BIRLA INSTITUTE OF TECHNOLOGY AND SCIENCE, PILANI-HYDERABAD CAMPUS

BIOF111: GENERAL BIOLOGY FIRST SEMESTER 2023-24

Announced QUIZ-2; Date: 31.10.23; Total marks: 45 M
Duration: 30 minutes (14:10-14.40). Encircle the correct option with a pen. Multiple encircling
will be considered incorrect. For changing selected option take examiner’s signature.
Name: STUDENT-ID: Tutorial Section No:
Please choose the most appropriate option for each question.

1. Dark-green color of leaves is dominant over yellowish-green in rice. What is the percentage of plants
with green-colored leaves if the homozygous plants with the two phenotypes are crossed?
a. 100
b. 50
c. 25
d. 75
2. In a family, the father has sickle cell anemia (ss), a recessive disorder. If the mother is heterozygous
for the mutant allele (Ss), the fraction of children predicted to have sickle cell anemia would be:
a. 1/2
b. 1/4
c. 1/6
d. 1/8
3. Suppose you wish to create a large batch of the protein lactase using recombinant DNA technology.
Place the following steps in the order you would have to perform them.
I. Find the clone with the gene for lactase
II. Insert the plasmids into bacteria and grow the bacteria into clones
III. Isolate the gene for lactase
IV. Create recombinant plasmids, including one that carries the gene for lactase
a. III, IV, II, I
b. I, II, III, IV
c. II, IV, I, III
d. IV, III, II, I
4. The term “DNA fingerprint” is used to imply that an individual’s DNA differs from the DNA of
others. In what way is a person’s DNA unique?
a. Everyone has a different number of chromosomes
b. Everyone has a different number of genes
c. Everyone has a different number of short tandem repeats
d. All of the above are correct
5. How many double-stranded DNA molecules are obtained from one double-stranded DNA molecule
after 5 cycles of PCR?
a. 8
b. 64
c. 32
d. 16
6. A technique commonly used to separate DNA fragments of varying lengths is ______.
a. Electrophoresis
b. DNA sequencing
c. Polymerase Chain Reaction
d. DNA fingerprinting
7. The DNA fingerprint of a child is ________.
a. 100% similar to the DNA fingerprint of both the parents
b. 100% similar to the brother’s DNA fingerprint
c. 100% similar to the sister’s DNA fingerprint
d. 50% of the DNA fingerprint similar to the father and remaining 50% similar to the
mother
8. Identify the incorrect statement from the given below:
a. E. coli can accommodate chromosomal DNA and plasmid DNA in its cytoplasm
b. Plasmid DNA is linear in nature
c. Producing identical copies of target DNA molecule is part of cloning
d. Plasmids are a form of vectors
9. Assuming that you are given a circular double stranded DNA with unevenly spaced 4 restriction sites
for “X” restriction enzyme. After the digestion with enzyme “X”, you expect to observe ___________
(number) of DNA fragments in agarose gel electrophoresis.
a. 2
b. 4
c. 5
d. 6
10. A DNA molecule migrates from _________ to ____________ under the influence of electric field.
a. Anode to cathode
b. Cathode to anode
c. Does not migrate
d. Depends on the percentage of agarose in the gel electrophoresis
11. The given PCR reaction mixture contains dNTPs, buffer, template DNA (200ng), primers and heat
sensitive DNA polymerase. What will be the amount of DNA synthesized by the end of 10 cycles of
PCR reaction?
a. 202800ng
b. 102400ng
c. 0 ng
d. 25600ng
12. Which of the following statements is incorrect?
a. Alleles are alternate forms of a gene
b. Alleles of a gene occupy different positions (locus) on homologous chromosomes
c. More than two alleles can exist for a gene in a population
d. None of the above is incorrect
13. Assume that a gene has two alleles ‘A’ and ‘a’. Which of the following correctly represents
genotypic ratio for Aa x Aa cross (monohybrid) ?
a. 2AA:1Aa:1aa
b. 1AA:2aa:1Aa
c. 1AA:1aa:1Aa
d. None of the above
14. A plant heterozygous for two genes (AaBb) when undergoes meiosis produces gametes. Which of the
following is correct about the gametes produced?
a. Four types of gametes with allelic combinations: ‘AA’ or ‘aa’ or ‘BB’ or ‘bb’
b. Two types of gametes with allelic combinations: ‘Ab’ or ‘aB’
c. Two types of gametes with allelic combinations: ‘AB’ or ‘ab’
d. Four types of gametes with allelic combinations: ‘AB’ or ‘Ab’ or ‘aB’ or ‘ab’
15. If the blood groups of the parents are O and AB, then the possible blood groups of their children would
be:
a. AB and O
b. A, B, O, and AB
c. A and B
d. A, B, and O

16. Color blindness is an X-linked recessive trait. If a female with color blindness (XcXc) marries a normal
male (XY), then which of the following will be true?
a. All their sons will be color blind
b. All their daughters will be color blind
c. All their sons and daughters will be color blind
d. 50% their sons and daughters will be color blind
17. A typical human somatic cell has _________ number of chromosomes in total, of which
____________are autosomes and _____are sex chromosomes.
a. 2, 44, 46
b. 23, 44, 46
c. 46, 44, 2
d. 2, 2, 23
18. Prophase-1 of meiosis is different from prophase of mitosis with respect to
a. Crossing over and synapsis of chromosomes
b. Absence of spindle
c. Having 92 chromatids
d. Breakdown of nuclear envelope
19. Which of the following events can drive transformation of a proto-oncogene into an oncogene?
a. Mutation within the gene
b. Gene moved to a new DNA position under new controls
c. Gene duplication event creating multiple copies of the gene
d. All of the above
20. Cancers take a long time to develop because:
a. It takes a long time for cells to grow and divide
b. Young cells are not susceptible to DNA mutations
c. Multiple DNA mutations must accumulate in the same cell for it to become cancerous
d. Many generations of cancerous cells must occur before the DNA will mutate

21. A nonsense mutation in a tumor-suppressor gene most likely will ___________.

a. cause uncontrolled cell growth
b. cure cancer
c. inhibit cell division
d. Both b and c

22. The interphase period before and after S phase are called __________ and _________ respectively.
a. G0 and Gα
b. G0 and G20
c. G1 and G2
d. G123 and G012
23. Paclitaxel is a chemotherapeutic drug that a) Impacts mitotic spindle and prevents cell division; b) Is
discovered from bark of Pacific yew c) Freezes the spindle after it forms. Which of the statements
given above are true?
a. Only a and b
b. All three
c. Only b and c
d. Only a and c
24. Which of these is not a component contributing to genetic variation in individuals?
a. Crossing over
b. Mutation
c. Fertilization
d. Cytokinesis
25. Klinefelter syndrome in male can be a result of which type of nondisjunction
a. Autosomal non-disjunction
b. Extra X chromosome in male like XXY, XXXY, XXXXY etc…
c. XO
d. Trisomy 21.
26. Chromosomes of many animals contain healthy genes that can become cancer causing oncogenes.
They are called as
a. Triple oncogenes
b. Protooncogenes
c. Alphaoncogenes
d. Kleptooncogenes.
27. Birds of a certain species possess either black, white or grey feathers. These colors are imparted by a
single locus gene containing 2 alleles. These alleles most likely exhibit:
a. Complete dominance
b. Incomplete dominance
c. Co-dominance
d. Cannot be deduced
28. In a certain species of plant, flower colour is encoded by a single locus gene containing 2 alleles.
The petals are either-red, white or red with white spots. These alleles thus exhibit:
a. Complete dominance
b. Incomplete dominance
c. Co-dominance
d. Cannot be deduced
29. A tall mango plant (TT) producing big mangoes (Bb) is crossed with a dwarf mango plant (tt)
producing small mangoes. T is dominant over t and B over b. What percentage of the progeny will
be dwarf and produce big mangoes?
a. 25
b. 50
c. 75
d. 0
30. A normal female who is a carrier for hemophilia (X-linked) marries a hemophilic male. The
percentage of progeny that will be hemophilic male is:
a. 25
b. 50
c. 75
d. 100
 BIRLA INSTITUTE OF TECHNOLOGY AND SCIENCE, PILANI-HYDERABAD CAMPUS
BIOF111: GENERAL BIOLOGY FIRST SEMESTER 2023-24
Announced QUIZ-2; Date: 31.10.23; Total marks: 45 M
Duration: 30 minutes (14:10-14.40). Encircle the correct option with a pen. Multiple encircling
will be considered incorrect. For changing selected option take examiner’s signature.
Name: STUDENT-ID: Tutorial Section No:
Please choose the most appropriate option for each question.

1. Identify the incorrect statement from the given below:

a. E. coli can accommodate chromosomal DNA and plasmid DNA in its cytoplasm
b. Plasmid DNA is linear in nature
c. Producing identical copies of target DNA molecule is part of cloning
d. Plasmids are a form of vectors
2. Assuming that you are given a circular double stranded DNA with unevenly spaced 4 restriction sites
for “X” restriction enzyme. After the digestion with enzyme “X”, you expect to observe ___________
(number) of DNA fragments in agarose gel electrophoresis.
a. 2
b. 4
c. 5
d. 6
3. A DNA molecule migrates from _________ to ____________ under the influence of electric field.
a. Anode to cathode
b. Cathode to anode
c. Does not migrate
d. Depends on the percentage of agarose in the gel electrophoresis
4. The DNA fingerprint of a child is ________.
a. 100% similar to the DNA fingerprint of both the parents
b. 100% similar to the brother’s DNA fingerprint
c. 100% similar to the sister’s DNA fingerprint
d. 50% of the DNA fingerprint similar to the father and remaining 50% similar to the
mother
5. Dark-green color of leaves is dominant over yellowish-green in rice. What is the percentage of plants
with green-colored leaves if the homozygous plants with the two phenotypes are crossed?
a. 100
b. 50
c. 25
d. 75
6. Suppose you wish to create a large batch of the protein lactase using recombinant DNA technology.
Place the following steps in the order you would have to perform them.
I. Find the clone with the gene for lactase
II. Insert the plasmids into bacteria and grow the bacteria into clones
III. Isolate the gene for lactase
IV. Create recombinant plasmids, including one that carries the gene for lactase
a. III, IV, II, I
b. I, II, III, IV
c. II, IV, I, III
d. IV, III, II, I
 7. In a family, the father has sickle cell anemia (ss), a recessive disorder. If the mother is heterozygous
for the mutant allele (Ss), the fraction of children predicted to have sickle cell anemia would be:
a. 1/2
b. 1/4
c. 1/6
d. 1/8
8. The term “DNA fingerprint” is used to imply that an individual’s DNA differs from the DNA of
others. In what way is a person’s DNA unique?
a. Everyone has a different number of chromosomes
b. Everyone has a different number of genes
c. Everyone has a different number of short tandem repeats
d. All of the above are correct
9. How many double-stranded DNA molecules are obtained from one double-stranded DNA molecule
after 5 cycles of PCR?
a. 8
b. 64
c. 32
d. 16
10.A technique commonly used to separate DNA fragments of varying lengths is ______.
a. Electrophoresis
b. DNA sequencing
c. Polymerase Chain Reaction
d. DNA fingerprinting
11. The given PCR reaction mixture contains dNTPs, buffer, template DNA (200ng), primers and heat
sensitive DNA polymerase. What will be the amount of DNA synthesized by the end of 10 cycles of
PCR reaction?
a. 202800ng
b. 102400ng
c. 0 ng
d. 25600ng
12. Which of the following statements is incorrect?
a. Alleles are alternate forms of a gene
b. Alleles of a gene occupy different positions (locus) on homologous chromosomes
c. More than two alleles can exist for a gene in a population
d. None of the above is incorrect
13. Assume that a gene has two alleles ‘A’ and ‘a’. Which of the following correctly represents
genotypic ratio for Aa x Aa cross (monohybrid) ?
a. 2AA:1Aa:1aa
b. 1AA:2aa:1Aa
c. 1AA:1aa:1Aa
d. None of the above
14. A plant heterozygous for two genes (AaBb) when undergoes meiosis produces gametes. Which of the
following is correct about the gametes produced?
a. Four types of gametes with allelic combinations: ‘AA’ or ‘aa’ or ‘BB’ or ‘bb’
b. Two types of gametes with allelic combinations: ‘Ab’ or ‘aB’
c. Two types of gametes with allelic combinations: ‘AB’ or ‘ab’
d. Four types of gametes with allelic combinations: ‘AB’ or ‘Ab’ or ‘aB’ or ‘ab’
15. If the blood groups of the parents are O and AB, then the possible blood groups of their children would
be:
a. AB and O
b. A, B, O, and AB
c. A and B
d. A, B, and O

16. Color blindness is an X-linked recessive trait. If a female with color blindness (XcXc) marries a normal
male (XY), then which of the following will be true?
a. All their sons will be color blind
b. All their daughters will be color blind
c. All their sons and daughters will be color blind
d. 50% their sons and daughters will be color blind

17. A typical human somatic cell has _________ number of chromosomes in total, of which
____________are autosomes and _____are sex chromosomes.
a. 2, 44, 46
b. 23, 44, 46
c. 46, 44, 2
d. 2, 2, 23
18. Prophase-1 of meiosis is different from prophase of mitosis with respect to
a. Crossing over and synapsis of chromosomes
b. Absence of spindle
c. Having 92 chromatids
d. Breakdown of nuclear envelope
19. Which of the following events can drive transformation of a proto-oncogene into an oncogene?
a. Mutation within the gene
b. Gene moved to a new DNA position under new controls
c. Gene duplication event creating multiple copies of the gene
d. All of the above
20. Cancers take a long time to develop because:
a. It takes a long time for cells to grow and divide
b. Young cells are not susceptible to DNA mutations
c. Multiple DNA mutations must accumulate in the same cell for it to become cancerous
d. Many generations of cancerous cells must occur before the DNA will mutate

21. A nonsense mutation in a tumor-suppressor gene most likely will ___________.

a. cause uncontrolled cell growth
b. cure cancer
c. inhibit cell division
d. Both b and c

22. The interphase period before and after S phase are called __________ and _________ respectively.
a. G0 and Gα
b. G0 and G20
c. G1 and G2
d. G123 and G012
23. Birds of a certain species possess either black, white or grey feathers. These colors are imparted by a
single locus gene containing 2 alleles. These alleles most likely exhibit:
a. Complete dominance
b. Incomplete dominance
c. Co-dominance
d. Cannot be deduced
24. In a certain species of plant, flower colour is encoded by a single locus gene containing 2 alleles.
The petals are either-red, white or red with white spots. These alleles thus exhibit:
a. Complete dominance
b. Incomplete dominance
c. Co-dominance
d. Cannot be deduced
25. A tall mango plant (TT) producing big mangoes (Bb) is crossed with a dwarf mango plant (tt)
producing small mangoes. T is dominant over t and B over b. What percentage of the progeny will
be dwarf and produce big mangoes?
a. 25
b. 50
c. 75
d. 0
26. A normal female who is a carrier for hemophilia (X-linked) marries a hemophilic male. The
percentage of progeny that will be hemophilic male is:
a. 25
b. 50
c. 75
d. 100
27. Paclitaxel is a chemotherapeutic drug that a) Impacts mitotic spindle and prevents cell division; b) Is
discovered from bark of Pacific yew c) Freezes the spindle after it forms. Which of the statements
given above are true?
a. Only a and b
b. All three
c. Only b and c
d. Only a and c
28. Which of these is not a component contributing to genetic variation in individuals?
a. Crossing over
b. Mutation
c. Fertilization
d. Cytokinesis
29. Klinefelter syndrome in male can be a result of which type of nondisjunction
a. Autosomal non-disjunction
b. Extra X chromosome in male like XXY, XXXY, XXXXY etc…
c. XO
d. Trisomy 21.
30. Chromosomes of many animals contain healthy genes that can become cancer causing oncogenes.
They are called as
a. Triple oncogenes
b. Protooncogenes
c. Alphaoncogenes
e. Kleptooncogenes.