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Sab 4200839 Dat

This antibody is produced in rabbits against recombinant human acetylcholinesterase and purified using protein A. It specifically recognizes human acetylcholinesterase and may be used for techniques like immunoblotting, immunofluorescence, and ELISA. Acetylcholinesterase is an enzyme found in neuromuscular junctions and brain synapses that hydrolyzes acetylcholine to terminate signal transmission. This antibody can help detect acetylcholinesterase levels and is useful for studying neurodegenerative diseases and muscle weakness conditions.

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37 views

Sab 4200839 Dat

This antibody is produced in rabbits against recombinant human acetylcholinesterase and purified using protein A. It specifically recognizes human acetylcholinesterase and may be used for techniques like immunoblotting, immunofluorescence, and ELISA. Acetylcholinesterase is an enzyme found in neuromuscular junctions and brain synapses that hydrolyzes acetylcholine to terminate signal transmission. This antibody can help detect acetylcholinesterase levels and is useful for studying neurodegenerative diseases and muscle weakness conditions.

Uploaded by

mohammad293
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Anti-Acetylcholinesterase (AChE)

antibody produced in rabbit


IgG fraction of antiserum

Product Number SAB4200839

Product Description Erythrocyte AChE (AChE-H) is a membrane encored


Anti-Acetylcholinesterase (AChE) antibody is AChE splicing variant found in RBC. It belongs to the
developed in rabbits using recombinant human AChE, glycosylphosphatidylinositol (GPI) protein family and
expressed in HEK 293 cells (GeneID: 43). Whole carries the Yta blood group antigen. Erythrocyte AChE
antiserum is purified using protein A immobilized on can be found in plasma circulating vesicles originated
agarose to provide the IgG fraction of antiserum. from aged erythrocytes, and therefore is considered as
a biomarker for RBC membrane integrity. Furthermore,
Anti-Acetylcholinesterase (AChE) antibody specifically Erythrocyte AChE activity is also a marker for
recognizes human AChE. The antibody may be used in inflammation and other diseases including essential
various immunochemical techniques including hypertension, glaucoma, ALS, neurotoxicity, and
immunoblotting, immunofluorescence, and ELISA. Hirschsprung’s disease.4,7-8
Detection of the AChE band by immunoblotting is
specifically inhibited by the immunogen. Reagent
Supplied as a solution in 0.01 M phosphate buffered
Acetylcholinesterase (AChE) is a member of the saline, pH 7.4, containing 15 mM sodium azide as a
/-hydrolase-fold proteins superfamily which is preservative.
composed of structurally related proteins with a great
diversity in their catalytic, recognition, adhesion, and Precautions and Disclaimer
chaperone functions. 3D structure studies of For R&D use only. Not for drug, household, or other
/-hydrolase revealed a variety of folding patterns uses. Please consult the Safety Data Sheet for
suggesting associations through oligomerization information regarding hazards and safe handling
dependent functions.1 practices.

AChE is a tissue specific serine hydrolase expressed in Storage/Stability


several variants at neuromuscular junctions (NMJs),2 For continuous use, store at 2–8 C for up to one
cholinergic brain synapses,2-3 or erythrocytes (Red month. For extended storage, freeze in working
Blood Cells, RBC).4 In the synaptic cleft, the main aliquots. Repeated freezing and thawing is not
function of AChE is to ensure rapid hydrolysis of recommended. If slight turbidity occurs upon prolonged
acetylcholine (ACh) released from motor nerve endings storage, clarify the solution by centrifugation before
into choline and acetic acid, thus preventing use. Working dilution samples should be discarded if
postsynaptic muscle ACh receptors (AChR) not used within 12 hours.
hyperactivation and termination of the signal
transmission.2-3,5 Product Profile
Immunoblotting: a working dilution of 1:1000-1:2000 is
AChE and Butyrylcholinesterase (BChE) inhibitory recommended using whole extracts of Jurkat cells.
drugs or peptides are being used in several
neurodegenerative diseases including Alzheimer’s Immunofluorescence: a working dilution of 1:1000-1:2000
disease and pathological muscle weakness such as is recommended using human A431.
Myasthenia Gravis. These AChE inhibitors increase
synaptic ACh levels and hence improve the cholinergic Note: In order to obtain best results in various
function in the brain.2-3,5-6 techniques and preparations, it is recommended to
determine optimal working dilutions by titration test.
References
1. De Jaco, A. et al., FEBS J., 279, 4293-305 (2012). 5. Petrov, K.A. et al., Sci. Rep., 8, 304 (2018).
2. Prasasty, V. et al., Molecules, 23, pii: E2344 6. Dos Santos, T.C. et al., Front. Pharmacol., 9, 1192
(2018). (2018).
3. Petrov, K.A. et al., Front Pharmacol., 9, 766 7. Chen, A. et al., Environ. Sci. Technol., 46, 1828-33
(2018). (2012).
4. Saldanha, C., Molecules, 22, pii: E1499 (2017). 8. Gupta, V.K. et al., Enzyme Res., 2015, 370705
(2015).

VS,DR,OKF,MAM 06/19-1

2019 Sigma-Aldrich Co. LLC. All rights reserved. SIGMA-ALDRICH is a trademark of Sigma-Aldrich Co. LLC, registered in the US and other
countries. Sigma brand products are sold through Sigma-Aldrich, Inc. Purchaser must determine the suitability of the product(s) for their
particular use. Additional terms and conditions may apply. Please see product information on the Sigma-Aldrich website at
www.sigmaaldrich.com and/or on the reverse side of the invoice or packing slip.

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