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Plant Disinfection Using 70 Percent Ethanol and 0.1 Percent Bleachsolution

This document provides guidance on disinfecting surfaces and equipment in a laboratory setting using 70% ethanol or 0.1% sodium hypochlorite (bleach). It describes the materials needed, shelf lives, safe handling procedures, hazards posed, necessary personal protective equipment, spill response procedures, and accident reporting requirements for both disinfectants. Procedural steps are outlined for preparing the solutions and applying them, including recommended contact times.

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Mai Thanh Thao B1904601
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14 views

Plant Disinfection Using 70 Percent Ethanol and 0.1 Percent Bleachsolution

This document provides guidance on disinfecting surfaces and equipment in a laboratory setting using 70% ethanol or 0.1% sodium hypochlorite (bleach). It describes the materials needed, shelf lives, safe handling procedures, hazards posed, necessary personal protective equipment, spill response procedures, and accident reporting requirements for both disinfectants. Procedural steps are outlined for preparing the solutions and applying them, including recommended contact times.

Uploaded by

Mai Thanh Thao B1904601
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
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Disinfection using 70%Ethanol and 0.

1%Sodium Hypochlorite (Bleach)

Title or Type of Procedure: Use of disinfectants for routine laboratory


decontamination of surfaces and equipment.

P. I. Lab Location:

Original Issue Date: Revision Dates:

Prepared By: Approval Signature:


(if required by lab supervisor)

Procedural Methods and Materials:

Procedural Methods and Materials:


70% Ethanol; shelf life ~6 Months
Alcohols are volatile and flammable and must not be used near open flames.
Working solutions should be stored in closed containers to avoid evaporation.
Bottles with alcohol-containing solutions must be clearly labeled to avoid autoclaving.
Alcohols can be used on skin, work surfaces of laboratory benches.

To prepare 1 liter of 70% Ethanol using 95% lab Ethanol add 740ml of lab ethanol to
260ml water.

0.1% Bleach; shelf life ~30 days


Sodium hypochlorite (bleach) solutions are unstable. When open to the air, chlorine
evaporates at a high rate from the solution, rapidly reducing the concentration of free
chlorine. When stored at room temperature and away from sunlight, bleach loses 20-50%
of its sodium hypochlorite concentration after 6 months; breaking down into salt and
water. Bleach solution is corrosive to stainless steel; therefore, thorough rinsing must
follow its use.

To prepare 1 liter of 0.1% Sodium hypochlorite using commercial bleach add 20ml of
commercial bleach to 880 ml water.

Procedure: Apply disinfectant to contaminated or potentially contaminated area. If


visible or gross contamination is present, apply enough disinfectant to saturate the
contamination. Let stand for the indicated contact time (minimum 1 minute) If using
bleach rinse with water after use.

Hazard Identification and Risk of Exposure to the Hazards:


1.Creation of infectious aerosols
2.Exposure to respiratory and eye irritants

3.Always keep ethanol solutions away from potential sources of ignition. Prolonged
and repeated use of alcohol as a disinfectant can also cause discoloration, swelling,
hardening and cracking of rubber and certain plastics.

4.Chlorine solutions should never be mixed or stored with cleaning products containing
ammonia, ammonium chloride, or phosphoric acid. Combining these chemicals will
result in the release of a chlorine gas, which can cause nausea, eye irritation, tearing,
headache, and shortness of breath.

Exposure Controls Specific to Above Risk of Exposure:


The PPE to be worn when working with disinfectants should be commensurate with
the highest risk or hazard designation for any single biological agent or material
present prior to disinfection.
• Eye and respiratory protection should used whenever the creation of aerosols is
possible
Bring SDS to area where seeking medical attention.

Waste Generated and Disposal Methods:

Spill Response Procedures:


Contain spills if possible.
Accident Response Procedures:
• Call 911 or seek medical attention.
• Report the incident to your supervisor as soon as possible, fill out the appropriate
documentation.
Note: It is important to fill out all of the appropriate documents to be eligible to collect
workers compensation should any complications from the hazardous exposure arise
in the future.

Notes: (special record keeping such as inventories for toxins, reporting, training, etc.
that may be required)

References:
For Material Safety Data Sheets:
http://www.sigmaaldrich.com/safety-center.html
https://www.fishersci.ca/ca/en/catalog/search/sdshome.html

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