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Folins McCarthy Sullivan Test

The Folin-McCarthy-Sullivan test is a biochemical test that detects the amino acid methionine or methionine-containing proteins through a color reaction. When the Sullivan and McCarthy reagent of nitroprusside and alkaline solution is mixed with a methionine sample under acidification, a red color develops, indicating a positive result. This test is efficient for methionine detection as it is quick and can detect methionine even in fermented extracts. It has high specificity for methionine and can differentiate it from other sulfur-containing amino acids. The test was developed in 1941 to detect tryptophan and histidine.

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173 views7 pages

Folins McCarthy Sullivan Test

The Folin-McCarthy-Sullivan test is a biochemical test that detects the amino acid methionine or methionine-containing proteins through a color reaction. When the Sullivan and McCarthy reagent of nitroprusside and alkaline solution is mixed with a methionine sample under acidification, a red color develops, indicating a positive result. This test is efficient for methionine detection as it is quick and can detect methionine even in fermented extracts. It has high specificity for methionine and can differentiate it from other sulfur-containing amino acids. The test was developed in 1941 to detect tryptophan and histidine.

Uploaded by

jeannikkav
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26C NICE CLOUD 10:00 A.

M
08/18/2023
SCOPE OF THE PRESENTATION

THE TOPiC
THEORETiCAl ImPORTANCE
ANd ITS TRiviAS!
RESulT OF THE TEST
PRiNCiPlE

10:00 A.M
26C NICE CLOUD
08/18/2023
FmS TEST
FmS TEST
The Sullivan and McCarthy’s test
This test is a biochemical test for the reagent is the nitroprusside and
detection of the amino acid, methionine, alkaline solution of methionine
or methionine-containing proteins. under acidification and serves
as the basis for such test. It is a
efficient approach for
FmS TEST methionine tracing as it is a quick
A color reaction of proteins which and simple way to assay the
determines the presence of a certain amino amino acid even in fermented
acid by the development of a particular extract.
color with a specific reagent.

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10:08 A.M
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THEORETICAl RESulT

POSITIVE RESULT: The appearance of a


red color yield in the test tube confirms the
presence of methionine.

NEGATIVE RESULT: The negative result is


represented by the absence of the red
color. Thus, infering that no trace of
methionine is found in the sample.

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02 03
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ImPORTANCE
Folin’s McCarthy Sullivan test is significant
simply because it detects the amino acid
2 1 ImPORTANCE
It has a high-degree of specificity for methionine.
methionine or proteins that contain methionine.

ImPORTANCE
It is also utilized to differentiate methionine from
other sulfur-containing amino acids such as
cysteine and cystine.
3

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02 03 04
10:14 A.M
26C NICE CLOUD
08/18/2023
dYK? 01 02

In 1941, this colorimetric method The Folin’s McCarthy Sullivan


was developed when it was Test might give a positive
discovered that Tryptohan (Trp) result with commercial
and Histidine (HIs) both produce leucine as it might contain
a red color with nitroprusside some traces of methionine.
under similar conditions.

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10:16 A.M
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10:20 A.M
26C NICE CLOUD
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