Vanquish Neo UHPLC System

Beyond brilliant
2
The new standard in
nano-, capillary-, and micro-flow LC
For researchers pursuing the next scientific breakthrough, the
Thermo Scientific™ Vanquish™ Neo UHPLC system offers the highest
performance, productivity, and usability of any nano-, capillary-, and
micro-flow UHPLC system.

Beyond discovery
All-in-one nano-, capillary-, and micro-flow LC for high sensitivity LC-MS workflows.

Beyond innovation
Accelerating productivity with long-term, trouble-free operation at maximum performance.

Beyond possibilities
Enabling LC-MS experts and novice users to get high-quality results, every time.

3
 All-in-one low-flow UHPLC system
Discover more in every sample with excellent system • T hermo Scientific™ ProFlow™ XR active flow control for robust
performance and unmatched retention time precision for shallow operation from nano up to 100 μL/min flow without pump
gradients, high-throughput applications, and anything in between. hardware changes
Vanquish Neo is the first UHPLC system built not only to operate,
• Factory multi-point flow calibration for precise and reproducible
but to excel across the entire low-flow range ideal for LC-MS
flow delivery of standard solvent blends
applications.
• Ultra-low 0.5 μL gradient delay volume (GDV) maintains
efficiency and ensures rapid gradient delivery for maximum
sample throughput

Recommended flow range*: 100 nL/min–100 µL/min

Settable flow range: 1 nL/min–100 µL/min

micro
400 0.5–1.0 mm I.D.
5–15 cm length
300

300
capillary

Throughput (samples/day)
Relative sensitivity gain

nano 150–300 μm I.D.

50–75 μm I.D. 5–15 cm length
200
15–75 cm length
200

ultra-low nano 100

100 <50 I.D.

0 0
0.01 0.1 1 10 100

Flow rate (μL/min), log10 scale

The Vanquish Neo UHPLC system delivers flow rates from 1 nL/min to 100 µL/min to support a broad range of
high-sensitivity LC-MS workflows. Sensitivity gain (red trace) relative to a 2.1 mm I.D. column operated at 450 µL/min
corresponds to increased electrospray ionization efficiency at lower LC flow rates. The Vanquish Neo UHPLC system
permits maximum sample throughput for a broad range of low-flow columns and applications (colored bars).
* Within specifications for retention time precision (≤0.2 % RSD or ≤0.1 SD).

Enabling precise temperature control

Ideal for micro-flow LC-MS applications, the optional
Vanquish Neo column compartment provides stable
temperature control for linear columns, coiled
capillary columns, and trap columns.

4
 Versatile high-sensitivity LC-MS workflows
From maximizing peptide identifications in discovery proteomics • Industry-leading flow-pressure footprint enables high-resolution
to high-throughput precision medicine, the Vanquish Neo UHPLC separations on ultra-long columns, high-throughput analysis on
system was designed with the versatility to tackle all your high- 1.0 mm I.D. columns and ultra-high sensitivity analysis on
sensitivity analyses. 75 μm I.D. columns without changes to pump hardware

• Optimized configurations including direct and trap-and-elute

injections improve productivity and ensure superior performance
within a broad range of LC-MS workflows

Total cycle 75 µm I.D. column Flow rate FWHM,

time (min) length (cm) (nL/min)# (sec*)
8‡ 15 1300 1.8
8‡ min
14.4‡ 15 1000 3.0
24 ‡
15 800 4.8
14.4‡ min 48 ‡
15 400 9.0
80 §
50 300 6.6
120§ 50 300 9.6
24‡ min
165 §
75 250 11
225 §
75 250 14
48‡ min 285 §
75 250 18
*  edian of 15 Pierce Peptide Retention Time Calibration Mixture (PRTC)
M ™
Relative intensity

peptides spiked into HeLa protein digest

‡ Trap-and-elute injection
80§ min § Direct injection
# Flow rate during the separation stage

120§ min

165§ min

225§ min

285§ min

50 100 150 200 250

Time (min)

The Vanquish Neo UHPLC system supports a broad range of flow rates and gradient lengths without any changes to pump hardware. For
all runs, 1 μg of HeLa protein digest was separated on a Thermo Scientific™ EASY-Spray™ PepMap™ Neo column by a Vanquish Neo UHPLC
system interfaced to a Thermo Scientific™ Orbitrap Exploris™ 480 mass spectrometer operated in data dependent acquisition mode.

5
Accelerate productivity
In your lab, system performance and productivity shouldn’t • High pressure capabilities and an ultra-low GDV ensure
be at odds. The Vanquish Neo UHPLC system enables you to maximum sample throughput for a broad range of column
consistently generate high quality data while maximizing diameters and lengths
MS utilization.
• Fast sample loading and column equilibration reduce overhead
time and improve MS utilization

Column washing

Separation gradient
Traditional method

Sample loading Equilibration

%B

Flow

Column washing

Separation gradient
Accelerated
Accelerated column
sample loading equilibration Method using
%B fast loading
and equilibration
Increased flow rate up Increased flow rate up
to maximum pressure to maximum pressure

Flow

Time
Productivity increase

Fast sample loading and equilibration increase system productivity.

500
81%
Standard
400 Fast loading
and equilibration
Samples/week

300

200
30%
18%
100

0
75 µm × 75 cm 75 µm × 50 cm 75 µm × 15 cm
Column dimensions

Comparison of sample throughput with and without fast sample loading and column equilibration.
Estimated increase in sample throughput ranges from 18–81%, depending on column dimensions.

6
 Maximize performance
Built from the ground up in order to redefine the low-flow 30
R2 = 0.9994
chromatographic system, the innovative Vanquish Neo Split
24
Sampler NT provides superior sample injection performance.

Peak Area (mAU/min)

• Unique low-flow split-loop autosampler design limits sample 18

dispersion, system carryover, and sample usage to maximize

sensitivity and data quality 12

• Novel valve and fluidic designs ensure low gradient delay 6

volume for increased system productivity
0
• Metering device delivers wide injection volume linearity, high 0 0.2 0.4 0.6 0.8 1.0
injection volume precision and accuracy, and multi-draw Injection volume (µL)

capabilities for enhanced quantitation Injection volume linearity of a caffeine standard from
0.1 to 1.0 μL using flow injection analysis.
• Multi-wash injection routines minimize system carryover for
improved sensitivity of low-level analytes

• V ial bottom detection technology enables precise and

accurate injection from limited sample volumes, decreasing
sample waste

Vial bottom detection technology enhances injection

volume linearity and precision while minimizing waste
of limited samples.

1.5 HeLa + PRTC, 200 ng

Blank

1.0
Peak area (×1010 )

0.5
0.001%

0.007%

0.070%

0.001%

0.010%

0.033%

0.016%

0.030%

0.019%

0.037%

0.036%

0.164%

0.090%

0.095%
0.031%

0.0
PR

TR

AR

LR

R
K

EK

IK

DK

TK

FK

K
SI

YS
LS

FP

L
AG

G
EE
LQ

YG
G

FD
PK
P

G
AQ
NA

PE

G
V
PP

V
SI

EE
DY

IL
TY

EV

FQ
FP
PV

LD
AI
Q

LT

LT
AP

G
G
IG

S
G

VD

PL

LS

AL
DI

AF

FI
HV

G
LV
FD
NE
A

NG
VS
Q

G
SG

AP
AG
SS

LI
SE

AN

AS
IS

SG
G

SE
Q
SA
G

TA

EL
SF
G

LS
EL

FV
IL
G

Carryover for 15 PRTC peptide standards spiked into HeLa protein digest. 200 ng was injected onto a 75 µm I.D. × 75 cm column followed
by a blank injection using a Vanquish Neo UHPLC system coupled to an Orbitrap Exploris 480 operated in data dependent acquisition
mode. Percent carryover is listed above the peak area for each blank injection. The flow rate was 250 nL/min during the separation phase.
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Long-term, trouble-free LC-MS analysis
Modern proteomics, lipidomics, metabolomics, translational • Improved column lifetime and retention time precision with
research, and bioanalysis depend on LC-MS instrumentation SmartInject technology
capable of identifying and quantifying subtle analyte concentration
• Reduced cost per sample through decreased downtime, less
differences across large sample cohorts. The Vanquish Neo
maintenance, lower solvent consumption and waste generation
UHPLC system instills confidence in your results by delivering
efficient separations 24/7 without compromising robustness. • T hermo Scientific™ Viper™ and Thermo Scientific™ nanoViper™
fittings ensure near-zero dead volume, leak-free connections

20
A

15

10
Intensity (mAU)

-5
0 10 20 30 40 50 60 70 80 90
Time (min)

Day 44 Day 88 Day 132 Day 176

B 90

80

70
Retention time (min)

60

50

40

30

20

10

0
0 400 800 1200 1600

Injection number

8 peptides were selected from a 90 minute gradient separation of 1 pmol BSA protein digest
performed at 300 nL/min with the system configured in direct injection mode (A). Retention
time stability was monitored for the selected peptides over a period of 176 days. In total,
1600 injections were performed using 1500 bar system pressure for sample loading and
column equilibration.

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Confidence in your results
Every Vanquish Neo UHPLC system is designed and rigorously • Factory pre-assembled, configured, calibrated, and tested
tested to guarantee high quality separations and system-to- during the production process for fast installation, enabling you
system reproducibility, both within labs and around the globe. to perform high quality separations from day 1 on all Vanquish
Standardized configurations improve your user experience Neo UHPLC systems
throughout the instrument’s lifetime.
• Slide-in pump and autosampler modules simplify maintenance
while the built-in tool and consumable drawer enhances
convenience

• Capillary, solvent, and waste line guides for trouble-free

connections

Protein groups Peptide groups Peptide spectral matches

80000
66K 64K 63K 70K 69K 70K
70000

60000
Number of identifications

50000

40000
33K 31K 32K 35K 34K 32K

30000

20000

10000 4474 4304 4298 4570 4435 4363

0
System 1 System 2 System 3 System 4 System 5 System 6

Results of 200 ng HeLa protein digest profiling with 6 different Vanquish Neo UHPLC systems
coupled to Thermo Scientific™ Orbitrap Exploris™ 240 systems. 75 µm × 50 cm, 2 µm
EASY-Spray PepMap Neo column, 90 min gradient, 300 nL/min flow rate, direct injection,
1500 bar sample loading and column equilibration were used.

Viper and nanoViper fittings

The Vanquish Neo UHPLC system is equipped with Viper and nanoViper fittings for leak-free operation up to 1500 bar, preventing
time-consuming troubleshooting of system fluidics. Viper capillaries limit extra column dispersion by utilizing a PEEK tip seal and
bushing instead of a traditional ferrule for improved separation performance. All 1500 bar nanoViper fittings are biocompatible and offer
high capillary-to-capillary reproducibility.

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Superior usability
The Vanquish Neo UHPLC system delivers uncompromised
results to expert LC-MS users while expanding the toolbox for
novice users for nano-, capillary-, and micro-flow LC-MS
applications. An intuitive user interface and intelligent system
control help to make the Vanquish Neo UHPLC system the most
user-friendly low-flow UHPLC system ever built. The Thermo
Scientific™ Vanquish™ User Interface provides:

• Easy system status monitoring through direct visualization of

key system parameters

• Direct instrument control without requiring a PC during system

preparation, maintenance, and diagnostics

• Automated system procedures, which can be triggered

remotely, include start-up, self-checks, and leak tests for daily
operational convenience

• Streamlined instrument troubleshooting with built-in diagnostic

procedures and guided tutorials for increased uptime and
reduced costs per injection

Guided maintenance procedures minimize instrument downtime.

Walk-up and remote system status monitoring.

Built-in diagnostic procedures reduces

time-consuming troubleshooting steps.

10
Intelligent operation
The Vanquish Neo UHPLC system features a new level of system
intelligence with inter-module communication. Automated injection
routines with optimized parameters for sample loading and
fluidics washing deliver high chromatographic performance and
reproducibility. The instrument method editor wizard ensures
column compatibility with separation parameters.

The system interlink and single

system driver provide an optimal user
experience through intelligent, direct
communication between modules,
harmonizing all aspects of the
chromatographic method execution.

Enhanced ease of use, method

robustness, and standardization
through active integration of
consumable information such as
column dimensions, pressure, and
flow limits to ensure methods adhere
to consumable specifications.

Factory pre-calibrated for

common LC-MS mobile phases,
plus guided calibration procedures
for additional solvents.

Simplified gradient programming

guided by time and flow permits the
generation of standardized methods
based on gradient or column volume.

Reduced user errors with active

notifications which appear when
operating out of the recommended
range for method parameters such
as flow rate and column washing/
equilibration times.

11
 1

7
6
8

10

11
12

12
Innovating for you
1 Vanquish User Interface 7 Vial bottom detection technology
Provides smart system operation through system status Injection from limited sample volumes with near zero sample
monitoring, automatic start-up procedures, and direct loss (up to 2.5 out of 3 μL).
instrument control without requiring a PC connection for
8 Standardized fluidic framework
high operational convenience.
Nano-, capillary-, and micro-flow LC at up to 1500 bar.
2 Ease-of-use Leak-free nanoViper fittings minimize extra column dispersion
Straightforward fluidic design with labeled lines and and GDV, maximizing throughput and separation quality.
pre-defined solvent bottle positions in the solvent rack.
9 ProFlow XR technology
3 SmartInject Technology Active flow control across the nano-, capillary-, and
Reduces pressure drop for both direct and trap-and- micro-flow ranges and factory multipoint flow calibration
elute injection, yielding high retention time precision and deliver industry-leading gradient accuracy, gradient precision,
extending column lifetime. and system-to-system reproducibility.

4 High-precision metering device 10 Fast sample loading and column equilibration

Accurate injection volumes from 10 nL to 100 μL. Sample Accelerated sample loading and column equilibration with
multiple-draw functionality extends the injection range up increased flow rate and pressure, reducing analysis time.
to 500 μL for sample analysis using the trap-and-elute
11 System interlink
workflow.
Direct inter-module communication provides superior user
5 Workflow versatility experience and system intelligence.
Choose between direct injection and trap-and-elute
12 Keep your lab tidy
workflows. For trap-and-elute, switch between forward-flush
A practical drawer allows the storage of consumables and
or backward-flush modes without replumbing fluidics.
tools for user convenience.
6 Multi-wash injection routines
Negligible system carryover through inner and outer
injection needle and fluidics wash routines automatically
executed in parallel to the sample analysis.

Optional column compartment

Temperature-controlled direct injection or heated trap-and-elute
injections provide increased system versatility.

Intelligent operation
Smart guided method creation linked to consumable and fluidic
parameters ensures robustness through optimal operation, fast
sample loading, and sufficient column washing and equilibration.

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Brilliant separations
We offer robust and reproducible nano-, capillary-, and micro-
flow columns for ultimate sensitivity and resolution. Built Linear nano- and capillary-flow columns
alongside the 1500 bar capable Vanquish Neo UHPLC system,
Standalone columns for
Thermo Scientific™ PepMap™ Neo columns enable simple,
nano- and capillary-flow are
tool-free set up and configuration. Thermo Scientific columns
designed with single nanoViper
range from 75 µm to 1 mm I.D. for improved separation
and double nanoViper trouble-
performance across the entire Vanquish Neo UHPLC system
free connectors for robust
low-flow range; ideal for LC-MS applications.
separation. Compatible with any
MS system source design, they
deliver excellent resolution, long
column lifetime, and low carry-over.
EASY-Spray columns
Thermo Scientific™ EASY-Spray™ PepMap Neo columns
ensure robust nano- and capillary-flow LC-MS analysis. The
EASY-Spray integrated column and emitter design virtually Micro-flow columns
eliminates dead volume and is temperature-controlled for
maximum reliability and performance. For outstanding peak shapes in micro-flow chromatography,
1 mm I.D. columns are available in a range of chemistries

12
PepMap Neo C18 column Emitters
PepMap C18 column

10 Nano and capillary emitters act as a

HETP (µm)

8 column-independent sprayer, allowing

PepMap Neo C18 column
12 PepMap C18 column the introduction of flow from nano
6
10 and capillary columns without the
4
HETP (µm)

80
troublesome handling of traditional
0.5 1 1.5 2
Linear velocity (mm/s) emitters and connectors.
6
PepMap4 Neo C18 columns (teal) outperform Thermo Scientific™
PepMap0 C18 (orange)
™
0.5
columns
1
of
1.5
the same
2
format—50 cm × 75 µm
I.D., 2 µm particle diameter
Linear velocity (mm/s)

Trap columns
Trap columns help accelerate sample loading and permit
on-line sample concentration and desalting as well
as ensuring protection of our entire low-flow column
portfolio. The Thermo Scientific™ PepMap™ Neo trap is
1500 bar​pressure compatible.

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Seamless MS hyphenation
The Vanquish Neo UHPLC system is designed for a wide range of • E xceptional speed, robustness, and sensitivity with Thermo
high-sensitivity LC-MS applications and flawless integration with Scientific™ TSQ™ triple quadrupole mass spectrometers
industry-leading Thermo Scientific mass spectrometry portfolio
• A broad range of electrospray ionization sources allow optimal
and ESI sources.
MS integration for your separation
• Unsurpassed resolution, mass accuracy, and versatility with
• System control with Thermo Scientific Standard Instrument
Thermo Scientific™ Orbitrap Exploris™ series and Orbitrap
Integration (SII) for Xcalibur™ or Thermo Scientific™
Tribrid™ series mass spectrometers
Chromeleon™ CDS software

Thermo Scientific™
EASY-Spray™ SII for
source Xcalibur

Chromeleon
Thermo Scientific™ CDS
Thermo Scientific
™
Nanospray Flex™
Orbitrap Exploris™ ion source
mass spectrometers

Thermo Scientific™
OptaMax™ NG
ion source

Thermo Scientific™
TSQ™ triple quadrupole
mass spectrometers

Thermo Scientific™
Orbitrap Tribrid™
mass spectrometers

15
Thermo Scientific LC portfolio overview
Analytical HPLC and UHPLC Nano-, cap-, micro-flow
UHPLC
Application-specific HPLC and UHPLC
Thermo Scientific™ Vanquish™ Duo LC Systems
Thermo Scientific™ Vanquish™ Method Development LC Systems
Thermo Scientific™ Vanquish™ Online 2D-LC Systems
Thermo Scientific™ Transcend™ LX and TLX LC Systems

Dependability Flexibility Performance Sensitivity

Thermo Scientific™ Thermo Scientific™ Thermo Scientific™ Thermo Scientific

Vanquish™ Core HPLC Vanquish™ Flex UHPLC Vanquish™ Horizon UHPLC Vanquish Neo UHPLC
Systems Systems System System

• High-pressure binary, • High pressure binary and • High-pressure binary solvent • All-in-one nano-, capillary-,
low-pressure quaternary low-pressure quaternary blending and micro-flow LC systems
solvent blending or isocratic solvent blending • Biocompatible • Accelerating productivity with
flow delivery • Biocompatible long-term, trouble-free
• Industry-leading specifications
• Workhorse instrument • Unparalleled flexibility operation
• Unmatched detection
• Seamless method transfer and control sensitivity • Intelligent at-system or remote
• System intelligence for control, method setup,
improved productivity diagnostics, and troubleshooting

700 bar 1000 bar 1500 bar 1500 bar

Learn more at thermofisher.com/vanquishneo

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products in any manners that might infringe the intellectual property rights of others. BR74142-EN 0721M