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Aula BMol 1

This document provides information about the Molecular Biotechnology course for 2018/2019, including objectives, theoretical classes, laboratory courses, student evaluation, and bibliography. The course aims to teach concepts and tools of recombinant DNA technology and other molecular approaches. Theoretical classes cover topics such as recombinant DNA technology, genetic manipulation applications and ethics. Laboratory courses involve techniques like PCR, bacterial transformation, and Southern blotting. Students are evaluated through tests and practical reports. Recommended reading materials are also listed.

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Diogo Madaleno
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20 views

Aula BMol 1

This document provides information about the Molecular Biotechnology course for 2018/2019, including objectives, theoretical classes, laboratory courses, student evaluation, and bibliography. The course aims to teach concepts and tools of recombinant DNA technology and other molecular approaches. Theoretical classes cover topics such as recombinant DNA technology, genetic manipulation applications and ethics. Laboratory courses involve techniques like PCR, bacterial transformation, and Southern blotting. Students are evaluated through tests and practical reports. Recommended reading materials are also listed.

Uploaded by

Diogo Madaleno
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
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MOLECULAR BIOTECHNOLOGY

2018/2019

Lecturer: Leonilde Moreira


[email protected]

Office: Torre Sul, Piso 6 (6.6.20)

Lab lecturer´s: Leonilde Moreira


Cristina Viegas
Nuno Mira
Filipa Mendes
Rodrigo Costa
♦ Objectives

- Learn concepts and tools of rDNA technology in vitro and other


molecular approaches

- Use of computational tools to explore biological data available at


different databases in order to help scientists to conduct their
research

- With the available techniques, plan strategies to develop


research in a specific topic
♦ Theoretical classes

- Recombinant DNA technology in vitro

- Other molecular approches and its applications

- Molecular Biotechnology in the post-genomic era

- Genetic manipulation of plants and animals. Applications, social


impact and ethics

- Invited seminars
Laboratory course

♦ (Lab1) In silico analyses of nucleotide and amino acid


sequences (Report 1)
DNA amplification by PCR

♦ (Lab2) Introduction of rDNA into bacteria by triparental


conjugation and electrotransformation (Report 2)

♦ (Lab3) Experimental design- I

♦ (Lab4 and Lab5) Southern hybridization (Report 3)

♦ (Lab6) Experimental design- II


Student´s evaluation

♦ Two tests (minimum: 9.0 points in each) (70%) (November


10th and December 17th)

or

♦ Final exam (minimum: 9.0 points) (70%)

♦ Practical course with written reports (30%)


Bibliography

♦ Gene Cloning & DNA analysis: An introduction


T.A. Brown
6th ed., Blackwell Publishing, 2010

♦ Molecular Biotechnology: principles and applications of recombinant DNA. Glick,


Pasternak and Patten, ASM Press, 4th edition, 2010

♦ recommended papers
DNA transformation

Griffith (1928) experiment to demonstrate


Genetic transformation in Streptococcus pneumoniae
Biotechnology- industrial production of goods and services
by processes using biological organisms, systems and processes

Raw material

Principal steps of a
bioengineered biotechnology
Upstream processing
process

Aim: maximize the efficiency


of each step
Fermentation and biotransformation
....if it involves the
manipulation of genes is called
Molecular Biotechnology
Dowstream processing

Pure product
Diverse Fields Contribute to
Molecular Biotechnology
Potential Benefits From
Biotechnology

• More accurate disease diagnosis and treatment


• Improve crop yield, disease and stress
resistance
• Microbial production of chemicals, enzymes,
food additives, pharmaceuticals, polymers, etc.
• Livestock with enhanced genetic attributes
• Elimination of pollutants and waste materials
Potential Social Concerns and
Consequences of Biotechnology

The Farm, by Alexis Rockman


Potential Social Concerns and
Consequences of Biotechnology

• Manipulated organisms harmful to other organisms or


environment
• Manipulated organisms reduce natural genetic
diversity
• Genetic engineering of humans?
• Genetic privacy issues
• Limited availability to poorer nations
• Quest for profit undermines free exchange of
knowledge
Molecular Cloning

What is gene cloning?


The basic steps in a gene cloning experiment are:

1) A fragment of DNA containing the gene to be cloned, is inserted


into a circular DNA molecule called a vector, to produce a
recombinant DNA molecule
2) The recombinat DNA molecule is transported into a host cell

3/4) It multiplies, producing numerous identical copies

5) After a large number of cell


divisions, a colony, or clone, of
identical cells is produced. The
gene carried by the
recombinant molecule is now
said to be cloned

E. coli is the organism


commonly used for cloning
experiments

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