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Data Analysis (Draft Practice-Complete Kimmy)

The document describes experiments measuring the antibacterial activity of extracts from different parts of Zephyranthes sp. against Klebsiella pneumoniae. It finds that the stem extract showed the highest mean zone of inhibition at 15.667 mm, followed by leaf, root, and flower extracts. A one-way ANOVA analysis found a significant difference in antibacterial activity between the plant extracts with a p-value less than 0.05. The document also describes an experiment correlating growth of a mutant plant to temperature, finding a positive correlation, and generating a calibration curve to determine zinc concentrations in unknown samples using UV-Vis spectrophotometry.

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Kurt Leonard Albao
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13 views

Data Analysis (Draft Practice-Complete Kimmy)

The document describes experiments measuring the antibacterial activity of extracts from different parts of Zephyranthes sp. against Klebsiella pneumoniae. It finds that the stem extract showed the highest mean zone of inhibition at 15.667 mm, followed by leaf, root, and flower extracts. A one-way ANOVA analysis found a significant difference in antibacterial activity between the plant extracts with a p-value less than 0.05. The document also describes an experiment correlating growth of a mutant plant to temperature, finding a positive correlation, and generating a calibration curve to determine zinc concentrations in unknown samples using UV-Vis spectrophotometry.

Uploaded by

Kurt Leonard Albao
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as XLSX, PDF, TXT or read online on Scribd
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different types of Zephyranthes sp.

MeOH extracts
against Klebsiella pneumoniae (disc = 6 mm)
Trials
Extracts 1 2 3 Mean SD
Leaf 13 12 14 13 1
Flower 8 9 6 7.666667 1.527525
Root 11 12 13 12 1
Stem 14 16 17 15.66667 1.527525

Positive
control
(Zinc
Oxide) 17 18 20 18.33333 1.527525

Negative
control
(distilled
water) 0 0 0 0 0

Ho:
Ha:

ANOVA
alpha 0.05
p-value
Decision
Inference

Anova: Single Factor

SUMMARY
Groups Count Sum Average Variance
Leaf 3 39 13 1
Flower 3 23 7.666667 2.333333
Root 3 36 12 1
Stem 3 47 15.66667 2.333333
Positive co 3 55 18.33333 2.333333
Negative co 3 0 0 0

ANOVA
Source of VSS df MS F P-value F crit
Between G 637.7778 5 127.5556 85.03704 6.051E-09 3.105875
Within Gro 18 12 1.5

Total 655.7778 17
Table 2. Response of
Mutant Plant X in terms of
growth at temperature
range 34-39 C for 28 days
SampleTemperature
No.Growth ofinMutant
C Plant X (m) Test of Correlation:
1 34 12 Correlation coeffecient:
2 34 13 Positive or Negative correlation:
3 34 12 Inference:
4 34 13
5 34 14
6 35 15 Sample No.
Temperature
Growth ofinMutant
C Plant X (m)
7 35 16 Sample No. 1
8 35 13 Temperatur0.986562 1
9 35 12 Growth of 0.737809 0.722363 1
10 35 15
11 36 17
12 36 17
13 36 16
14 36 15
15 36 14
16 37 15
17 37 14
18 37 16
19 37 14
20 37 15
21 38 16
22 38 17
23 38 18
24 38 18
25 38 20
26 39 19
27 39 13
28 39 18
29 39 22
30 39 23
Table 3. Generating calibration curve
with the following concentrations of
ZnO in Molarity (M) using UV-VIS
spectrophotometer (450nm)
Concentration
Absorbance
(M)
Absorbance
Generated linear equation
1 0.789 slope: 0.9

0.8 0.6 y-intercept: 0.8


0.6 0.54 equation: 0.7 f(x) = 0.535662359618404 x + 0.226109382924768
0.4 0.49 0.6
0.2 0.34 0.5
0.54
0.49
0.01 0.21 SLOPE 0.535662
0.4
INTERCEP 0.226109 0.34
0.3
Unknown Sample
0.2 0.21
No. AbsorbanceConcentration (M)
1 0.31 0.1
2 0.508 0
0 0.2 0.4 0.6 0.
3 0.29
4 0.672 R 0.983373

SUMMARY OUTPUT

Regression Statistics
Multiple R 0.983373
R Square 0.967023
Adjusted R 0.958779
Standard E 0.041086
Observatio 6

ANOVA
df SS MS F Significance F
Regression 1 0.198008 0.198008 117.2975 0.000412
Residual 4 0.006752 0.001688
Total 5 0.204761

Coefficients
Standard Error t Stat P-value Lower 95%Upper 95%Lower 95.0%
Upper 95.0%
Intercept 0.226109 0.02995 7.549641 0.001649 0.142956 0.309263 0.142956 0.309263
X Variable 0.535662 0.049459 10.8304 0.000412 0.398342 0.672983 0.398342 0.672983
Absorbance

0.789
18404 x + 0.226109382924768

0.6
0.54
0.49

0.4 0.6 0.8 1 1.2


Table 3. Generating calibration curve
with the following concentrations of
ZnO in Molarity (M) using UV-VIS
spectrophotometer (450nm)
0.9 Absorban
0.8
Concentration
Absorbance
(M) Generated linear equation SUMMARY OUTPUT
0.7 f(x) = 0.535662359618404 x + 0.226109382
1 0.789 slope: R² = 0.967023237697032
0.6
0.8 0.6 y-intercept: Regression Statistics
0.6 0.54 equation: 0.5 Multiple R
0.4 0.49 0.4 R Square
0.2 0.34 0.3 Adjusted R
0.01 0.21 SLOPE 0.535662 0.2 Standard E
INTERCEP 0.226109 0.1 Observatio
Unknown Sample 0
No. AbsorbanceConcentration (M) 0 0.2
ANOVA 0.4 0.6
1 0.31 0.392167
2 0.508 0.498236 Regression
3 0.29 0.381453 Residual
4 0.672 0.58609 Total

Coefficients
Intercept
X Variable
Absorbance
SUMMARY OUTPUT
.535662359618404 x + 0.226109382924768
967023237697032
Regression Statistics
0.983373
0.967023
0.958779
0.041086
6

0.2 0.4 0.6 0.8 1 1.2

df SS MS F Significance F
1 0.198008 0.198008 117.2975 0.000412
4 0.006752 0.001688
5 0.204761

Coefficients
Standard Error t Stat P-value Lower 95%Upper 95%Lower 95.0%
Upper 95.0%
0.226109 0.02995 7.549641 0.001649 0.142956 0.309263 0.142956 0.309263
0.535662 0.049459 10.8304 0.000412 0.398342 0.672983 0.398342 0.672983

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