as Telangana State Council of High B.Sc, CBCS Common Core Syllabi for ion, Govt. of Telangana ies in Telangana (w.e.f. 2019-20) PROPOSED SCHEME FOR CHOICE BASED CREDIT SYSTEM IN BSc., BIOCHEMISTRY I Course Type | Course Title | Credits I Cour eTitle | “TAECC | vironmental Science 2? LIA [English 14 [4 LA Second Language 4 4 DSC-1A | Chemistry of Biomolecules DSC-2A | Optional II DSC-3A | Optional IIT — | Toran iT AECC 2 Basic Computer Skills [2 LIB English L268 Second Language DSC -IB ‘Chemistry of Nucleic acids and Biochemical Optional 11 | Optional Ii [TOTAL Basics in Biochemical | 2 2 calculations and Biostatistics English Second Language | 3 Bioenerge s Biological oxidation and Enzymok Optional IL Optional III TOTAL Applied and | ‘Computational Biochemistry - | CH. A Aoard of Studies In Biochemist DERABAD-S10 6[Bs 402 BS 403 al as BS 404 3 3 BS 405 4T+2P=6 | 441-5 BS 406 [Optional 11 [BS 407 | Optional 11 - | SEMESTER-V - - | BS 501 GE aT 4 __| Biochemistry 2 BS 502 [L. English _| BSS03_|L-2E | Second Language _ DSE-AE ‘A - Physiology, BS 504 Nutrition and CI Biochemistry B- Cell Biology, | Genetics and | {_ __| Microbiology BS 505, DSE-2E Optional 11 A/B - | BS 506 DSE-3E Optional 111 A/B “TTOTAL | SEMESTER-VI - BS 601 | L-IF English BS 602 LF ‘Second Language DSE-IF ‘A- Molecular Biology BS 603 and Immunology B-r-DNA technology seseeeseaaa and Biotechnology BS 604 Optional I A/B 4T+2P=6 | BS 605 D ~__| Optional 1 A/B 4T+2P=6 BS 606 Optionals (Theory) 4 Biochemistry in Health - and Disease | | TOTAL 3B CE _ TOTAL CREDITS 150 | AECC- Ability Enhancement Compulsory Course DSC- Discipline Specific Core SEC- Skill Enhancement Course DSE- Discipline Specific Elective GE- Generic Elective HPW — Hours per week *Credits under Non-CGPA : i. NSS/NCC/Sports/Extra-curricular ~ 2 in eachy€ar (up to 6) ii, Summer internship ~2 in each after I & J Cw Adu CHAIRMAN ard of Studies in Biochamis! ‘Osmania University HYDERABAD-600 067Semester - 1 DSC-1A Semester ~ I: Paper-BS104 (Theory): Chemistry of Biomolecules (4 Credits: 4 Hriweek) Credit- I: Introduction Scope of Biochemistry Water as biological solvent Weak acids and bases 4. pH and concept of Butters 5. Biological buffers and their physiological importance 6. Henderson- Hasselbalch equation (Simple numerical problems) erence to Carbohydrates 3 Concept of Stereo chemistry with =H: Am jo acids & prot |.Classification, structure, stereochemistry and chemical reactions of amino acids. 2. Titration curve of glycine & pk values. 5 Essential, nonessential amino acids and non-protein amino acids. 4.Peptide bond- Nature and conformation, Naturally occurring peptides ~Glutathione and Brain peptides (Enkephalin) 5.Outlines of protein classification, structural organization of proteins: primary and quatemary structures (ex. hemoglobin & myoglobin) 6.General properties of proteins, denaturation and renaturation of proteins. 7.Determination of amino acid composition of proteins. secondary, teria Credit IIL: Carbohydrates 1 Classification of carbohydrates 2. Monosaccharides : Structures, Fisher and Haworth projections 3.Reactions of monosaccharides, Mutarotati 4.Amino sugars and Glycosides 5.Disaccharides, Oligosaccharides and Polysaccharides 6.Storage and Structural Polysaccharides 7.Glycosaminoglycans and Bacterial cell wall polysaccharides. Credit ~ IV: Lipids 1 Classification of lipids, Reactions & properties of lipids 2.Saturated, Unsaturated and Essential fatty acids ructure and functions of Neutral fats, waxes, phospholipids, sphingolipids, 4.Sinicture and functions of cholesterol and glycolipids / 5 Prostaglandins and lipoproteins. e wy Aw Qe 36.Bio membranes, behavior of amphipathic lipids in water, formation of micelles, bilayers. vesicles Liposomes. 7.Membrane composition and fluid mosaic model. References: Lehninger’s Principles of Biochemistry ~ Nelson.D.L.. and Cox.M.M., Freeman & Co. Biochemistry ~ Berg.J.M., Tymoczko.J.L. and Stryer.L., Freeman & Co, Biochemistry ~ Voet.D and Voet.,1.G., John Wiley & Sons Textbook of Biochemistry ~ West E.S.,Todd, W.R,Mason.H.S..and, Bruggen, J.T.V.. Oxford & 1BH Publishers Outlines of Biochemistry ~ Conn-£.£.,Stumpf.P.K., Bruening, Gand Doi.R.H., John Wiley & Sons Harper's Illustrated Biochemistry — Murray, R.K., Granner.D.K. & Rodwell,V.W., McGraw-Hill Bichemistry-Lippincott's Illustrated Reviews. Champe, P.C. and Harvey, R. A. Lippincott Fundamentals of Biochemistry Jain, J.L., Jain,S., Iain, N. S. Chand & Co. Biochemistry ~ Satyanarayana. U and Chakrapani. U, Books & Allied Pvt, Lt,DSC -1A ‘Semester ~ I: BS 104; Practicals: Qualitative Analysis of Biomolecules (1 Credits; 2 Hr/week) Laboratory general safety procedures Preparation of standard solutions ( Molar, Normal and percent solutions) Determination of pKa values of amino acids by titration (Glycine) Preparation of buffers (Acetate and Phosphate buffers) Qualitative identification of Carbohydrates Qualitative identification of Amino acids Qualitative identification of Lipids References |. Experimental Biochemistry-A student companion-Beedu Sashidhar Rao and Vijay Deshpande. 2. Laboratory Manual in Biochemistry- Jayaraman, J. Wiley EasternSemester - I DSC -1B Semester ~ I: Paper-BS204 (Theory) Chemistry of Nucleic Acids and Biochemical Techniques (4 Credits; 4 Hr/week) Credit - 1: Composi Mn of Nucteie acids Nature (functions) of nucleic acids. Structure of purines and pyrimidines. Nucleosides and Nucleotides DNA & RNA Stability and formation of phosphodiester linkages Effect of acids, alkali and nucleases and phosphodiester linkages Photochemical and Spectral characteristics of Nucleic acid U: Structure of nucleic acids Watson& Crick DNA double helix structure Introduction to circular DNA, supercoiling, helix to random coil transition, denaturation of nucleic acids. Hyperchromic effect Tm values and their significance. Reassociation kinetics, cot curves and their significance, Different types of RNA and their biological funetians 1 4 6 7. Credit - I1I: Spectrophotometric and Centrifugation Techniques 1 2 4 5. 6 (Credit ~ 1V: Chromatography and Electrophoresis techniques 1 4 5. 6 7 Colorimetry and spectrophotometry Beer-Lamberts law and its limitations, UV and Visible spectra Molar extinction coeffici Principle of fluorimetry Principle of Centrifugation techniques Types of centrifugation and their applications Introduction and principles of chromatographic techniques Paper chromatography and applications Thin layer chromatography and applications Gel filtration (molecular sieve) chromatography Jon exchange Chromatography Affinity chromatography Principle of electrophoresis and applications: Native, SDS-PAGE and Agarose electrophoresis Ohu nouReferences 1 Biochemistry ~ Voet.D and Voet, G., John Wiley & Sons Textbook of Biochemistry ~ West E:S..Todd.W.R,Mason.H.S.and, Bruggen, J.T.V., Oxford & IBH Publishers, Quiles of Biochemistry ~ Conn.E.E.Stumpf.P.K., Bruening. G and Doi RH., John Wiley & ‘Sons principles and Techniques of Practical Biochemistry Wilson, K. and Walker, J. Cambridge Press, The Tools of Biochemistry- Cooper, T.G, John Wiley & Sons Press Physical Biochemistry- Friefelder, D. W.H. Freeman Press. Analytical Biochemistry ~ Holme-D.J. and PeckH., Longman, Piophysieal Chemistry: Principle and techniques: Upadhyay A, Upadhyay K and Nath. \ Himalaya Publishing House, Experimental Biochemistry- Clark Jr. J.M and Switzer, R. L. Freeman & Co.DSC ~1B Semester ~ Hl: Paper-BS204; Practicals: Quantitative Analysis of Bi olecules (1 Credits; 2 Hr/veek) ‘Amino acid Estimation by Ninhydrin method Protein Estimation by Biuret Protein estimation by Folin's Method Estimation of Total Sugars by Anthrone Method Estimation of Total Reducing Sugars by Dinitrosalicylate method Estimation of Keto sugar by Roe's resorcinol Method Estimation of total sugars by Phenol-sulphuric acid method References |. Experimental Biochemistry-A student companion-Beedu Sashidhar Rao and Vijay Deshpande. 2. Laboratory Manual in Biochemistry- Jayaraman, J. Wiley EasternCredit. Credit. SEMESTER-AIL EC ‘Semester III- Paper BS 301: BASICS IN BIOCHEMICAL CALCULATIONS AND BIOSTATISTICS (2 Credits; 2 Hriweek) Basic Biochemical Caleulations 1. Units and measurements 2. Concentration of analyte: Mole, Molarity, Normality and percent solutions 3. Concept of density and specific gravity 4. Enzyme activity, Specific activity and Purity index 5. pH scale and measurement of redox potential 6. Concept of buffers and Buffer preparations 7. Construction of calibration curve and absorption curve (Amax) Biostatistics |. Basic statistical concepts: Population, sampling and variables 2. Biostatistics: Measures of central tendency (Mean, Median Mode) 3. Measurement of dispersion: Standard deviation, standard error, Spread sheets 4. Depiction of data by graphical methods Test 6. Regression and Correlation, precision and accuracy 7. ANOVA References 4 Experimental Biochemistry-A student companion-Beedu Sashidhar Rao and Vijay Deshpande Laboratory Manual in Biochemistry- Jayaraman, J, Wiley Eastern Enzyme Assays- A practical Approach: Eisenthal, R and Dawson,M.L, IRL Press, Biostatistics ~ Arora & Malhan, Himalaya Publishing House.DSC~1C |: Paper-BS30S (Theory): BIOENERGETICS, BIOLOGICAL OXIDATIONS AND ENZYMOLOGY (4 Credits; 4Hr/week) Semester ~ 1 ioenergetics Laws of thermodynamics Energy transformations in the living syste Free energy, Enthalpy and Entropy concepts, Exergonic and endergonic reactions, High energy compounds, Substrate level phosphorylation Phosphate group transfer potential Cytochromes-structure, types and their functions Credit ~ 11: Biological Oxidations 1. Biological oxidations: Definition, enzymes involved- oxidases, dehydrogenases and oxygenases. Redox reactions. Redox couplers. Reduction potential (¢, &, e’,). Standard reduction potential (€',) of some biochemically important half reactions. Ultrastructure of mitochondria, Electron transport chain (ETC) and carriers involved, 4. Oxidative phosphorylation, theories of oxidative phosphorylation- Mitchell's chemiosmotic theory. F, Fi- ATPase, Inhibitors of ETC and oxidative phosphorylation, uncouplers, 5. Formation of reactive oxygen species and their disposal through enzymatic reactions 6 Ultrastructure and functions of chloroplast 7. Cyclie and non-eyclie photophosphorylation. Credit. 111 : Introduetion to Enzymology Introduction to biocatalysis, differences between chemical ard biological catalysis. 1 2. Principles of energy of activation, transition state 3. Nomenclature and classification of enzymes. 4. Definition of holo-enzyme, apo-enzyme, coenzyme, cofactor, Fundamentals of enzyme assy enzyme units 5. Enzyme specificity. Active site. 6. Interaction between enzyme and substrate- lock and key, induced fit models, 7. Methods of Enzyme purification IV: Enzyme Kineties and Enzyme action 1. Rate of a Reaction — Law of Mass action, Factors affecting the catalysis- substrate concentration, PH, temperature, Time, Enzyme concentration and Product eoncentration 2. Michaelis - Menten equation for single substrate reaction, significance of Kw and V.,. 3, Enzyme inhibition. irreversible and reversible, types of reversible inhibitions- competitive non-competitive, 4. Ouiline of mechanism of enzyme action- acid-base catalysis, covalent catalysis, electrostatic catalysis, and metal ion catalysis. 5. Regulation of enzyme aetivity-allosterism and co-operatitvity, ATCase as an allosteric enzyme Zymogen activation activation of tzypsinogen and chymotrypsinogen 7._ Isoenzymes (LDH) and Multienzyme complexes (PDH). Ribozyme. Uny nous idReferences: 1 2 4 10. 12, Lehninger’s Principles of Biochemistry ~ Nelson.D.L. and Cox.M.M., Freeman & Co, Biochemistry ~ Berg.J.M., Tymoezko.J.L. and Stryer.L., Freeman & Co. Biochemistry ~ Voet.D and Voet,,J.G., John Wiley & Sons Textbook of Biochemistry ~ West.E.S.,Todd.W.R,Mason.H.S.and, Bruggen, J.T.V.. Oxford & |BH Publishers. Outlines of Biochemistry ~ Conn.£.£.,Stumpf.P.K., Bruening, G and Doi.R.H., John Wiley & Sons Harper's [Ilustrated Biochemistry ~ Murray, R-K., Granner.D.K. &Rodwell,V.W., McGraw-Hill Bichemistry-Lippincott’s Illustrated Reviews. Champe, P.C. and Harvey, R.A. Lippincott Fundamentals of Biochemistry Jain, J.L., Jain, ., Jain, N. S. Chand & Co. Biochemistry ~ Satyanarayana. U and Chakrapani, U, Books & Allied Pvt. Ltd. Fundamentals of Enzymology ~ Price.N.C.andStevens.L., Oxford University Press, Understanding Enzymes — Palmer.T., Ellis Harwood, Enzymes ~ Biochemistry, Biotechnology, Clinical Chemistry ~ Palmer.T., Affiliated East-West PressDsc-1¢ Semester ~ I: Paper-BS305 (Praeticals); ENZYMOLOG (1 Credits; 2Hr/week) Assay of salivary a-amylase Assay of amylase from sweet potatoes Assay of urease 4. Assay of phosphatase 5. Determination of optimum temperature for amylase & Determination of optimum pH for amylase 7. Effect of Substrate concentration of amylase activity References |. Experimental Biochemistty-A student companion-BeeduSashidharRao and Vijay Deshpande. 2. Laboratory Manual in Biochemistry- Jayaraman, J. Wiley Eastern 3. Enzyme Assays A practical Approach: Eisenthal, R and Dawson,M.l, IRL Press. Biochemical Methods- Sadasivam,S and Manickyam,A.. New Age Intemational Publishers.SEMESTERAV SEC-3 Semester ~ III: Paper BS 401: APPLIED AND COMPUTATIONAL BIOCHEMISTRY (2 Credits; 2 Hr/week) 1: Enzyme and Protein purifieation methods. 1, Homogenization techniques 2. Centrifugation methods 3, Ammonium sulfate precipitation and Dialysis 4. Column chromatography and determination of molecular weight 5. UV-Vis spectrophotometry 6 1 Native PAGE SDS-PAGE Credit-I1: Computational Biochemistry |. Introduction to Computational Science and applications 2. Sofiware packages used in Docking studies 3. Principles of molecular modeling-Drug designing 4. Drug-Biomolecule, Receptor-Biomolecule interactions 5. Applications in Enzyme Kinetics (Km & Vax) 6. Metabolic databases (KEGG) 7. Gene Identification, Protein Lata tsank References: An Introduction to Computational Biochemistry by C. Stan Tsai, A JOHN WILEY & SONS. INC., PUBLICATION ‘Computational Biochemistry and Biophysics by Oren M. Becker, Alexander D. MacKeeell J. Benoit Roux, Masakatsu Watanabe. CRC Press, Taylor & Francis Group. Applied Biochemistry and Bioengineering by Lemuel Wingard, JR., Ephraim Katchalski-l ‘Leon Goldstein, Academic Press Inc. Protein purification ~ Principles and practice by Robert K. Scopes, Springer-verlag Protein purification ~ Principles, High resolution methods and applications by Ja Wiley Enzyme purification and related techniques, Vol 22, Nathan KaplanNathay Colowick. Elsevier hrister Janson,Dsc-1D Semester — IV: Paper-BS405 (Theory): INTERMEDIARY METABOLISM (4 Credits; 4Hriweck) Amino acid Metabolism 1. Generat reactions of amino acid metabolism- transamination, decarboxylation and deamination 2. Urea cycle and regulation 3. Catabolism of carbon skeleton of amino acids- glycogenic and ketogenic amino acids. 4. Metabolism of glycine, serine, aspartic acid, methionine, phenylalanine anc leucine. 2. Biosynthesis of creatine. Inbor errors of aromatic amino acid Inborn errors of branched chain amino acid metabolism, Credit- Il: Carbohydrate Metabolism Glycolysis, energy yield. Fate of pyruvate - formation of lactate and ethanol Citrie acid eycle, regulation, energy yield, amphipathic role. Anaplerotic reactions. Glycogenolysis and glycogenesis. Pentose phosphate pathway. Gluconeogenesis, Photosytnthesis- Light and Dark reactions, Calvin cycle and C,Pathway. CAM Pathway Metabolic disorders of carbohydrates ~ Galactosemia and Pentosuria (Credit — III: Lipid Metabolism ‘even and odd number of carbon atoms, Ketogenesis 1. Catabolism of faty acids (B- oxids 2. de novosynthesis of fatty acids 3. Elongation of fatty acids in mitochondria and microsomes Biosynthesis and degradation of triacylglycerol 5. Biosynthesis of lecithin, 6. Biosynthesis of cholesterol 7. Metabolic disorders of lipid metabolism ~ Nieman-pick disease and Fabry's disease Credit ~ LV : Nucleic acid Metabolism 1. Biosynthesis of purine and pyrimidine nucleotides, de novo and salvage pathvays, 2. Regulation of purine and pyrimidine nucleotides 3. Catabolism of purines and pyrimidines. 4. Biosynthesis of deoxyribonucleotides- ribonucleotide reductase their significance, Disorders of nucleotide metabolism- Gout, Lesch- Nyhan syndrome. Biosynthesis of heme Degradation of heme if thymidylate synthase and clu nowReferences Lehninger's Principles of Biochemistry ~ Nekon.D.L. and Cox.M.M., Freeman & Co, iochemistty~ Berg.J.M,, TymoczkoL. and StyerL., Freeman & Co Biochemistry ~ Voet.D and Voet. JG. John Wiley & Sons Textbook of Biochemistry ~ West E.S.,Todd W.R Mason H.S.and. Bruggen, J.1-V.. Oxford & IBH Publishers. Principles of Biochemistry: General Aspests-Smith, EL. Hill, R.L Lehman, 1. R Lefkowitz, RJ. Handler, P,,and White, A, MeGravt-Hill 6. Outlines of Biochemistry ~ Conn.£.£, StumptP.K., Bruening, G and Doi RH. John Wiles & Sons 7. Harper's tlustrated Biochemistry ~ Murray, 8.K., Granner.D.K. &Rodwell,V.W., MeGiraw- Hill 8. Bichemistry-Lippincot’'s Mlusrated Reviews. Champe. P.C. and Harvey. A Lippincott 8. Fundamentals of Biochemistry Jain, LL Jain, S.. Jain, N. 8. Chand & Co. 9. Biochemistry - Satyanarayana, U and Chakrapani, U, Books & Allied Pv. Ltd 10. Biochemistry — Rama Rao. A and RatnaKumari. D, Kalyani Publishers. 11, Biochemistry- The Molecular Basis of Life - McKee. T and McKee, J. R, McGraw-Hill.Dsc-1D Semester ~ IV: Paper-BS405 (Practicals): BIOCHEMICAL PREPARATIONS AND 1 References ‘SEPARATIONS (1 Credits; 2Hr/veek) Isolation of egg albumin from egg white. Isolation of cholesterol from egg yolk. Isolation of starch from potatoes, Isolation of casein from milk Separation of amino acids by Paper chromatography ‘Separation of Plant pigments by TLC Absorption maxima of colored substances- p-Nitrophenol, Methyl orange, BSA and DNA |. Experimental Biochemistry-A student companion-Beedu Sashidhar Rao and Vijay Deshpande. 2. Laboratory Manual in Diochemistiy= Jaya Wiley Eastern lo1 4 Semester -V Semester ~ V: Paper ~ BS 501: Biochemistry and Physiology (4 Credits; 4 Hr/week) iomolecules Water properties, pH and Butfers Carbohydrates — Classification (mono, di, oligo and poly), properties and importance Amino acids ~ Classification, properties and importance. Structure of proteins Lipids ~ Classification, properties and importance Nusleic acids ~ Purines, Pyrimidines, Nucleosides, Nucleotides. Structure and types of DNA and RNA and denaturation Enzymes ~ Classification, Factors affecting enzyme activity, Clinically important enzymes (SGOT, SGPT, LDH and CPK) Vitamins (Fat soluble and water soluble) and Trace elements Credit - Il: Metabolism 1 4 5 6 1 Credit - 1 4 5 6 1 Amino acid metabolism — General reactions, metabolism of aromatic amino acids Carbohydrate metabolism — Glycolysis and TCA cycle Gluconeogenesis and Glycogen metabolism Lipid metabolism - B-oxidation of fatty acids De novo synthesis of fatty acids Nucleic acid metabolism ~ Synthesis and degradation of purines and pyrimidines Metabolic disorders = IIL: Physiology Physiology of digestion Physiology of vision Physiology of muscle Physiology of nerve and mechanism of nerve impulse transmission Composition of blood and blood coagulation Structure of heart and cardiae eycle Factors controlling blood pressure ndocrinology Introduction to Endocrinology and Organization of endocrine system Hormones of Hypothalamus Hormones of Pituitary Hormones of Thyroid and CI Hormones of Panereas and C| Hormones of Adrenal gland Hormones of GonadsReferences Lehninger's Principles of Biochemistry ~ Nelson.D.L. and Cox.M.M., Freeman & Co. Biochemistry ~ Berg.J.M., Tymoczko.J.L. and Styer.L., Freeman & Co. Biochemistry ~ Voei.D and Voet, .G., John Wiley & Sons Textbook of Biochemistry ~ West S.,Todd.W.R.Mason.H1.S.and, Bruggen, J.1.V.. Oxford & IBH Publishers. 5. Principles of Biochemistry: General Aspects-Smith, EL. Hill, R.L. Lehman, |.R. Lefkowitz RJ, Handler, P., and White, A. McGraw-Hill 6. Outlines of Biochemistry ~ Conn.£.£.StumpEP.X., Bruening, Gand Doi R.H.. John Wiley & Sons Harper's Illustrated Biochemistry ~ Murray, RK , Granner.D.K. &Rodwell,V.W., MoGraw-Hill 8. Bichemistry-Lippincotts Illustrated Reviews. Champe, P.C. and Harvey. R. A. Lippincott 8. Textbook of Biochemistry and Human Biology - Talwar, G.P. and. Srivastava. L.M.. Printice Hall of India 9. Human Physiology ~ Chatteree.C.C, Medical Allied Agency 10. William's Textbook of Endocrinology — Larsen, R. P. Korenberg, H. N. Melmed, S. and Potensky. K. S. Saunders 1. Lehninger’s Principles of Biochemistry ~ Nelson.D.L. and Cox.M.M., Freeman & Co. 12, Biochemistry - Berg.J.M., Tymoczko.J.L. and Stryer.L., Freeman & Co 3. Fundamentals of Biochemistry —Jain, .L., Jain, S, Iain, N. S. Chand & Co,DSC-1E S04 A (Theory) : Physiology, Nutrition and Cli (4 Credits: AHr/veek) mistry |. Digestion and absorption of carbohydrates, lipids and proteins. 2. Composition of blood and coagulation of blood 3. Hemoglobin and transport of gases in blood (oxygen and CO.) 4. Heart structure of the heart, Cardiac cycle, cardiae factors controlling blood pressure 5. Physiology of Vision 6. Muscle: kinds of muscles, structure of myofibr ‘mechanism of muscle contraction, 7. Structure of Neuron and propagation of nerve impulse |. organization of contractile proteins and Credit-Il: Endocrinology | Endocrinology- organization of endocrine system, Classification of hormones, Mechanism of hormonal action- Steroid and peptide hormones such as adrenaline glucocorticoids and insulin 3. Chemistry, physiological role and disorders of hormones of Pituitary, Hypothalamus and Thyrwid 4. Chemistry, physiological role and disorders of hormones of Pancreas 5. Chemistry. physiological role and disorders of hormones of Parathyroid 5. Chemistry, physiological role and disorders of hormones of Gonads, Placenta and Adrenals 7. Gastrointestinal hormones and their physiological role Credit — I: Nutrition 1. Balanced diet. Calorfic values of foods and their determination by bomb calorimeter. 2. BMR and factors affecting BMR. Specific dynamic action of foods. 3. Energy requirements and recommended dietary allowance (RDA) for children, adults, pregnant and lactating women, Sources of complete and incomplete proteins, Biological value of proteins. Role of essential fatty acids in human nutrition 5. Malnutrtion- Kwashiorkar, Marasmus and PEM, 6. Vitamins- sources, structure, biochemical roles, deficieney disorders of water and at soluble Vitamins; Bulk and trace elements-Ca, Mg, Fe, 1, Cu, Mo, Zn, Se and F. 7. Nutraceuticals: Obesity and starvation, Credit-1V: Cl al Biochemistry and Organ Function tests 1. Structure and funetions ofthe liver, Liver function tests- conjugated and total bilurubin in serum, albumin: globulin ratio, hippuric acid and bromsulphthalein tests, Serum enzymes in liver diseases- SGPT, GGT and alkaline phosphatase 2. Kidneys-structure of nephion and Mechanism of urine formation, Normal and abnormal constituents of urine 3. Biological buffers. Role of kidneys in maintaining acid-base and electrolyte balance inthe body Renal function tests- creatinine and urea clearance tests, phenol red test Agu!S. Biochemical tests for the diagnosis of heart diseases- HDL/LDL cholesterol, SGOT. LDH, CK. C-reactive protein, eardiae troponins, 6. Brain function tests - BEG 7. Glract test - Endaseopy References 1. ‘Textbook of Biochemistry and Human Biology Talwar, GP. and Srivastava. L.M., Printice Hall of India’ 2. Review of Medical Physiology-Ganong, MeGraw-Hill 5. Human Physiology ~Chatterjee.C.C, Medical Allied Agency 4. Textbook of Medical Physiology - Guyton.A.G and Hall.J.E., Saunders 5. William’s Textbook of Endocrinology ~ Larsen, R. P. Korenberg, H.N. Melmed. S. and Polensky. K. S. Saunders 6. Mammalian Biochemistry- White, A. Handler, P. and Smith, E. L, McGraw-Hill 7. Textbook of Human Nutrition- Bamyji, PralhadRaoand Reddy V. Oxford & IBH Publishers 8. Foods: Facts & Principle- Shakuntala andShadaksharaswamy. Wiley Ester Press 9. Essentials of Food and Nutrition — Swaminathan.M. Bangalore Press. 10, Human Nutrition and Dietetics. Davidson, S. and Passmore, J. R. ELBS, 11. A Textbook of Biochemistry: Molecular and Clinical Aspects. Nagini, S._ Scitech Publishers. 12. Tietz Fundamentals of Clinical Chemistry- Burtis, A. A. and Ashwood, E.R. Saunders imprint Elsevier Pub, 13. Textbook of Biochemistry with Clinical Correlations ~ Devlin.T.M.,Wiley — Liss 14, Textbook of Medical Biochemistry ~ ChatterjeaM.N. and Shinde.R, Jaypee Brothers Medical Publishers. 15, Textbook of Medical Biochemistry- Ramakrishnan, S., Prasannan, K. G. and Rajan, R. Orient Longman 16, Essentials of Food and Nutrition Swaminathan M. Bangalore Press Ge te »DSC -1E Paper - BS $04 A (Practicals): Physiology, Nutrition and Clinical Biochemistry (1 Credits; 2Hr/veek) Semest Estimation of hemoglobin in blood, Total count and Differential count ~ RBC and WBC Urine analysis for albumin, sugars and ketone bodies. Estimation of urinary creatinine. Estimation of total serum cholesterol. Estimation of vitamin C by 2, 6 - DCPIP method. Determination of iodine value of oil Determination of peroxide value of il References |. Experimental Biochemistry-A student companion-Beedu Sashidhar Rao and Vijay Deshpande 2. Laboratory Manual in Biochemistry- Jayaraman, J. Wiley Eastern 3. Biochemical Methods- Sadasivam,S and Manickyam,A. New Age Intemational PublishersDSC-1E Semester ~ V: Paper-BS S04 B (Theory): Cell Biology, Gene (4 Credits; SHriweek) Credit ~1: ell Biology Cell as basic unit of living organist Composition & functions of cell organelles 3, Cytoskeleton- Microfilaments, Microtubules & Intermedia structure of prokaryotic cell and eukaryote cell laments 4. Chromosome organization in Prokaryotes and Eukaryotes and. structure of chromosomes (Polytene and Lamp Brush) 5. Celleyele 6. Mitosis and Meiosis 7. Cell death ~ Apoptosis and Necrosis Credit ~ Il: Geneties |. Basie concepts of Genetics - Mendes laws ‘Non-Mendetian inheritance: Extra chromosomal inheritance (Paramoeeium & Drosophila) 3. Partial or incomplete dominance and Co-dominance 4. Maternal inheritance (Coiling in snails, Leber’s hereditary opt 5. Polyenie inheritance (Introduction to quantitative tats) 6. Ses hinked inheritance, X-linked recessive inheritance (colour blindness & Hemophilia). Concept ‘of Autosomal recessive and dominant inher 7. Linkage and recombination weuropathy (LLNON)). Credit— U1 |: Mutat and Mutager L._ Mutations (spontaneous induced, somati transversions) 2. Mutations (Silent, missem 3. Detection, selection & iso! 4. Estimation of mutation rates 5. Reversion and suppression of mutations 6. Mutagen ~ physical, chemical 7. Transposon mutagenesis, site-directed mutagenesis germinal, forward / reverse, transition wonsense, and frame shill mutations, conditional, leaky ) ion of microbial mutants Credit ~ 1V : Microbiology tntroduetion to bret history of microbiology. Classification of microorganisms. Mycoplasina Motility and sporulation Isolation and cultivation of bacteria. Selective media and enriched media, Grau's stainin, 4. Bacterial growth curve and kinetics of growth, Batch, continuous and synchronous cultures 3. Industrial uses of Asperillus niger. yeast and Spirulina, or, _ x) chuceu! fEX 27. Viral life cycles ~ TA (Lyte, & phage (Iytic and lysogenic), TMV, Retro viruses HIV, References iJ, Simmons, D, Peter Snustad; John |. Principles of Geneties by Eldon John Gardner, Mi Wiley 2. Moder Genetic Analysis Anthony JF Griffiths, William M Gilbert, Jeffrey H Miller. and Richard C Lewontin, Pub. W. H. Freeman 3. Lewin B. (Ed )¢ 1996) Genes, VIL edition, John Wiley and Sons, New York Cell and Molecular Biology, De Roberts and De Roberts, Lippincott & Wilkins Cell Biology by C. B, Pawar Principles of Genetics by R.H. TamarinMeGrawhill 4 6 7. Theory & problems in Genetics by Stansfield, Schaum out line series MeGrawhill 8 9 ‘Textbook of Microbiology ~ Ananthanarayan, R and JayaramPaniker, C.K... Orient Longman. Microbiology ~ Prescott.L.M.,Harley.J.P. &Klein.D.A, McGraw-Hill, robiology ~ PelezarJr,.M.J., Chan.E.C.S. and Krieg.N.R., Tata McGraw-Hill, 11. Textbook of Microbiology Dubey. R. C. and Maheshwari, D. K. S. Chand & Co, aaDSE-1E Semester ~ V: Paper-BS $04 B (Practicals): Cell Biology, Genetics and Microbiology (1 Credits; 2Hr/veek) Preparation of different stages of Mitasis and Meiosis, Problems on Monohybrid cross, Problems on dihybrid ratio in Drosophilaimaize, Linkage and Recombination, Sex linked inheritance and X-linked recessive inheritance 3. Sterilization methods and preparation of culture media, Isolation of pure cultures: (i) Streak plate method (ii) Serial dilution method, 4. Gram staining, 5. Motility of bacteria by hanging drop method 6. Bacterial growth curve, 7. Antibiotic sensitivity by paper dise method. References Essential practical handbook of Cell Biology & Genetics, Biometry and Microbiology: A Laboratory Manual by Debarati Das, Academic Publishers Microbiology — A Laboratory manual by Cappuccino and Sherman, Pearson Publications LPE. Experiments in Microbiology, Plant Pathology and Biotechnology by Aneja A. R., New Age Publications BEvi Semest DSE-1F ‘Semester ~ VI: Paper-BS 603 A (Theory): Molecular Biology and Imm (4 Credits; 4Hr/week) logy : DNA Replication, Experimental evidences to prove DNA as genetic material 1 2. Nature and structure of the gene. 3. DNA replication- models of rep! jon, Meselson-Stahl's experimental proof for semi conservative model. 4. Replication in prokaryotes - DNA polymerases 1, II and Ill of E coli, helicase, topoisomerases primase, ligase. 5. Bidirectional replication model. Okazaki fragments, synthesis. 6. Replication in Eukaryotes 7. Inhibitors of DNA replication. jging strands of DNA. leading and Ia n and Translation 11: Transcript ‘Transcription - RNA synthesis, RNA polymerases of prokaryotes and eukaryotes 1 2. Initiation, Elongation and Termination- tho dependent and rho independent. 3. Post-transcriptional modifications and Inhibitors of RNA synthesis. 4. Genetic code, Deciphering of genetic code, Nirenberg's and Khorana’s experiments, wobble hypothesis, degeneracy of genetic code. 5. Protein synthesis- structure of -RNA activation of amino acids (aminoacyl t-RNA synthetases). Ribosome structure 6. Initiation, elongation and termination of protein synthesis. Post- translational modifications and Inhibitors of protein synthesis. 7. Reguilation of prokaryotic gene expression- induction and repression. Lac operon Credit — 111: Immunology |. Organization of immune system. 2. Organs and cells of immune system. 3. Innate and acquired immunity. 4. Cell mediated and humoral immunity (T- and B- cells). 5. Classification of immunoglobulins, structure of IgG. Theories of antibody formation- clonal selection theory 6. Epitopes / antigenic determinants, Concept of haptens. Adjuvants, 7. Monoclonal antibodies and their applicat Credit — 1V: Immunotechnology nw Ou Antigen-antibody reactions- agglutination, immunoprecipitation, immunodiffusion Blood group antigens. Immunodiagnostics-RIA, ELISA.Vaccines and their classification, Traditional vaccines Modern vaccines- recombinant and peptide vaccines. Outlines of hypersensitivity reactions. Fundamentals of graft rejection and MHC proteins. References Molecular Biology of Cell- Alberts B. Bray, D. Lewis, J. Raff, M. Rober. K. and Watson D.Garland Publishing, Recombinant DNA and Biotechnology: A Guide for teachers- Helen and Massey. ASM Press. Genes VIII ~ Lewin. B, Oxford University Press Molecular Biology- Freifelder. D. Naroasa Pub. House Molecular Biology of the Gene- Watson. J.D., Baker, T.A, Bell, S.P.Gann.A, Levine, M and Losick R, Pearson Education. 6. Molecular Biotechnology- Glick, B. R. and Pasternak, J.. ASM Press 7._ Principles of Gene Manipulation: An Introduction to GE- Old, R. V. and Primrose, S. B Blackwell Sci. Pub. 8. Molecular Cell Biology- Lodish, H., Berk, A., Matsudaira, P.. Kaiser, C. A.. Krieger, M, Scott M P., Zipursky, S. L. and Damell, J. Freeman & Co, 9. Immunology. Tizard, I. R. Thomson Press. Immunology ~ Kindt.T.1., Goldsby.R.A. and Osbome.B.A., Freeman & Co jit’s Essential Immunology ~ Roitt..M. and Delves.P.J., Blackwell Science. DG saDSE-1F Semester ~ VI: Paper - BS 603 A (Practicals) : Molecular Biology and Immunology (1 Credits; 2Hr/week) 1. Isolation of DNA from onion/Plasmids Determination of purity of nucleic acids by UV-spectrophotometrie method. 3. Estimation of DNA by diphenylamine method. 4. Estimation of RNA by orcinol method 5, Electrophoresis of nucleic acids and visualization by ethidium bromide staining. 6. Agelutination: A, B, AB and O blood groups and Rh 7. ODD and Sandwich ELISA References |. Experimental Biochemistry-A student companion-Beedu Sashidhar Rao and Vijay Deshpande, Laboratory Manual in Biochemistry- Jayaraman, J. Wiley Eastern Biochemical Methods- Sadasivam,S and Manickyam,A. New Age International Publishers MES SassDSE-1F Semester ~ Vis Paper-BS 603 B (Theory): rDNA tech and Biotechnology (4 Credits: AHrhweck) Credit 1: rDNA technology I 1. Cloning strategies 2. Tools of DNA technology: Enzymes- Restriction endonucleases and ligases 5. Restriction mapping. 4. Polymerase chain reaetion- principle and applications 5 Outlines of blotting techniques-Southern, Norther and Westem 6. Molecular markers-RFLP,AFLP and RAPD. 7. DNA sequencing- Maxam Gilbert and Sanger’s methods Credit 1: r-DNA technology IT 1. Construction of e-DNA libraries. 2. Cloning vectors- Plasmids. Cosmids, and 4 phages 3. Hosts: Ecol! 4. Applications of gene cloning- production of insulin 5. Production of human growth hormone 6, Production of Br cotton 7. Edible vaccines IE: Plant and Animat Bi technology 1. Plant tissue culture and its applications 2. Plants as bioreactors and valuable chemical factories (production of bioactive compounds) 3. Transgenic plants, Crop improvement, Production of herbicide and insect resistant plants 4. Genetically modified crops ~ Arabidopsis, Golden rice, soybeans, Bt cotton. tobacco, potate. papaya, jatropha, 5. Animal cell cultures and its applications, 6. Animal cells as bioreactors. Molecular pharming; Production of vaccines, pharmaceutical proteins, recombinant hemoglobin and blood substituents 7. Transgenic animals Credit ~1 Microbial and Environmental Biotechnology |. Microbes as biocontrol agents, Microbial insecticides (Baculoviruses, Bacillus thurinigiensis and Bacillus sphaericus) 2. Bioremediation, Biodegradation of cellulose and lignocellulose, bio-sur emulsifiers 3. Microbial ore leaching and production of microbial fuels (hydrogen, methane) 4. Renewable and Non-renewable energy sources 5. Strategies involved in Municipal solid waste treatment, Treatment of industrial and domestic effluent (aerobic and anaerobic 6 Biomaterials as an alternative to non-degradable materials. Heavy Metal Accumulation, Biosorption 7. Heavy metal tolerance (ineluding mechanism) and its impget on momo | pu) ictamts and bioReferences Principles of Gene Manipulation: An introduction to GE ~ Old, R. and Primrose, S.6, Blackwell Sei. Pub Molecular Biotechnology Glick, BR and Paterak, JJ. Publish ASM Press Introduction to Biotechnology, William J. Thieman, Michael A. Palladino, Benjamin Cummings Publ Biotechnology- Arora, Himalaya pub. House Introduction to Environmental Biotechnology by A. K. Chatterji, PHI Learning Pvt. Li Animal Cells as Bioreactors - By Terence Gartoright, Cambridge Univ Press Text Book of Biotechnology - By H.K. Das (Wiley Publications) Introduction to Plant Tissue Culture - By M.K. Razdan (Oxford and IBH Publishing Company, New Delhi Industrial Microbiology by L.B. Casida »Semester — VI: Paper-BS 603 B (Practicals): r-DNA technology and Biote logy (1 Credits; 2Hriweek) Restriction mapping: -DNA with any two restriction enzymes strategies of Gene cloning 2. Preparation and transformation of competent cells 3: Preparation of MS medium and initiation of callus, Micropropagation of plants 4. Isolation of microbes from environment (Any source : soil, water, skin, bread, milk) 5. Efficacy testing for bio-ferttizers (nodulation test for rhizobia) and Efficacy testing for bio-pesticides 6. Microbial degradation of organic matter, Muricipal solid waste treatment and Waste water treatment 7. Produetion of hydrogen and methane References |, Molecular Cloning (Lab manual) by Maniatis T, Fritsch EF, Sambrook J, Volume -1, CSH 2. Microbial Biotechnology ~ A Laboratory Manual for bacterial systems by Das, Suri, Dash, HirakRanjan, Springer-Verlag 3. Plant Tissue Culture by Kalyan Kumar De 4. Biogas Vechnology by b.T. Nijaguna 5. Biotechnology procedures and experiments hendbook by S. Harisha, Infinity Science Press LLC. Ly Cw Now‘Semester IV: Optional Paper in place of Project (Theory) Biochemistry in health and disease (4 Credits: 4 hrs/week) Credit 1. Metabolic disorders Credit Credit ‘Amino acid metabolism Phenyketonuria, Alkaptonuria Carbohydrate Metabolism Galactosemia, Pentosuria Nucleic acid metabolism Gout, Leseh-Nyhan syndrome Lipid Metabolisin Gaucher's disease, Tay-sachs disease I. Genetic disorders Introduction to genetic diseases Chromosomal disorders- Down syndrome, Turner syndrome Hemoglobinopathi Thalassemia Gen Pr Sickle cell anaemia ie counselling al diagnosis Gene therapy HL, Endocrine disorders Introduction to endocrine disorders Endocrine organs- Pituitary glands Thyroid gland Parathyroid gland Panereas Ovaries, Testis Adrenal glands Diabetes ~ Type 1 & IT Thyroidism Polyey stic Ovaries Endometriosis, Contraceptives Addison's and Cushing syndrome 1V, Molecular Basis of Cancer Chemical Carcinogens ea chu Fundamental features of carcinogenesis Oncogenes, Tumor suppressor genes causing cancer Tumor biomarkers in bodily Muds. Mechanism of carcinogenesis ‘ew therapies in cancer Epigenetic mechanism in cancer.References: 1, Voets Principles of Biochemistry V Edition-2016 for Unit I & IL 2. Tietz Fundamentals of Clinical Chemistry-2010 for Unit IL 3. Harpers illustrated Biochemistry for Unit IV.B.Sc., CBCS for all Universities in Telangana (wet 2019-2020) B.Sc., BIOCHEMISTRY MODEL PAPER: THEORY For I & II Semesters Duration 3 hours Max. Marks 80 Section - A (Short Answer Type) Answer any eight of the following questions $x 4=32 Marks 1. Credit 4. Credit 5 6 7 Credit-tt oer 9, 10, CredittVv M1 12. Section - B (Essay Answer Type) Answer all Questions 4.x 12 = 48 Marks 9.(a) Credit (oR) (b) Credit 10, (a) Credit-ll (oR) (b) Credit 11. (a) Credit-Il (OR) (b) Credit-tll 12, Ce Chded (b) Credit-1VB.Se., CBCS for all Universities ‘Telangana (wef 2019-2020) : BSc., BIOCH ‘MISTRY fl MODEL PAPER PRACTICALS. For 1& I Semesters Duration: 3 hours Max. Marks 50. |, Write the Principles forthe following experiments (10 Marks) 2, Major Experiment (20 Marks) 3. Minor Experiment (10 Marks) 4. Viva-Voce and Record (10 Marks)