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Developing A GPC Processing Method Empower

This document discusses developing a gel permeation chromatography (GPC) processing method. It explains taking GPC data into the Review window, using the Processing Method Wizard to develop a method which can then be added to a method set. Key steps include integrating and calibrating the data, setting the run time, and viewing noise and drift results to ensure suitable system performance. The summary emphasizes building the processing method with the highest molecular weight standard and testing it on the full range of sample molecular weights.

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Theodora Seviani Amelia
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184 views

Developing A GPC Processing Method Empower

This document discusses developing a gel permeation chromatography (GPC) processing method. It explains taking GPC data into the Review window, using the Processing Method Wizard to develop a method which can then be added to a method set. Key steps include integrating and calibrating the data, setting the run time, and viewing noise and drift results to ensure suitable system performance. The summary emphasizes building the processing method with the highest molecular weight standard and testing it on the full range of sample molecular weights.

Uploaded by

Theodora Seviani Amelia
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
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Chapter Two

Developing a GPC Processing Method


After completing this section you should be able
to do the following:
◆ Take GPC data into Review
◆ Make changes to the Review window and Save
Preferences
◆ Develop a GPC processing method with the wizard
◆ Understand System Suitability
◆ Add the processing method to a method set
Zoom function

2D Channels Tab
Note position of SampleName
Processing Method Wizard
Processing Method Name
Integrate Calibrate Next 2D Channel
These tabs will only be available if you
have the System Suitability option
Calculating Baseline Noise and Drift

Run Time=10 minutes


Viewing Noise & Drift Results

The Results Table in Review


Method Set tool

Processing Method Name


Method Set Name
Summary

Processing methods are developed in the


Review window.
Always build the processing method with the
highest molecular weight standard.
Use the Processing Method Wizard or develop
a processing method manually.
Use integration events to “fine-tune” your
method.
Test the method on the range of molecular
weights.

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