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Project Digestion of Starch Class 12

This document is a chemistry investigatory project report submitted by a student named Arati Ramdas Wanole. The project examines the digestion of starch by salivary amylase and the effects of temperature and pH on this process. It includes an introduction explaining the role of saliva in digestion. The objectives, materials, procedures, observations and conclusions of 3 experiments are described. The first experiment looks at starch digestion over time, the second examines the impact of temperature, and the third analyzes the impact of pH. The student found that salivary amylase digests starch and that temperature and pH affect this process. Bibliographic sources are listed at the end.

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Shreyash Pol
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764 views

Project Digestion of Starch Class 12

This document is a chemistry investigatory project report submitted by a student named Arati Ramdas Wanole. The project examines the digestion of starch by salivary amylase and the effects of temperature and pH on this process. It includes an introduction explaining the role of saliva in digestion. The objectives, materials, procedures, observations and conclusions of 3 experiments are described. The first experiment looks at starch digestion over time, the second examines the impact of temperature, and the third analyzes the impact of pH. The student found that salivary amylase digests starch and that temperature and pH affect this process. Bibliographic sources are listed at the end.

Uploaded by

Shreyash Pol
Copyright
© © All Rights Reserved
Available Formats
Download as DOC, PDF, TXT or read online on Scribd
You are on page 1/ 15

PM Shri Jawahar

Navodaya Vidyalaya
Kolhapur
CHEMISTRY
Investigatory
Project
2023-2024
Name:Arati Ramdas Wanole
Class: XII
Roll No.:04
NAME OF THE PROJECT:-

To Study the digestion of


starch by salivary amylase
and effect of temperature and
pH on it

CERTIFICATE
This is to certify that this project work in chemistry “To
Study the digestion of starch by salivary amylase and
effect of temperature and pH on it” is undertaken and
completed by Ku Arati Ramdas Wanole of class 12 th
science as prescribed by CBSE for AISSCE,2023-2024.
Date- 20.11.2023
Place: PM SHRI JNV KOLHAPUR

Incharge: G.T. HIRVE


PGT (Chemistry)

Internal Examiner External Examiner

Principal
PM SHRIJAWAHAR NAVODAYA VIDYALAYA
KOLHAPUR
ACKNOWLEDGEMENT

I would like to express a deep sense of thanks & gratitude to my


project guide Mr. G.T,HIRVE PGT CHEMISTRY Sir for guiding me
immensely through the course of the project. He always evinced keen
interest in my work. His constructive advice & constant motivation have
been responsible for the successful completion of this project.

My sincere thanks go to Shri D RAVI DAMODAR, Our principal


sir, for his co-ordination in extending every possible support for the
completion of this project.

I also thanks to my parents for their motivation & support. I must


thanks to my classmates for their timely help & support for compilation
of this project.

Last but not the least; I would like to thank all those who had
Helped directly or indirectly towards the completion of this project.

NAME OF STUDENT:

Class: XII Science


CONTENTS

1. Objectives of the Project. . . . . . . . . . . . .

2. Introduction. . . . . . . . . . . . . . . . . . . . . . .

3. Material Required . . . . . . . . . . . . . . . . . .

4. Procedure. . . . . . . . . . . . . . . . . . . . . . . . .

5. Observation . . . . . . . . . . . . . . . . . . . . . . .

6. CONCLUSION. . . . . . . . . . . . . . . . . . . .

..

7. BIBLIOGRAPHY. . . . . . . . . . . . . . . . . .
...
Objectives of the Project Report

The main objective of this chemistry project


report is “To Study the digestion of starch
by salivary amylase and effect of
temperature and pH on it”and

1) To study digestion of starch by saliva.


2) To study the effect of temperature on the digestion of
starch by saliva.
3) To study the effect of pH on the salivary digestion of
starch.
Introduction
Every Health book insists on the chewing of food. The
act of chewing stimulates the excretion of saliva.
Saliva mixes up with the food and helps its digestion.
That is, the enzyme ptyalin or amylase present in
human saliva hydrolyzes the big molecules of food
into many molecules. For example starch into mono-
saccnaricies maltose and glucose; proteins into amino
acids and fats into fatty acids and glycerol. Thus saliva
not only helps in digestion of food but convert it into
energy generating substances. Further, enzymes and
their activity are very sensitive to temperature and
ph. Even a slight variation in these two factors, can
disturb the action of enzymes. In other words,
digestion of food by salivary amylase is also effected
by pH and temperature and can be verified
experimentally. For example, hydrolysis of starch can
be verified by testing it with iodine solution. Starch
forms blue coloured complex with iodine. If no starch
is present in a system it will not give blue colour with
iodine.
Material Required

The requirements for experiment of Chemistry


Project Report are as under:

1) Test tubes

2) Test tube stand

3) One dropper

4) Beaker

5) Stop watch

6) Starch and Iodine solution

7) Thermometer

8) Dilute HCL and Dilute NaOH solution.


Chemistry Experiment 1 Procedure

Procedure for Chemistry Experiment 1 :

1) Collection of Saliva– Rinse mouth throughly with cold


water and ensure that it does not contain any food
particles. Now take about 20ml of lupe warm water in
the mouth and gangle for about three minutes so that
saliva mixes up well with it. Spit this into a beaker.
Filter, if there is any suspended impurity clear filtrate is
saliva solution and contains enzyme ptyalin.
2) Preparation of starch solution – Take about 0.5g of
starch in 100ml beaker and add enough water to make a
paste. Dilute the paste by adding 50ml water and boil for
about 5 minutes.
3) Digestion of starch :-
(a)Take 5ml of the starch solution in a test tube. Add 2ml
of saliva solution into it. Mix the solutions well by
shaking the tube carefully and start a step watch.
(b) After one minute take out two drops of the mixture
solution from the test tube with the help of a dropper
and transfer it into another test tube containing about
one 1ml of 1% iodine solution. Note the colour
produced, if any.
(c)Repeat this test after every one minute taking two
drops of the mixture solution and fresh 1% iodine
solution continue until the test shows no blue colour.
Record the time and blue colour intensity.
Observation

Time Passed 1 2 3 4
after Mixing Minute Minute Minute Minute
Colour Deep Blue Light No Blue
Intensity Blue Blue

Absence of blue colour on addition to iodine solution


means absence of starch in the mixture solution. That is
whole of the starch has got digested or hydrolysed.
Chemistry Experiment 2

Procedure: -Effect of temperature on the digestion of


starch
by saliva.

1) Take three test tubes and label these 1, 2, and 3.


2) Take 5ml of the starch solution, 2ml of the saliva solution
and 5ml of water in each test tube.
3) Place test tube Number 1 in water at room temperature, test
tube Number 2 in a beaker containing water at 500 C and
test tube Number 3 in boiling water.
4) After 5 minutes, observe the colour change on mixing two
drops of the mixture of every tube with one ml of 1%
iodine solution. Note the intensity of blue coloured form.

Conclusion

Starch gets hydrolysed by saliva amylase


Chemistry Experiment 3

Procedure: - To study the effect of pH on the salivary


digestion of starch

1) Take three test tubes and label these 1, 2, and 3.


2) Add 5ml of the starch solution, 2ml of the saliva
solution in each test tube.
3) Now ass 2ml of water in test tube Number 1 2ml of
Dilute HCL in test tube Number 2 and 2ml of Dilute
NaOH solution in test tube Number 3 and shake
carefully.
4) Keep the three test tubes in water at room temperature
for about 10 minutes.
5) Add 2 drops of the solution of each test tube with 1%
iodine solution and observe the colour change

Conclusion

Temperature effects the digestion of starch by salivary


with increase in temperature salivary amylase get
inactivated and process of digestion do not take place.
BIBLIOGRAPHY
1 http://www.google.com/

2 http://en.wikipedia.org

3 N.C.E.R.T Class 12
Chemistry

4 Dinesh Chemistry

5 Comprehensive Chemistry

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