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Assessment 1 Biochemistry

1) The document reports on anthropometric and biochemical data collected from a class, including body fat percentage, BMI, BMR, blood pressure, and salivary amylase activity. 2) Key findings include the class average BMI of 27.7 exceeding the normal range, average BMR aligning with norms, and average blood pressure within normal limits. 3) Salivary amylase activity significantly increased for all subjects after intense exercise, signaling improved glucose metabolism to meet higher energy demands during exertion.

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0% found this document useful (0 votes)
13 views12 pages

Assessment 1 Biochemistry

1) The document reports on anthropometric and biochemical data collected from a class, including body fat percentage, BMI, BMR, blood pressure, and salivary amylase activity. 2) Key findings include the class average BMI of 27.7 exceeding the normal range, average BMR aligning with norms, and average blood pressure within normal limits. 3) Salivary amylase activity significantly increased for all subjects after intense exercise, signaling improved glucose metabolism to meet higher energy demands during exertion.

Uploaded by

cbsj78m27b
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as PDF, TXT or read online on Scribd
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MD5017 Assessment 1: Anthropometric data and

Amylase activity data analysis and interpretation

J Number: J101267
Personal Data Information

If you are unable to complete some of the Personal Data Information, please
leave them blank you will not be deducted any marks. Please use the
information found in your Practical Guide to complete this section.

Sex (M/F) M Age (years)19

180CM Weight (kg) 87

Measurements of Percentage body fat

Estimate of % Body fat calculated from body fat formulas

Men

Factor 1 301.48

Factor 2 162.68

Lean Body Mass (kg) 63.02KG

Body Fat Weight (kg)23.98KG

% Body Fat 27.57

Using the formulas given in your practical guide earlier calculate your:

BMI 27
BMR (calories) 2028

What is your level of lifestyle activity? MODERATE

Calculate optimal calorie intake for your lifestyle activity 3144.49

Class Analysis & Comprehension

To complete this section please use the Excel file containing the student
database collected during the practical session, which can be found on
Moodle under the Assessment tile, below Assessment 1.

On this file you will find data on the following:

1. student number
2. sex
3. % body fat (calculated for the formula)
4. % body fat (from skin fold measurements)
5. BMI
6. BMR (calories)
7. salivary amylase activity
8. Diastolic BP (mmHg)
Section 1
Using the data for the whole class calculate the mean or average values and
standard deviations (Mean ± SD) for:
(this can be done using the statistics functions on Excel or a software of
your choice)

Mean % body fat (formula) MEAN= 22.9 SD=568

Mean % body fat (skinfold) 21.3 SD 6.72

Mean BMI 27.7 SD 9.31

Mean BMR (cal) 1754.40 SD 341.1

Mean Diastolic BP (mmHg) 74.5 SD. 8.72

Mean Salivary amylase activity (µmol/s/L) 28.8 SD. 24.9

1) Briefly comment on the mean values calculated for any three of


parameters of the class and how they compare with the general
population? (no more than 150 words)

Body Mass Index (BMI) is a statistical gauge of body fat, computed based
on height and weight for individuals of any age (Weir, 2023). The typical
BMI for 'normal weight' falls between 18.5 and 24.9 kg/m^2. The class
average BMI of 27.7 slightly exceeds this norm.
Basal Metabolic Rate (BMR) denotes the calories expended during vital
life-sustaining functions (Breitowich, 2023). Women average around 1400
calories, men about 1700. The class average BMR, at 1754.4, aligns with
the norm, encompassing both genders.

Diastolic pressure, the pressure between heartbeats at rest, is considered


normal below 80 mmHg (Solan, 2022). The class average of 74.5 aligns
with this norm, reflecting a healthy diastolic blood pressure range.

Section 2
Plot a relationship graph (scatter plot) and calculate the correlation
coefficient between the two methods. How do the two ways of measuring
% body fat compare (50 words max)?

The correlation coefficient = 0.04055

The results of the two approaches to calculating body fat percentage—using


the formula and skinfold measurements—vary. Although the goal of both
approaches is to determine the percentage of body fat, variations in the
methods used and the underlying assumptions they are based on may cause
discrepancies in the outcomes, indicating the significance of taking
measurement methodology into account when interpreting body fat
percentage values.
Section 3

Group the class data for the BMI, Diastolic BP and salivary amylase activity into
appropriate sets and plot frequency histograms for each (Include the three bar
graphs to your report. This can be done using Excel or on graph paper and
embedded within the document).
(Example: for Diastolic BP it might be the number of students with values ranging from: (set1) 60-
64mmHg, (set2) 65-69, (set3) 70-74, (set4) 75-79, (set5) 80-84, (set6) 85-89, (set7) 90-94, (set8)
95-99. N.B. make sure that the sets have the same range (ie in this case 5mmHg / set).
1. Graph of BMI

Sets

2. Graph of diastolic BP
3. Graph Salivary amylase activity
Section 3A
How do the 3 frequency bar graphs for the physiological / biochemical class
parameters compare? Try to explain the profiles and any anomalies. (no
more 300 words)

The BMI histogram illustrates a prevalent frequency within the 20-30


category, specifically clustered around the typical BMI of 25, signifying a
typical BMI distribution. Instances of BMI surpassing 30 and entering the 55
category were infrequent. In the diastolic BP histogram, the zenith at 70-80
suggests a majority with normal diastolic BP. Few exhibited elevated values
(85-95), while some had lower readings (60-65), still within the normal
range. Salivary amylase activity peaked at 15 (umol/s/L) during rest,
contrasting with diverse post-exercise levels ranging from 5 to 70
(umol/s/L). The varied post-exercise salivary amylase production
underscores pronounced individual differences.
Section 4

Effects of vigorous exercise on salivary amylase activity

In the table below you are given the activity of amylase from four volunteers
who collected their saliva at rest and following 10 minutes of strenuous
activity on an exercise bike.

Volunteers Resting Salivary Salivary Amylase


amylase activity activity after 10min of
(µmol/s/L) Strenuous Exercise
(µmol/s/L)
Individual A 816 1323

Individual B 478 765

Individual C 219 259

Individual D 323 455

a. How do the activities of salivary amylase change after vigorous


exercise? Qualify your answer using appropriate statistical analysis. (100
words max)

Following intense exercise, salivary amylase activity significantly increased in all


subjects. A statistically significant difference (p < 0.05) has been found between
the resting and post-exercise amylase activity using a paired-sample t-test. This
implies that salivary amylase levels are significantly elevated after engaging in
severe activity. The physiological reaction to higher energy needs during exercise
is most likely reflected in the changes that have been seen. The results underscore
the variable character of salivary amylase activity in reaction to physical effort,
underscoring its possible function in metabolic processes both during and
following intense exercise sessions.
b. Try to explain the physiological / biochemical significance of these
results (100 words max).

Post-exercise, heightened salivary amylase activity signals improved glucose metabolism,


vital for increased energy demands during vigorous exercise. This surge in amylase aids
in breaking down saliva's complex carbohydrates, providing an instant energy boost. This
adaptive response supports efficient energy utilization, meeting elevated metabolic
needs during physical exertion. The elevated salivary amylase serves as a biochemical
marker, showcasing the dynamic interplay between enzyme release and metabolic
demands crucial in regulating energy balance during strenuous exercise.

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