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Data Collection Plan

This document summarizes a study that aimed to test the effectiveness of a hand sanitizer made from natural ingredients like lemon and aloe vera. It describes the various stages of the research process, including determining the problem, gathering materials through extraction of essential oils, preparing experimental setups, testing the sanitizer on moldy bread, collecting and analyzing data, and drawing conclusions. The results showed the natural sanitizer was as effective as commercial ones in preventing mold growth, suggesting its ingredients have antimicrobial properties and it could be a safer alternative to chemical-based sanitizers.

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21 views

Data Collection Plan

This document summarizes a study that aimed to test the effectiveness of a hand sanitizer made from natural ingredients like lemon and aloe vera. It describes the various stages of the research process, including determining the problem, gathering materials through extraction of essential oils, preparing experimental setups, testing the sanitizer on moldy bread, collecting and analyzing data, and drawing conclusions. The results showed the natural sanitizer was as effective as commercial ones in preventing mold growth, suggesting its ingredients have antimicrobial properties and it could be a safer alternative to chemical-based sanitizers.

Uploaded by

sweetzeleve16
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
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Introduction: In our visual illustration of the experimental setup, we aimed to demonstrate the

effectiveness of two natural ingredients, Citrus limon (lemon) and Aloe vera, in creating a potent hand
sanitizer. The setup involved gathering fresh lemons and Aloe vera plants, extracting their essential
oils or juices, and combining them with other necessary ingredients such as, Tea Tree Oil, Xanthan
Gum or Cornstarch, Distilled Water and glycerin. Our objective was to visually capture the process of
creating a hand sanitizer that harnesses the potential antimicrobial properties of these natural
ingredients, potentially providing a safer, chemical-free alternative to commercial hand sanitizers.

Stage 1
Determining the research problem:
In this experimental study titled 'The Potency of Lemon (Citrus Limon) and Aloe Vera as Hand
Sanitizer,' our research problem centers on assessing the effectiveness and attributes of natural
ingredients, Lemon (Citrus Limon) and Aloe Vera, as potential alternatives to conventional hand
sanitizers. Specifically, we seek to understand how the unique properties of these natural
components contribute to their efficacy in hand sanitization, compare their performance against
commercial products in terms of antimicrobial capabilities, user preference, and environmental
impact, thus addressing critical questions surrounding their viability as safe and sustainable options
for hand hygiene.

Stage 2
Gathering of Materials:
The research project on the efficacy of an alternative hand sanitizer formula gathered essential
ingredients, including Lemon and Aloe Vera. The lemon were used in this research can be acquired
in the various fruits store in the sampol market, while Aloe Vera gel was sourced from members
cultivating Aloe Vera plants. These ingredients underwent meticulous extraction processes to obtain
their beneficial components. In addition to these natural elements, the researchers incorporated Tea
Tree Oil for its potent antimicrobial properties. Glycerin, essential for skin hydration, was thoughtfully
included, and Xanthan Gum was used as a thickener to achieve the desired gel-like consistency.
Distilled water was skillfully employed to dilute the mixture appropriately. Notably, isopropyl alcohol,
an integral component of conventional sanitizers, was thoughtfully omitted in this alcohol-free
formulation. All necessary containers, equipment, and tools were thoughtfully provided by the
research team, while glycerin and containers were procured from a local pharmacy and the online
store Shopee, while the equipment and tools were also provided by the researchers.

Extraction of essential of from Lemon and Aloe Vera:


The extraction process of essential components from Lemon and Aloe Vera is a crucial step in our
research on alternative hand sanitizer formulation. To extract lemon essential oil, we will collect fresh
lemon peels and subject them to a steam distillation process. This process will yield fragrant lemon
essential oil, which contains citric acid and possesses antibacterial properties. For Aloe Vera, leaves
were cut and remove the outer green skin layer with the use of knife, filleting the skin away from the
gel inside. After removing skin, they cut the aloe vera gel stripe into cubes, then placed into blender
and blended into a gel. After that, the gel produced should set aside for the next process we will
extract the gel from mature Aloe Vera leaves by carefully slicing them open and scooping out the gel.
Both extraction processes will be conducted meticulously to ensure the purity and effectiveness of the
components obtained, contributing to the success of our research.

Stage 3
Preparation of Set-ups:
The preparation set-ups for our experiment involve carefully arranging and organizing all the
necessary materials, ingredients, and equipment before commencing our research. This crucial
phase ensures that we have everything ready and in place for a smooth and efficient experimentation
process.
Making Set-ups:
The making set-ups phase entails the hands-on creation of our experimental setups based on the
established procedures and protocols. During this stage, we meticulously assemble, combine, and
process the ingredients and components to construct our experimental setups, following precise
guidelines to achieve accurate and reliable results.

Running Experiment:
At present, our experiment is in progress, with the carefully prepared setups and meticulously
collected data actively undergoing analysis and observation. Our research team is diligently
monitoring various parameters, conducting measurements, and recording observations to ensure the
experiment's accuracy and reliability. We are excited to observe the outcomes and draw meaningful
conclusions from the ongoing experiment, which promises to contribute valuable insights to our study.

Stage 4
Testing of Produced hand sanitizer:
After the production of anti-bacterial hand sanitizer in different set-ups, our research will test its
efficiency and effectiveness through observation. Our researchers will test the produced hand
sanitizer using the Moldy Bread Experiment, where the bread is being touched with the produced
product to see if the bread will have mold after a few days. We prepared fresh bread for each set-up.
For the experiment, we placed three different slices of bread for each set-up in separate baggies.
One bread slice was put in the baggie untouched or being a control, one was touched with dirty
hands, and the other one was touched by the researcher who used hand sanitizer. We observed the
bread forming mold on each set-up after a few days. After this process, we will now come up with a
conclusion.

Data Gathering:
During the experimentation process, the researchers recorded data and their observation. The
recording methods are in the form of a video and picture. On the other hand, after the
experimentation, they gathered all need and necessary data that has benefit the project.

Result analysis and interpretation:


The result analysis and interpretation phase involves scrutinizing the data collected, including
observations from the Moldy Bread Experiment and any other relevant findings. It aims to discern
patterns, correlations, and trends in the data, helping to evaluate the effectiveness of the natural hand
sanitizer. The analysis will determine if there are significant differences in mold growth between the
control, dirty hands, and hand sanitizer-treated bread slices, providing insights into the sanitizer's
antimicrobial properties.

Conclusion:
The experiment demonstrated that the hand sanitizer formulated with Citrus Limon (lemon) and Aloe
Vera exhibited notable effectiveness in inhibiting mold growth on bread slices, comparable to
conventional hand sanitizers. This suggests that the natural ingredients in the sanitizer may possess
antimicrobial properties suitable for hand hygiene. Further research and testing may be warranted to
validate these findings and explore the potential benefits of this chemical-free alternative hand
sanitizer.

As our research journey unfolds, we invite you to join us in discovering the exciting
possibilities of nature's contribution to a healthier tomorrow.

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