BIO250L V2 Lab 1
BIO250L V2 Lab 1
“Pre-Lab Questions”
1. What constitutes personal protective equipment? When should personal protective equipment
be worn? Why is personal protective equipment important?
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2. Why are there increasing levels of biological containment procedures? What level of biological
containment will be necessary to complete the experiments in this manual?
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3. What document should you refer to if you have questions regarding the safety or disposal of a
chemical? Where can this document be located?
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4. List five safety procedures that should always be adhered to when working with
microorganisms.
2. Develop a hypothesis relating to the pH level of the culture media and the number of microbial
colonies observed in each culture.
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6. What type of graph would be appropriate for this data set? Why?
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“Lab 1 Introduction to Science BIO250L”
7. Graph the data from Table 4. Include your name and access code handwritten on your graph.
a. Observation is .
2. Sally comes to work sick; two days later, three of her coworkers are also sick.
a. Observation is .
3. You accidentally left a carton of milk on the counter all night, and you notice that the milk tastes
worse than it usually does when it is stored in the refrigerator.
a. Observation is .
2. You want to make sure your incubator is operating at the correct temperature of 37˚C, so you
place a thermometer inside the incubator and check it every hour for five hours. You record
readings of 36.9˚C, 36.9˚C, 37.1˚C, 37.0˚C, and 37.1˚C.
3. You aren’t sure whether or not your pH meter needs to be calibrated, so you put it in a solution
that you know has a pH of 7. Take four separate readings, which are reported as 5.5, 8.6, 7.2,
and 9.4.
4. Your lab is working on sequencing a new plasmid. Before starting, you all decide to guess how
many base pairs you think the new plasmid has. The lab members’ guesses are 4,005; 4,006;
4,007; and 4,010. It turns out the plasmid has 7,968 base pairs.
5. You try to measure out exactly 5.0 mL of water by eye into five different test tubes. When you
go back and check, you find the amount of water in each tube is 4.8 mL, 5.3 mL, 5.2 mL, 4.8 mL,
and 4.7 mL.
“Lab 1 Introduction to Science BIO250L”
Hand with
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Washing
Deionized
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Water
Post-Lab Questions
1. What purpose did plating the yeast serve in this experiment? What purpose did the water
serve?
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4. Were there any colonies present on plate #2? What could account for the presence of these
colonies?
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“Lab 1 Introduction to Science BIO250L”
Insert a photo of your plates after incubation. Include your name and access code handwritten in the