This document lists and describes various instruments, materials, and equipment that are commonly used in biochemistry laboratories, including test tubes, beakers, graduated cylinders, round bottom flasks, Erlenmeyer flasks, watch glasses, dropper pipettes, burettes, distilling flasks, Graham condensers, volumetric flasks, and Petri dishes. It provides examples of how each item is used, such as using test tubes to handle chemicals or graduated cylinders to measure liquid volumes. The purpose of this document is to outline the core tools and containers needed to conduct experiments and assays involving biological and chemical samples in a biochemistry lab setting.
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Lab Apparatuses
This document lists and describes various instruments, materials, and equipment that are commonly used in biochemistry laboratories, including test tubes, beakers, graduated cylinders, round bottom flasks, Erlenmeyer flasks, watch glasses, dropper pipettes, burettes, distilling flasks, Graham condensers, volumetric flasks, and Petri dishes. It provides examples of how each item is used, such as using test tubes to handle chemicals or graduated cylinders to measure liquid volumes. The purpose of this document is to outline the core tools and containers needed to conduct experiments and assays involving biological and chemical samples in a biochemistry lab setting.
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BIOCHEMISTRY
LABORATORY INSTRUMENTS/MATERIALS/ EQUIPMENT NEEDED IN BIOCHEMISTRY LABORATORY
INSTRUMENTS/MATERIALS/ USE/FUNCTION EXAMPLE OF
EQUIPMENT CHEMICAL MIXTURE Test Tube Test tubes are The purpose of often used to test tubes are to conduct live handle chemicals, experiments as especially for well, including the qualitative growth and testing experiments and of microbes assays. (microscopic organisms) such as bacteria and small fungi. Also, test tubes may be used to collect biological samples, including blood, saliva, and urine. Beaker Beakers are useful Beakers are as a reaction useful as a container or to hold reaction container liquid or solid or to hold liquid or samples. solid samples. They are also used to catch liquids from titrations and filtrates from filtering operations. Graduated Cylinder Graduated Graduated cylinders are long, cylinders are long, slender vessels slender vessels used for measuring used the volumes of for measuring the liquids. volumes of liquids. They are not intended for mixing, stirring, heating, or weighing.
Round Bottom Flasks Round-bottom It is most often
flasks are often used when heating used to contain solutions, chemical reactions particularly for run by chemists, distillation. especially for reflux set-ups and laboratory-scale synthesis. Erlenmeyer Flask Erlenmeyer flasks The flask is often are used to contain used for stirring or liquids and for heating mixing, heating, solutions and is cooling, incubation, purposefully filtration, storage, designed to be and other liquid- useful for those handling tasks. processes. Watch Glasses Watch glasses are A watch glass is a circular, slightly- round, concave concave pieces of glass dish used glass that are used for evaporation in by chemists to chemistry. It can evaporate liquids also be employed and cover beakers for weighing solids during sample and as a lid for preparation. They flasks and are also used to beakers. hold solids during weighing.
Dropper Pipettes Dropper pipettes The purpose is to
are generally used transfer small for moving small quantities of amounts of liquid liquids. They are or when measuring used in the and dispensing laboratory and liquid in mL units. also to dispense small amounts of liquid medicines. A very common use was to dispense eye drops into the eye. Burette A burette is A burette is used to dispense used to dispense small volumes of small volumes of liquid called liquid called aliquots, or aliquots, or sometimes gas, sometimes gas, with high accuracy. with high accuracy. It consists of a long glass tube with a valve at one end to control the flow of liquid. Burettes serve essentially the same purpose as a pipette. Distilling Flask It is primarily used It is primarily used for distillation, the for distillation, the process of process of separating a separating a mixture of liquids mixture of liquids with different with different boiling points boiling points through through evaporation and evaporation and condensation. condensation.
Graham Condenser for A Graham A Graham
Distillation condenser is condenser is used to cool and used to cool and condense a gas condense a gas back to a liquid, back to a liquid, often as part of the often as part of the process of process of chemical chemical distillation. distillation. The piece consists of a coiled glass tube through which the gas travels. The coil is surrounded by a jacket of water that helps to cool the gas. Volumetric Flasks Volumetric flasks A volumetric flask are used for is lab glass or accurate dilutions plasticware used and preparation of to prepare a solutions and any solution. It is other liquids used to make up a needed at the solution to a laboratory known volume. workflow. Volumetric flasks are used to measure volumes much more precisely than beakers or Erlenmeyer flasks.
Petri Dish Petri dish is a A Petri dish is a
shallow cylindrical, shallow round glass that is transparent glass used in or plastic laboratories to cylindrical lidded culture different dish, used to microorganisms contain a thin layer and cells. of agar on which to grow bacteria and fungi. Millions of Petri dishes are used in microbiology labs every year. Evaporating Dish Evaporating Evaporating Dishes are Dishes are glassware glassware containers used for containers used laboratory testing. for laboratory They are used for testing. They are the evaporation of used for the liquids and evaporation of solutions to liquids and produce a solid solutions to substance or produce a solid concentrated substance or solutions. concentrated solutions. Dishes have a shallow flat round surface to promote evaporation. Spot Plate Use spot plates Use spot plates (also called (also called reaction plates or reaction plates or culture plates) to culture plates) to perform reactions perform reactions on a small amount on a small amount of chemicals. of chemicals. Each features many depressions (or wells), either 12 or 24, in which to perform the reaction. Crucible and Cover Enable chemical Crucible is used in compounds to be the laboratory to heated to very high contain chemical temperatures. compounds when heated to extremely high temperatures. Crucibles are available in several sizes and typically come with a correspondingly- sized lid. A graduated Graduated pipette is a pipette Pipettes are types Graduated Pipette with its volume, in of macro pipettes. increments, They marked along the can accurately tube. It is used to measure volumes accurately of liquids larger measure and than micropipettes transfer a volume can handle. Just of liquid from one like any other container to pipettes, another. graduated pipettes are made either with plastic or borosilicate glass. It has a tapered tip and a cylinder tube bearing the volume markings for measurement. Pipestem Triangle The Pipestem Wire gauzes are triangle is used to used to support support a crucible larger objects, while it is being such as beakers heated over a and evaporating Bunsen burner. It dishes while they is very heat are being heated. resistant and can be used at high temperatures. Bunsen Burner Used for heating, A heat source in sterilization, and laboratory combustion. experiments Laboratory Tripod A laboratory tripod To support flasks is a three-legged and beakers. platform used to support flasks and beakers.
Electronic Balance Electronic balance Instrument for the
is an instrument laboratories for used in precise the accurate measurement of measurement of chemicals which weight of are used in various materials. experiments
Retort Support Stand Used to support To support other
other pieces of pieces of equipment and equipment and glassware for glassware instance, burettes, test tubes and flasks.
2 and 3 Fingered Clamps The small size is Used to hold larger
typically used to pieces of hold small thin glassware and cylindrical items odd-shaped such as objects thermometers and temperature probes. Clamps Holder A clamp holder in a To secure lab is used to laboratory clamps secure an extension-type utility clamp to a support stand or ring stand.
Tube Holder A test tube holder To hold test tubes.
is used to hold test It is used for tubes. It is used for holding a test tube holding a test tube in place when the in place when the tube is hot or tube is hot or should not be should not be touched touched.
Test Tube Rack A test tube rack is used to hold
a piece of multiple test tubes laboratory upright at the equipment used to same time hold multiple test tubes upright at the same time.
Aspirator A device for Used for removing
removing liquids or obstructions gases by suction.
Laboratory Thermometer A laboratory Thermometer
thermometer Used for is used to measure measuring the boiling point temperatures other and freezing point than the human during science body temperature experiments.
Glass Stirring Rod A glass stirring rod Used to help
is used to stir or decant the liquid mix solutions. from a container while retaining a solid in the original flask
Laboratory Rubber Stoppers Rubber stopper To prevent a gas
is to prevent a gas or liquid from or liquid from escaping its escaping its container during a container during a scientific scientific experiment experiment. Pipe Cleaning Brush A test tube brush To clean out pipes or spout brush is a and other long brush used hollow pipelines for cleaning test tubes and narrow mouth laboratory glassware, such as graduated cylinders, burettes, and Erlenmeyer flasks.