Bharati Vidyapeeth's College of

EngineeringNew Delhi-110063

Department of Applied Sciences

Course File
Academic Year: 2021-22

Semester: II

Subject name: ENVIRONMENTAL STUDIES LAB

Subject Code: BS-162

Faculty Name: Dr. AMREETA PREETAM

 S.
No. CONTENTS
1 Vision and Mission of the Institute
2 Vision and Mission of the Department, PEOs, POs, PSOs
Course Description with CO's, CO-PO/PSO Mapping, Syllabus, Practical
3 Plan and Evaluation Scheme
Common for
4 Practical Manual
every single
Innovative Teaching-Learning Methods (Details of NPTEL/Other online course
5 resources used)
6 Lab experiment with CO Mapping (Current Semester)
7 Evaluation scheme
8 Course End Survey (Format Only)
9 CO Attainment

10 Sample Evaluated Lab files.

11 Attendance Register
 1. Vision &Misson of Institute

Vision & Mission of Institute

Vision of Institute: To be an institute of excellence that provides quality technical education and
research to create competent graduates for serving industry and society.

Mission of Institute
M1: To impart quality technical education through dynamic teaching-learning environment.

M2: To promote research and innovations activities which gives opportunities for life-long learning in
context of academic and industry.

M3: To build up links with industry-institute through partnerships and collaborative developmental
works.

M4: To inculcate work ethics and commitment in graduates for their future endeavours to serve the
society

2. Vision & Mission of Department

Department of Applied Sciences

Vision:
The department aspires to be a center of excellence in education in basic sciences and technology
with ethical and social values.

Mission:
DM-1: To provide quality education through professional, problem-driven and interdisciplinary
teaching methodology

DM-2: To make students sensible in terms of ethical and social values in pursuing their
education
Program Educational Objectives (PEO):
PEO1: Fundamental Knowledge: Under graduates will be skilled in the fundamental
concepts of basic sciences necessary for success in industry or in engineering practices as well as
advanced study.
PEO2: Specialization: Under graduates will be prepared to pursue career choices in all branches
of engineering or related interdisciplinary fields that will benefit from a strong background in
applied sciences and engineering.
PEO3: Design Skills: Under graduates will be prepared with problem solving skills,
laboratory skills, and design skills for technical careers in solving critical problems.
PEO4: Professional Skills: Under graduates will be prepared as an effective team member with
good communication and teamwork skills as well as an appreciation for ethical behavior
necessary to thrive in their careers.
PEO5: Self-Learning: Under graduates will be prepared to continue their
professional development through continuous education and personal development
experiences based on their awareness of library resources and professional societies, journals,
and meetings, conferences, seminars, workshops, expert talks, industry interactions, etc.
PEO6: Sustainability: Under graduates would be able to maintain the environmental
serenity along with keeping pace with the ever changing scenario of the industry.

Program Outcomes (POs):

1. Engineering knowledge: Apply the knowledge acquired in mathematics, science, engineering
for the solution of complex engineering problems.
2. Problem analysis: Identify research gaps, formulate and analyze complex engineering
problems drawing substantiated conclusions using basic knowledge of mathematics, natural
sciences and engineering sciences.
3. Design/development of solutions: Design solutions for the identified complex engineering
problems as well as develop solutions that meet the specified needs for the public health and
safety, and the cultural, societal, and environmental considerations.
4. Conduct investigations of complex problems: Use research-based knowledge and research
methods, including design of experiments, analysis and interpretation of data and synthesis of the
information to provide valid conclusions.
5. Modern tool usage: Work on the latest technologies, resources and software tools including
prediction and modelling to complex engineering activities with an understanding of their
limitations.
6. The engineer and society: Apply the basic acquired knowledge to measure societal, health,
safety, legal and cultural issues and identifying the consequential responsibilities relevant to the
professional engineering practice.
7. Environment and Sustainability: Comprehend the impact of the professional engineering
solutions in context of society and environment and demonstrate the need and knowledge for
sustainable development
8. Ethics: Apply ethical principles and commit to professional ethics and responsibilities and
norms of the engineering practice
9. Individual and team work: Function effectively as an individual, and as a member or leader
in diverse teams, and in multidisciplinary settings.
10. Communication: Communicate effectively on complex engineering activities with the
engineering community and with society at large, such as, being able to comprehend and write
effective reports and design documentation, make effective presentations, and give and receive
clear instructions.
11. Project management and finance: Demonstrate knowledge and understanding of the
engineering and management principles and apply these to one’s own work, as a member and
leader in a team, to manage projects and in multidisciplinary environments.
12. Life-long learning: Recognize the need for, and have the preparation and ability to engage
in independent and life-long learning in the broadest context of technological change.

3.Subject Syllabus/Lab Plan

PaperCode: BS-161 / BS-162 Paper: Environmental Studies Lab. L P C

- 2 1

LIST OF EXPERIMENTS

1. Determination of the soil moisture content in the given soil sample.

2. Determination of Total Dissolved Solids (TDS) of water sample.
3. Determination of moisture content of soil sample.
4. Determination of dissolved oxygen (DO) in the water sample.
5. Determination of Biological oxygen demand (BOD) in the water sample.
6. Determination of Chemical oxygen demand (COD) in the water sample.
7. Determination of Residual Chlorine in the water sample.
8. Determination of ammonia in the water sample.
9. Determination of carbon dioxide in the water sample.
10. Determination of pH and conductivity of soil/sludge samples.
11. Base catalyzed aldol condensation by Green Methodology.
References:

1. Vogel’s Text Book of Quantitative Chemical Analysis by G.H. Jefferey, J. Bassett, J. Mendham, and
R.C. Denney, Logmaan Scientific & Technical, 1989

2. Essentials of Experimental Engineering Chemistry by S. Chawla, Dhanpat Rai & Co., 2008.

3. Experiments in Applied Chemistry by S. Ratan, S.K. KAtaria & Sons, 2003.

4. Practical Chemistry by O.P.Pandey, D. N. Bajpai and S. Giri, S.Chand & Co., 2005.

5. Engineering Chemistry with Laboratory Experiments by M. S. Kaurav, PHI Learning Pvt. Ltd., 2011.
6. Laboratory Manual on Engineering Chemistry by S. K. Bhasin, and Sudha Rani, Dhanpat Rai &Co.,
2006.

Note:

1. At least 8 Experiments out of the list shall be performed by the students. Teachers may introduce
new experiments for the class in addition to above.

4.Practical Manual

EXPERIMENT-1

AIM: To estimate the soil moisture content in the given soil sample.
APPARATUS: Watch glass; Drying oven, Spatula, Desiccator
THEORY: Water plays a very significant role in soil growth relationship. It forms major part
of plant itself, essential for physiological activities, acts as a solvent and nutrient carrier and
maintains turgidity of the plants. In fact water is a regulator of physical, chemical and biological
activities in the soil.
Water in a soil may be measured in a number of ways: Gravimetric, Volumetric method,
Neutron scattering, Soil moisture tension and Electrical conductivity. However gravimetric
method is the simplest and the most widely used method. In this the weighed Soil sample is
placed in an oven at 105oC and it is dried up to constant weight. The weight difference is
considered to be water present in soil sample.

Percent moisture = (Loss in weight /weight of soil) × 100

PROCEDURE

1. Weigh the empty watch glass.

2. Put 10 g soil sample on the watch glass and weigh immediately to avoid loss in moisture
content.
3. Place the watch glass in a preheated oven at 105oC for 1 h.
 1
4. Keep the watch glass in a desiccator for cooling for at least h.
2
5. Now weigh the watch glass along with the oven dry soil.

OBSERVATIONS
Weight of empty watch glass = x
Weight of watch glass + moist soil = y
Weight of watch glass + oven dried soil = z
CALCULATIONS
Moisture content in soil = ( ( y  z ) )
Weight of moist soil = ( ( y  x) )
 yz
Percentage moisture in soil =    100
 yx
RESULT: The amount of moisture present in soil sample is………..

EXPERIMENT-2

AIM: Determination of Total dissolved solids in water/ effluent sample.

THEORY: Dissolved solids" refer to any minerals, salts, metals, cations or anions dissolved
in water. Total dissolved solids (TDS) content of drinking water varies between 20-1000 mg L-1,
and it comprises mainly of inorganic salts (principally calcium, magnesium, potassium, sodium,
bicarbonates, chlorides and Sulphates) and some small amounts of organic matter and dissolved
gases.
TDS in drinking-water originates from natural sources, sewage, urban runoff, industrial-
wastewater, and chemicals used in the water treatment process, and the nature of the piping or
hardware used to convey the water, i.e., the plumbing.

Table 1: Total Dissolved Solids (TDS) in some water samples.

Water Type TDS (mg L-1)

Sea Water 35,000
Potable Water <500
Irrigation Water 0-2000

PROCEDURE

1. Take the empty weight of evaporating dish/crucible.

2. Measure accurately 100ml of well mixed water/effluent Sample and filter the sample
through the whatman filter paper.
3. Transfer filtrate to a preweighed evaporating dish/crucible and evaporate to dryness on a
hot plate.
4. Dry for at least 1 h in oven at 180oC.
5. Cool in a desiccator and take the final weight. Calculate the total dissolved solids (TDS)
using the given equation.

CALCULATION:

Total dissolved solids (mg L-1) = ( A  B)  V

Where A = weight of dried residue + dish
B= weight of dish and
V = volume of sample in litre
RESULT: The amount of total disso1ved solids in a given water sample is …….mg L-1
SIGNIFICANCE: An elevated TDS indicates the following:
1. The concentration of the dissolved ions may cause the water to be corrosive, salty or
brackish taste, results in scale formation, and interfere and decrease efficiency of hot
water heaters; and
2. Many contain elevated levels of ions that are above the Primary or Secondary Drinking
Water Standards, such as: an-elevated level of nitrate, arsenic, aluminium, copper, lead,
etc.
DISSOLVED OXYGEN AND OXYGEN DEMAND

One of the most important water quality parameter is the amount of dissolved oxygen
(DO) present. Oxygen is poorly soluble in water. The amount of oxygen in water depends on
physical, chemical and biochemical activities taking place in water. The solubility of dissolved
oxygen in water at saturation at any temperature and pressure is given by Henry’s law. The –
saturation value of DO in water is of the order of 8-15 mg L-1, depending upon temperature.
Minimum amounts required for aquatic life for a healthy fish population is 6-8 mg L-1.

Significance of the determination of DO

 In aquatic life, as DO drops, fish and other species are threatened and may get killed.
 Fall in DO levels causes undesirable odours, tastes and reduce the acceptability of water
for domestic use.
 In steam generation, DO is one of the most important factors causing corrosion of the
boiler material.
There are several measures of oxygen demand commonly used. Two of these are:

(i) Biochemical oxygen demand (BOD)

(ii) Chemical oxygen demand (COD)

BIOCHEMICAL OXYGEN DEMAND

When biodegradable organic matter is released into water, the microorganisms feed on
the wastes and break it down into simpler organic or inorganic substances. If the decomposition
takes place in the presence of oxygen i.e aerobically the non-objectionable, stable end products
are formed.

On the other hand if insufficient oxygen is available, the decomposition takes place
anaerobically. The micro-organisms causing the decomposition of the organic matter in absence
of oxygen are entirely different from aerobic bacteria and produce highly objectionable end
products including H2S, NH3 and CH4.
 The
amount of oxygen required by micro-organisms to oxidize organic wastes aerobically is called
biochemical oxygen demand (BOD).
Actually when sufficient O2 is present, i.e. aerobic systems, oxygen is the oxidizing agent to
convert organic waste into CO2 and H2O. Similarly the inorganic materials also oxidized. The
products obtained are not harmful.

However, if sufficient oxygen is not present (anaerobic conditions), organic matter is oxidized by
nitrate to form undesirable products.
5CH3COO- + 8NO3-  8H+  
Bacteria
 5CO2  5HCO3  4N2  9H2O
CH3COO- + NO3-  
Bacteria
 CO2  HCO3  NH3
The products in this case like NH3 etc. give bad odour to water.
Example NH3give smell of rotten fish. Similarly under anaerobic conditions, SO24 , PO34 , CO2
are reduced to H2S, SH-, phosphine, methane etc. giving rotten egg smell or suffocating smell.
Therefore oxygen demanding wastes are termed as water pollutants because they deplete its
dissolved oxygen. Thus sufficient oxygen may be present or supplied to maintain aerobic
conditions and hence prevent bad odour; taste and colour.

CHEMICAL OXYGEN DEMAND

Although BOD test is applicable to organic wastes, there are many drawbacks. Certain organic
materials are not biodegradable and hence can give wrong conclusions that less organic matter is
present because BOD of such water samples will be low. In this case COD, Chemical Oxygen
Demand reveals the real organic content present. Here the oxidation of organic substances
present in water is done chemically.
COD, is the amount of oxygen required by organic matter in a sample of water for its oxidation
by a strong chemical oxidizing agent such as K2Cr2O7.
Since in COD determination, the organic matter, both biologically oxidizable (glucose) and
biologically inert (cellulose) is completely oxidized to CO2 and H2O; COD values are greater
than BOD values.
Experiment NO. 3
AIM: Determination of dissolved oxygen in sample of water.
APPARATUS: Burette, Pipette, Conical flask, Beaker etc.
CHEMICALS: Na2S2O3 (N/40), MnSO4 solution, KI, Starch, Conc.H2SO4.
THEORY: Oxygen itself is not a pollutant in water but its deficiency is an indicator of several
types of pollution in water.
Dissolved oxygen (DO) is determined by Winkler’s method or iodometric titration. The
dissolved oxygen in water oxidizes KI and an equivalent amount of iodine is liberated. This
iodine is titrated against a standard hypo solution. However since dissolved oxygen in water is in
molecular state and is not capable of reacting with KI, therefore an oxygen carrier such as
manganese hydroxide is used.
The method involves introducing a conc. solution of MnSO4, NaOH and potassium iodide, azide
reagent, into the water sample. The white precipitate of Mn(OH)2 which is formed, is oxidized
by oxygen in water sample to give a brown precipitate of basic manganese oxide.
This MnO(OH)2 , in acidic medium dissolves and liberates free iodine from the added KI in an
equivalent amount of dissolved oxygen in water sample. This liberated I2 is then titrated against
Na2S2O3 solution using starch as indicator. The reactions involved are:

MnSO4  2NaOH  Mn(OH)2 +Na 2SO4

4Mn(OH)2 +O2  2MnO (OH)2
2MnO (OH)2  H2SO4  MnSO4  2H2O +O
2KI  H2SO4  O  K 2SO4  H2O +I2
2Na 2S2O3  I2  Na 2S4O6  2NaI

The nitrites present in water, interfere with the titration as these can also liberate I2 from KI.
2HNO2  H2SO4  2KI  2NO  K 2SO4  2H2O  I2
Thus to destroy nitrite, sodium azide is used.
2NaN3  H 2SO4  2HN3  Na 2SO4
HNO2  HN3  NO2  N 2  H 2 O
PROCEDURE:
1. A known amount of sample water (say 250 ml) is taken in a stoppered bottle avoiding contact
with air: -
2. Add 0.4 ml of MnSO4 solution to it by means of a pipette, dipping the end well below the
surface of water. Also add 2 ml of alkaline iodide-azide solution to it.
3. Stopper the bottle and shake thoroughly. Allow the brown precipitates of MnO(OH)2 formed
to settle down.
4. When some portion of the liquid below the stopper is clear, add 2 ml of conc. H2SO4 with the
help of pipette. Stopper and mix till the precipitate is completely dissolved. The characteristic
brown colour of iodine is produced.
5. Transfer 100 ml of the above solution in a 250 ml flask with a pipette. Titrate the liberated I2
with standardized sodium thiosulphate solution until the sample solution becomes pale yellow.
6. Add 2 ml of starch solution, the solution will turn blue.
7. Continue titration till the blue colour disappears.
8. Repeat to get concordant reading.

OBSERVATIONS
Volume of the water sample taken for titration = 100 ml

Observation Table:

S. No. Burette Readings Volume of the titrant

Volume of the solution taken in the Initial Final used
titration flask Reading Reading (Final-Initial reading)

1
2
3

CALCULATIONS
Hypo = Oxygen in water

N1V1 = N2V2
 N1  V1  N 2 100
N1V1
N2 
100
Strength of dissolved oxygen = N2  Equivalent Weight

N1V1
  8gL1
100
N1V1
  8 1000 mgL1
100
RESULT: The amount of dissolved oxygen in water =……ppm
PRECAUTIONS
1. The water should be taken in the stoppered bottle very carefully without trapping air bubbles
which could raise oxygen level by aerating the sample.
2. MnSO4 and alkaline iodine-azide solutions are added to the water sample just below the
surface of water.
3. Whole of the precipitate of MnO(OH)2 should be dissolved in H2SO4.

Instructions for the Lab Assistant:

1. MnSO4 solution: Dissolve50g of MnSO4 in distilled water and make up the volume to 1
L.
2. Alkaline potassium iodide azide reagent: Dissolve 40 g of NaOH, 20 g of KI in distilled
water-, add 0.5 g of reagent grade sodium azide to the cooled solution and make up the
volume to 100 ml
3. N/40 Na2S2O3 solution: Dissolve 6.20 g of Na2S2O3 in distilled water and make up this
volume to 1 L. Standardize it with N/40 K2Cr2O7 solution.

EXERCISE

1. Why MnSO4 is used in the above determination?

Ans: Since oxygen in water is in molecular form and is not capable of reacting with KI, therefore
an oxygen carrier such as manganese hydroxide is used. This in turn is prepared by MnSO4 and
NaOH.
2. Why azide is added to the reaction mixture?
3. What is the significance of the above determination?
Ans: Measurement of D.O. is necessary because
  It causes corrosion in boiler feed waters.
 Conc. of dissolved oxygen is a good indicator for polluted Waters.
 DO is necessary for life of fish and other aquatic organisms.
Note: Sodium azide is poisonous. It may also explode if exposed to heat.
EXPERIMENT NO. 4

AIM: To determine Biological Oxygen Demand (BOD) of a given sample of water.

APPARATUS: Burette, Pipette, Conical flasks, Beakers.
CHEMICALS: MgSO4 solution (22.5 g/L), CaCl2 (27.5 g/L), FeCl3 solution (0.25 g/L),
phosphate buffer Solution; BOD bottles, Na2S2O3.
THEORY: The BOD in a water sample is directly related to the extent of pollution by sewage or
other oxygen demanding waste. If the oxygen in water is less than Biological Oxygen Demand,
the decomposition of microorganisms-takes-place-anaerobically (in absence of oxygen) and
hence produce highly objectionable end products including H2S, NH3 and CH4. Therefore
sufficient oxygen must be present or supplied externally to maintain aerobic conditions so that
organic matter decomposes in to acceptable products. To know the amount to oxygen required
by the given Sample, the determination of BOD is necessary.
Polluted waters will continue to absorb oxygen for many months, till the oxidation gets
completed and hence it is not practical to determine this ultimate oxygen demand which would
require an extended period of time. As a result, it has become standard practice to simply
measure the oxygen over a shorter period of 5 days. This is known as 5-day BOD test. BOD5 is
the total amount of oxygen consumed by micro-organisms during the first five days of
biodegradation.
In its simplest form BOD5 would involve diluting known volume of sample water with a
known volume of pure water, whose oxygen content is already known: Let it be D1. This is kept
for 5 days at 20 oC in a stoppered bottle, away from light. The dissolved oxygen is measured
after the period of incubation. Let it be D2. The difference between the original oxygen content
and residual oxygen content will indicate the oxygen consumed by the water sample in five days
in oxidizing the organic matter present in water sample. Thus BOD5 is given by:
Volume of sample after dilution
BOD5  D1 -D2
Volume of sample before dilution

Where, D1= D.O. of the sample in mg L-1 at the start of experiment

D2 = D.O. of the sample in mg L-1 after 5 days.
= Loss of oxygen in mg L-1× Dilution factor
The dilution of the waste water sample with pure water is necessary because waste water
contains more of oxygen demanding material and hence the dissolved oxygen may not be
sufficient to decompose it aerobically.
PROCEDURE:
Preparation of dilution water:
1. Take 1000 ml of distilled water in bottle. Add l ml each of phosphate buffer (pH = 7.2), CaCl 2
(27.5 g L-1) MgSO4 (22.5 g L-1) and FeCl3 (0.25 g L-1).
2. Water is aerated bypassing compressed air through diffusion tube until it is completely
saturated.
Determination
3. Take about 5ml of the .sample and dilute it to 300 ml with dilution water prepared above (or
any appropriate dilution).
4. The diluted sample is taken in two bottles filled up to the neck.
5. The dissolved oxygen in one bottle is determined immediately and in the other bottle after five
days of incubation. The procedure for determining the DO is the same given in experiment
number 3.
OBSERVATION
(i) Determination of. D. O. in bottle No. 1

S. No. Volume of the solution taken in Burette Readings Volume of the

titration flask (ml) Initial Final titrant used (final-
Reading Reading initial reading) (ml)
1
2
3

(ii) Determination of. D. O. in bottle No. 2 (after incubation for 5 days)

S. No. Volume of the solution taken in Burette Readings Volume of the

titration flask (ml) Initial Final titrant used (final-
Reading Reading initial reading) (ml)
1
2
3
Volume of sample taken for each titration = 100 ml
N
Normality of Na2S2O3 used =
100
CALCULATIONS:
Volume of the sample before dilution = 5 ml.
Volume of the sample after dilution = 300 ml.
(i) D.O in bottle -1
Hypo = O2 in sample
N1V1 = N2V2
1
 V1  N 2 100
100
V1
N2 
10000
Strength of dissolved O2 = N2× Equivalent Weight
V1
N2   0.8g L1  0.8V1 ppm
10000
(ii) D.O in bottle-2
Similarly find D.O in bottle-No-2
Let it be D2
Volume of sample after dilution
(iii) BOD  D1 -D2  mgL1
Volume of sample before dilution
RESULT: BOD of the given water sample =…... ppm
Instructions for the Lab Assistant
Prepare the solutions as in the case of determination of dissolved oxygen in experiment no 3.

EXERCISE
1. What do you mean by BOD?
2. What is BOD5 test?
3. What is the significance of BOD determination?
4. Why bad odour is developed in water if insufficient dissolved oxygen is present in water?
5. How is diluted water prepared?
Ans: It is prepared from distilled water by seeding with a small amount of domestic waste
water to provide a mixed population of bacteria or it can be prepared as given in the
experiment.
 6. Why, while preparing diluted water is added, 1 ml each of phosphate, FeC13, CaC12,
MgSO4 etc.?
Ans: Phosphate is added to maintain a pH of 7 and it also serves as a nutrient. Salt like FeC13
is added to supply nutrients to bacteria and Ca2+ Mg2+ is added to serve as trace elements
needed for growth of bacteria.
Note. The dilution depends upon the nature of the sample. For example 1-4% for industrial wastes,
1-5% for raw and settled sewage, 25-100% for polluted water, 2-5% for domestic waste water).
EXPERIMENT NO. 5
AIM: To find the chemical oxygen demand (COD) of a waste water sample using K2Cr2O7
APPARATUS: Burette, pipettes, conical flask, beakers, round bottom flask, condenser, water
bath
CHEMICALS: N/4 K2Cr2O7, N/4 Mohr's salt solution, ferroin indicators, sample solution,
HgSO4, silver sulphate reagent.
THEORY: The chemical oxygen demand is defined as the amount of oxygen used while
oxidizing the organic matter content of a sample with strong chemical oxidant-under-acidic-
condition.
A known amount of K2Cr2O7 is added to the measured amount of the sample and mixture is
boiled with conc. H2SO4. The organic matter gets completely oxidized to produce CO2and H2O.

 y z y
Cx H y Oz   x    O2  xCO2  H 2O
 4 2 2

The excess of K2Cr2O7 remained after the reaction is titrated against standard Mohr's salt-
solution using ferroin as indicator. The dichromate consumed is calculated which gives the
oxygen required for the oxidation of organic matter.

INDICATOR: Ferroin

END POINT: Blue to wine red or (colourless to red)

PROCEDURE:

1. Take 50 ml of the sample in a round bottomed flask and add 1 g of HgSO4 and some broken
porcelain pieces.
2. Immerse the flask in cold water and slowly add 75 ml silver sulphate reagents with continuous
shaking. (As this is an exothermic process that is why the flask is immersed in cold water)
3. Now add 25 ml of K2Cr2O7 (0.25N) to this solution and mix the contents of the flask.
4. Attach the reflux condenser and reflux for 2 h.
5. Wash the condenser with distilled water in to the flask. Cool and dilute to about 300 ml by
distilled water.
6. Add 2-3 drops of ferroin as indicator and titrate against 0.25N Mohr’s salt solution till the end
point, i.e. from blue to wine red.
7. Record the volume of Mohr’s salt solution used. Let it be X ml.
8. Perform a blank titration using distilled water in place of sample solution. For this, take 50 ml
of distilled water in round bottom flask, add the same amount of reagents and reflux for 2 h.
Titrate it in the same way as is done with the sample water.
9. Record the volume of Mohr's salt solution used. Let it be Y ml.
OBSERVATIONS:
Volume of the sample taken = 50 ml.
Observation Table
(a) For sample solution

S. N. Volume of the solution Burette Readings Volume of the

taken in the titration flask titrant used (final-
(ml) Initial Final Reading initial reading) (ml)
Reading
1
2
3

(b) For distilled water (blank titration)

S. N. Volume of the solution Burette Readings Volume of the

taken in the titration flask titrant used (final-
(ml) Initial Final Reading initial reading) (ml)
Reading
1
2
3

Volume of Mohr's salt so1ution used in case of sample solution = X ml (say)

Volume of Mohr's salt solution used in case of distilled water = Y ml (say)
 Volume of K 2Cr2O7 used to oxidize 
 the organic matter present in the water sample    Volume of Mohr's salt solutionequivalent to K 2Cr2O7   [Y-X]ml
 

N1V1 =N2 V2
(Sample) (Mohr’s salt solution)

1
N1  50 = (Y-X)
4
1
N1 = (Y-X)
4  50
Therefore, COD = N1 × Equivalent Weight of O = N1× 8 g L-1
RESULT: The. COD of' the given sample is N1× 8 × 1000 mg L-1
PRECAUTIONS:
1. The addition of Ag2SO4-H2SO4 to the sample should be done slowly with shaking and cooling
during mixing.
2. It should always be added through the open end of the condenser.
3. Smaller volume of the sample should be taken.
Notes: 1. HgSO4 is used to remove the chloride ions which may interfere because of their oxidation
under the experimental conditions.
2. Ag2SO4 is used as a catalyst to achieve the oxidation of straight-chain aliphatic hydrocarbons and
low molecular weight fatty acids.

Instructions to the Lab Assistant

Provide the following:
N
1. K2Cr2O7 solution: Dissolve 12.25g of K2Cr2O7 in distilled water and make up the
4
volume to 1000 ml.
N
2. Ferrous Ammonium sulphate (Mohr's salt solution):- Weigh 98 g of Mohr's salt and
4
transfer in flask having a few drops of conc. H2SO4. Add distilled water to dissolve it.
Make up the volume to 1000 ml.
3. Ferroin indicator: It can be purchased from the market as such or can be prepared in the
lab. Dissolve 3.7125 g of o-phenanthroline monohydrate with 1.6375 g of FeSO4. 7 H2O
in distilled water and make up the volume to 250 ml.
 4. HgSO4 (solid)
5. Silver sulphate reagent: Dissolve 5g of AgSO4 in 500 ml of conc. H2SO4.

EXERCISE

1. Define COD?
2. What is the significance of COD determination?
Ans: The COD is widely used for measuring the polluting properties of domestic and industrial
wastes.
3. What is the structure of ferroin indicator?
Ans. Ferroin is a ferrous complex of the organic compound 1, 10-phenanthroline

1, 10- Phenanthroline Ferrous 1, 10-Phenanthroline (Ferroin)

 EXPERIMENT NO. 6

RESIDUAL CHLORINE

Ingestion of pathogens (disease causing bacteria) not only in drinking water, but also from water
that makes it into person’s mouth through food, utensils and hands.
The process of destroying these pathogenic bacteria and micro-organism is known as
sterilization or disinfection. The chemicals used for the above purpose are called disinfectants.
The various methods used for disinfection of water include, boiling, addition of bleaching
powder, chlorine, UV radiation etc. However chlorination of water is widely used for
disinfecting water supply since chlorine is a powerful oxidizing agent and is cheaply available.
Chlorine is applied to water in its elemental form or as hypochlorite (bleaching powder).

Disinfecting Action of Chlorine: Cl2 reacts with H2O to form hypochlorous acid or
hypochlorite ions which have immediate disastrous effect on micro-organisms.
Cl2 +H2O 
pH < 5
 HOCl+HCl

The hypochlorous acid is unstable and may dissociate as

HOCl 
pH > 8
pH < 7
 H + + OCl-
Hypochlorite ion

All the three forms viz. HOCl, OCl- and Cl2 existing in sample water are termed as free chlorine.
When HOCl react with NH3 likely to be present in water, it forms chloramines.
NH3  HOCl  NH 2Cl+H 2 O
NH 2Cl + HOCl  NHCl2 +H 2O
NHCl2  HOCl  NCl3 +H 2 O
These chloramines also have disinfecting properties and act as chlorine in this form is called
combined chlorine.
The chlorine is added to water up to the break point. The break point chlorination of
water is defined as chlorination to such an extent that micro-organisms as well as bad tastes and
odour present in water are destroyed.
However, excess of free chlorine in drinking water is undesirable. An overdose of chlorine
imparts unpleasant tastes and is also injurious to health.
 Hence chlorine determination is necessary in those samples of water that have been
treated with chlorine or hypochlorites (bleaching powder) so that necessary adjustments in dose
rate can be made accordingly.
AIM: Determination of total residual chlorine in water.
APPARATUS: Burette, Pipette, Conical flask and Beakers.
CHEMICALS:N/50Na2S2O3, Glacial acetic acid, KI, conc. HCl
THEORY: The estimation of residual Cl2 is based on oxidation of KI by residual chlorine i.e. by
iodometric titration.
When the wafer sample containing residual chlorine is treated with KI, residual chlorine (both
free and combined) oxidizes KI and liberates I2 in equivalent amount.
Cl2 +2KI  2KCl+I 2
OCl- +2KI  2KCl+I 2 +H 2O
HOCl+2KI+HCl  2KCl+I 2 +NH 3
NHCl2 +2KI+2HCl  2KCl+I 2 +NH 3
The liberated I2is titrated against hypo solution, using starch as indicator.
I2  2Na 2S2O3  2NaI+Na 2S4 O6
I2  Starch  Deep blue complex

The end point is disappearance of blue colour.

PROCEDURE:
1. Pipette out 100 ml of the given water sample in conical flask and add 2g of KI solution
and about 3 ml of glacial acetic acid to maintain pH up to 3-4.
2. Cover the flask and shake it well to mix the solution.
3. Using a wash bottle rinse the sides of the flask.
4. Titrate it with N/50 Na2S2O3 solution from the burette till the solution becomes straw
yellow.
5. Add 2ml of the starch solution. The solution will turn blue.
6. Continue titration with Na2S2O3 till the blue colour disappears.
7. Note the final reading and repeat to get concordant readings.

OBSERVATIONS:
Normality of the Na2S2O3solution = N/50
Observation Table
 S. N. Volume of the solution Burette Readings Volume of the
taken in the titration flask titrant used (final-
(ml) Initial Final Reading initial reading) (ml)
Reading
1
2
3
CALCULATIONS:
Sample = Na2S2O3solution
N1V1 = N2V2
1
N1 × 100 =  V2
50
V2
N1 
5000
Total residual Cl2 = N1× Equivalent Weight of Cl2 = N1× 35.5 g L-1= N1× 35.5 × 1000 mg L-1
RESULT: The amount of residual Cl2 in water sample = …….mg L-1.
PRECAUTIONS:
1. Chlorine vapours are harmful so the solution should not be sucked in to the pipette with
mouth.
2. The titration should be completed rapidly in order to avoid atmospheric oxidation of
iodide.
3. First disappearance of blue colour should be taken as end point.
Instruction to the Lab Assistant
Provide the following
1. N/50 Na2S2O3solution: Dissolve 4.96 g of Na2S2O3in distilled water and make up the
volume to 1 litre. Standardize with N/50 K2Cr2O7.
2. Glacial Acetic Acid
3. KI (Solid)
4. Starch solution

EXERCISE
1. Why Cl2 is added to water?
2. What are the disadvantages of excess Cl2 present in water?
3. What is the action of Cl2on pathogens?
 4. Why and how pH is adjusted between 3 and 4?
Ans. pH is adjusted between 3 and 4 using acetic acid. At neutral pH, some combined
chlorine residuals do not liberate I2 by reacting with KI.
EXPERIMENT NO. 7

AIM: Determination of Ammonia in Water.

THEORY: Ammonia is found as ammonium hydroxide in solution and reacts with sulfuric acid
according to the following equation.
H2SO4  2NH4OH  (NH4 )2 SO4  2H2O
REAGENT: Standard solution of H2SO4
METHOD:
1. Rinse and fill the burette with N/50 H2SO4.
2. Pipette out 25mL of the water solution in titration flask.
3. Add 2 drops of methyl red indicator and mix well.
4. Run the acid solution into the flask till the colour changes from yellow to red.
5. Repeat to get three concordant readings.

OBSERVATIONS:
Normality of the acid solution = N/50
Volume of mixture taken for each titration =25mL
Observation Table

S. N. Volume of the water sample Burette Readings Volume of the

taken in the titration flask titrant used (final-
(ml) Initial Final Reading initial reading) (ml)
Reading
1
2
3

CALCULATIONS:
Acid = Water solution
N1V1  N2 V2
1
V1  N 2  25
50
 1 V1
N2  
50 25
Strength = N2×17 g L-1 = N2×17×1000 mg L-1
RESULT: The strength of ammonia in water sample is…….mg L-1.

EXPERIMENT NO. 8
AIM: Determination of free CO2 in given sample of water.
APPARATUS: Burette, Pipette, Conical flask, Beakers etc.
CHEM CALS: N/50 Na2CO3or N/50 NaOH), phenolphthalein
THEORY: Free CO2is determined by titrating the sample water with N/50 NaOH or N/50
Na2CO3 solution using phenolphthalein as indicator.
The CO2 reacts with NaOH or Na2CO3 to form HCO3 .

CO2 + H2O  HCO3

CO2 + CO32- +H2O  2HCO3
The end point is appearance of pink colour.
PROCEDURE:
1. Rinse and fill the burette with N/50 Na2CO3 solution
2. Pipette out 100 ml of water sample in a conical flask (there should be no air bubbles). Add a
few drops of phenolphthalein.
3. Titrate the solution With N/50Na2CO3from the burette, till the pink colour persists for at least
30 seconds.
4. Note the reading and repeat to get three concordant readings.
OBSERVATIONS:
Volume of water sample taken for each titration = 100 ml
Normality of Na2CO3 used = N/50
Observation Table:

S. N. Volume of the water sample Burette Readings Volume of the

taken in the titration flask titrant used (final-
(ml) Initial Final Reading initial reading) (ml)
Reading
1
2
3
CALCULATIONS:
Water sample = Na2CO3
N1V1  N2 V2
1
N1 100=  V2
50
1
N1 =  V2
5000
Strength of CO2 = N1 × Eq. Wt.
V2 V
Strength of CO2 =  22g L1 = Strength of CO 2 = 2 22 1000 mg L1
5000 5000
RESULT: The amount of free CO2 present in water sample is……..ppm.
PRECAUTIONS:
1. The reaction mixture should be stirred very gently during titration.
2. Same amount of indicator should be added while taking different readings.
Instructions to the Lab Assistant
Provide the following
1. N/50 Na2CO3 solution: Dissolve 1.06 g of Na2CO3 in distilled water and make up the
volume to 1 litre.
2. Phenolphthalein.

EXERCISE
1. What is the source of acidity in water?
2. How the acidity of water is determined?
3. What are the disadvantages of acidic water?
4. What are the disadvantages of dissolved CO2 present in water?
5. How can you determine the amount of dissolved CO2 in water?
 EXPERIMENT NO 9

AIM: Determination of pH of some soil samples.

APPARATUS: pH meter or potentiometer, glass electrode, thermometer, beaker, stirrer
THEORY: Soil acts as an electrolyte and hence its pH can be measured. The pH is measured to
determine the degree of acidity or alkalinity in the soil sample. The pH of soil is an important
physico-chemical characteristic because it influences:
• The suitability of a soil for crop production.
• The availability of nutrients in the soil.
• The microbial activity in the soil.
• The lime and the gypsum requirement.
• The soil's physical properties like structure, permeability etc.
For determining the pH of a solution, different methods employed. The most widely used method
is by using pH meter. The value of pH, the logarithm of the reciprocal of the hydrogen ion
concentration in solution, is determined by measuring the difference in potential between two
electrodes immersed in a sample solution.
The method is based on the potential difference between an electrode pair consisting of a glass
electrode sensitive to the difference in the hydrogen ion activity in the sample solution and the
internal filling solution, and a reference electrode, which is-supposed to have a constant potential
independent of the immersing solution. These days, combined electrodes are used in which the
reference electrode is combined with the glass electrode. The measured potential difference is
compared with the potential obtained when both electrodes are immersed in a solution or buffer
with known pH or hydrogen ion concentration. The pH is defined by the formula:

F
pH(sample)  pH(reference)  E (sample)  E (reference)
RTln10
Where, E represent the electrode potentials, R is the universal gas constant, T the absolute
temperature and F is the Faraday constant.
pH interpretation :

S. N. pH Interpretation
1 Below 6.5 Acidic- Requires liming
2 6.5-8.7 Normal- No treatment, optimum for most of the crops
3 Above 8.7 Alkaline-Requires reclamation with gypsum and by growing the salt
toleration crops

PROCEDURE:
1. Soil Sample:
(i) Take the soil sample with the help of clean spatula and crush it if the lumps are
there.
(ii) Take around 50 g of the sample in a beaker and add 100 mL of distilled water.
Stir at least 4 times over a 30 minutes period or continuously shaking for 10 min
on mechanical shaker. This time is allowed for the soil and water to reach
equilibrium.
(iii) Shake the soil suspension and filter it.

2. Calibration: Calibrate the electrode as per the following procedure:

(i) Connect the pH meter to the power supply. Switch on the instrument.
(ii) Take a standard buffer solution of pH 7 in 100 mL beaker. Note the temperature of
the buffer solution.
(iii) Set the temperature control of the pH meter to the temperature of the buffer solution.
(iv) Remove the combination electrode from the storage solution, wash it with distilled
water and blot dry with soft tissue paper.
(v) Connect the combination electrode to the input socket and dip the electrode in 7 pH
buffer solution.
(vi) Set the Function Selector Switch to pH position and adjust with calibrate control till
the digital display shows the pH 7.
(vii) Now move the function switch to ‘Stand by' position.
(viii) Remove the electrode from the buffer solution and wash it with distilled water. Dry
with tissue paper.
(ix) Dip the electrode in the buffer solution having pH 4.
(x) Set the "temperature" to the temperature of the solution.
(xi) Set the Function Selector Switch to pH position and adjust with' Slope correction"
control till the digital display shows the pH 4.

3. pH measurements:
(i) Connect the combination electrode to the input socket after washing it with
distilled water.
(ii) Dip the combined electrode in the soil solution under test.
(iii) Set the Temperature knob to the temperature of the solution.
(iv) Set the Function Selector Switch to pH position.
(v) Note the pH of the solution.
(vi) Repeat the same procedure for other soil samples.

OBSERVATIONS: Soil sample

S.N. Sample pH
1
2
3
4

RESULT: The pH of the given soil samples are……..

PRECAUTIONS:
1. Immediately after testing, the electrodes should be washed off with a gentle stream of
distilled H2O.
2. For storage, after cleaning, the electrodes are suspended in distilled water and the system
is protected against evaporation. The drying out of the electrodes should be avoided.
 EXPERIMENT NO 10
AIM: Synthesis of dibenzalpropanone (Base catalysed Aldol condensation by Green
methodology).
REQUIREMENTS:
Chemicals: Acetone 1ml (0.83 g), benzaldehyde 3.8 ml (3.9 g), NaOH (30 ml) of 10%
solution, Ethanol (15 ml)
Apparatus: Conical flask, Filtration set up.
THEORY: Dibenzalpropanone is prepared by base catalysed Aldol condensation method.

Mechanism of this reaction is given below:

PROCEDURE:

(i) Take 3.8 mL benzaldehyde, 1 mL acetone; 15 mL alcohol in a conical flask. Fit a

cork over the mouth of this conical flask and shake for 2minutes.
(ii) Add 30 mL of 10% sodium hydroxide solution.
(iii) Shake vigorously for 10minutes with simultaneous pressure release.
(iv) Cool the reaction mixture in ice.
(v) Filter the pale yellow solid through filter paper.
(vi) Wash with water, dry, collect, weigh, and recrystallize from ethanol.

RESULT: Yield = 3.0 g (90%)

SIGNIFICANCE:
In this synthesis method non-toxic reagents are used. Hazardous organic solvents are avoided so
it is a green methodology.

5 Innovative Teaching -Learning Methods like

NPTEL(Details of Resources Used)

1. https://nptel.ac.in/courses/103/103/103103026/
2. https://nptel.ac.in/courses/103/105/103105110/
3. https://nptel.ac.in/courses/103/102/103102022/
4. https://nptel.ac.in/courses/103/107/103107156/
5. https://nptel.ac.in/courses/103/106/103106109/

6. Course Objective mapping with Program Outcome

Marking Scheme:
1. Teachers Continuous Evaluation: 40 marks
2. Term end Theory Examinations: 60 marks
Instructions:
1. The course objectives and course outcomes are identical to that of “Applied Chemistry” as
this is the practical component of the corresponding theory paper.
2. The practical list shall be notified by the teacher in the first week of the class
commencement under intimation to the office of the office of the Head of Department /
Institiution in which the paper is being offered from the list of practicals below. Atleast 8
experiments must be performed by the students

Course Outcomes (CO):

CO1 Environmental Studies course will provide necessary information and knowledge about
the various aspects of environment, ecosystems and related biodiversity.
 CO2Students will be able to learn and understand about the availability and sustainable use
of resources, environmental problems and their short term and long term impacts to
humans.
CO3 Course will help them to learn about environmental policies and protocols, social issues
and role of human in conservation and protection of environment.
CO4 Overall, course will help students to develop skills and ability of understanding
environment- human relationship.
Course Outcomes (CO to Programme Outcomes (PO)) Mapping (scale 1: low, 2: Medium, 3:
High)
CO/PO PO01 PO02 PO03 PO04 PO05 PO06 PO07 PO08 PO09 PO10 PO11 PO12
CO1 - 1 1 - - 3 3 2 1 1 1 1
CO2 - 1 1 - - 3 3 2 1 1 1 1
CO3 - 1 1 - - 3 3 2 1 1 1 1
CO4 - 1 1 - - 3 3 2 1 1 1 1

7.Evaluation Scheme
S.No. Marks

1 Internal Assessment 40
(File assessment +Viva+
Innovative Experiment)
1) File assessment (30)
2) Final Viva (5)
3) Innovative (5)

2 External Assessment 60
Total 100

Break up of 15 marks For Laboratory assessment

Attributes Description Score

performance & Execution result Well done with expected result 5
(5)
Error In calculation 3
Incomplete incorrect 0
Viva voice (5) Answer correctly 5
Answer satisfactorily 3
Do not answer any question 0
Record Completion or File (3) Well prepared, write the complete 3
Submission (2) total 5 experiment in the proper manner
Not compile properly 1
Submit In Time(During 2
subsequent lab)
Fail to submit in time 0
 Assessment of COs for year 2021-22
COs mapping Assessment (40 Marks) Internal
(CO1) Experiment 1 &5 (7.5 marks)
(CO2) Experiment 2&6(7.5 marks)
(CO3) Experiment 3& 7(7.5 marks)
(CO4) Experiment 4& 8(7.5 marks)
(CO1-CO4) Case study ,innovation, viva(10
marks)
8. Course End Survey

BHARATI VIDYAPEETH’S COLLEGE OF ENGINEERING, NEW DELHI

Applied Science Department

Course End Survey
Academic Year 2021-2022

Course Name: ENVIRONMENTAL STUDIES LAB Course Code: BS- 162

Program: B.TECH ________ Semester: 2nd

Please rate how well you understood the course (Tick the most appropriate option)

(1 – Poor, 2- Good, 3- Excellent)

Based on your understanding of the course, will you be able to-

BS 162.1 Define and outline the various terms related to water contamination like BOD, COD, TDS,
CO2, NH3, residual chlorine present in water etc..

1. 2. 3.

BS 162.2 Understand and describe the ways in which human activities have more responsible, leading to
problem like water pollution, soil pollution..

1. 2. 3.

ETEN 160.3 Apply the concepts to understand the adverse effects of contamination on human health.
1. 2. 3.

BS 162.4 Evaluate the real world environmental issues and able to assess the possible sustainable
methodology.

1. 2. 3.

Suggestions to improve the practical teaching methodology:

Overall how do you rate your understanding of the subject (tick whichever applicable)

1. Below 50%. 2. 50%-70%. 3.70%-90% 4. Above 90%

Name of student:

Enrolment number: Signature

9 CO attainment
 To be completed after declaration of 2nd Semester final result.

10. Sample evaluated file

Sample copy (All the files available in Chem lab)

11. Attendance Register

All thye register Available in chem lab