SAMPLE QUESTION PAPER (TERM |) 2021-22 CLASS Xi BIOTECHNOLOGY -045, Time: 90 Minutes General Instructions: 1. The Ques on Paper contains three sec ‘ons. 2. Sec on Aas 24 ques ons. A empt any 20 ques ons. 3. Sec on Bhas 24 ques ons. A empt any 20 ques ons. 4-Sec on Chas 12 ques ons. A empt any 10 ques ons, 5. All|ques ons carry equal marks. 6. There is no nega ve marking. ‘SECTION - A Section — A consists of 24 questions. Attempt any 20 questions from this section. ‘The first attempted 20 questions would be evaluated. 1. itis possibie to introduce colours into DNA by A. FISH B. Microaaray &. Nick translation D. SNP ‘The protein that provides the body structure and protection to our bones Is P< collagen. B. hemoglobin C. actin D. myosin 3. _ | Restriction enzymes were discovered by AC W. Arber, H. Smith and D. Nathans B. Paul Berg and Herbert Boyer C. Annie Chang and Stanley Cohen D. Kerry Mullis 4. _ | Foreign DNA is directly introduced in to the recipient call using a fine mioro-syringe to transform it in the following technique- A. Electroporation 8” Microinjection C. Biolistics D. TransfeWhich Teature ofthe vectors provides Nexibliy nthe choice of restrcion enzymes? ——~ A. MCs. B. Ori C. Unique restriction enzyme recognition site D. Size Interferon B is used for the Weatment of A. Hepatitis C B. Hepatitis B C. Multiple Sclerosis D. Chronic Granulomatous disease {In-situ activation of chymotrypsin takes place in the A. jejunum. B. duodenum. C. ileum. D. pancreas. ‘The enzymatic activity of subiilisin is contributed by A. Ser 221, His 64 and Asp 32 B. Ser 32, His 221 and Asp 64 C. Ser 221, His 32 and Asp 64 D. Ser 32, His 64 and Asp 221 The most common type of restriction enzymes used in recombinant DNA technology are A. Type I restriction enzymes B. Type Il restriction enzymes C. Type lll restriction enzymes D. Type IV restriction enzymes 10. “Aifern cycles, the number of DNA copies produced are- An B. 2" C. x2 D. +2 1 Tosmids have the cos site of A. BAC B. YAC cc. Plasmid D. Lambda 12. Following enzyme prevents self ligation DNA ligase Eco RI DNA polymerase Alkaline Phosphatase pop>13 14 15 Essential amino acids are those which ‘A. have to be obtained from food B, can be made in our cells C. can not be obtained from food D. need not be essentially supplemented in the diet | Severe combined immunodeficiency is caused due to the absence of A. prions. B. chymotrypsin. C. esterases. D. adenosine deaminase. Culture based approaches for detecting pathogens, as compared to PCR based assays are A. faster, safer but less specific. B. slower but safer and more specific. C. slower, less safer and less speci D. slower, less safer but more specific. 16 100 Kb DNA fragment has to be cloned in a host cell. Which vector should be used for this experiment? A. Plasmid B. Cosmid Cc. BAC D. Bacteriophage lambda 17 The tripeptide present in whey that makes it a nutraceutical protein is A. Glutathione B. Kappa -casein C. Hexokinase D. Urease 18 Different proteins move differently in SDS -PAGE. It is due to varying A. size B. charge C. isoelectric point D. pH 19 The Bioactive molecule used to treat Myocardial Infarction is a A. proteolytic enzyme. B. monoclonal antibody. C. non-catalytic protein. D. polyclonal antibody.20 Ina POR reaction folowing components were taken- ds DNA. Taq polymerase, NTPs and ds primer, but even after 30 cycles, no amplification of the target DNA could be seen. The reason for this is because the ‘A. template is DNA and not RNA. B. ddNTPs are not added in the reaction mixture. C. primer is double stranded. D. reverse transcriptase is not added. 24 In Protein fingerprinting technique A. paper chromatography is followed by paper electrophoresis. B._ paper electrophoresis is followed by paper chromatography. . paper chromatography and paper electrophoresis are carried out simultaneously. D. neither paper chromatography nor paper electrophoresis takes place 22 VW ho has received Nobel prize for deducing the structure of haemoglobin? A. Linus Pauling B. Max Perutz C. V.M Ingram D. Frederick Sanger 23 Which functional property of whey proteins, where they undergo maillard reaction, is used in confectionaries? A. Viscosity B. Gelation C. Browning D. Emulsification 24 Name the dye which is used for the visualization of Proteins in 2D gel electrophoresis? A. Ethidium bromide FDA Silver stain Evan's blue :on SECTION -B Section - B consists of 24 questions (SI. No.25 to 48). Attempt any 20 questions from this section, The first attempted 20 questions would be evaluated. 25 Why are eukaryotic hosts preferred for expressing eukaryotic recombinant proteins? A. Enzymes necessary for the proper folding of the proteins are absent in eukaryotes. B. Enzymes necessary for the splicing mechanism are absent in eukaryotes. : C. Machinery for removal of introns is not there in the prokaryotes. : : Enzymes necessary for post-translational modi eae il modifications are also absent in MU| D. Protein fingerprinting 37 th goal of mass spectrometric analysis is to create ‘A. gas phase ions from polar charged molecules. B. polar charged molecules from gas phaso ions. C. liquid ions from non-polar molecules. D. non-polar molecules from liquid ions Which technique is used for improving laundry detergent subtilisin? A. SDS-PAGE B. Mass Spectrometry C. Site directed Mutagenesis inthe enzyme chymotrypsin, why does Ser 195 develop a negative charge on its -OH group? ‘A. Negatively charged Asp COO residue pulls the Ser-OH proton through His B. Negatively charged Ser COO’ residue pulls the Asp-OH proton through His C. Positively charged Asp COO residue pulls the Ser-OH proton through His D. Positively charged Ser COO’ residue pulls the Asp-OH proton through His 40 Chymosin and Papain are industrial enzymes used respectively in ‘A. cheese and beverage industries. B. beverage and cheese industries. . soap and cheese industries. D. leather processing and meat industries, a Which one of the following is an autosomal recessive disorder? A. Cystic Fibrosis B. Huntington disease C. Alzheimer's disease D. Migraine‘Question No. 42 to 44 consist of two statements ~ questions selecting the appropriate option given Aasertion (A) and Reason (R). Answer these below: 4 ue and the reason is the correct explanation of the A. Both Assertion and Reason are t assertion ae B. Both Assertion and Reason are true but the reason isnot the correct explanation o assertion CC. Assertion is true but Reason are false D. Both Assertion and Reason are false 2 | Assertion: Human diet should compulsorily contain glycine, serine and tyrosine Reason: Essential amino acids can be synthesized in the human body. 3] Assertion: Kappa casein is involved in micelle stabilization of mik proteins and keeps the proteins suspended uniformly in milk Reason: Kappa casein behaves like a lipid molecule and 2/3 rd of milk protein is hydrophobic. 44 | Assertion: At isoelectric point, the amino acid does not migrate under the influence of electrical field, Reason: The amino acid molecule at the isoelectric point exists as zwitter ion structure. 45 ‘Which chromosome changes to Philadelphia chromosome due to reciprocal translocation? A. chromosome 9 B. chromosome 22 C. Either A or B D. chromosome 24 46 Physicians decide our susceptibility or resistance to a particular disease through- A. SNPs B. Nick translation C. FISH D. SDS-PAGE 47 Whey has been administered to the sick for the treatment of numerous ailments as it results elevation of a tripeptide glutathione in cells. reduction of a tripeptide glutathione in cells. reduction of xenobiotic levels in our body. elevation of xenobiotic levels in our body. pomp48 Section- C consists of one case followed by 6 questions linked to t ; ic Besides this, 6 more questions are given. Attempt any 10 questions in this section. attempted 10 questions would be evaluated. Biological Value refers to A 2. {he amount of protein nitrogen that is retained by the body from a given amount of Protein nitrogen that has been consumed. oon expressed in terms of weight gain of an adult by consuming 1.gm of food ein. B. 9 the total amount of protein nitrogen that has been consumed. growth expressed in terms of weight gain of an adult by consuming 1 Kg of food protein, 9 SECTION - C case (Q.No. 49 to 54). The first Case Gene prediction and counting Gene prediction is an important problem for computational biology and there are various algorithms that do gene prediction using known genes as a training data set. The following table shows Genome size and gene predictions between several organisms. Organism No.of | Genome size | The Number of | Part ofthe ‘hromo|inbase pars | Predicted | genome somes senes that ‘encodes for protein ficiera Escrerincoti |_| 300000 | 5000 90% rest Scchareces 16 | 12,068,000 | 0 08 ceresioe neem Comoros legos] g | 00000000 1 5 an0 on Fy Dropiiomelenosaer | 4 | M5.000.000- Toop se 196,00,000 eed wea AC 15 Ton. | 8 we onan tomo seniens 73 |3,000,00000] 2,000 25, 00] «sx Based on your observation and analysis, answer the following questions. 49 Even if we know where the genes are in a given gen : Ape genome, it's difficult to count them due to B. overlapping genes C. exons D. Both Aand B 50 Which organism has the maximum part of the genome codi Escherichia coll oan Saccharomyces cerevisiae Caenorhabaltis elegans 19 for the proteins? pop> Drosophila melanogaster51 Part of the genome that encodes for protein in Homo sapiens is less than 5 % , one of the probable reason/s for this could be A. Repeated Sequence B. Exons C. Both “a” and" b” D. SNP's 52 The relationship between number of chromosomes and genome size in base pairs is A. direct B. indirect C. no relationship D. correlation of 0.5 53 Computational Gene prediction is referred to as. A. In-silico Gene prediction B. In-Vivo Gene prediction C. In-vitro Gene prediction D. Microarray prediction 55. After observing the table, it seems that the relationship between the intuitive complexity of an organism and the number of genes in its genome is A. No simple correlation B. Simple correlation C. Inverse correlation D. . Depending on the organism, can be simple or Inverse Tie STS PACT RT HTT TTT TOT yoo cane (A) Oneal anne en (@ 8° >. ry In the given figure , DNA fragment is cleaved using enzyme “A”. Identify this enzyme. ‘A. DNA ligase B. EcoRI C. DNA polymerase D. Alkaline phosphatase 10DNA fragment Closing vector (plasmid) (A, scat wth enzyme Bon! je cotwtonenayme (A) I. Ligate together NA ne conten at Recombinant plasmid) Introduce plasmid into E.coli host cell (transformation) chromosome =i aa a ec, ooo Xe . Col cells containing cloned plasmids Schematic representation of the basic steps in ROT. In the given figure , DNA fragment is cleaved using enzyme “A” . Identify this enzyme. A. DNA ligase B. EcoRI C. DNA polymerase D. Alkaline phosphatase——— a, SEO PCR technique Identify the short, single-stranded DNA sequence (A) which anneal to the separated strands of DNA. Primers B. Template C. Taq polymerase D. dNTPs >B. Template C. Taq polymerase D. dNTPs Reading a sequencing get pattern (lines drawn for clarity in reading). The sequence of original strand in the given autoradiogram is- 5’ ACTAGCGATG 3' 5’ CATCGCTAGT 3’ 3’ ACTAGCGATG 5° |. 3’ CATCGCTAGT 5’ oND>y Zz a ‘Y,3" dideory mucleotide triphosphate Deaxy and dideoxy nucleotides. ‘Z’ is an atom/group of atoms in the structure of dideoxynucleotide. Identify this. A. Hydrogen B. hydroxyl group C. carboxyl group D. phosphate groupv. carboxyl group D. phosphate group 7 © @ fe svectener co sreutepn erento 4| = aa parm 2) aa aa =o no fea vet eS The terminals “A” and “B “ as marked in Agarose gel electrophoresis ( RFLP technique) are A. Cathode and Anode respectively B. Anode and Cathode respectively C. Cathode and Neutral charge respectively. D. Anode and Neutral charge respectively.— | | Fragment 8 ‘Cut with EcoRt OK mx a Recombinant ONA malecute Construction of rDNA using fragments from different sources. The label “2” is a type of end produced in a DNA molecule. Identify it? A. Cohesive ends B. Sticky ends C. Blunt ends D. Restricted endsQuestions in lieu of diagram based questions for VI candidates Total Alternative Questions - 07 Section - B 36 The ae ee circular vector with antibiotic resistant genes as selectable markers is: pl PBR 322 BAC YAC gem Section - C 55. In the formation of r DNA molecule , the enzyme which is used to cleave the Gene of interest and vector is A. DNA ligase B. EcoRI C. DNA polymerase D. Alkaline phosphatase 56. The short, single-stranded oligonucleotide DNA sequence which anneal to the 3' region of the denatured strands of DNA. A. Primers B, Template C. Taq polymerase D._ dNTPs 57. Tnvan autoradiogram, we read the DNA sequence as 3° GTAGCGATCA 5'.Trace the sequence of original strand in the autoradiogram = ‘A. 5’ ACTAGCGATG 3° B. 5'CATCGCTAGT 3° C. 3’ ACTAGCGATG 5° D. 3° CATCGCTAGT 5° 58. Diieaxynucleotde differs from deoxynucleotide in lacking an atom/group of atoms which is hydrogen hydroxyl group carboxyl group phosphate group com> 59. The movement of RFLP in Agarose gel electrophoresis is from Cathode to Anode. Anode to Cathode. Cathode to Neutral charge. g9OR> Anode to Neutral charge.Go. ] The ends which are produced afer digesting the genomic DNA with EcoRI ara A, Cohesive ends B. Sticky ends C. Blunt ends D. Restricted ends