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Animal Micros

This document provides instructions for a laboratory activity introducing students to animal microscopy through examining prepared slides of invertebrate and vertebrate tissues under a microscope. Students are tasked with classifying specimens by phylum based on observable features, drawing and labeling tissue samples to identify primary tissue types and their functions. The activity aims to familiarize students with microscopy terminology and techniques used in the field of zoology to study animal structures and classify organisms.

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31 views

Animal Micros

This document provides instructions for a laboratory activity introducing students to animal microscopy through examining prepared slides of invertebrate and vertebrate tissues under a microscope. Students are tasked with classifying specimens by phylum based on observable features, drawing and labeling tissue samples to identify primary tissue types and their functions. The activity aims to familiarize students with microscopy terminology and techniques used in the field of zoology to study animal structures and classify organisms.

Uploaded by

espantocd
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as PDF, TXT or read online on Scribd
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Republic of the Philippines

Department of Science and Technology


PHILIPPINE SCIENCE HIGH SCHOOL - Ilocos Region Campus
San Ildefonso, 2728 Ilocos Sur

Name: Date:
Section/Group Code: Score:

INTEGRATED SCIENCE 2 SY 2022-2023


LABORATORY ACTIVITY 1.3: AN OVERVIEW OF ANIMAL MICROSCOPY

INTRODUCTION
Aristotle is known as the Father of Zoology (the study of animals) due to his exceptional work in one of the earliest
systematic organization and grouping of animals. Today, zoology has given insights and contributions in other fields
such as in ecology, genetics, evolution, medicine, and human anatomy, among others.
A significant work in zoology is examining animals under the microscope. With this activity, you are expected to have
an overview of how microscopy is utilized in this field.

OBJECTIVES
In this activity, you are expected to:
1. be familiarized with the common terminologies used in animal microscopy;
2. observe and identify animal tissues (from prepared slides) using an electric microscope; and
3. explain how the tissues structures are related to their functions.

MATERIALS
microscope, prepared slides for zoology, cedarwood oil

PART I: DIRECTIONAL/DIMENSIONAL TERMINOLOGIES

Have you ever wondered how an animal (or a part of


it) is processed to be placed in the microscope slides that
you are about to examine today? In a nutshell, the
sample undergoes a process called fixation where it is
immersed in chemicals that preserve the features of the
tissue as if they are still alive. Then, if only a tissue of the
organism is to be examined, the sample is cut very
carefully using specialized equipment such as a
microtome. After a few more processes, the sample
undergoes staining, which then enhances the features
of the sample by providing it with color and contrast.
Acquiring the right “slice” of the sample is not as
easy as you might think! If this procedure is performed
incorrectly, it may render the sample entirely unusable.
Deciding how to process the sample requires you to
apply spatial and dimensional thinking. For example,
depending on what part should be examined under the
microscope, a sample can be cut in many different
planes (transverse, frontal, midsagittal), producing
different sections.
Note that tiny specimens can also be examined as a
whole, called a whole mount (w.m.).

Figure 1. Planes and sections of the human body. The transverse plane produces a transverse section (also called cross-section
(c.s.)); the frontal plane produces a frontal section (also called coronal section); and the sagittal plane produces the sagittal
section (also called longitudinal section (l.s.)). From “Planes of Body” by Connexion. CC BY 4.0.
ASSESSMENT/OBSERVATION SCORE: _______

Instructions: Identify the correct plane used as reference in each of the images on the section of the brain (1 point
each). Write your answer on the space provided below each image.

Figure 2. Isometric image of the


brain. Modified from “Blausen 0104
Brain x-secs SectionalPlanes” by
Bruce Blaus. CC BY-SA.

PART II: EXAMINATION OF PREPARED SLIDES FOR ZOOLOGY

A. INVERTEBRATES

Invertebrates are animals characterized by the lack of a developed vertebral column. They are among the simplest
animals but they comprise around 99% of animal species. As a result, they are highly diverse, and using microscopy
enables the identification and classification of these organisms.

ASSESSMENT/OBSERVATION SCORE: _______

Instructions: Select three (3) prepared slides of invertebrates and examine each under the microscope. Classify
each specimen into its correct invertebrate phylum (1 point each). Then, using the appropriate magnification, draw
the specimens and indicate/label at least one (1) feature/structure that characterizes its phylum (2 points each for
the correct drawing; 1 point each for the correctly indicated feature/structure).

Slide 1 Name: Slide 2 Name: Slide 3 Name:

Magnification: Magnification: Magnification:

Phylum: Phylum: Phylum:


B. VERTEBRATES

Vertebrates are animals that have backbone. They are relatively more complex than invertebrates and have
developed more specialized tissues. Tissues in animals can be classified into four primary types: epithelial,
connective, muscle, and nervous tissues. In vertebrates, examination of tissue samples not only enables
taxonomic classification, but also allows further study on how they work and what they are made of.

ASSESSMENT/OBSERVATION SCORE:
_______

Instructions: Select three (3) prepared slides with different primary tissue types and examine each under the
microscope. Then, using the appropriate magnification, draw the specimens and indicate/label at least one (1)
feature/structure that correlates to its intended function (2 points each for the correct drawing; 1 point each for
the correctly indicated feature/structure; 2 points for the correct function).

Slide 1 Name: Slide 2 Name: Slide 3 Name:

Tissue Type: Tissue Type: Tissue Type:

Magnification: Magnification: Magnification:

Function of Specified Function of Specified Function of Specified


Tissue/Feature/Structure: Tissue/Feature/Structure: Tissue/Feature/Structure:

SYNTHESIS/REFLECTION

Instruction: Write a reflection on your insights and learnings on this activity (3 points for substantial content, 2
points for clarity and organization of ideas).

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