length in dogs, with BMP3 being the only one with a known causal mutation. For example, the skull shape
of some breeds, including the dolichocephalic Scottish Terrier or the brachycephalic Japanese Chin,
appear to be caused by other genes. Thus, dogs may have short or long muzzles due to other genetic
factors that are not yet known to science.

Tail Length (T)

Whereas most dogs have two C alleles and a long tail, dogs with one G allele are likely to have a bobtail,
which is an unusually short or absent tail. This mutation causes natural bobtail in many breeds including
the Pembroke Welsh Corgi, the Australian Shepherd, and the Brittany Spaniel. Dogs with GG genotypes Likely normal-length
have not been observed, suggesting that dogs with the GG genotype do not survive to birth. tail (CC)
Please note that this mutation does not explain every natural bobtail! While certain lineages of Boston
Terrier, English Bulldog, Rottweiler, Miniature Schnauzer, Cavalier King Charles Spaniel, and Parson Russell
Terrier, and Dobermans are born with a natural bobtail, these breeds do not have this mutation. This
suggests that other unknown genetic mutations can also lead to a natural bobtail.

Hind Dewclaws (LMBR1)

Common in certain breeds such as the Saint Bernard, hind dewclaws are extra, nonfunctional digits Unlikely to have hind
located midway between a dog's paw and hock. Dogs with at least one copy of the T allele have about a dew claws (CC)
50% chance of having hind dewclaws. Note that other (currently unknown to science) mutations can also
cause hind dewclaws, so some CC or TC dogs will have hind dewclaws.

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TRAITS: OTHER BODY FEATURES (CONTINUED)

TRAIT RESULT

Blue Eye Color (ALX4) LINKAGE

Embark researchers discovered this large duplication associated with blue eyes in Arctic breeds like
Siberian Husky as well as tri-colored (non-merle) Australian Shepherds. Dogs with at least one copy of the
Less likely to have blue
duplication (Dup) are more likely to have at least one blue eye. Some dogs with the duplication may have
eyes (NN)
only one blue eye (complete heterochromia) or may not have blue eyes at all; nevertheless, they can still
pass the duplication and the trait to their offspring. NN dogs do not carry this duplication, but may have
blue eyes due to other factors, such as merle. Please note that this is a linkage test, so it may not be as
predictive as direct tests of the mutation in some lines.

Back Muscling & Bulk, Large Breed (ACSL4)

The T allele is associated with heavy muscling along the back and trunk in characteristically "bulky" large-
breed dogs including the Saint Bernard, Bernese Mountain Dog, Greater Swiss Mountain Dog, and Likely normal muscling
Rottweiler. The “bulky” T allele is absent from leaner shaped large breed dogs like the Great Dane, Irish (CC)
Wolfhound, and Scottish Deerhound, which are fixed for the ancestral C allele. Note that this mutation does
not seem to affect muscling in small or even mid-sized dog breeds with notable back muscling, including
the American Staffordshire Terrier, Boston Terrier, and the English Bulldog.

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TRAITS: BODY SIZE

TRAIT RESULT

Body Size (IGF1)

Larger (NN)
The I allele is associated with smaller body size.

Body Size (IGFR1)

Larger (GG)
The A allele is associated with smaller body size.

Body Size (STC2)

Larger (TT)
The A allele is associated with smaller body size.

Body Size (GHR - E191K)

Larger (GG)
The A allele is associated with smaller body size.

Body Size (GHR - P177L)

Larger (CC)
The T allele is associated with smaller body size.

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TRAITS: PERFORMANCE
TRAIT RESULT

Altitude Adaptation (EPAS1)

Normal altitude
This mutation causes dogs to be especially tolerant of low oxygen environments (hypoxia), such as those
tolerance (GG)
found at high elevations. Dogs with at least one A allele are less susceptible to "altitude sickness." This
mutation was originally identified in breeds from high altitude areas such as the Tibetan Mastiff.

Appetite (POMC) LINKAGE

This mutation in the POMC gene is found primarily in Labrador and Flat Coated Retrievers. Compared to
dogs with no copies of the mutation (NN), dogs with one (ND) or two (DD) copies of the mutation are more Normal food
likely to have high food motivation, which can cause them to eat excessively, have higher body fat motivation (NN)
percentage, and be more prone to obesity. Read more about the genetics of POMC, and learn how you can
contribute to research, in our blog post (https://embarkvet.com/resources/blog/pomc-dogs/). We
measure this result using a linkage test.

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DNA Test Report Test Date: March 16th, 2023 embk.me/nwrssaltwaterpearls

HEALTH REPORT
How to interpret Pearl’s genetic health results:
If Pearl inherited any of the variants that we tested, they will be listed at the top of the Health Report section, along with a
description of how to interpret this result. We also include all of the variants that we tested Pearl for that we did not detect the risk
variant for.

A genetic test is not a diagnosis

This genetic test does not diagnose a disease. Please talk to your vet about your dog’s genetic results, or if you think that your pet
may have a health condition or disease.

Summary
Of the 254 genetic health risks we analyzed, we found 3 results that you should learn about.

Increased risk results (2)

Dilated Cardiomyopathy, DCM1

Dilated Cardiomyopathy, DCM2

Notable results (1)

ALT Activity

Clear results

Breed-relevant (5)

Other (246)

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BREED-RELEVANT RESULTS
Research studies indicate that these results are more relevant to dogs like Pearl, and may influence her chances of developing
certain health conditions.

Dilated Cardiomyopathy, DCM1 (PDK4, Doberman Pinscher Variant 1) Increased risk

Dilated Cardiomyopathy, DCM2 (TTN, Doberman Pinscher Variant 2) Increased risk

Deafness and Vestibular Syndrome of Dobermans, DVDob, DINGS (MYO7A) Clear

Ehlers Danlos (ADAMTS2, Doberman Pinscher Variant) Clear

Narcolepsy (HCRTR2 Intron 4, Doberman Pinscher Variant) Clear

Unilateral Deafness and Vestibular Syndrome (PTPRQ Exon 39, Doberman Pinscher) Clear

Von Willebrand Disease Type I, Type I vWD (VWF) Clear

Registration: American Kennel Club (AKC)

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OTHER RESULTS
Research has not yet linked these conditions to dogs with similar breeds to Pearl. Review any increased risk or notable results to
understand her potential risk and recommendations.

ALT Activity (GPT) Notable

2-DHA Kidney & Bladder Stones (APRT) Clear

Acral Mutilation Syndrome (GDNF-AS, Spaniel and Pointer Variant) Clear

Alaskan Husky Encephalopathy (SLC19A3) Clear

Alaskan Malamute Polyneuropathy, AMPN (NDRG1 SNP) Clear

Alexander Disease (GFAP) Clear

Anhidrotic Ectodermal Dysplasia (EDA Intron 8) Clear

Autosomal Dominant Progressive Retinal Atrophy (RHO) Clear

Bald Thigh Syndrome (IGFBP5) Clear

Bernard-Soulier Syndrome, BSS (GP9, Cocker Spaniel Variant) Clear

Bully Whippet Syndrome (MSTN) Clear

Canine Elliptocytosis (SPTB Exon 30) Clear

Canine Fucosidosis (FUCA1) Clear

Canine Leukocyte Adhesion Deficiency Type I, CLAD I (ITGB2, Setter Variant) Clear

Canine Leukocyte Adhesion Deficiency Type III, CLAD III (FERMT3, German Shepherd Variant) Clear

Canine Multifocal Retinopathy, cmr1 (BEST1 Exon 2) Clear

Canine Multifocal Retinopathy, cmr2 (BEST1 Exon 5, Coton de Tulear Variant) Clear

Canine Multifocal Retinopathy, cmr3 (BEST1 Exon 10 Deletion, Finnish and Swedish Lapphund, Clear
Lapponian Herder Variant)
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OTHER RESULTS
Canine Multiple System Degeneration (SERAC1 Exon 4, Chinese Crested Variant) Clear

Canine Multiple System Degeneration (SERAC1 Exon 15, Kerry Blue Terrier Variant) Clear

Cardiomyopathy and Juvenile Mortality (YARS2) Clear

Centronuclear Myopathy, CNM (PTPLA) Clear

Cerebellar Hypoplasia (VLDLR, Eurasier Variant) Clear

Chondrodystrophy (ITGA10, Norwegian Elkhound and Karelian Bear Dog Variant) Clear

Cleft Lip and/or Cleft Palate (ADAMTS20, Nova Scotia Duck Tolling Retriever Variant) Clear

Cleft Palate, CP1 (DLX6 intron 2, Nova Scotia Duck Tolling Retriever Variant) Clear

Cobalamin Malabsorption (CUBN Exon 8, Beagle Variant) Clear

Cobalamin Malabsorption (CUBN Exon 53, Border Collie Variant) Clear

Collie Eye Anomaly (NHEJ1) Clear

Complement 3 Deficiency, C3 Deficiency (C3) Clear

Congenital Cornification Disorder (NSDHL, Chihuahua Variant) Clear

Congenital Hypothyroidism (TPO, Rat, Toy, Hairless Terrier Variant) Clear

Congenital Hypothyroidism (TPO, Tenterfield Terrier Variant) Clear

Congenital Hypothyroidism with Goiter (TPO Intron 13, French Bulldog Variant) Clear

Congenital Hypothyroidism with Goiter (SLC5A5, Shih Tzu Variant) Clear

Congenital Macrothrombocytopenia (TUBB1 Exon 1, Cairn and Norfolk Terrier Variant) Clear

Registration: American Kennel Club (AKC)

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OTHER RESULTS
Congenital Myasthenic Syndrome, CMS (COLQ, Labrador Retriever Variant) Clear

Congenital Myasthenic Syndrome, CMS (COLQ, Golden Retriever Variant) Clear

Congenital Myasthenic Syndrome, CMS (CHAT, Old Danish Pointing Dog Variant) Clear

Congenital Myasthenic Syndrome, CMS (CHRNE, Jack Russell Terrier Variant) Clear

Congenital Stationary Night Blindness (LRIT3, Beagle Variant) Clear

Congenital Stationary Night Blindness (RPE65, Briard Variant) Clear

Craniomandibular Osteopathy, CMO (SLC37A2) Clear

Craniomandibular Osteopathy, CMO (SLC37A2 Intron 16, Basset Hound Variant) Clear

Cystinuria Type I-A (SLC3A1, Newfoundland Variant) Clear

Cystinuria Type II-A (SLC3A1, Australian Cattle Dog Variant) Clear

Cystinuria Type II-B (SLC7A9, Miniature Pinscher Variant) Clear

Day Blindness (CNGB3 Deletion, Alaskan Malamute Variant) Clear

Day Blindness (CNGA3 Exon 7, German Shepherd Variant) Clear

Day Blindness (CNGA3 Exon 7, Labrador Retriever Variant) Clear

Day Blindness (CNGB3 Exon 6, German Shorthaired Pointer Variant) Clear

Degenerative Myelopathy, DM (SOD1A) Clear

Demyelinating Polyneuropathy (SBF2/MTRM13) Clear

Dental-Skeletal-Retinal Anomaly (MIA3, Cane Corso Variant) Clear

Registration: American Kennel Club (AKC)

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DNA Test Report Test Date: March 16th, 2023 embk.me/nwrssaltwaterpearls

OTHER RESULTS
Diffuse Cystic Renal Dysplasia and Hepatic Fibrosis (INPP5E Intron 9, Norwich Terrier Variant) Clear

Dilated Cardiomyopathy, DCM (RBM20, Schnauzer Variant) Clear

Disproportionate Dwarfism (PRKG2, Dogo Argentino Variant) Clear

Dry Eye Curly Coat Syndrome (FAM83H Exon 5) Clear

Dystrophic Epidermolysis Bullosa (COL7A1, Central Asian Shepherd Dog Variant) Clear

Dystrophic Epidermolysis Bullosa (COL7A1, Golden Retriever Variant) Clear

Early Bilateral Deafness (LOXHD1 Exon 38, Rottweiler Variant) Clear

Early Onset Adult Deafness, EOAD (EPS8L2 Deletion, Rhodesian Ridgeback Variant) Clear

Early Onset Cerebellar Ataxia (SEL1L, Finnish Hound Variant) Clear

Enamel Hypoplasia (ENAM Deletion, Italian Greyhound Variant) Clear

Enamel Hypoplasia (ENAM SNP, Parson Russell Terrier Variant) Clear

Episodic Falling Syndrome (BCAN) Clear

Exercise-Induced Collapse, EIC (DNM1) Clear

Factor VII Deficiency (F7 Exon 5) Clear

Factor XI Deficiency (F11 Exon 7, Kerry Blue Terrier Variant) Clear

Familial Nephropathy (COL4A4 Exon 3, Cocker Spaniel Variant) Clear

Familial Nephropathy (COL4A4 Exon 30, English Springer Spaniel Variant) Clear

Fanconi Syndrome (FAN1, Basenji Variant) Clear

Registration: American Kennel Club (AKC)

“PEARL”
NWR'S SALTWATER PEARL

DNA Test Report Test Date: March 16th, 2023 embk.me/nwrssaltwaterpearls

OTHER RESULTS
Fetal-Onset Neonatal Neuroaxonal Dystrophy (MFN2, Giant Schnauzer Variant) Clear

Glanzmann's Thrombasthenia Type I (ITGA2B Exon 13, Great Pyrenees Variant) Clear

Glanzmann's Thrombasthenia Type I (ITGA2B Exon 12, Otterhound Variant) Clear

Globoid Cell Leukodystrophy, Krabbe disease (GALC Exon 5, Terrier Variant) Clear

Glycogen Storage Disease Type IA, Von Gierke Disease, GSD IA (G6PC, Maltese Variant) Clear

Glycogen Storage Disease Type IIIA, GSD IIIA (AGL, Curly Coated Retriever Variant) Clear

Glycogen storage disease Type VII, Phosphofructokinase Deficiency, PFK Deficiency (PFKM, Whippet Clear
and English Springer Spaniel Variant)

Glycogen storage disease Type VII, Phosphofructokinase Deficiency, PFK Deficiency (PFKM, Clear
Wachtelhund Variant)

GM1 Gangliosidosis (GLB1 Exon 2, Portuguese Water Dog Variant) Clear

GM1 Gangliosidosis (GLB1 Exon 15, Shiba Inu Variant) Clear

GM1 Gangliosidosis (GLB1 Exon 15, Alaskan Husky Variant) Clear

GM2 Gangliosidosis (HEXA, Japanese Chin Variant) Clear

GM2 Gangliosidosis (HEXB, Poodle Variant) Clear

Golden Retriever Progressive Retinal Atrophy 1, GR-PRA1 (SLC4A3) Clear

Golden Retriever Progressive Retinal Atrophy 2, GR-PRA2 (TTC8) Clear

Goniodysgenesis and Glaucoma, Pectinate Ligament Dysplasia, PLD (OLFM3) Clear

Hemophilia A (F8 Exon 11, German Shepherd Variant 1) Clear

Hemophilia A (F8 Exon 1, German Shepherd Variant 2) Clear

Registration: American Kennel Club (AKC)

“PEARL”
NWR'S SALTWATER PEARL

DNA Test Report Test Date: March 16th, 2023 embk.me/nwrssaltwaterpearls

OTHER RESULTS
Hemophilia A (F8 Exon 10, Boxer Variant) Clear

Hemophilia B (F9 Exon 7, Terrier Variant) Clear

Hemophilia B (F9 Exon 7, Rhodesian Ridgeback Variant) Clear

Hereditary Ataxia, Cerebellar Degeneration (RAB24, Old English Sheepdog and Gordon Setter Variant) Clear

Hereditary Cataracts (HSF4 Exon 9, Australian Shepherd Variant) Clear

Hereditary Footpad Hyperkeratosis (FAM83G, Terrier and Kromfohrlander Variant) Clear

Hereditary Footpad Hyperkeratosis (DSG1, Rottweiler Variant) Clear

Hereditary Nasal Parakeratosis (SUV39H2 Intron 4, Greyhound Variant) Clear

Hereditary Nasal Parakeratosis, HNPK (SUV39H2) Clear

Hereditary Vitamin D-Resistant Rickets (VDR) Clear

Hypocatalasia, Acatalasemia (CAT) Clear

Hypomyelination and Tremors (FNIP2, Weimaraner Variant) Clear

Hypophosphatasia (ALPL Exon 9, Karelian Bear Dog Variant) Clear

Ichthyosis (NIPAL4, American Bulldog Variant) Clear

Ichthyosis (ASPRV1 Exon 2, German Shepherd Variant) Clear

Ichthyosis (SLC27A4, Great Dane Variant) Clear

Ichthyosis, Epidermolytic Hyperkeratosis (KRT10, Terrier Variant) Clear

Ichthyosis, ICH1 (PNPLA1, Golden Retriever Variant) Clear

Registration: American Kennel Club (AKC)

“PEARL”
NWR'S SALTWATER PEARL

DNA Test Report Test Date: March 16th, 2023 embk.me/nwrssaltwaterpearls

OTHER RESULTS
Inflammatory Myopathy (SLC25A12) Clear

Inherited Myopathy of Great Danes (BIN1) Clear

Inherited Selected Cobalamin Malabsorption with Proteinuria (CUBN, Komondor Variant) Clear

Intervertebral Disc Disease (Type I) (FGF4 retrogene - CFA12) Clear

Intestinal Lipid Malabsorption (ACSL5, Australian Kelpie) Clear

Junctional Epidermolysis Bullosa (LAMA3 Exon 66, Australian Cattle Dog Variant) Clear

Junctional Epidermolysis Bullosa (LAMB3 Exon 11, Australian Shepherd Variant) Clear

Juvenile Epilepsy (LGI2) Clear

Juvenile Laryngeal Paralysis and Polyneuropathy (RAB3GAP1, Rottweiler Variant) Clear

Juvenile Myoclonic Epilepsy (DIRAS1) Clear

L-2-Hydroxyglutaricaciduria, L2HGA (L2HGDH, Staffordshire Bull Terrier Variant) Clear

Lagotto Storage Disease (ATG4D) Clear

Laryngeal Paralysis (RAPGEF6, Miniature Bull Terrier Variant) Clear

Late Onset Spinocerebellar Ataxia (CAPN1) Clear

Late-Onset Neuronal Ceroid Lipofuscinosis, NCL 12 (ATP13A2, Australian Cattle Dog Variant) Clear

Leonberger Polyneuropathy 1 (LPN1, ARHGEF10) Clear

Leonberger Polyneuropathy 2 (GJA9) Clear

Lethal Acrodermatitis, LAD (MKLN1) Clear

Registration: American Kennel Club (AKC)

“PEARL”
NWR'S SALTWATER PEARL

DNA Test Report Test Date: March 16th, 2023 embk.me/nwrssaltwaterpearls

OTHER RESULTS
Leukodystrophy (TSEN54 Exon 5, Standard Schnauzer Variant) Clear

Ligneous Membranitis, LM (PLG) Clear

Limb Girdle Muscular Dystrophy (SGCD, Boston Terrier Variant) Clear

Limb-Girdle Muscular Dystrophy 2D (SGCA Exon 3, Miniature Dachshund Variant) Clear

Long QT Syndrome (KCNQ1) Clear

Lundehund Syndrome (LEPREL1) Clear

Macular Corneal Dystrophy, MCD (CHST6) Clear

Malignant Hyperthermia (RYR1) Clear

May-Hegglin Anomaly (MYH9) Clear

Methemoglobinemia (CYB5R3, Pit Bull Terrier Variant) Clear

Methemoglobinemia (CYB5R3) Clear

Microphthalmia (RBP4 Exon 2, Soft Coated Wheaten Terrier Variant) Clear

Mucopolysaccharidosis IIIB, Sanfilippo Syndrome Type B, MPS IIIB (NAGLU, Schipperke Variant) Clear

Mucopolysaccharidosis Type IIIA, Sanfilippo Syndrome Type A, MPS IIIA (SGSH Exon 6, Dachshund Clear
Variant)

Mucopolysaccharidosis Type IIIA, Sanfilippo Syndrome Type A, MPS IIIA (SGSH Exon 6, New Zealand Clear
Huntaway Variant)

Mucopolysaccharidosis Type VI, Maroteaux-Lamy Syndrome, MPS VI (ARSB Exon 5, Miniature Pinscher Clear
Variant)

Mucopolysaccharidosis Type VII, Sly Syndrome, MPS VII (GUSB Exon 3, German Shepherd Variant) Clear

Mucopolysaccharidosis Type VII, Sly Syndrome, MPS VII (GUSB Exon 5, Terrier Brasileiro Variant) Clear

Registration: American Kennel Club (AKC)

“PEARL”
NWR'S SALTWATER PEARL

DNA Test Report Test Date: March 16th, 2023 embk.me/nwrssaltwaterpearls

OTHER RESULTS
Multiple Drug Sensitivity (ABCB1) Clear

Muscular Dystrophy (DMD, Cavalier King Charles Spaniel Variant 1) Clear

Muscular Dystrophy (DMD, Golden Retriever Variant) Clear

Musladin-Lueke Syndrome, MLS (ADAMTSL2) Clear

Myasthenia Gravis-Like Syndrome (CHRNE, Heideterrier Variant) Clear

Myotonia Congenita (CLCN1 Exon 23, Australian Cattle Dog Variant) Clear

Myotonia Congenita (CLCN1 Exon 7, Miniature Schnauzer Variant) Clear

Narcolepsy (HCRTR2 Exon 1, Dachshund Variant) Clear

Narcolepsy (HCRTR2 Intron 6, Labrador Retriever Variant) Clear

Nemaline Myopathy (NEB, American Bulldog Variant) Clear

Neonatal Cerebellar Cortical Degeneration (SPTBN2, Beagle Variant) Clear

Neonatal Encephalopathy with Seizures, NEWS (ATF2) Clear

Neonatal Interstitial Lung Disease (LAMP3) Clear

Neuroaxonal Dystrophy, NAD (VPS11, Rottweiler Variant) Clear

Neuroaxonal Dystrophy, NAD (TECPR2, Spanish Water Dog Variant) Clear

Neuronal Ceroid Lipofuscinosis 1, NCL 1 (PPT1 Exon 8, Dachshund Variant 1) Clear

Neuronal Ceroid Lipofuscinosis 10, NCL 10 (CTSD Exon 5, American Bulldog Variant) Clear

Neuronal Ceroid Lipofuscinosis 2, NCL 2 (TPP1 Exon 4, Dachshund Variant 2) Clear

Registration: American Kennel Club (AKC)

“PEARL”
NWR'S SALTWATER PEARL

DNA Test Report Test Date: March 16th, 2023 embk.me/nwrssaltwaterpearls

OTHER RESULTS
Neuronal Ceroid Lipofuscinosis 5, NCL 5 (CLN5 Exon 4 SNP, Border Collie Variant) Clear

Neuronal Ceroid Lipofuscinosis 5, NCL 5 (CLN5 Exon 4 Deletion, Golden Retriever Variant) Clear

Neuronal Ceroid Lipofuscinosis 6, NCL 6 (CLN6 Exon 7, Australian Shepherd Variant) Clear

Neuronal Ceroid Lipofuscinosis 7, NCL 7 (MFSD8, Chihuahua and Chinese Crested Variant) Clear

Neuronal Ceroid Lipofuscinosis 8, NCL 8 (CLN8, Australian Shepherd Variant) Clear

Neuronal Ceroid Lipofuscinosis 8, NCL 8 (CLN8 Exon 2, English Setter Variant) Clear

Neuronal Ceroid Lipofuscinosis 8, NCL 8 (CLN8 Insertion, Saluki Variant) Clear

Neuronal Ceroid Lipofuscinosis, Cerebellar Ataxia, NCL4A (ARSG Exon 2, American Staffordshire Terrier Clear
Variant)

Oculocutaneous Albinism, OCA (SLC45A2 Exon 6, Bullmastiff Variant) Clear

Oculoskeletal Dysplasia 2 (COL9A2, Samoyed Variant) Clear

Osteochondrodysplasia (SLC13A1, Poodle Variant) Clear

Osteogenesis Imperfecta (COL1A2, Beagle Variant) Clear

Osteogenesis Imperfecta (SERPINH1, Dachshund Variant) Clear

Osteogenesis Imperfecta (COL1A1, Golden Retriever Variant) Clear

P2Y12 Receptor Platelet Disorder (P2Y12) Clear

Pachyonychia Congenita (KRT16, Dogue de Bordeaux Variant) Clear

Paroxysmal Dyskinesia, PxD (PIGN) Clear

Persistent Mullerian Duct Syndrome, PMDS (AMHR2) Clear

Registration: American Kennel Club (AKC)

“PEARL”
NWR'S SALTWATER PEARL

DNA Test Report Test Date: March 16th, 2023 embk.me/nwrssaltwaterpearls

OTHER RESULTS
Pituitary Dwarfism (POU1F1 Intron 4, Karelian Bear Dog Variant) Clear

Platelet Factor X Receptor Deficiency, Scott Syndrome (TMEM16F) Clear

Polycystic Kidney Disease, PKD (PKD1) Clear

Pompe's Disease (GAA, Finnish and Swedish Lapphund, Lapponian Herder Variant) Clear

Prekallikrein Deficiency (KLKB1 Exon 8) Clear

Primary Ciliary Dyskinesia, PCD (NME5, Alaskan Malamute Variant) Clear

Primary Ciliary Dyskinesia, PCD (CCDC39 Exon 3, Old English Sheepdog Variant) Clear

Primary Hyperoxaluria (AGXT) Clear

Primary Lens Luxation (ADAMTS17) Clear

Primary Open Angle Glaucoma (ADAMTS17 Exon 11, Basset Fauve de Bretagne Variant) Clear

Primary Open Angle Glaucoma (ADAMTS10 Exon 17, Beagle Variant) Clear

Primary Open Angle Glaucoma (ADAMTS10 Exon 9, Norwegian Elkhound Variant) Clear

Primary Open Angle Glaucoma and Primary Lens Luxation (ADAMTS17 Exon 2, Chinese Shar-Pei Clear
Variant)

Progressive Retinal Atrophy (SAG) Clear

Progressive Retinal Atrophy (IFT122 Exon 26, Lapponian Herder Variant) Clear

Progressive Retinal Atrophy, Bardet-Biedl Syndrome (BBS2 Exon 11, Shetland Sheepdog Variant) Clear

Progressive Retinal Atrophy, CNGA (CNGA1 Exon 9) Clear

Progressive Retinal Atrophy, crd1 (PDE6B, American Staffordshire Terrier Variant) Clear

Registration: American Kennel Club (AKC)

“PEARL”
NWR'S SALTWATER PEARL

DNA Test Report Test Date: March 16th, 2023 embk.me/nwrssaltwaterpearls

OTHER RESULTS
Progressive Retinal Atrophy, crd4/cord1 (RPGRIP1) Clear

Progressive Retinal Atrophy, PRA1 (CNGB1) Clear

Progressive Retinal Atrophy, PRA3 (FAM161A) Clear

Progressive Retinal Atrophy, prcd (PRCD Exon 1) Clear

Progressive Retinal Atrophy, rcd1 (PDE6B Exon 21, Irish Setter Variant) Clear

Progressive Retinal Atrophy, rcd3 (PDE6A) Clear

Proportionate Dwarfism (GH1 Exon 5, Chihuahua Variant) Clear

Protein Losing Nephropathy, PLN (NPHS1) Clear

Pyruvate Dehydrogenase Deficiency (PDP1, Spaniel Variant) Clear

Pyruvate Kinase Deficiency (PKLR Exon 5, Basenji Variant) Clear

Pyruvate Kinase Deficiency (PKLR Exon 7, Beagle Variant) Clear

Pyruvate Kinase Deficiency (PKLR Exon 10, Terrier Variant) Clear

Pyruvate Kinase Deficiency (PKLR Exon 7, Labrador Retriever Variant) Clear

Pyruvate Kinase Deficiency (PKLR Exon 7, Pug Variant) Clear

Raine Syndrome (FAM20C) Clear

Recurrent Inflammatory Pulmonary Disease, RIPD (AKNA, Rough Collie Variant) Clear

Renal Cystadenocarcinoma and Nodular Dermatofibrosis (FLCN Exon 7) Clear

Retina Dysplasia and/or Optic Nerve Hypoplasia (SIX6 Exon 1, Golden Retriever Variant) Clear

Registration: American Kennel Club (AKC)

“PEARL”
NWR'S SALTWATER PEARL

DNA Test Report Test Date: March 16th, 2023 embk.me/nwrssaltwaterpearls

OTHER RESULTS
Sensory Neuropathy (FAM134B, Border Collie Variant) Clear

Severe Combined Immunodeficiency, SCID (PRKDC, Terrier Variant) Clear

Severe Combined Immunodeficiency, SCID (RAG1, Wetterhoun Variant) Clear

Shaking Puppy Syndrome (PLP1, English Springer Spaniel Variant) Clear

Shar-Pei Autoinflammatory Disease, SPAID, Shar-Pei Fever (MTBP) Clear

Skeletal Dysplasia 2, SD2 (COL11A2, Labrador Retriever Variant) Clear

Skin Fragility Syndrome (PKP1, Chesapeake Bay Retriever Variant) Clear

Spinocerebellar Ataxia (SCN8A, Alpine Dachsbracke Variant) Clear

Spinocerebellar Ataxia with Myokymia and/or Seizures (KCNJ10) Clear

Spongy Degeneration with Cerebellar Ataxia 1 (KCNJ10) Clear

Spongy Degeneration with Cerebellar Ataxia 2 (ATP1B2) Clear

Stargardt Disease (ABCA4 Exon 28, Labrador Retriever Variant) Clear

Succinic Semialdehyde Dehydrogenase Deficiency (ALDH5A1 Exon 7, Saluki Variant) Clear

Thrombopathia (RASGRP1 Exon 5, American Eskimo Dog Variant) Clear

Thrombopathia (RASGRP1 Exon 5, Basset Hound Variant) Clear

Thrombopathia (RASGRP1 Exon 8, Landseer Variant) Clear

Trapped Neutrophil Syndrome, TNS (VPS13B) Clear

Ullrich-like Congenital Muscular Dystrophy (COL6A3 Exon 10, Labrador Retriever Variant) Clear

Registration: American Kennel Club (AKC)

“PEARL”
NWR'S SALTWATER PEARL

DNA Test Report Test Date: March 16th, 2023 embk.me/nwrssaltwaterpearls

OTHER RESULTS
Ullrich-like Congenital Muscular Dystrophy (COL6A1 Exon 3, Landseer Variant) Clear

Urate Kidney & Bladder Stones (SLC2A9) Clear

Von Willebrand Disease Type II, Type II vWD (VWF, Pointer Variant) Clear

Von Willebrand Disease Type III, Type III vWD (VWF Exon 4, Terrier Variant) Clear

Von Willebrand Disease Type III, Type III vWD (VWF Intron 16, Nederlandse Kooikerhondje Variant) Clear

Von Willebrand Disease Type III, Type III vWD (VWF Exon 7, Shetland Sheepdog Variant) Clear

X-Linked Hereditary Nephropathy, XLHN (COL4A5 Exon 35, Samoyed Variant 2) Clear

X-Linked Myotubular Myopathy (MTM1, Labrador Retriever Variant) Clear

X-Linked Progressive Retinal Atrophy 1, XL-PRA1 (RPGR) Clear

X-linked Severe Combined Immunodeficiency, X-SCID (IL2RG Exon 1, Basset Hound Variant) Clear

X-linked Severe Combined Immunodeficiency, X-SCID (IL2RG, Corgi Variant) Clear

Xanthine Urolithiasis (XDH, Mixed Breed Variant) Clear

β-Mannosidosis (MANBA Exon 16, Mixed-Breed Variant) Clear

Registration: American Kennel Club (AKC)

 “PEARL”
NWR'S SALTWATER PEARL

DNA Test Report Test Date: March 16th, 2023 embk.me/nwrssaltwaterpearls

HEALTH REPORT

Increased risk result

Dilated Cardiomyopathy, DCM1

NWR's Saltwater Pearl inherited one copy of the variant we tested for Dilated Cardiomyopathy, DCM1
Pearl is at increased risk for DCM1

How to interpret this result

Pearl has one copy of a variant in the PDK4 gene associated with increased risk for DCM in the American Doberman Pinscher. This
variant, also referred to as DCM1, is inherited in a dominant manner, meaning having one or two copies of this variant is thought to
confer the same amount of risk. However, the variant is thought to have incomplete penetrance: That is, not all dogs with this
variant will ultimately show signs of DCM. Moreover, the impact of this variant in other breeds of dog besides the Doberman has yet
to be fully understood. However, if your veterinarian thinks Pearl shows signs of having DCM based on their diagnostic testing, you
now have the opportunity to discuss early treatment. Please consult with your veterinarian regarding a diagnostic and treatment
plan for Pearl.

What is Dilated Cardiomyopathy, DCM1?

DCM is the most common acquired heart disease of adult dogs. The heart has two heavily muscled ventricles that pump blood away
from the heart. This disease causes progressive weakening of the ventricles by reducing the muscle mass, which causes the
ventricles to dilate. Dilated ventricles do not contract and circulate oxygenated blood well, which eventually leads to heart failure.

When signs & symptoms develop in affected dogs

This disease can rarely be seen in puppies and young adults. It is typically seen in middle aged to older dogs.

Signs & symptoms

In the early stages of DCM, you will likely not notice any changes in your dog. DCM typically presents at the end stages of the
disease, when the heart is failing. Signs include weakness, cold toes and ears, blue-grey gums and tongue, and respiratory distress.
If you see these signs, take your dog immediately to an emergency veterinarian!

How vets diagnose this condition

The earlier a diagnosis can be reached, the better the outcome. If you are concerned about your dog’s heart, discuss it with your
veterinarian who can run basic preliminary tests. They may recommend a visit to a veterinary cardiologist for a complete evaluation,
including an ultrasound of the heart (echocardiogram).

How this condition is treated

Treatment is completely dependent on how advanced the disease is at the time of diagnosis. It can range from monitoring the
patient periodically to intensive hospitalization at specialty veterinary practices.

Actions to take if your dog is affected

The cause of this disease is multifactorial and not completely understood. Genetics, nutrition, infections and environmental
exposures can all play a role in the development of DCM. In fact, DCM has recently been featured extensively in the news due to
suspected nutritional deficiencies in some grain free diets.
A l h di b b d tifi d di l i t d l H lt it i th b t t di DCM l
Registration:
 “PEARL”
NWR'S SALTWATER PEARL

DNA Test Report Test Date: March 16th, 2023 embk.me/nwrssaltwaterpearls

HEALTH REPORT

Increased risk result

Dilated Cardiomyopathy, DCM2

NWR's Saltwater Pearl inherited one copy of the variant we tested for Dilated Cardiomyopathy, DCM2
Pearl is at increased risk for DCM2

How to interpret this result

Pearl has one copy of a variant in the TTN gene associated with increased risk for DCM in the American Doberman Pinscher. This
variant, also referred to as DCM2, is inherited in a dominant manner, meaning having one or two copies of this variant is thought to
confer the same amount of risk. However, the variant is thought to have incomplete penetrance: That is, not all dogs with this
variant will ultimately show signs of DCM. Moreover, the impact of this variant in other breeds of dog besides the Doberman has yet
to be fully understood. However, if your veterinarian thinks Pearl shows signs of having DCM based on their diagnostic testing, you
now have the opportunity to discuss early treatment. Please consult with your veterinarian regarding a diagnostic and treatment
plan for Pearl.

What is Dilated Cardiomyopathy, DCM2?

DCM is the most common acquired heart disease of adult dogs. The heart has two heavily muscled ventricles that pump blood away
from the heart. This disease causes progressive weakening of the ventricles by reducing the muscle mass, which causes the
ventricles to dilate. Dilated ventricles do not contract and circulate oxygenated blood well, which eventually leads to heart failure.

When signs & symptoms develop in affected dogs

This disease can rarely be seen in puppies and young adults. It is typically seen in middle aged to older dogs.

Signs & symptoms

In the early stages of DCM, you will likely not notice any changes in your dog. DCM typically presents at the end stages of the
disease, when the heart is failing. Signs include weakness, cold toes and ears, blue-grey gums and tongue, and respiratory distress.
If you see these signs, take your dog immediately to an emergency veterinarian!

How vets diagnose this condition

The earlier a diagnosis can be reached, the better the outcome. If you are concerned about your dog’s heart, discuss it with your
veterinarian who can run basic preliminary tests. They may recommend a visit to a veterinary cardiologist for a complete evaluation,
including an ultrasound of the heart (echocardiogram).

How this condition is treated

Treatment is completely dependent on how advanced the disease is at the time of diagnosis. It can range from monitoring the
patient periodically to intensive hospitalization at specialty veterinary practices.

Actions to take if your dog is affected

The cause of this disease is multifactorial and not completely understood. Genetics, nutrition, infections and environmental
exposures can all play a role in the development of DCM. In fact, DCM has recently been featured extensively in the news due to
suspected nutritional deficiencies in some grain free diets.
A l h di b b d tifi d di l i t d l H lt it i th b t t di DCM l
Registration:
 “PEARL”
NWR'S SALTWATER PEARL

DNA Test Report Test Date: March 16th, 2023 embk.me/nwrssaltwaterpearls

HEALTH REPORT

Notable result

ALT Activity

NWR's Saltwater Pearl inherited both copies of the variant we tested for Alanine Aminotransferase Activity

Why is this important to your vet?

Pearl has two copies of a variant in the GPT gene and is likely to have a lower than average baseline ALT activity. ALT is a commonly
used measure of liver health on routine veterinary blood chemistry panels. As such, your veterinarian may want to watch for changes
in Pearl's ALT activity above their current, healthy, ALT activity. As an increase above Pearl’s baseline ALT activity could be evidence
of liver damage, even if it is within normal limits by standard ALT reference ranges.

What is Alanine Aminotransferase Activity?

Alanine aminotransferase (ALT) is a clinical tool that can be used by veterinarians to better monitor liver health. This result is not
associated with liver disease. ALT is one of several values veterinarians measure on routine blood work to evaluate the liver. It is a
naturally occurring enzyme located in liver cells that helps break down protein. When the liver is damaged or inflamed, ALT is
released into the bloodstream.

How vets diagnose this condition

Genetic testing is the only way to provide your veterinarian with this clinical tool.

How this condition is treated

Veterinarians may recommend blood work to establish a baseline ALT value for healthy dogs with one or two copies of this variant.

Registration:
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DNA Test Report Test Date: March 16th, 2023 embk.me/nwrssaltwaterpearls

INBREEDING AND DIVERSITY

CATEGORY RESULT

28%
Coefficient Of Inbreeding

Our genetic COI measures the proportion of your dog's genome where the genes on the mother’s side are
identical by descent to those on the father’s side.

High Diversity
MHC Class II - DLA DRB1
How common is this
amount of diversity in
A Dog Leukocyte Antigen (DLA) gene, DRB1 encodes a major histocompatibility complex (MHC) protein
purebreds:
involved in the immune response. Some studies have shown associations between certain DRB1
haplotypes and autoimmune diseases such as Addison's disease (hypoadrenocorticism) in certain dog
breeds, but these findings have yet to be scientifically validated.

High Diversity

MHC Class II - DLA DQA1 and DQB1 How common is this

amount of diversity in
DQA1 and DQB1 are two tightly linked DLA genes that code for MHC proteins involved in the immune purebreds:
response. A number of studies have shown correlations of DQA-DQB1 haplotypes and certain autoimmune
diseases; however, these have not yet been scientifically validated.

Registration: American Kennel Club

(AKC)