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A202001006 - PUTRI WAHYUNI - Primer3 Output (Primer3 - Results - Cgi Release 4.1.0)

The document summarizes the results of a primer picking analysis run through Primer3. It identifies two optimal primer pairs for a 1,437 bp template sequence. The first primer pair has primers starting at positions 82 and 308 that are 20 bases long with melting temperatures near 59°C and produce a 227 bp product. Three additional optional primer pairs are also listed that could be used.

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74 views2 pages

A202001006 - PUTRI WAHYUNI - Primer3 Output (Primer3 - Results - Cgi Release 4.1.0)

The document summarizes the results of a primer picking analysis run through Primer3. It identifies two optimal primer pairs for a 1,437 bp template sequence. The first primer pair has primers starting at positions 82 and 308 that are 20 bases long with melting temperatures near 59°C and produce a 227 bp product. Three additional optional primer pairs are also listed that could be used.

Uploaded by

sasya
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
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7/17/23, 8:10 AM Primer3 Output (primer3_results.cgi release 4.1.

0)

Primer3 Output

PRIMER PICKING RESULTS FOR

Template masking not selected


No mispriming library specified
Using 1-based sequence positions
OLIGO start len tm gc% any_th 3'_th hairpin seq
LEFT PRIMER 82 20 59.01 50.00 0.00 0.00 0.00 AACATACCCTTTCCTGCGGA
RIGHT PRIMER 308 20 58.96 55.00 0.00 0.00 0.00 CAATATTCCCCACTGCTGCC
SEQUENCE SIZE: 1437
INCLUDED REGION SIZE: 1437

PRODUCT SIZE: 227, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

1 AACGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAACGCCCCGCAAGGGGAGTGGCA

61 GACGGGTGAGTAACGCGTGGGAACATACCCTTTCCTGCGGAATAGCTCCGGGAAACTGGA
>>>>>>>>>>>>>>>>>>>>

121 ATTAATACCGCATACGCCCTACGGGGGAAAGATTTATCGGGGAAGGATTGGCCCGCGTTG

181 GATTAGCTAGTTGGTGGGGTAAAGGCCTACCAAGGCGACGATCCATAGCTGGTCTGAGAG

241 GATGATCAGCCACATTGGGACTGAGACACGGCCCAAACTCCTACGGGAGGCAGCAGTGGG
<<<<<<<<<<<<

301 GAATATTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGAGTGATGAAGGCCTT
<<<<<<<<

361 AGGGTTGTAAAGCTCTTTCACCGATGAAGATAATGACGGTAGTCGGAGAAGAAGCCCCGG

421 CTAACTTCGTGCCAGCAGCCGCGGTAATACGAAGGGGGCTAGCGTTGTTCGGAATTACTG

481 GGCGTAAAGCGCACGTAGGCGGATATTTAAGTCAGGGGTGAAATCCCGCAGCTCAACTGC

541 GGAACTGCCTTTGATACTGGGTATCTTGAGTATGGAAGAGGTAAGTGGAATTCCGAGTGT

601 AGAGGTGAAATTCGTAGATATTCGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGTCCA

661 TTACTGACGCTGAGGTGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCC

721 ACGCCGTAAACGATGAATGTTAGCCGTCGGGCAGTATACTGTTCGGTGGCGCAGCTAACG

781 CATTAAACATTCCGCCTGGGGAGTACGGTCGCAAGATTAAAACTCAAAGGAATTGACGGG

841 GGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGCAGAACCTTACCAG

901 CTCTTGACATTCGGGGTATGGGCATTGGAGACGATGTCCTTCAGTTAGGCTGGCCCCAGA

961 ACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAAC

1021 GAGCGCAACCCTCGCCCTTAGTTGCCAGCATTTAGTTGGGCACTCTAAGGGGACTGCCGG

1081 TGATAAGCCGAGAGGAAGGTGGGGATGACGTCAAGTCCTCATGGCCCTTACGGGCTGGGC

https://primer3.ut.ee/cgi-bin/primer3/primer3web_results.cgi 1/2
7/17/23, 8:10 AM Primer3 Output (primer3_results.cgi release 4.1.0)

1141 TACACACGTGCTACAATGGTGGTGACAGTGGGCAGCGAGACAGCGATGTCGAGCTAATCT

1201 CCAAAAGCCATCTCAGTTCGGATTGCACTCTGCAACTCGAGTGCATGAAGTTGGAATCGC

1261 TAGTAATCGCAGATCAGCATGCTGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCC

1321 GTCACACCATGGGAGTTGGTTTTACCCGAAGGTAGTGCGCTAACCGCAAGGAGGCAGCTA

1381 ACCACGGTAGGGTCAGCGACTGGGGTGAAGTCGTAACAAGGTAGCCGTAGGGGAACC

KEYS (in order of precedence):


>>>>>> left primer
<<<<<< right primer

ADDITIONAL OLIGOS
start len tm gc% any_th 3'_th hairpin seq

1 LEFT PRIMER 908 20 59.04 55.00 0.00 0.00 0.00 CATTCGGGGTATGGGCATTG


RIGHT PRIMER 1102 20 58.88 55.00 0.00 0.00 0.00 CCACCTTCCTCTCGGCTTAT
PRODUCT SIZE: 195, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

2 LEFT PRIMER 1034 20 59.10 50.00 0.00 0.00 0.00 GCCCTTAGTTGCCAGCATTT


RIGHT PRIMER 1251 20 58.85 50.00 24.18 24.18 0.00 ACTTCATGCACTCGAGTTGC
PRODUCT SIZE: 218, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

3 LEFT PRIMER 732 20 58.78 55.00 14.38 0.00 0.00 GATGAATGTTAGCCGTCGGG


RIGHT PRIMER 927 20 59.04 55.00 0.00 0.00 0.00 CAATGCCCATACCCCGAATG
PRODUCT SIZE: 196, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

4 LEFT PRIMER 1232 20 58.85 50.00 20.85 0.00 0.00 GCAACTCGAGTGCATGAAGT


RIGHT PRIMER 1428 20 58.75 50.00 0.00 0.00 0.00 ACGGCTACCTTGTTACGACT
PRODUCT SIZE: 197, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

Statistics
con too in in not no tm tm high high high high
sid many tar excl ok bad GC too too any_th 3'_th hair- poly end
ered Ns get reg reg GC% clamp low high compl compl pin X stab ok
Left 7824 0 0 0 0 279 0 2044 2752 0 1 25 51 0 2672
Right 7823 0 0 0 0 209 0 2054 2734 0 0 14 52 0 2760
Pair Stats:
considered 1002, unacceptable product size 995, primer in pair overlaps a primer in a better pair 126, ok 7
libprimer3 release 2.4.0

(primer3_results.cgi release 4.1.0)

https://primer3.ut.ee/cgi-bin/primer3/primer3web_results.cgi 2/2

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