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Dimethyl sulfide (DMS) is a polar, aprotic organic solvent that is commonly used as a cryoprotectant to prevent freezing damage to tissues. DMS reduces ice formation and cell death during freezing by preventing crystallization. Cell viability assays, such as the resazurin assay and MTT assay, are commonly used to investigate cell response to drugs and determine if cells are alive or dead. The MTT assay depends on the ability of NADH-dependent oxidoreductases in living cells to reduce the tetrazolium salt MTT to form an insoluble purple formazan product. This allows quantification of viable cells based on the amount of formazan produced.

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18 views

Untitled Notebook

Dimethyl sulfide (DMS) is a polar, aprotic organic solvent that is commonly used as a cryoprotectant to prevent freezing damage to tissues. DMS reduces ice formation and cell death during freezing by preventing crystallization. Cell viability assays, such as the resazurin assay and MTT assay, are commonly used to investigate cell response to drugs and determine if cells are alive or dead. The MTT assay depends on the ability of NADH-dependent oxidoreductases in living cells to reduce the tetrazolium salt MTT to form an insoluble purple formazan product. This allows quantification of viable cells based on the amount of formazan produced.

Uploaded by

y77gvccyfr
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as PDF, TXT or read online on Scribd
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17 MSO "Dimethyl Sulfide"

·
polar, aprotic organic solvent... has no
hydrogen bond + don't protones.
--

·
Connonly used as
Cryoprotectant - Save tissues from freezing damage.
why?:

it reduce ice formation and cell death


during freezing.
prevent
Crystleing.

IC 50
drug dose:

inhibit functional.

Cell
viability assay:
Cell Liter bene "Resazurin Assay"
important for determain Cells are alive or dead.

lowernightl
· blue
- -

Commonly used to investigate cell


response to the
drug.
How it works?:

Alive to mito produce NADH/ATP


.
sjsi side is 8.35! ie:
-i sI
gig;
·
Egoss! So I s0!-[* Dehydrogenases, NADH S

S
= -,

The MTTassay

Colormetric assay for Cell


Viability;
· Depend on the ability of NADH dependentoxidoreductase
to reduce tetrazolium (MIT) to insulible purples

principle:
MTTdimethysthol diphenyletetrazulum bromide

Co ↳ Tetrazotum
can be
salt

reduced to
-
yellow
formazan --
purple
=

.
s-s1g ?H

--

Isis

* Cytostatic activity - I
I s
-51 -s-125 is Xisi. i 5.

↑TT
lights, (pps jeeps"S?S(MTT
- 5199..
.
: s.. DMS0 Dis. 35-2s j: :

- is 50s -
"s

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