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Leica M205 FCA & Leica M205 FA

Fluorescence stereo microscopes
Capture bright images – fast!

Do you sometimes feel like you are fishing in the

dark because you are looking for fluorescence
signals that are increasingly faint and weak?
Brightness and high resolution images are a must
in modern developmental biology and research.

Leica Microsystems has developed the M205 FA

and M205 FCA fluorescence stereo microscopes
to enable you to detect transgenic expression like
GFP and mCherry in early stages, allowing you to
select the right sample to successfully base your
studies on.

Leica M205 FCA: semi-automated with

manual zoom
Until now, you probably had to switch between
two different systems: one for fast screening with
a manual zoom that is intuitive to maneuver and a
high-end solution to see and capture the faintest
signals in an image.

The Leica M205 FCA fluorescence stereo

microscope unites both worlds and combines
super-fast manual screening with high-end
imaging capabilities.

Now you can have it all:

Get highly reliable and publishable results as

all parameters are always auto-stored for you

Eliminate interruption in your workflow with

the four position coded filter changer

Add a footswitch for easy change of filters,

focus, and illumination adjustment, keeping
both hands free for screening

Video of c.elegans pharynx with mCherry. Courtesy

of Martin Gamerdinger, Ph.D., Scientific Project
Leader, Molecular Microbiology, University of
Constance, Germany

Discover the automated world of

research
Leica M205 FA fully motorized fluorescence
stereo microscope

The Leica M205 FA opens up a new world of

research in fluorescence microscopy, for instance
when working in a sterile cabinet.

Easily handle complex multi-channel

fluorescence imaging procedures

Execute demanding experiments with the

motorized zoom, filter changer, Fluroescence
Intensity Manager (FIM), and iris diaphragm

Get high resolution at the stage level: the Leica

LMT 260 scanning stage positions your sample
in the sub-um range and holds life cell
incubation equipment

Bright fluorescence signals – always.

To detect even the faintest signals of your sample,
you need an energy-rich excitation light to give a
bright fluorochrome signal.

However, the excitation light may cause

reflections that blur the black background and
compromise the detection of fluorescence signals.

Leica’s TripleBeam technology completely

eliminates this background “noise” by introducing
a third optical zoom. This separates the
fluorescence excitation light from the two
observation channels and omits the need for a
dichroic mirror.

The result is a clear and strong fluorescence

signal against a noise-free, black background.

Finest details in 3D
Do you think that high resolution and maximum
depth of field are irreconcilable opposites in
microscopy? We prove they are not! Leica
Microsystems' FusionOptics technology
overcomes optical limitations by using the two
beam paths for different tasks:

The right channel delivers a high-resolution

image at the largest possible numerical
aperture.

The left channel presents an image with high

depth of field.

The result: you perceive an image with

outstanding richness of detail and exceptional
depth of field at the same time

Microscopic resolution in a stereo

Recognizing fine details is vital in research
especially when working with small organisms.
The Leica M205 FA and M205 FCA can achieve a
resolution of 1279 lp/mm or 0.78 µm based on the
resolution limit defined in ISO18221*.

The 2.0x PlanApo objective is an optical

masterpiece. It has a maximum aperture of 0.35 –
that’s the highest numerical aperture (NA) that has
ever been achieved with a stereo microscope. This
is smaller than a tenth of the diameter of a human
red blood cell.

*M205 FA, 2x PlanAPO objective, camera adapter

0.63x, DMC4500 camera in full frame mode, Apple
5K with 27” monitor.

Neuronal cell culture stained with DAPI, beta III

Tubulin–Cy2, Nestin-Cy3 (LMS Bioanalytik GmbH,
Magdeburg, Germany). Blue indicates the nuclei of
the cells, green neurons expressing beta III Tubulin,
and red stem cells expressing Nestin. Image was
acquired with a M205 FCA stereo microscope,
LMT260 x/y stage, DFC3000 G microscope camera
and Fluocombi III at 400x.

Speed matters – encoding helps

Image Coding provides convenient, reproducible
settings for quick, easy documentation.

The integrated encoding transmits both the

magnification and the position of the iris
diaphragm to the software in real time. A scale
bar is overlayed into the live image and updated
when the magnification is changed. When an
image is stored, all the settings are saved with the
image and can be recalled at any time.

Coded components offer ease of use and

reliable results even for untrained operators

The microscope system is intelligently linked

to the software to allow you to change settings
without having to manually adjust the
calibration

TL5000 Ergo transmitted light base adapts the

aperture automatically to the zoom position to
deliver optimal contrast

Zebrafish, mpx:eGFP line: time lapse images of the

neutrophils to the tail resection. Courtesy of Dr Carl
Tucker, The Queen’s Medical Research Institute,
University Edinburgh, United Kingdom. In this in
vivo model the inflammatory response in a
transgenic zebrafish line that expresses GFP under
the neutrophil-specific myeloperoxidase promoter is
shown. “Quantitative data can be generated from
this model by counting of fluorescent cells or by
digital image analysis” (Renshaw et al Blood 2006)
DOI 10.1182/blood-2006-05-024075

2 x CORR objective – making it clear!

With the Leica PLAN APO 2.0x CORR Objective,
the refractive index can be adjusted – this way
you will have pin-sharp images even if there is a
water column of 5 mm between your sample and
the objective. The objective enables users to
observe and document samples as if the water did
not exist.

When observing samples immersed in a watery

solution, structures tend to blur especially in high
magnification. This is due to the refractive index
mismatch of air (index = 1) and water (index = 1.3).
Potentially interesting or important structures may
be easily misinterpreted because of spherical
aberrations that may occur, as regular objectives
are dedicated to the use with samples surrounded
by air only.

Fluorescence video imaging of a zebrafish larva

expressing green fluorescent protein (GFP)
immersed in an aqueous solution. The images were
captured with a Leica M165 FC stereo microscope
where a Leica 2x Plan Apo objective with (right
image) and without (left image) correction collar was
used. The heart of the zebrafish larva is seen beating
in both videos. The larva appears blurrier in the non-
corrected image on the right. Courtesy of M. J.
Hamm and W. Herzog, Angiogenesis Laboratory,
Max Planck Institute for Molecular Biomedicine and
Westfälische Wilhelms University in Münster,
Germany.

Explore LAS X Software for Life

Sciences
LAS X is the software platform for all Leica
microscope solutions. Run complex fluorescent
experiments with ease. LAS X guides you step-by-
step through the entire analysis workflow.

Live data mode: design experiment patterns

and control your environment

Extended depth of field (ED(O)F)

Create unparalleled large overview images

combining deconvolved z-stacks with XY

Solea senegalensis larvae nervous system, max

projection of a tile scan of 6 fields x 33 planes.
Parallax correction and tiling performed in LAS X
after deconvolution with Huygens professional.
Courtesy of Dr. Marco A. Campinho, CCMAR - Centre
for Marine Sciences, Universidade do Algarve,
Portugal.

Interested to know more?

Talk to our experts. We are happy to
answer all your questions and concerns.

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