Scribd
Upload
29 views

Lab Amylase

Syed Farhad Hassan, a third year student with seat number EH2106070, conducted a lab experiment on amylase activity. The objective was to observe how the enzyme amylase catalyzes the hydrolysis of starch into sugars. Amylase activity is influenced by pH and temperature. Test tubes with varying pH levels and temperatures were incubated with starch and amylase solutions, and iodine tests were periodically used to detect remaining starch.

Uploaded by

SYED FARHAD
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
29 views

Lab Amylase

Syed Farhad Hassan, a third year student with seat number EH2106070, conducted a lab experiment on amylase activity. The objective was to observe how the enzyme amylase catalyzes the hydrolysis of starch into sugars. Amylase activity is influenced by pH and temperature. Test tubes with varying pH levels and temperatures were incubated with starch and amylase solutions, and iodine tests were periodically used to detect remaining starch.

Uploaded by

SYED FARHAD
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
You are on page 1/ 3

NAME: SYED FARHAD HASSAN

CLASS: THIRD YEAR


SECTION: B
SEAT NO: EH2106070

LAB: AMYLASE ACTIVITY


Objective:
The objective of this laboratory experiment is to investigate the enzymatic activity of amylase by
observing its ability to catalyze the hydrolysis of starch.

Theory:

Amylase is an enzyme that plays a crucial role in the digestion of carbohydrates. It catalyzes the
hydrolysis of glycosidic bonds in starch, breaking it down into maltose and other oligosaccharides.
The enzyme is produced in various parts of the digestive system, such as the salivary glands and
the pancreas. The activity of amylase is influenced by factors such as pH and temperature.

Method:
Materials:
• Salivary amylase solution
• Starch solution
• Iodine solution (for detecting the presence of starch)
• Test tubes
• Pipettes
• Water bath
• pH buffer solutions (acidic, neutral, and alkaline)
• Thermometer

Procedure:
• Label the test tubes to indicate the conditions being tested (e.g., pH levels or temperatures).
• In each test tube, mix a specific volume of starch solution with a specific volume of salivary
amylase solution.
• Incubate the test tubes in a water bath set to different temperatures or add different pH
buffer solutions to create varied environments.
• At regular intervals, take samples from each test tube and perform the iodine test to check
for the presence of starch.
Observation:

Starch-Iodine Test:
• Add a few drops of iodine solution to a small sample of the reaction mixture.
• Observe the color change. The presence of starch is indicated by a blue-black color, while
the absence of starch shows a lighter color (yellow to brown).

RESULT:

You might also like