Laser Scanning Micros
Laser Scanning Micros
Invited Paper
Ahmct-In conventional light microscopy, images p ~ formed e either it is possible to detect light diffusely scattered from surface
b y d i r e c t ~ o f t h e o ~ j e c t P t a ~ m r g n i f i c l t i o a o r b y i m t g i n g features much smaller in size than the incident scanned beam
theobject0ntoaranote~rad~~theiflumin.aceItth.t
surfacetoandectricdsipd. InlaesscaMingmkmcopy,theobject size. It will be shown how micrometer-sized defects on silicon
orspecimenatrhceisscPnnedpointbypoietbyrfdIrset.bem. wafersurfaces can be detectedwith rasteredlaser Beams
The actual imqe or other p a t i w n t charactexistic of the objectis then focused to 50-100 I.tm in diameter.
@rerated by an dectmnic system. Use of auch scPnned W systems If the application requires a relatively large fieldto be rapidly
exists today in biomedial resmrch, in the semiconductor microelec-
tronicsindwtry,andinvrriedotheridu&ialhpectkmapplications. covered at high resolution, this can bea tedious and error-prone
Inthisprper,wereviewthebasicp~eaoflrserscPnningmiaob task when performed on a conventionalmicroscope by a human
copy, discuss r
dv
ru
rw as compared to m a e conventional light operator. An example would be the inspection of integrated
microsoopy, and illustnte the technique with examples of systems in circuit photomasks. Even when video imaging devices, such as
llse today. vidicons, CCD’s, etc., are coupled to a microscope, limitations
in image content and dynamic range are encountered in many
I. INTRODUCTION:OVERVIEWOF LASER applications. Laserscanning techniques havebeenused to
SCANNING MICROSCOPY overcome these problems. Also, in laser scanning microscopy
grating with a period much less than the scanning spot size in
P1, a scanning spot with a high-frequencyfringe pattern is
incident upon the object. The diffuse radiation collected by
that same lens is imaged back to the grating. Due to contour
variations on theobject, the image will have well-defined grating
2 lines only at those points that are within the depth of focus
of the imaging lens. These areas will pass through the grating
withmaximum intensity. Out-of-focus grating lines, on the
other hand, will be reduced by the grating duty cycle. The
light passing through the grating on the return is collected and
sent to a detector. The resulting signal has a brightfringe
delineating those regions within the depth of focus of the
Fig.4. A two-spot pattern for object differentiation in two dimensions. imaginglens. The overallimage,however,remains infocus
due to therelatively smallflnumber that describes the scanning
system.
The potential advantagesofwavelength control lie, as ex-
pected, in color discrimination. A single scanning spot of
multiple wavelength can be detected by any number of detec-
tors, each with a narrow-bandwidthspectral filter. The obvious
comparison is to a color camera, which can also discriminate
object color. An advantage may be gained with laser scanning
in that multiple detectors can provide scattering angle informa-
tion. Color as a function of scattering angle can be used, there-
fore, to provide additional object information. In some cases,
use of an infrared (IR) or ultraviolet (W)illumination source
maybebeneficial.Lasersources with high brightnessare
availablein these regions.Examplesofpossible applications
Fig.5. The optical arrangement for focus contouring. are transmission studies in silicon (IR) [ 141 or fluorescence
studies [ 151.
mation. Thus the contour information can act as yet another C. Scanning
input to identify the object detail. Three aspects of scanning control are considered. The first
is the scan format, or the path of the scanning illumination
B. Illumination point in the object plane. The second is scan position infor-
A second area of importance in laser microscopy is illumina- mationforaccuratemeasurement, if desired, and the third
tion. Variations in the illuminationspot during the object relates to focus variations of the scanning spot.
scan can be made to maximize the information obtained; these For most applications, a standard raster scan pattern for the
variations can be in spot size, spot shape, and light wavelength, object scan is employed. However, depending on the applica-
providing a high degree of versatility in illumination. Control tion, it may be advantageous to employ a different scanning
of spot shape, as in spot-size control, utilizes information in pattern. This would be particularly true for image processing,
the vicinity of the scanning spot to optimize the information where a standardized scan format is not necessarily required.
obtained. This will be illustrated with a discussion of object As an example, aradial scan foralignment of radiallysymmetric
differentiation and focus contouring. objects would provide information in a more suitable format
Object differentiation using multiple spots provides an exact for subsequent image processing. In other cases, a spiral scan
means for obtaining a signal representing the differential of might be beneficial. Random access capability for the object
object reflectance. This can be done in two dimensions, there- scan is a possibility as well, dependingupon the scanning
by providing a capability that simple electronics differentiation apparatus employed. Basically, control of the scanning pat-
cannot provide. Shown in Fig. 4 is an example of a spot con- tern is a means of varying the format of the data as received
figuration that can be used. A multiplexing scheme must be by the image processor. In doing so, the circuitry for process-
used to separate the signal obtained from each spot. Differen- ing the data can be simplified. Obviously, many of the poten-
tiation in the x direction can be realized by subtraction of 1 tial advantages for scan control wouldbebased uponthe
from 3, while in they direction the result is obtained from the partic& characteristics of the object in question.
difference of 2 and 3 (see Fig. 4). Scan position information implies precise knowledge of the
Focus contouring employs a scanning illumination spot that scanning beam location. Applications for precise measurement
contains a high spatial-frequency pattern. It is atechnique and gauging require accurate information relating the signal in
that delineates a single contour on a diffuse objectwith a time to an object location in space. Using methods of position
bright fringe. Basically, it takes advantage of the short depth monitoring for a scanning laserbeam1161, accurate beam
of focus obtained withlow flnumber optics. A similar method position information to 1 part in 40 000 has beendemonstrated.
is described as a “locus of focus” contour [ 131. Fig. 5 serves This is done by splitting a portion of the beam and interrogat-
to better describe the technique. A scanning beam is brought ing the spot in a separate s c a n n i n g plane with a grating. A
to image plane P1. Planes P1 and P2 are conjugates, so that time-space relationship is then established by the grating, and
the imageof the scanning beam is at the object plane. The drifts of the scanning apparatus or nonlinearities in the scan
imaging lens is chosen to have a low f/number. By placing a w li not affect measurementaccuracy. Fig. 6 illustrates the
644 PROCEEDINGS OF THE IEEE, VOL. 70, NO. 6 , JUNE 1982
'I LASER
U U
Fii. 7. Schematic illustration of the optical system for thin-fiim mark
inspection.
The same results are obtained by using a microscope and ob- ,-ROTATING MULTlFK€lCD UlRROR
BRIGHT
FIELD
ETECTOR MIRROR II
GAL
LENS
ANNULAR
MIRROR
FIELD
MICROSCOPE
OBJECTlVE
....
CCI
that of maximizing the signal due to scattering from particles particle detector. As seen, only an image of the particle itself
while minimizing the signal due to diffraction from the surface appears,making a visual inspection for particles a relatively
relief pattern. To accomplish this, anarray of detectors is simple task.
placed around the periphery of the wafer and positioned such
that only light scattered at angles approaching 90' with respect D. Other Applications
to the surface is detected. Particles scatter a sisnificant amount In this section, other applications of laser scanning micru-
of light at very high angles due to their inherent shape,whereas scopy are reviewed. A system which was developed for the
the higher diffracted orders scattered from the pattern relief inspection of woven fabric is discussed. Semiconductor mate-
are relativelyweak. Further discrimination between the par- rial characterization and biomedical applications are also
ticles and pattern signals is achieved by using a simple com- reviewed.
parator circuit following the detector to eliminate any low-level I ) Woven Fabric Inspection: A system developed forthe
signals from the pattern. inspection of woven fabrics utilizes a scanning scheme similar
A typical image generated by the system is shown in Fig. 13. to that employed for inspection of polished wafers described
A bright-field image of a wafer area containing a 1 w par- earlier (Section IIIC2). The systemhas the capability to detect,
ticle is shown in Fig. 13(a), where the field size is 175 X 130 identify, and mark the location of single yam flaws for cloth
bm (16X objective) and the focused spot size is approximately widths up to 330 cm at throughput rates of 225 m/min [271.
2 bm. Fig. 13(b) shows the sameimagefieldusing only the The optical portion of this system is illustrated in Fig. 14.
650 PROCEEDINGS OF THE IEEE, VOL. 70, NO. 6 , JUNE 1982
The beam from a He-Ne laser is split by a beamsplitter andthe the size of the incident beam. A number of laser scanning sys-
resulting beams are shaped into mutually orthogonal elliptical tems have been developed and used to elicit a variety of photo-
shapes, each ellipse having a width approximately equalto the electric responses from semiconductor specimens. Photoemis-
distance between the centerlines ofadjacent yarns. The shaped sion [ 381, p-n junction photovoltage [ 391, bulk photovoltage
beams are reflected off a rotating, multifaceted pirror which and photoconductance 1401, and optically induced avalanche
direets the beams onto the woven fabric at a 45 angle to the breakdown [4 1] have all been studied.
direction of fabric flow. One beam is focused so that the long Of note is the fact that somewhat similar mechanisms can be
part of the oval is parallel to the threads aligned in the cloth exploitedfor the inspection of metals. Fatigue damagein
flow direction, and the other beam is oriented with the per- metalshasbeendisplayed and assessedbymeansof photo-
pendicular set of threads. electron emission [42]. The surface of a fatigue sample is
Since the two beams strike the mirror at a 20' angular offset, scanned by a focused UV laser, and the spatial distribution of
and the scan is made in a 20' arc, only one elliptical beam is the electron emission is displayed on an oscilloscope. The local
on the cloth at any given instant. In this manner, warp and intensity of the emission is a quantitative m w u r e of the local
fill thread flaws can ultimately be distinguished. Three sets of fatigue deformation. The fatigue process generates microscopic
fiber-optic bundle detectors collect reflected, direct, and scat- extrusions of fresh metal, from which electrons are photo-
tered laserlight from the fabric. The collected light is con- emitted .more easily than from the surrounding oxide-coated
verted into electrical signals by photomultiplier tubes. In most surface.
cases, for a single scan the defect signal is barely larger than 3) Biomedical Applications: Scanning laser techniques
the background noise and of about the same frequency. Hence have been employed in a number of applications in the area of
the defects are virtually indistinguishable from other areas of biomedical research. Amongthe advantages of laser microscopy
the trace, and undetectable. However,whatmakes these de- is the fact that microbiological objects need not be stained, an
fects objectionable is their persistence. They must persist for important consideration when living cells are to be observed
several centimetersbeforethey can be classified as a flaw. [ 11. A laser scanning systemfor use in automated fluorescence
Therefore, once these signals from the flaw are processed suffi- microscopyhasbeendeveloped [ 151.The systemuses an
ciently that a predetermined threshold can be exceeded, even argon laser (488 nm) and was optimized for use with fluores-
though similar areas ofthe scan may also exceedthe threshold, cein isothiocyanate(FITC) stained samples. FITC is the
this scan is stored in memory and compared to subsequent fluorochrome usedalmostexclusivelyinclinical fluorescent
scans along with information as to the cloth movement. This antibody tests. Another example of an instrument developed
information is used to determine if the flaw is still present in for biomedical microscopy is a Laser Scanning Phase Modula-
subsequent scans,and only when it hasbeen detected often tion Microscope 1431 . In this system a laser flyingspot scanner,
enough to achieve a minimum length is it considered a flaw. a phase modulation servo system, and digital image processing
The signalsare subsequentlypresented to a microprocessor are integrated with a highquality optical research microscope.
which links the information together for flaw identification, The laser is rastered across the specimen with galvanometers,
severity, and location. The location offlaws is indicated by yielding a 51 2 x 512 pixel image with potential resolution to
spraying a washable dye onto the edgeof the cloth. Upon 0.5 pm. The phase modulation system useslead lanthanum
completion of the inspection of a roll of cloth, a high-speed zirconate titanate (PLZT) ceramic electrooptic light modulators
line printer displays a topographical defect map of the roll for in an innovative servo design. When combined with the laser
subsequent analysis. scanner, it permits point-by-point measurement of any one of
2) Semiconductor Material Characterization: Laser scanning several optical properties (birefringence, optical rotation, etc.)
techniques have been extensively reported for the noncontact and thus an image can beformed in several modes.
investigationand evaluation of semiconductor materialsand A laserscanning microscope hasbeen employed to make
circuits [281-[351.Bothelementalsemiconductors, such as high-precisionmeasurementsofcells from clinicalcervical
silicon and germanium, and compound semiconductors, such samples [ M I . The instrument consists of a Leitz fluorescent
as gallium arsenide
and indiumphosphide, have
been microscopewithepi-illumination. Illumination is available
investigated. from three sources; tungsten and mercury lamps, and a He-
Thetechniques developedcanbedivided into twobroad CdIO-mW laser (441 nm). The first two sources are used to
categories [36]. First, those in which the input and output locate and identify the cells; actual scanning measurements
from the sample under investigation are optical. For example, are made with the laser. The incident and emitted light are
the IRtransmittance, reflectance, and photoluminescence separated using a flter and dichroic mirror system t o permit
[37] of semiconductor materials can provide information on illumination below 455 nm andemissionabove 490 nm. A
internal inhomoge,neities, impurity precipitations, photoioniza- 0.5-pm spot is produced inthe plane of the cells and is rastered
tion effects, and inclusions of foreign material. An instrument across the cell with a vibrating mirror from top to bottom in
called the ScannedLaser Infrared Microscope(SLIM)has the field, while the stage (undercomputer control) moves
been developed for such evaluations [ 141. the slide laterally in 0.5-#m steps following the completion
The second category includes those techniques in which an of each laser scan. In this manner, a field 50 pm X 50 pm is
optical input is used to elicit an electrical response. Techniques swept out. The specimens are stained with the DNA specific
in this category rely on the fact that incident optical radiation fluorochromemithramycin, and the fluorescent imagesana-
absorbed within a semiconductor will disturb the equilibrium lyzed to give information on nuclear size, DNA content, and
distribution of carriers and hence the electrical properties of nuclear shape.
the sample. The extent of the modification of the electrical
properties depends not only on the illumination but also on IV. SUMMARY
the properties of the material itself in the illuminated region. In this paper we have presented the basic concepts of laser
In principle then, techniquescould be developed for measuring scanning microscopy, and reviewed various applications of the
the electrical properties of a sample with a resolution set by technique, particularly in the semiconductor industry. Laser
ALFORD e t al.: LASER SCANNING MICROSCOPY 651
microscopy was shown t o offer several unique advantages over hardsurface photolithographic masks via laser photodeposition,”
conventional imaging, and it is hoped that the various applica- IEEE ElectronDevice Lett., vol. EDL-1, no. 6, pp. 101-103,
June 1980.
tions presented demonstrated these concepts. [21] A.W. Warner, D.L. White, and W.A. Bonner,“Acousto+ptic
Therecentintroduction of
several
commercial systems light deflectors using opticalactivity in paratellurite,” J. AppL
P h y s , vol. 43, no. 11, pp. 4 4 8 9 4 4 9 5 , 1 9 7 2 .
employing the principles of laser microscopy serves to illus- (221 D.R. Oswald and D.F. Munro, “A laser scan technique for elec-
trate the potential impact that the technique is predicted to tronic materials surface evaluation,” J. Elect. Mat., vol. 3, no. 1,
have in the future. Improvements in optical system design, pp. 225-242, Feb. 1974.
[23] A. D. Gara, “Automatic microcircuit and wafer hspection,”
the use of shorter wavelengths with the resulting increase in Electron Test, pp. 60-70, May 1981.
resolution, and the continued use of more powerful electronic [24] NASA Tech Briefs, “Automatic inspectionof silicon wafers,”
p. 403, Fall 1979.
signalprocessingandanalysis will extend the applications [25] W. J. Patrick and E. J. Patner, “The detection of surface defects
potential of laserscanningmicroscopy far beyond what is on silicon wafers by scattered light measurements,” in Semicon-
presented in this review. ductor Silicon, by H. R. Huff and R. R. Burgess, Eds. pp. 482-
490, 1973.
I261 R L. Schmitt, “The laser scanning microscope,” to be published.
(271 L. E. Seidel, “Cloth inspection: A better idea,” TextUeZnd., Feb.
REFERENCES 1978.
[28] E. D. Jungbluth and J. F. Black, ‘‘Scanning laser infrared micro-
C.J.R. Sheppard, “The scanning optical microscope,” presented scopy of doping inhomogeneities in I n h single crystals,” Solid
at the IEEE/OSA CLEA Conf., Washington, DC, June 1977. StateCommun.,vol. 13, pp. 1099-1105, 1973.
C. Cremer and T. Cremer,“Considerations on a laser scanning [29] J. N. Gannaway and T.Wilson, “Examination of grain boundaries
microscope with high resolution and depthof tield,”Microsopica in polycrystalline solar cells using a scanning optical microscope,”
Acta, vol. 81, no. 1, pp. 31-44, 1978. Electron Lett.,vol. 14,no. 16, pp. 507-8, Aug. 1978.
D. C. Gupta, B. Sherman et aL, “Non destructure semiconductor [30] E.D. Jungbluth, “Observations of impurity inhomogeneities in
testing using scanned laser techniques,” Solid-stateTechnoL, arsenic-doped silicon by scanning laser microscopy,” AppL Phys
vol. 14, no. 3, pp.. 44-50, Mar. 1971. Lett., vol. 17, no. 10, pp. 437-440, Nov. 1970.
D. E. Sawyer and D. W. Berning, “Thermal mapping of transistors [ 31 ] N. N. Armencha et aL , “Leakage current channels in integrated
with a laser scanner,” R o c . ZEEE, vol. 64, no. 11, pp. 1634-5, circuits using a laser scanning microscope,” Mikros’hktrinikcr,
Nov. 1976. vol. 7, no. 1 , pp. 66-69, Jan.-Feb. 1978.
D. L. Fehrs, D.R. Munro, and J. D. Cuthbert, “Automatic laser [ 321 R. E. McMahon, “Laser tests IC’s with light touch,” Electronics,
inspection/laser repair system for masks,” presented at the IEEE/ vol. 44, no. 8 , pp. 92-95, Apr. 1971.
OSA CLEA Conf., Washington, DC, May 1975. [33] D. L. Lile and N. M. Davis, “Semiconductor profiling using an
J. D. Cuthbert, D. F. Munro, and D. L. Fehrs, “A microelectronic optical pTobe,” Solid-stateElectron, vol. 18, pp. 699-704,
mask inspection systembased on single spot laser scan techniques,” July-Aug. 1975.
Japan J. AppL P h y s , vol. 14, Suppl. 14-1, 1975. ( 3 4 1 P. Shah and L. Crosthwait, “Laser assisted automated surface
J. H. Bruniug et aL, “An automated mask inspection system- analysis in process control of large scale integrated circuits,”
AMIS,” IEEE Trons Electron Devices‘. vol. ED-22, no. 7 , pp. presented at the IEEE/OSA CLEOS Conf., San Diego, CA, 1978.
487-495, July 1975. [35] D. V. Lang and C. H. Henry.“Scanningphotocurrentmicrmopy:
P. Geise et aL, “A laser scanner for PC board inspection.” Elec- A new technique to studyinhomogeneously distributed recombi-
t r o n Packuging Production, vol. 20, no. 12, pp. 152-163, Dec. nation centers in semiconductors,’’ Solidstate Electron,vol. 21,
1980. pp. 1519-1524, Nov.-Dec. 1978.
R.H. Kingston, “Detection of optical and infrared radiation,” in I361 D. L. Lile and N. M. Davis, “Optical techniques for semiconduc-
Sprimer Series in Optical Sciences, D. L. McAdam, Ed. New tor material and circuitinspection,” SolidState TechnoL, vol.
York: Springer, 1978. 18, no. 6, pp. 29-32, July 1975.
J. C. Leader,“Polarizationdependence in EM scattering from [37] J. F. Black et aL, “Scanned4aser microscopy for photolumines-
Rayleigh scatterersembedded in adielectricslab-theory I,” J. cence studies,” AppL Opt., vol. 4 , no. 7 , pp. 1553-1562, July
AppL P h y s , v o l . 46, no. 10, pp. 4371-4385,1975. 1972.
D. P. Jablonowski, unpublished work, 1977. [38] T. H. DiStefano, “Barrier inhomogeneities on a SiSiO, interface
R. E. Frazee and L. S. Watkins, “Gold contact f i g e r inspection by scanning internal photoemission,” AppL Phys. Lett., vol. 19,
using a scanning laser beam,” ZEEE Tmns Znd Electron Contr. no. 8:,pp 280-292, 1971.
Irutrum, vOI. IECI-23, pp. 1-7, 1976. - , &electric breakdown induced by sodium in MOS struc-
D. Kermisch, ‘‘Visualization of large variation phase objects,” tures,” J. AppL P h y s , vol. 4 , no. 1, pp. 527-528, 1973.
Roc. SPIE,vol. 74, pp. 126-129,1976. [39] J. R. Haberer, “Photoresponsemapping of semiconductors,”
D. J. Ketcham,“Real time image enhancement techniques,” Phys. Failure Electron, vol. 5, pp. 5 1 4 2 , 1 9 6 7 .
h c . SPLE, V O ~ .74, pp. 120-125,1976. [40] D.L. Blackburn, H.A. Schafft, and L. J. Swartzendruber, “Non-
B. Sherman and J. F. Black, “Scanned laser infrared micro- destructive photovoltaic technique for the measurement of resis-
scope,” AppL Opt., vol. 9, no. 4 , pp. 802-809,1970. tMty gradients in circular semiconductor wafers,” J. Electrochem.
A. F. Slomba et aL, “A laser flying spot scanner for use in auto- SOC: Solid-StateSci TechnoL,vol. 119, pp. 1773-1778,1972.
matedfluorescence antibodyinstrumentation,” J. A w c . Ad- [41] C.N. Potter and D.E. Sawyer, “Optical scanning techniques for
wncement of Medical Instrum., vol. 6, no. 3, pp. 230-234, May- semiconductor device screening and identification of surface and
June 1972. junction phenomena,” Phys Failure Electron, vol. 5, pp. 37-
D.
P. Jablonowski, “Interference positionmonitoringsystem 50, 1967.
employing ronchi gratings,” Rev. Sci Instrum., vol. 47, no. 1, [42] W.J. Baxter, “Laser scanner for exoelectron display and measure-
pp. 96-100, 1976. melit of fatigue damage,” JTEVA, vol. 5 , no. 4 , pp. 243-250,
K. A. Stetson, “Holographic surface contouring by limited July 1977.
depth of focus,” AppL Opt,, vol. 7 , no. 5, pp. 987-989, 1968. [43] E. W. Hansen et aL, “Laser scanning phase modulation micro-
R.W. Berry et aL, Thin Film Technology. New York: D. Van scope,” presented at the OSA Annual Meet., Oriando, FL, Oct.
Nostrand, 1968. 1981.
R. A. Kaplan, “A microprocessor-controledmask inspection and [44] P. J. Mullaney et aL, Models for Low Resolution S l f t Scan Mea-
repair system,” SolidState TechnoL, vol. 17, no. 4, pp. 74-78, surements Based on High Resolution Laser Scanning Image
Apr. 1976. Analya: DNA and Nuclear Dimensions, PatternRecognition,
D. J. Ehdich et aL. “One-step repair of transparent defects in Elmsford, NY: Pergamon, 1981.~01.13, pp. 49-55.