Microplate Manager 6 Software: Instruction Manual - Version 6.1
Microplate Manager 6 Software: Instruction Manual - Version 6.1
i
Introduction
Table of Contents
Table of Contents........................................................... ii
Introduction .................................................................... 1
Microplate Manager 6 Overview............................................................1
Menu Bars and Icons.............................................................................2
File Menu .................................................................................................. 3
Window Menu ........................................................................................... 4
ii
Microplate Manager 6
iii
Introduction
Producing Reports....................................................... 58
Setting Up Reports ..............................................................................58
Printing Reports...................................................................................61
Customizing Reports ...........................................................................63
Appendix....................................................................... 63
Appendix....................................................................... 64
References ..........................................................................................64
iv
Microplate Manager 6
v
Introduction
Introduction
This manual assumes that you are familiar with the standard commands and
functions associated with the Windows® or Mac® operating system, such as
opening, closing, and saving files, and moving and clicking your mouse.
Some of the features and functions in Microplate Manger 6 will be slightly different
depending on which microplate reader you are using (xMark or iMark). These
differences are described in the text.
1
Introduction
2
Introduction
File Menu
After creating a New Experiment or opening an existing data file, the File Menu
will list the main program functions.
The nine icons grouped at the left end of the Main Toolbar correspond to the same
function items listed under the File Menu.
3
Introduction
Window Menu
After creating a New Experiment or opening an existing data file, the Window
Menu will list the display functions for raw data, results and reports.
The six icons grouped at the right end of the Main Toolbar correspond to the same
function items listed under the Window Menu.
4
Installation and Instrument Setup
5
Installation and Instrument Setup
A list of filters installed in the iMark are automatically sent to the computer when
iMark is selected. The available filters display in the Wavelength area.
6
Installation and Instrument Setup
¾ If filters are added, removed, or their positions changed, the changes must be entered
on the iMark reader keypad, so that filters are displayed properly in MPM 6.
Entering Filters
On the iMark Keypad:
1. Click the Edit button.
2. Press down arrow button 2 times to select Filters, and press the Enter button.
3. Using the keypad, select numbers by using arrow buttons to move through
positions 1-8.
4. Press the Enter button to save settings.
5. Press Main button to put reader back into ready mode.
In Microplate Manager 6:
6. Click Reload Filter on the Instrument Setup dialog.
7
Installation and Instrument Setup
8
Installation and Instrument Setup
5. Select the Shaker Only tab to set up mixing parameters (xMark only).
9
Quick Start
Quick Start
Setting Up an Experiment
1. Launch software from the desktop shortcut.
2. Click on the New Experiment icon or choose File > New Experiment to
set up a New Experiment.
10
Quick Start
Open a saved protocol or set up a new protocol (by setting up a template, and
selecting the instrument, assay info, analysis options and print options). Refer to
the
Instrument Controls
1. Click File > Read New Plate. The Instrument Controls dialog displays.
2. Under the Reader tab, set the Reading mode: Endpoint, Kinetic, or
Plate 2-1.
1
Pathlength correction is used to calculate the OD (or % transmission)
for each well, normalized to 1 cm fluid depth (d) in each well.
11
Quick Start
Incubator Controls
Under the Instrument Control dialog box, select the Incubator tab (xMark only)
1. Click the Set to On button.
2. Enter a Temperature Set Point.
3. Click the Monitor Temperature button if you want to monitor the
temperature continuously.
You can also activate the Temperature monitor from the File menu. This window
regularly monitors the xMark temperature status, listing the current temperature &
set temperature and whether the incubator is on or off.
Shaker Controls
In the xMark, the shaker can be set to mixing mode, with no plate read.
12
Quick Start
Reading Microplates
After all Instrument settings have been completed, press the Start Read button to
read the plate.
Data analysis results can be viewed, changed, saved, and printed after raw data are
received from the instrument. See the Analyzing Data
Using a Standard Curve and the Analyzing Data Using Cutoffs sections.
13
Data and Protocol Files
Protocol Data
.pro is the extension for a protocol file. .mpl is the extension for a data file.
When protocol files are defined and When a data file is created, a protocol can be defined
saved, they become an independent file and saved within the assay, or an existing Protocol File
and can be used as a guide for many can be used, modified and saved as part of the Data
assays. file.
14
Data and Protocol Files
The Sample Data files are stored in the following default location:
C: \ Bio-Rad \ Microplate Manager 6 \ Sample Data
The Sample Protocol files are stored in the default location
C: \ Bio-Rad \ Microplate Manager 6 \ Sample Protocols
Newly-generated Protocol and Data files are stored in the default location:
C: \ Bio-Rad \ Microplate Manager 6
Opening Files
To open an existing Protocol File or Data File, click on the Open icon in the Main
tool bar or Select Open from the File Menu on the Main Toolbar. Select the name of
the file you want to open, and click on the Open button.
Saving Files
To save a Protocol File, select Save Protocol or Save As… from the File menu. To
save a Data File, select Save or Save As… from the File menu. If you are saving a
new file or renaming an old file, enter the new file name and click on the Save
button.
The Microplate Manager 6 program opens and saves files from the last-used
directory location.
15
Data and Protocol Files
Data AutoSave
When the AutoSave feature is selected in the Instrument Setup Window, the data
will be saved automatically after each read (and will not be displayed). The file
name will automatically have the date/time and number of the read. This file name
can be changed later.
Importing Data
In order to view data from previous versions,
data must be imported into MPM 6.
1. Open file in Microplate Manager
version 5.2.1 or lower.
2. Choose File > Export "Name of file",
destination, and the suffix ".epc" .
Click Save.
16
Data and Protocol Files
17
Data and Protocol Files
Data can also be entered into the program by typing from the keyboard.
Exporting Data
1. Click on the Export Data icon on the main toolbar and name the file to be
exported. The Export Setup window will appear:
2. Select the type of data and format to be used in exporting and click OK.
18
Data and Protocol Files
Exporting Reports
1. To export data in a report format, including table or matrix headings, select
the desired items such as Info, Plate Data, Standard List, Result List, etc.
from the list in the Content Selector window under the Edit menu.
2. Choose File > Export Report, name and save the report.
An example of an exported standards table from a Percent B/Bo Assay is shown
below:
19
Setting Up a Protocol
20
Setting up a Protocol
7. Once the Template is selected, the program will automatically open Excel
and display the correct Screening Report. The BSE (or other) Screening
Report will be saved automatically with the name that was entered.
The Analysis Settings Worksheet contains settings for control validation and cutoff
criteria.
The cutoff criteria constants, k1, k2, k3 can be changed to modify these cutoff
values. These are the only constants that can be changed in the Workbook. All other
data fields and calculation functions are password-protected and unchangeable.
The Report Worksheet contains the raw test data from the microplate reader. The
Report is a single sheet printable format. It has four sections: the Information
21
Setting Up a Protocol
Section, the Control Validation Section, the Cutoff Values Section, and the Matrix
Data including the Sample info.
In the Information section, the report template, technician, plate, reader, and reading
parameters are given.
The custom report template is uniquely identified with a name, part number and
revision number. Each custom report template is released as a controlled document
with its own part number and revision level. All validation procedures and results are
included with each release.
In the Control Validation Section, the results for the positive and negative controls
are given, as specified in the Analysis Settings Worksheet. The number of valid
controls and the mean absorbance value for each control group (positive and
negative) are shown. These values are highlighted in red, if the control group has
failed validation.
The Calculated Cutoff Values for identifying the positive, negative and indeterminate
samples are shown in the third section. These are calculated as specified in the
Analysis Settings Worksheet.
22
Setting up a Protocol
The matrix of the 96-well absorbance data, including the well identifiers, is shown in
the fourth section. Positive control wells (PC1 and PC2) and negative control wells
(NC1 through NC4) and their absorbance values are shown. Controls that do not pass
validation are flagged in red with strikethrough marks. Each sample well shows the
result (POS, NEG or ???) and its absorbance value. Wells flagged with a *.*** are
out of range, and blank wells are unformatted.
23
Setting Up a Protocol
Setting up a Protocol
Setting up protocols automates your test processes. Parameters for frequently-run
assays can be set up and saved once, and used whenever the assay is performed.
To set up a assay protocol, follow these steps:
1. Set up a Template
2. Select Instrument Setup settings
3. Select Instrument Controls
4. Enter Assay Info
5. Select an Analysis Option
6. Select Report Options
7. Name and Save the Protocol
Sample
Standard
24
Setting up a Protocol
Setting Up A Template
The template defines the identifier locations for your plate data. A template must
always be present in order to see any Result Data or Report window.
The template controls allow you to edit your template, as well as to enter equations
and dilutions. The controls are available as menu items, or as icons under the
Template Window toolbar.
Click on the Template icon in the Main toolbar or choose Template under the
Window menu to open a template.
Blanks, standards or sample identifiers are entered in the template for each well.
Blanks Enter the number sign, # (shift 3) for each blank well. Blank
wells are green with #. The average of all blank wells with
“#” will automatically be subtracted from the raw data.
A second blank can be defined as BLK, if desired, and
equations can be set up to do the subtraction. (see Percent
Control Assay Tutorial on page 82)
To fill in a template, click on a well and enter identifier for that well. To move
from well to well, use the arrow keys or the tab key.
To use the Fill features, highlight a section of the template and select Fill Down,
Fill Right, or Fill Series.
25
Setting Up a Protocol
To highlight the entire plate, choose Select All under the Edit menu. To clear the
template, press the delete key.
To re-size Template columns, in order to display long ID names, position the cursor
in the column number at the top of the window and drag it to the right.
To AutoFill a series of identifiers that begin with a number, highlight the area to
be filled in, click on fill a series icon. In the Fill Series window, enter the desired
concentration or dilution factor. Enter the number of replicates and select the fill
direction. Click OK to fill the template.
Entering Equations
To enter Equations:
click Template View icon. Select Equations.
or choose View > Equations in Main menu.
See the Quantitative ELISA Assay Tutorial section
for more information on how to enter equations.
26
Setting up a Protocol
Entering Dilutions
Dilutions can be entered for any sample well
(except for blanks or standards).
To enter Dilutions:
1. Click the Template View icon.
2. Select Dilutions, or choose View >
Dilutions in the Main menu.
In the Dilution window, the default is all the wells are
filled in with 1.0, indicating that the dilution is one (no dilution).
The dilution factor is the ratio of 1 to the value entered. For 1:2 dilution,
enter the dilution factor as 2. For a 1:100 dilution, enter a factor of 100.
In the Template/Dilutions view, the dilutions appear in the lower part of each well.
27
Setting Up a Protocol
Select up to 6 Custom Labels from a pop-down menu by selecting one of the items in
the list, or by entering a new Label ID in the edit field.
The selected Labels will appear in the Info Window and will be saved in a Protocol.
Custom Labels can also be set up and used for Custom BSE (or other) Screening
Reports.
28
Setting up a Protocol
3. In the Curve Fit Plot Editor, select Auto/ Manual Scaling, Number Format,
Axes labels, etc. for Curve Fit Plot.
29
Setting Up a Protocol
30
Setting up a Protocol
31
Analyzing Data Using a Standard Curve
Analyzing Data
Using a Standard Curve
The standard curve is a plot of the concentration of the standards you have
designated in the template (on the x axis) versus the readings from the instrument (in
OD units for an endpoint experiment (on the y axis).
Microplate Manager 6 provides the following types of curve fit options:
• Linear
• Zero-intercept Linear
• Semi-Log
• Log-Log
• Quadratic
• Log-Logit
• 4-Parameters
• 5-Parameters
• Cubic Spline
• Point-to-Point
To perform a curve fit and display the curve fit
plot on the screen click on the Curve Fit Plot
icon and select the desired curve fit by clicking on the
Choose Fitting Function icon.
If a transformation has been applied to the template, then
the values plotted in the standard curve will be the
transformed values.
The mean value of the replicates of the standards, and not
the individual values, are used in the calculation of the
standard curve.
32
Analyzing Data Using a Standard Curve
Linear Fit
For a linear fit, the best straight line is fitted to the data points, OD's (response) (y-
axis) vs. the standard concentration (x-axis). The equation of the line has the form
y = Intercept + Slope * Conc
The calculated intercept, the slope, the correlation coefficient (r) and chi2 values of
the line are shown below the plot.
33
Analyzing Data Using a Standard Curve
Zero-intercept Linear
In the Zero-intercept of analysis a linear fit is used, but the intercept is set to Zero.
This method of analysis is useful for assays which have a linear response to the
concentration such as protein assays or other assays.
34
Analyzing Data Using a Standard Curve
Semi-log Fit
When Semi-Log is selected in the Curve Fit dialog box, a plot of the response (y
axis) vs. the logarithm of the concentration (x-axis) is obtained as shown below:
35
Analyzing Data Using a Standard Curve
Log/log Fit
The Log/Log curve fit option fits the best straight line to the data consisting of the
logarithm of the OD (response) vs. the logarithm of the concentration.
For the Log/log method, the equation for the standard curve is:
Log10(y) =Intercept + Slope*Log10(conc)
In the plot, the concentration and the OD axes are displayed on the log scale. The
calculated intercept, slope, the correlation coefficient (r) and the chi2 values of the
standard curve are shown below the plot.
The log-log option should be used when a linear plot of the data exhibits a strong
curvature up or down.
Quadratic
The quadratic function fits the best parabolic curve according to the equation
Y = A + Bx + Cx2
where A is the intercept, B is the slope of the curve at the intercept, and C is the
measure of curvature of the parabola.
The quadratic fit is most appropriate when the standard curve curves up or down.
36
Analyzing Data Using a Standard Curve
37
Analyzing Data Using a Standard Curve
The 4-parameter method is usually preferred over the Log/Logit method, because the
results for the 4-parameter method are more accurate.
In both the Log/Logit and the 4-Parameter plots, the concentration axes are displayed
on the log scale. The parameters A, B, C, D, the chi2 values of the standard curve are
shown below the plot.
38
Analyzing Data Using a Standard Curve
Point to Point
In the Point to Point method of analysis, the data points are simply connected. No
fitting of the data points to a line or curve is done.
For reactions where there is a lag time or a plateau, it is often convenient to look at
the data using this plot in order to see the true linear range of the assay.
Cubic Spline
Cubic spline is a piecewise polynomial approximation in which a set of data points
are joined by a series of curve fits. These multiple curve fits are smoothed by using
a cubic fit
y = Ax + Bx2 + Cx3 + D
This type of curve fit is less affected when any one of the standards has a poor value.
Because multiple curve fits are performed with this function, the fit parameters are
not shown with the graph.
39
Analyzing Data Using a Standard Curve
A minimum number of standards that pass certain criteria are required for each type
of curve fit. For the best results, always use more than the minimum number of
standards required to do the calculation.
If these requirements are not met, the Fitting will Fail.
40
Analyzing Data Using a Standard Curve
To obtain a printed standard table report for the imported standard curve, print the
imported standard curve file separately.
41
Analyzing Data Using a Standard Curve
42
Analyzing Data Using a Standard Curve
A residual is the difference between an observed value of the response variable and
the value predicted by the regression line.
That is, Residual = observed y - predicted y.
The chi square value is a measure of how close the observed values are to the
calculated values. A chi value of zero means that the observed values are equal to the
calculated values. Likewise, small chi values indicate a good fit.
chi2 = SQR(Σ(yi-f(xi, p))2/(n-np))
For a set of data points (yi, xi);
y = f(x, p) where p is a set of parameters.
n is total number of values; np is total number of parameters
Correlation coefficient r is a slope of least square regression line for linear plots.
The correlation coefficient, r, is calculated for data pairs (xi, yi) with weighting
factors wi
r = Σ wi(yi-Yi) (xi-Xi) / (Σ wi (yi-Y)2)1/2 (Σ wi (xi-X)2)1/2
X = Σ wixi/Σwi , Y = Σ wiyi/Σwi
43
Analyzing Data Using a Standard Curve
Error of the interpolated concentration (concy) is related to the error of the response
sy according to
s conc = ( δ conc(y)/ δ y) sy
The estimated standard error of concentration (SEMconc) calculated from the mean
response of n replicates is
SEMconc = (δconc(y)/δy) RMS(y) / SQR n
The (δconc(y)/δy) depends on the form of the standard curve.
For a linear standard curve (for example),
Y = A + B* conc → conc(y) = (y-A)/B
(δconc(y)/δy) = 1/B
Wt avg conc = <conc> = Σ wi conci / Σ wi
Weight wi = 1/(SEMi)2
conci interpolated concentration for dilution i
If there are replicate samples with dilution i, conci will be the mean of these
replicates.
SEMi is Standard Error of the Mean for conci.
Example of Weighted Mean
For two concentrations with SEM values as shown,
100 ± 10
150 ± 50
the mean concentration should NOT be taken as 125. The weighted mean of these
two concentrations is 102, since 25 times as much weight is given to the value of 100
with the 5-fold smaller standard error value. When concn is calculated using the
<conc> = Σ wi conci / Σ wi
with wi = 1/(SEMi)2
44
Analyzing Data Using Cutoffs
A template must
always be present in
order to see any Result
Data or Data in the
Table.
45
Analyzing Data Using Cutoffs
The three cutoff display options in the menu are described here. Either two or four
cutoffs are used based on the user definitions in the Cutoffs tab window.
• Gray Scale: In the Gray Scale window, the data values are shown
numerically and in shades of gray. Results equal to or below the lowest
cutoff are white, results equal to or above the highest cutoff are black, and
results between the two cutoffs are shown in shades of gray.
• Cutoff Report: In the Cutoff Report chosen under the View menu, the data
values are shown as --,-, *, or +, ++ if four cutoffs are defined. If two
cutoffs are defined, the results are shown as -, *, or +. Intermediate values
between cutoffs are shown as (*). The report is also displayed in color.
High values are displayed in red, low as blue, and intermediate as white.
• Extended Cutoff Report: In the Extended Cutoff Report chosen in the
View menu, the data values are shown as --,-, 0-9, or +, ++ if four cutoffs
are defined. If two cutoffs are defined, the results are shown as -, 0-9, or +.
Intermediate values between cutoffs are shown as 0-9. The report is also
displayed in color. High values are displayed in red, low as blue, and
intermediate as white.
• + for addition
• - for subtraction
• * for multiplication
• / for division
• Parentheses () may be used.
Numeric constants can be entered in two forms:
• decimal (e.g. -2.51)
• exponential (e.g. -2.51 E3 or 3.8 E-3)
46
Analyzing Data Using Cutoffs
Reference can be made to standard or sample IDs that have been defined in the
Template setup
• Standards: enter ! followed by concentration
• Unknowns: enter identifier
• Blanks: enter #
In all Cutoff equations, Microplate Manager 6.0 uses the averaged value of that
group.
Examples of valid expressions:
• U01
• (U01+.1)/2
• (!100-!10)*2
47
Analyzing Data Using Cutoffs
Identifiers used in
the Cutoff Report
Editor must be used
on the template.
48
Analyzing Data Using Cutoffs
1. To view the results in tabular format, click on the Report icon in the main toolbar.
49
Analyzing Data Using Cutoffs
3. In the Columns Selector window, move Ext. Cutoff Value into the included item.
Move the items up or down to select which items to include in the Report and change
their order. Select Sorted By Mean and Descending order.
50
Analyzing Data Using Cutoffs
The information entered in the Info window is automatically listed at the top of the
Report.
Concentration unit information is displayed on the Tables and Curve fit Plot.
51
Running Kinetic Assays
52
Running Kinetic Assays
3. Enter the interval between the start of two consecutive reads in the Interval
column.
The interval time is the time necessary for one read plus mix time specified.
4. Enter mix time, if desired.
In the above kinetic read example, the reader will do 40 readings with 30 second
intervals and no mix.
5. After the parameters are set, click the Read Plate icon to start the readings.
The raw data are shown in the initial displays as the total run time, beginning from
time 0 to the end of the run time.
53
Running Kinetic Assays
54
Running Kinetic Assays
55
Running Kinetic Assays
After the read is completed, data can be excluded from calculation by defining a new
time range or new OD range. Original data is not lost, but is not included in the
current calculation.
Set time or OD cutoffs on the data, to exclude part of the data from the
analysis. This is useful for excluding an initial lag time in delayed reactions
or for excluding the end of a reaction where the reaction approaches
equilibrium.
For reactions where the OD values decrease with time, the rates will appear
as negative values (negative kinetics). To change the values to positive
numbers, apply the transformation -1*@ to the data.
• Maximum Rate - The Maximum Rate option is useful, as an alternative to
selecting the linear part of the curve using OD and time limits. In the
Maximum Rate method, the OD vs. time curve is segmented and a series of
linear curve fits are performed in order to determine the maximum rate.
The first segment starts at the first data point within the selected time and
OD range, the second segment starts at the second data point, etc. The
maximum rate is determined by evaluating all of these rate calculations.
For cases where the OD values decrease with time (negative kinetics) the
rate values will be negative. To change the sign of negative values, apply
the transformation -1*@ to the data.
56
Producing Reports
57
Producing Reports
Producing Reports
Setting Up Reports
To create a Report, click on the Report icon in the main
toolbar, or select Report under the Window menu.
The Edit and the View menus include the options shown below:
58
Producing Reports
Format Report
Print Preview
In the Contents Selector dialog, move the contents items above or below the “Include
Items Above” line to select items and arrange the order.
1. Click Report icon in Main toolbar.
59
Producing Reports
A section of the Report Standard and Samples Tables with user-defined name
‘%B/Bo” as a column header is shown here.
60
Producing Reports
With Report selected under the Window menu, choose File > Print to print the
report.
Printing Reports
1. Choose File > Page Setup, to set margins and paper size and
orientation.
2. With Report selected under Window menu & Report as the active
window, Choose File > Print to print the report.
61
Producing Reports
62
Appendix
Customizing Reports
1. Click on the Report icon on the main toolbar.
2. Click on the Format Report icon on the report window.
In the Report Format Editor window, the mean response column heading in the
Report can be customized, for Results that have been transformed by equations. For
example, the heading %B/Bo entered here will appear in the report column heading
as “Mean %B/Bo”.
In this window, the Number Format for the report is selected. Scientific number
format will be used in the report tables and plot if this box is checked.
Decimal Position 3 indicates that 3 number digits will be displayed after the decimal.
63
Appendix
Appendix
References
[1] Channing Rodgers, R. P. (1984) "Data Analysis and Quality Control of Assays:
A Practical Primer" in Practical Immunoassay W. R. Butt (ed), Marcel Dekker, NY,
253-308.
[2] Press, William H.; Flannery, Brian P.; Teukolski, Saul A.; Vetterling, William
T. “Numerical Recipes in C: The Art of Scientific Computing”, Cambridge Univ.
Press, NY, 1988.
[3] Davis, S.E., Munson, P.J., Jaffe, M.L. and Rodbard, D. (1980), J. of
Immunoassay, 1, 15. Radioimmunoassay Data Processing.
[4] Finney, D.J. (1976), Biometrics 32, 721. Radioligand Assay.
[5] Rodbard, D. and Lewald, J.E. (1970), Computer analysis of radioligand assay
and radioimmunoassay data. Acta Endo. 64, Suppl. 147, 79.
[6] Rodbard, D. and Hutt, D.M. (1974) "Statistical Analysis of Radioimmunoassays
Assays" in "Radioimmunoassay and Related Procedures in Medicine", Int. Atomic
Energy Agency,Vienna, 165
[7] Rodbard, D., Lenox, R.H., Wray, H.L., and Ramseth, D. (1976), Statistical
Characterization of the Random Errors in the Radioimmunometric Dose Response
Variable. Clin. Chem. 22, 350
[8] Rodbard, D., Munson, P. J., and de Lean, A. (1977) "Improved Curve Fitting,
Parallelism Testing, Characterization of Sensitivity and Specificity, Validation, and
Optimization for Radioligand Assays" in "Radioimmunoassay and Related
Procedures in Medicine", Int. Atomic Energy Agency, Vienna, 469
[9] Malan, P. G., (1982) "Immunoassay Data-processing and Quality Control Error
Analysis in RIA Design", Pergamon Press France, 45-58.
64
Appendix
65
Appendix
66
Appendix
67
Appendix
68
Appendix
69
Appendix
o Standards are defined 200, 100, 50, 25, 12.5, 6.25, 3.125, & 0.
o S01 to S20 are all of the unknown samples to be analyzed.
70
Appendix
3. Highlight area well A3-D3. (Click & hold mouse on well A3, drag
mouse to well D3, and release.)
4. Click on the Fill Series icon in the Template Toolbar
5. Enter as below and click OK
8. Click on well E3
71
Appendix
72
Appendix
73
Appendix
5. In the Curve Fit Plot Editor window under Plot tab, select the Color,
Symbol, and Line style for the Data Points and Curve Fit Line.
74
Appendix
6. In the Curve Fit Plot Editor window under X-axis tab, enter
Concentration for the axis label. Set the Range to Auto.
7. In the Curve Fit Plot Editor window under Y-axis tab, enter
Absorbance for the axis label. Set the Range to Auto.
75
Appendix
8. The customized curve fit plot shows the curve fit parameters below the
curve
76
Appendix
77
Appendix
78
Appendix
79
Appendix
A section of the Data Analysis Report for Standards and Samples Tables with
Average Concentration is shown here.
80
Appendix
7. Click the Print icon in the Print Preview Toolbar to Print the
Report
81
Appendix
82
Appendix
83
Appendix
5. To apply this equation to the data, right click the mouse and select
the % CNTRL equation from the popup.
84
Appendix
85
Appendix
6. The resulting Extended Cutoff Report is shown here with the high
values shown in red, the low values in blue, and the intermediate
values in white:
86
Appendix
87
Appendix
5 Setting up Report
1. Click Report icon in Main Toolbar
88
Appendix
89
Appendix
A section of the Data Analysis Report for Samples with user-defined name % CNTRL
as a column header is shown here.
90
Appendix
9. Click the Print icon in the Print Preview Toolbar to Print the
Report
91
Appendix
92
Appendix
93
Appendix
5. To apply this equation to the data, right click the mouse and select the %
MAX equation from the popup.
94
Appendix
5. In the Curve Fit Plot Editor window under Plot tab, select the Color,
Symbol, and Line style for the Data Points and Curve Fit Line.
95
Appendix
6. In the Curve Fit Plot Editor window under X-axis tab, enter
Concentration for the axis label. Set the Range to Manual with the
minimum 1.0 and the maximum 100000.
96
Appendix
7. In the Curve Fit Plot Editor window under Y-axis tab, enter % B / Bo
for the axis label. Set the range to Manual with the minimum 40.0 and
the maximum 100.00.
97
Appendix
8. The customized curve fit plot shows the curve fit parameters below the
curve.
98
Appendix
99
Appendix
3. Move the items up or down to select which items to include in the Report
and change their order. Select Fitting Info to list the fitting parameters in
the Report.
100
Appendix
101
Appendix
A section of the Data Analysis Report for Standards and Samples Tables with user-
defined name % B/Bo as a column header is shown here.
102
Appendix
9. Click the Print icon in the Print Preview Toolbar to Print the Report
103
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