Unit 1 Notes
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SanjaiUnit 1 Notes
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Sanjaiubieh Re ‘Own makes role. Copues A jisely Known as Dwr aeplitation « pn Own nepllecton ws bislogical pay kn vbich, procerols un the fooronce 4 sntlation, elongation termination . 2B ypes Be ®_weplivation § b * Be a Lonainetin Don replistion + OF produas & Dvn. helices, Out « vftish one contains 4 Rentialy old for ubile. the Ofte cmtawns entively new Dvn Sumi tonsenotive Dwr replication: OB i cos 2 OM polices in uhioh egch Ns Jal ici one ve See ‘and oe y ryoplication 5 ' : Replication means the process { duplicate fr sepleation 3 Primacy Blage { -unkordtong| contra) dog : explains show the Drop rake, t% Oum Copies “Pro gh Pda soplicactien uSiy, then codes aH the Qnp so dranstaiphin & uty Rin coeles yor proreuis. by transhtim . Meseléon and leh] Spesiiment > 9 Meselson and dob Experirrent was andl experimental poo) ox Bomi conservative | Dyn ‘xtplication .° Ty 1958, Madea Meselson | * “and Franklin Stahl conducted an Sr pursirnert| “on £: calf ubich’ divides “ih 0 mines, do Study the ceplieation q Doo: Le nnn i eS —_— >: : > aeons ween abs ee ee CELLED . oar. ¥ iss re ak ‘ aa ty 8 Stiri tonteredive » Don. , . . creplication Firough Meselsm and s Bah! Experiment . i $ Bs |. gMeselson> 9% Stahl used the £. toll bactodum asa model dyslen in foi) well Known slovesh aadion ino Dyn eplicalion « + thay Started . by voniny F-Coli jn - nutritional) bith ot o medium thad tonloires te “heaury "io tope of nifiegcn (Fey IS yy. ha + Te baceswa absorbed the nitrogen usten cutivoted On a medituny conteuining hauy ISN and utilised. 54 40 creak poy ~ binlasia) moleudes., including 00 - The DwA | bacteria s “niibrogeneduus | bases was ol mouttad: yith heourty '? ny often Mon generation xf owt sin Sy - we The bactoxig wine then , drowns to ( Rnedium tortatning 4 dias” N soto Dey. fe dit rihog Hed vould hoe le for Doo : : bis cf | then, tg envy ee oa) en Mey calerterlal the 2000's density Cond 40d; : j ADBigy is "ng a tint) + ' + Py Spinning molecules Nike Drow af! hi; | Speecls while anoltur redecule duthas Cassis chloride , genencdes a clenuity guodien! from top | Jo foto. ¢ dpinning ube, this Fechniqu Aides rrrkewe Juke Dor uilo bands: ° g Minuwle changes, like Hose between By on) Var labelled PHA, tan be clotected wiry don sly gpredienh centinfucputam L Results’ o| “Bepicximnen : Clenetadion 0: > Pes. flertaain, Dwvw cohen trom ' oe ot pau of export ment (generation 0 Mig befome dusitthing 4o Mn Mediu) sferra | a dtigle bord | ; a oe , me 2 Der haut) be bead oly s hen! 8 Be and bi 7 ; ot. the finding wos veBsonall. ° ' 2 a a ae | ee the density of “Ahi beand, which udhs i higher § heheven Whe heauy a7 rel Ligh) yy DPwh, wry anleimediate + arin er “Yeon Nhe cindevrnadicne lant, Meselyon and Gohl leatneel Hat gro rrelecies treated Aung Wo tri Hal ound 4 replicon wend ag mir 4 Light % heaury pnp te clusperstive and Semi - Conemah ye models beet nod Conservative rnode/, aga with Rds esl! * ; Generation om 7 Two bands emeraed| atten eenbifugirg Seeend- generation Ove. A oe: wees higher AC seomact do be evigradeh ously wrth ty), udile Re Berend was wh the Seume place as: Unleamediade tytn, band gem the inst _genuation , ee Mita’ and Sha” ote “Unfomed. by Pas. nding +Rat om Ne was se Laewiamotives. “ : | but each Nighy Dun mole crete, oe p Rape armoke Wi mneleeetles ; 7 ha Over the 3 @ H' Gentrabions, ue: uni aotiipale thu Ugh band 40 got: Mii and = hyhid. hand qe cpt oun « a - 3 ' (en ll vom vom \P\\~ Sag a a Su Dun 92 Pete. 1p : ty IS.) My by Ws, Nowy ybid : es i i AG ne Replcaion wn ae? z . Ow: sepbeahon is an 2 ie takes ple every) Boat eah ete ee OF euLkorinhc Dyp' jection Phocers producer 2 Lenkea teptis 9 on Dun’ endleculer using fe ge tee Dep. mokeudes os: termplats « + ‘ ~ ‘ (aR a3 y ; Fi $h fine slutso hat ee The Huw suplieation sn Puokasyotes ane | “avi tnto 3 sia = Initiaton , — elongation , 7 od aa : es, ee nisin | * 2 Bunting “initotion the clifforen! _ Proletns bunols and frm tompler caulled “prisere . Pal Origin which tnuinds Hi Ova. | the diffexent tomponents and’ thelr functions “One os fellows ; «the Oxigth ; The _Heplication 4 rn snwtiabes at a Partin Sequence oe in genome , known as psel -the prokaryote cou usually Jas One Osucen as the Genome Nay Sra) “2 cieulas . _fritiod Locehing 4 replication mma chiraty Known as Oxésorre, 4) nuclear ~pscoketn cooper, and inital. anny tobe gies ot —ps a Shands Seperate mote ih Dm A m8 Pk baicls the. unwound DNA : + Ona ¢ helicase lender LThoe Proluis inteact uit SSDNA - bocen| Dm A prokesns Sona hdlicase helps the Dna .B hebicasg Ont, Re lunoeund Dn - ad e on pm hebicare = 1B tbo sels) a aah ge ene," unwinding 4 DNA: Bae | « Single - Stranded Onn binding Drolen + (298) | These prolens bind % SHabilixe te |) unwound Doe . Hene the unwound Ow Leet age et, arn, ba, pa aie Se Dwr_gyrase - Dvp Fy ase “Us 9 cepolsors thocd sernoves the dwist semtlting ann: unwinding y Dw, by eles yg % Dv hair, untuisting 94 and then *reseothing fie buckan Strode) Again . As a Mvp Ju unwound, the axe, Oppo Uke a bulble ond b Known as 9 Tephicadb'en pet a Cashel 5“ i The -vepbeation bubble has a-\Y shaped onds Known as ‘seplication forks y which us the junchior betooen 4h wounds ole DuAiand 3s unwound Dan | | i | | } | i ii ed £— Diection unuiinding The tuo unwound Shands ov | tonnplementory ond each tan dowe os the tombs or Syotiuesis { nei Shands 4 QnA * | ion , ‘ i During clengation, the va pol i) whlends he aught strands as steplivation Compley known as weplisome frowels along ‘tis length : 4 chnomosnme - | the woplisarre sincludes a Welicase, double- sone) DA, 2 wn pétymerase (8) and « bud ak tnvahad tn Prccewinn yp Primers akong laggy. Shand Sur 7 | DOD Nepris re shaniene ‘ Mest, ¢ “the ‘ 5 oe aera inelel in opine 48 DNA polymerase MH uhich ut a ding ust 9 Binalar naltiarhurit Corpleres u, 'f eompler. § pelarep m The of tomplen (ys8'x Yey loading 4 unloodery { ~ clamps , p clamps =} aing- dhaped fnolein tarpleres Rod tw 4 als aleng ee 7 albw the polymorase Sage axsoctated with Due Gon prymoiase ondymes join Be phosphale gor at 5! eatbon | now hutleotile +o- oll wou f 3 Cosbon girudleokide ob toby Because 4 tas hue ast 9 needs Pot arse? : i. Dna pol nowds On, ousting, 3 O end). | is prem sis dave by oeairg | Dra Gy. primase + Lnitiodes tre Syrers enh prim, a source f about lo Ae puuclestides Complemenieny oe DNA eroplod « - es Rep prriver provides a free on end: for adlditim g base pabrs se. The on . | a) Rephiation ‘can only Proceed in | Big ojarection - 3 Y on. polymerase th ton - nucles tides: only * ti? ered 4 Dr Shay te) - Beve o. gap Ae plethon aa prourls only in .5'—7 3) dbschon op a) yur Den Sond - Power ‘ 9: ‘pororr strates 5 Ine rule Ont pyriparallel, 3} Stand us unwound Hon S) ere} 9 other Shand From 3! end AW the Strand ubhich is Uswound tom 5! 5 pe. » Prine a3. Sunttusized, | in pol ® tan tentinuously add tutlentily bdo 9! end and exkend’ the Dior dauctles Strand. : this “Shond, udvth as Suptheinse) conbinwo uh te wm 94 lopdina Shandy. er 54
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