Lab # 10

Date: October 15, 2021

Title: Food Test: Identifying Nutrients in Food.
Aim: To find out the types of nutrients present in various food substances.
Apparatus and Materials:
● Test tubes ● Bunsen burner ● Benedict’s solution ● Alcohol
● Beakers ● Tripod stand ● Iodine solution ● Distilled Water
● Test tube rack ● Dropper ● Dilute hydrochloric acid ● Food Samples
● Test tube Holder ● White Tile ● Sodium hydrogen carbonate
● Dilute Sodium hydroxide Solution ● 1% Copper Sulphate Solution
Method/Procedure:
TABLE SHOWING THE RESULTS OF THE FOOD TESTS FOR VARIOUS FOOD
SUBSTANCES.
METHOD OBSERVATION INFERENCE
(State at the start, during and
after the test)
TEST FOR REDUCING When Benedict's solution was Test Tube A tested positive for
SUGAR added to the food solution it Reducing sugar while Test
2cm of the food solution was
3
became blue while boiling it tubes B,C and D tested
placed in a test tube, 2cm of
3
changed to a Red-brown color negative for reducing sugar as
Benedict’s solution was added and and when the test was over the they did not show the same
the mixture was boiled for 2 solution became Red. This test color change as Test Tube A.
minutes. tube was labeled A.
TEST FOR NON-REDUCING At the beginning the Test tube B tested positive for
SUGAR substance was clear (white) Non Reducing sugar while
A fresh tube of 2cm food solution
3
after adding Benedict’s Test tubes A,C and D tested
with dilute hydrochloric acid was Solution it changed to a blue negative for Non reducing
boiled for 2 minutes in a hot water color while boiling the sugar as they did not show the
bath and removed. Sodium substance changed to a green same color change as Test
hydrogen carbonate was added to color after boiling it changed to Tube B.
the content of the tube until the a orange color. This test tube
contents stopped fizzing. 2cm of3
was labeled B.
Benedict’s solution was boiled for
1 minute.
TEST FOR STARCH At the beginning the food The sample tested positive for
2cm of the food sample was placed solution was a light yellow
3
Starch while the other samples
on a white tile. Five drops of iodine color when the Iodine was tested negative.
solution were added to the sample. added it slowly became darker
until the spots that were
exposed to iodine became a
Blue-black color.
TEST FOR PROTEINS At the beginning of the Test Tube C tested positive for
 2cm of food solution was placed
3
experiment the solution was Proteins.
into a test tube and 5cm of 1%
3
white it then turned blue when
Copper (II) Sulphate Solution the Copper Sulphate was added
(CuSO ) was added to it. The test
4 and after the Potassium
tube was then gently agitated then Hydroxide was added the
5cm of 5% Potassium Hydroxide
3
solution turned purple. This test
(KOH) was added. tube was labeled D.
TEST FOR FAT The distilled water had cloudy Test Tube D tested positive for
(Emulsion Test) emulsion above it inside the Fat while Test Tubes A, B and
2cm of food solution was placed
3
tube. A milky white emulsion C tested negative for fat as
into a test tube. 3cm of alcohol
3
formed below the mixture. This they did not show the same
was added to it and was thoroughly test tube was labeled D. color change as Test Tube D.
shaken for 30 seconds. 10cm of 3

distilled water was poured in a

separate tube. The food and alcohol
mixture was poured in a test tube
with distilled water.
Grease Spot Test At the beginning there was no The filter paper tested positive
Food sample was rubbed on a filter change but after both samples for fat.
paper and a drop of water was dried the filter paper with the
placed on another filter paper. food sample showed a grease
They both were dried and spot while the paper with the
compared. water showed no change.