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Lab Worksheet 2

The document discusses equipment used in microbiology and parasitology laboratories. It provides examples of various pieces of equipment like autoclaves, incubators, centrifuges, water baths, inoculating loops and needles, inoculating hoods, alcohol lamps, test tubes and racks, petri dishes, Erlenmeyer flasks, glass slides and cover slips, hot air ovens, beakers, and fermentation tubes. It explains the functions and uses of these pieces of equipment. It emphasizes the importance of familiarity with laboratory equipment for safe and accurate experimentation.

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norpaidaarajalon33
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97 views

Lab Worksheet 2

The document discusses equipment used in microbiology and parasitology laboratories. It provides examples of various pieces of equipment like autoclaves, incubators, centrifuges, water baths, inoculating loops and needles, inoculating hoods, alcohol lamps, test tubes and racks, petri dishes, Erlenmeyer flasks, glass slides and cover slips, hot air ovens, beakers, and fermentation tubes. It explains the functions and uses of these pieces of equipment. It emphasizes the importance of familiarity with laboratory equipment for safe and accurate experimentation.

Uploaded by

norpaidaarajalon33
Copyright
© © All Rights Reserved
Available Formats
Download as PDF, TXT or read online on Scribd
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ST.

BERNADETTE OF LOURDES COLLEGE


COLLEGE OF NURSING
MICROBIOLOGY AND PARASITOLOGY
LABORATORY WORKSHEET
#2
EQUIPMENT USED IN THE MICROBIOLOGY
AND PARASITOLOGY LABORATORY

Name: JUNAID, KATRINA L. Score: __________________


Year & Sec: 3RD YR/ SECOND COURSER Date: ___________________

INTRODUCTION

Microbiology labs are the workhouses for studies about microscopic forms of life. For students, a well-equipped
microbiology lab provides exposure to the equipment that enables the study of microorganisms like microbial morphology,
molecular biology, biochemistry, etc. It also provides a platform for them to increase dexterity in daily lab handling and
helps them hone their microbiology lab skills.

Instruments are essential to hasten and to make work more efficient. Low knowledge of equipment may lead to
wrong use that may lead to erroneous results. In microbiological laboratory, various instruments are used for varied tasks.
They may be under heating instruments like an incubator, a water bath, or an alcohol lamp, while others are used for storage
like a refrigerator. Well equipped with these knowledge is essential not only to the quality of results obtained in
experimentation but also for the safety of the ones using these equipments.

A. Describe the functions and uses of each equipment and illustrate them using the table below

EQUIPMENT FUNCTION ILLUSTRATION


Autoclave Operate at high temperature and
pressure in order to kill
microorganisms ans spores. They
are used to decontaminate
certain biological waste and
sterilize media, instruments and
lab ware.
Bacterial Incubator Used in microbiology labs to
maintain optimal conditions for
the growth and reproduction of
microorganisms. They provide a
controlled environment for
experiments by regulating
temperature, humidity, and CO2
levels.

Centrifuge Separate heterogenous mixtures


into their various components

Water bath Used during incubation in


microbiological laboratory work
because water can retain heat so
well, but can also enable
chemical reactions to occur once
the water reaches a certain
temperature.

Inoculating loops and needles Used for inoculation, serial


dilution, sterile sampling,
transfer and microbiological
sample spreading.

Inoculating hood Used to create bacteria, fungal


free atmosphere in the chamber
with ultra violet germicidal light
and it is used in bio logical
culture studies.
Alcohol lamp Used for heating, sterilization,
and combustion in a laboratory.
The alcohol lamp uses ethyl
alcohol or spirit as a fuel. It is
generally made up of glass, brass
or aluminum.

Test tubes and test tube rack Used when various different
solutions are needed to work
with simultaneously, for safety
reasons, for safe storage of test
tubes. And to ease the transport
of multiple tubes.

Petri dish Used to culture different types of


cells, including bacteria and
molds. It often contains a
nutritional medium on which the
cells can grow. A petri dish is a
flat, shallow dish made of glass
or plastic with suitable lid.

Erlenmeyer flask used to contain liquids and for


mixing, heating, cooling,
incubation, filtration, storage,
and other liquid-handling
processes. Their slanted sides
and narrow necks allow the
contents to be mixed by
swirling without the risk of
spills, which is useful for
titrations and for boiling
liquids.
Glass slides and cover slips Used to mount, or place
specimens in a way that is easier
to handle and that protects them
from cross-contamination.

Hot air oven Sterilizes the product by keeping


them in an incubator at a
particular temperature for few
hours. It will effectively destroy
microorganisms and bacteria
present in the product along
with their spores to keep them
free from contamination.

Beakers useful as a reaction container


or to hold liquid or solid
samples. They are also used to
catch liquids from titrations
and filtrates from filtering
operations.

Fermentation tubes used to verify gas production in


fermentation exercises, with
gas produced by yeast or other
organisms rising into the
evolution tube, and they are
available in graduated or
ungraduated formats, with or
without a foot.
RESULTS AND DISCUSSION

1. Why is it important to be familiar with the different equipment/apparatus used in the microbiology and
parasitology laboratory?

Always know the equipment well before do any type of experiment because without the proper knowledge of your
equipment you will not know how to use your materials or how to correct a mistake that you could make with your
equipment, Knowing one’s science equipment will only aid having a successful experiment and might help in
correcting errors.

2. What is the principle behind the use of an autoclave?

To subject the bacteria to a very high temperature of steam, which can be heated to a much greater temperature than
the normal boiling point of water under pressure.

3. What temperature is used for the autoclave and why is this used?

Autoclaves use saturated steam under pressure of approximately 15 pounds per square inch to achieve a chamber
temperature of at least 250°F ( 121°C) for a prescribed time usually 30-60minutes.

4. Working the microbiology laboratory involves potential risks like exposure to an infectious microorganisms and
hazardous chemicals. How could this be avoided?

Wash hands following all laboratory activities, following the removal of gloves and immediately following contact
with infectious materials. Decontaminate work surfaces before and after use, and immediately after spills. Do not eat,
drink , store food or smoke in the laboratory.

5. What are the important personal protective equipment (PPE) that must always be used in the microbiology
laboratory?

A lab-coat, closed-toe shoes, eye protection( When necessary) and protective disposable gloves.

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