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ISO Nitrite in Meat

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56 views4 pages

ISO Nitrite in Meat

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ANTONIS
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TSOP INTERNATIONAL STANDARD Meat and meat products — Determination of nitrite content (Reference method) Viandes et produits 4 base de viande — Détermination de la tencur en nitrites (Méthode de référence) First edition — 1975-09-01 or B UDC 637.5: 546.17 Ref. No, 1S0 2918-1975 (E) Deseiptors: mest, mat products, chemical analysis, 8 Price based on 3 pases mb Kane 2047867 9260417008 20067-0418: jon-Download-Beuth-SPx Process Equipe. INTERNATIONAL STANDARD 180 2918-1976 (E) Meat and meat products — Determination of nitrite content (Reference method) 1 SCOPE AND FIELD OF APPLICATION This International Standard specifies a reference method for the determination of the nitrite content of meat and mest produots. 2 REFERENCE 180 3100, Meat and meat products ~ Sampling. 3. DEFINITION nitrite content of meat and meat products : The nitr Content determined according to the procedure described in this International Standard and expressed as milligrams of sodium nitrite per kilogram (parts per million) 4 PRINCIPLE Extraction of a test portion with hot water, precipitation of the proteins and filtration. In the presence of nitrite, development of @ red colour by the addition. of fulphanilamide and W-1-naphthylethylenedismine dihydro. chloride to the filtrate and photometric measurement st ' wavelength of 538 nm, 5 REAGENTS All reagents shall be of analytical quality. The water used shall be distilled water or water of at least equivalent purity. 5.1. Solutions for precipitation of proteins 5.1.1 Reagent 1 Dissolve 1069 of potassium ferrocyanide trihydrate [KaFe(CN)g-3H20} in water and dilute to 1 000 mi 6.1.2 Reagent It Disolve 2209 of zine acetate dihydrate [Zn(CHsCOO),-2H,0] and 30 mi of glacial acetic seid in water and difute to 1 000 mi 61.3 Borax solution, saturated Dissolve 504 of disodium tetraborate decehydrate (N22B407-10H20) in 1 000 mi of tepid water and ooo! ta ‘00m temperature, 5.2. Sodium nitrite standard solutions Dissolve 1,000 g of sodium nitrite (NaNO2) in water and gilute to 100 mI in one-mark volumetric flask. Pipette SmI of the solution into a 1 000 ml one-mark volumetric flask, Dilute to the mark. Prepare a series of standard solutions by pipetting 5 mi, 10ml and 20 mi of this solution into 100'mi one-mark volumetric flasks and diluting to the mark with water These standard solutions contain respectively 28 ug, 5010p and 10,0 ug of sodium nitrite per miliitre, ‘The ‘standard solutions and the dilute (0,05 g/l) sodium nitrite solution from which they are prepared shall be made up on the day of use, 5.3 Solutions necessary for colour development 8.3.1 Solution | Dissolve, by heating on a water bath, 2g of sulphanilamide (NH2CsH«SOgNH2) in 800m! of water. Cool, filter, if necessary, and add 100 mi of concentrated hydrochlorie acid solution (p29 1,19 g/mil, while stirring. Ditute to 1.000 mi with water. 5.32 Solution 11 Dissolve 0,25 9 of (W-1-naphthylethylenediamine dinydro- Shloride (CyHy NHCHCHNHa-2HCI) in water. Dilute to 250 mi with water Store the solution in a wel-stoppered brown bottle. It shall be kept in a refrigerator, for not longer than one week 53.3. Solution 111 Dilute 445 mi of concentrated hydrochloric acid solution (220 1.19 g/mi) to 1.000 mi with water 1n-Downlond-Beuth-SPX Process Eqvinment Bran + Luebive GrnbH-K

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