Clinical Microscopy

Leica Microsystems` traditional and digital microscopy solutions for clinical and life science research
applications focus on the user. You can expect detail-enhancing clarity from exceptional optics and
all-day comfort from advanced ergonomics.

Leica’s clinical microscopes help you see all the finest details required for a precise and accurate
diagnosis, while helping you achieve high-quality results in your scientific research.

Why use Leica microscopes for Clinical Microscopy?

Experience optimal ergonomic operation of a microscope thanks to

symmetrical layout of coaxial drive and focus knobs

Change slides and adjust focus and position of the stage with one hand

Stage is rounded with no protruding edges. The entire stage design is

compact and requires minimal space

The stage plate is built to last. The surface is made of a durable, hard
ceramic that is built to take years of demanding use

About Leica Clinical Microscopy Solutions

Leica DM Microscopes are designed for accurate clinical diagnostics. While they are ideal for routine
tasks, there is no compromise in the performance or range of features for more advanced
techniques. With a rugged, solid design combined with excellent optics, Leica DM Microscopes can
be equipped with a full range of accessories. Leica can truly personalize a microscope to an
individual user’s unique needs.

BIOTECHNIQUES • CHROMTECHNIQUES • WATERTECHNIQUES • BIOPHARMTECHNIQUES • ROBOTIQUES

Johannesburg (011) 919 1000 | Cape Town (021) 557 9480

PO Box 4181, Randburg, 2125 | Head Office: 9 Will Scarlet Rd, Robindale, 2194 | [email protected]

Separations (Pty) Ltd Directors: Paul Queisser

Registration # 1988/007162/07 Hans Queisser
VAT # 4810245292 Nicolas Queisser
 Clinical Microscopy Products specially designed for Invitro Laboratories:

STEREO MICROSCOPE RANGE

Looking at the Sperm – Counting and Sperm Viability

Leica DM 500 and DM 750

Binocular, fluorescence-capable microscope with Phase contrast and

DIC methods

Available with the ICC50 Wifi camera with LAS software integrated in
the camera module

Features such as a pre-focused, pre-centered condenser and the

EZTube™ preset diopter available on the DM500 and the EZLite™ on the DM750, which provides
over 20 years of LED illumination and automatic time delay shutoff, saves time and energy.

Leica Microsystems has integrated an additive to the material of all

microscope touchpoints to inhibit the growth of bacteria. The AGTREAT
therefore prevents the spread of disease and leads to a healthier
laboratory environment.

Use these models in your first steps to view, count and check healthy
sperm - YOU can chose to view directly through the microscope or
attach the camera and view on a screen

BIOTECHNIQUES • CHROMTECHNIQUES • WATERTECHNIQUES • BIOPHARMTECHNIQUES • ROBOTIQUES

Johannesburg (011) 919 1000 | Cape Town (021) 557 9480

PO Box 4181, Randburg, 2125 | Head Office: 9 Will Scarlet Rd, Robindale, 2194 | [email protected]

Separations (Pty) Ltd Directors: Paul Queisser

Registration # 1988/007162/07 Hans Queisser
VAT # 4810245292 Nicolas Queisser
 Greenough Stereo Microscopes S9 Series

S APO unique Greenough Stereo Microscope with high magnification power

The Greenough stereo microscope, S APO with apochromatic 8:1 zoom and high magnification up to
80x is ideal for quality control, cell sorting, and microinjection applications.

With 75 mm working distance it provides easy access to the sample.

An ergonomic viewing angle of 38 degrees allows comfortable
posture, which helps to increase productivity and reduce fatigue
related inspection errors. Adjustable zoom stops allow fast, easy,
repeatable measurements and inspections.

The integrated documentation port allows connection of a digital

camera.

Changing perspective is easy

Do you sometimes find it difficult to work only through the

eyepieces of the microscope?

With the digital functionality of the S9 i, S9 D, and S APO

you don’t have to choose between screen or eyepieces. You
can quickly scan through samples using a large screen view
and check on details through the eyepieces of the
microscope.

BIOTECHNIQUES • CHROMTECHNIQUES • WATERTECHNIQUES • BIOPHARMTECHNIQUES • ROBOTIQUES

Johannesburg (011) 919 1000 | Cape Town (021) 557 9480

PO Box 4181, Randburg, 2125 | Head Office: 9 Will Scarlet Rd, Robindale, 2194 | [email protected]

Separations (Pty) Ltd Directors: Paul Queisser

Registration # 1988/007162/07 Hans Queisser
VAT # 4810245292 Nicolas Queisser
 INVERTED MICROSCOPE RANGE

MICROMANIPULATION

Micromanipulation

In many time-lapse or multi-dimensional experiments, cell manipulation is the starting point for
subsequent analysis. Microinjection with DNA, RNA or probes into adherent cells enable you to
better understand pathways and intracellular machinery. DNA, stem cells or spermatozoa
microinjection into oocytes or blastocysts are performed to create transgenic or cloned organisms,
or to fertilize oocytes for Assisted Reproductive Technology (ART), such as in vitro fertilization (IVF).

Furthermore the CRISPR/Cas9 technique is used to produce transgene animals. With several Leica
configurations to meet your needs and budget, you will be sure to find the perfect
micromanipulation solution.

Brilliant Image Quality

Visualize small structures, such as sperm heads, with the

highest resolution and contrast. With superb contrast
methods, such as Integrated Modulation Contrast and
DIC, and a variety of high quality objectives to choose
from, you will see tiny structures with clarity.

Keep the Specimen in View

Zoom in and out without switching objectives and losing

sight of moving specimens. The Leica variozoom camera
c-mount allows you to increase and decrease the
magnification with just a turn of the adaptor - perfect to
keep fast moving specimen like spermatocytes in view
while changing magnification to check morphology or
catch a sperm for injection.

BIOTECHNIQUES • CHROMTECHNIQUES • WATERTECHNIQUES • BIOPHARMTECHNIQUES • ROBOTIQUES

Johannesburg (011) 919 1000 | Cape Town (021) 557 9480

PO Box 4181, Randburg, 2125 | Head Office: 9 Will Scarlet Rd, Robindale, 2194 | [email protected]

Separations (Pty) Ltd Directors: Paul Queisser

Registration # 1988/007162/07 Hans Queisser
VAT # 4810245292 Nicolas Queisser
Inverted Laboratory Microscope Leica DM IL LED

With high-performance optics, ergonomic design, and 5W LED illumination,

the Leica DM IL LED is ideal for cell culture, micromanipulation,
documentation of immunostained specimens, and routine live cell
examinations.

The Leica DM IL LED features a comprehensive set of contrast methods to

monitor your specimen the way you need. High-quality Phase contrast,
excellent modulation contrast and brilliant fluorescence are just one
fingertip away. Robust stability, plenty of space to work with tools, long
working distances to accommodate large culture flasks and a stable
illumination without heat make work at the microscope easy and
convenient.

For special diagnostics requirements, the microscope is certified for in-vitro-diagnostics (IVD)
including in-vitro-fertilization (IVF).

Contrasting methods

In addition to Phase Contrast and Brightfield illumination, the DM

IL features unique Integrated Modulation Contrast (IMC) from
Leica without the need for special objectives.

Available for 1 0x, 20x, 32x, and 40x magnification.

BIOTECHNIQUES • CHROMTECHNIQUES • WATERTECHNIQUES • BIOPHARMTECHNIQUES • ROBOTIQUES

Johannesburg (011) 919 1000 | Cape Town (021) 557 9480

PO Box 4181, Randburg, 2125 | Head Office: 9 Will Scarlet Rd, Robindale, 2194 | [email protected]

Separations (Pty) Ltd Directors: Paul Queisser

Registration # 1988/007162/07 Hans Queisser
VAT # 4810245292 Nicolas Queisser
 Inverted Laboratory Microscope Leica DMi8

Perfect Stability

Creating a vibration-free setup and obtaining outstanding

optics to visualize tiny particles for microinjection (e.g.
pronuclei) are the major challenges for micromanipulation.

Offering superior precision, Mechanical Manipulators by Leica

Microsystems are ideal for life science applications where
micro surgical, physiological, or chemical operations are
performed on live organisms such as oocytes, adherent cells,
and plant cells. Typical applications include microinjection in adherent cells, transgenics, and work
involving stem cells.

Leica DMi8 offers a stable platform for micromanipulation

The Leica DMi8 offers a stable, ergonomic, and flexible microscope

platform along with a variety of contrasting methods for visualizing
cells. In combination with the micromanipulator of choice, you can
create a perfectly matched system to work with your cells.

Various Configurations examples:

Configuration example for ICSI

Leica Microsystems and Narishige: a widely used combination

around the world. With both manual and motorized oil hydraulic Narishige micromanipulators
available, find the option that works best for your needs.

Configuration example for pronucleus injection

DMi8 equipped with our mechanical micromanipulators is the workhorse for precision and
robustness. The joystick movement transfers directly to the tip of the capillary, without any
backlash.

Configuration example for embryonic stem cell transfer

Fully Motorized Micromanipulation: The fully motorized Leica DMi8 and Eppendorf
micromanipulators allow you to store and recall important functions, thereby increasing speed and
precision. A touch screen can be added for easy and intuitive control of all microscope functions.

BIOTECHNIQUES • CHROMTECHNIQUES • WATERTECHNIQUES • BIOPHARMTECHNIQUES • ROBOTIQUES

Johannesburg (011) 919 1000 | Cape Town (021) 557 9480

PO Box 4181, Randburg, 2125 | Head Office: 9 Will Scarlet Rd, Robindale, 2194 | [email protected]

Separations (Pty) Ltd Directors: Paul Queisser

Registration # 1988/007162/07 Hans Queisser
VAT # 4810245292 Nicolas Queisser
Our Success Story

Separations have supported Leica Microsystems for the past

2 years and have grown from strength to strength. We have
supplied various models to all fields in many different
industries that require microscopy.

By far one of our greatest successes has been the installation of the DMi8 at the University of
Pretoria in Ondersterpoort, for Mr Mario Smits who is focusing on IVF in animals.

We Installed the Manual DMi8 from Leica which was coupled to the Narishige Micromanipulators.
Our Leica Product Specialist is 1 of the 2 Narishige certified operators in South Africa with intimate
knowledge on how these accessories work and just how well they marry to the Leica Microscopes.

Below is some pictures of unit installed and images that were taken using this system.

BIOTECHNIQUES • CHROMTECHNIQUES • WATERTECHNIQUES • BIOPHARMTECHNIQUES • ROBOTIQUES

Johannesburg (011) 919 1000 | Cape Town (021) 557 9480

PO Box 4181, Randburg, 2125 | Head Office: 9 Will Scarlet Rd, Robindale, 2194 | [email protected]

Separations (Pty) Ltd Directors: Paul Queisser

Registration # 1988/007162/07 Hans Queisser
VAT # 4810245292 Nicolas Queisser