Sep Only QP
Sep Only QP
1.
Figure 1 shows how the student set up an agar plate.
Figure 1
The student used aseptic techniques to make sure that only one type of bacterium was growing
on the agar.
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Figure 2
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Use π = 3.14
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(d) Suggest one way the student could improve the investigation.
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(Total 9 marks)
✓) one box.
Tick (✓
Gonorrhoea
HIV
Measles
Salmonella poisoning
(1)
✓) one box.
Tick (✓
(1)
Suggest a different way the man could avoid passing the bacteria on to someone else.
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(1)
A scientist investigated the effect of three different antibiotics on three different types of bacteria,
A, B and C.
2. Put three separate paper discs each containing one of the antibiotics (1, 2 and 3) onto the agar
plate
Figure 1
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(6)
A small volume of raw milk was placed in a counting chamber in a special type of microscope
slide.
Figure 2 shows what the counting chamber looked like when viewed using a microscope.
Figure 2
Table 1
✓) one box.
Tick (✓
Sample E
Sample F
Sample G
Sample H
(1)
(f) Calculate the mean number of bacteria in the four samples in Table 1.
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(g) Calculate the mean number of bacteria per mm3 of milk in the samples.
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Calculate the mean number of bacteria per mm3 of milk in the samples.
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Milk is heated to reduce the number of bacteria it contains before it is sold for humans to drink.
Milk with more than 20 000 bacteria per cm3 cannot be sold for humans to drink.
Table 2 shows the number of bacteria per cm3 in four different samples of milk.
Table 2
✓) one box.
Tick (✓
P Q R S
(1)
(i) Why should milk sold for humans to drink not contain large numbers of bacteria?
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(Total 17 marks)
Scientists have found the following food web in the cold Antarctic Ocean.
3.
Some scientists argue that this could lead to a decrease in the numbers of squid and
penguins.
Others argue that the numbers of squid and penguins will stay the same.
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(2)
(c) Explain, as fully as you can, why the conversion of shrimp biomass into cod biomass is
more efficient than that of cod biomass into seal biomass in the cold Antarctic Ocean.
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(3)
(d) Boats from many countries fish the Antarctic Ocean. The cod are being overfished. If the
numbers of cod are to increase, the population must be carefully managed.
(i) Suggest two control measures which would prevent a further drop in numbers,
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(Total 11 marks)
The diagram shows a fermenter. This fermenter is used for growing the fungus Fusarium which is
4. used to make mycoprotein.
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(c) The fermenter is prevented from overheating by the cold water flowing in through the heat
exchanger coils at C.
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(d) It is important to prevent microorganisms other than Fusarium from growing in the
fermenter.
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(ii) Suggest two ways in which contamination of the fermenter by microorganisms could
be prevented.
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(2)
The table shows the amounts of four of these amino acids present in mycoprotein, in beef
and in wheat.
A diet book states that mycoprotein is the best source of amino acids for the human diet.
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(4)
(Total 11 marks)
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(d) It is important to prevent microorganisms other than Fusarium growing in the fermenter.
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(ii) Suggest one way in which contamination of the fermenter by microorganisms could
be prevented.
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(e) Human cells cannot make some of the amino acids which we need. We must obtain these
amino acids from our diet.
The table shows the amounts of four of these amino acids present in mycoprotein, in beef
and in wheat.
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(4)
(Total 10 marks)
Figure 1
(a) The animals in Figure 1 get their energy by eating other organisms.
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Complete Figure 2 to show a food chain in Figure 1 with five organisms, including the
human.
Figure 2
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The biomass of mackerel is much less than the combined biomass of krill and squid.
One reason for this is that the mackerel cannot digest all parts of the krill and squid.
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Figure 3
(f) Calculate the percentage decrease in the biomass of herring between 1960 and 1977.
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Herring can live for up to 12 years and begin to reproduce when 3 to 4 years old.
Figure 4
Figure 3
Use data from Figure 3 and information from Figure 4 in your answer.
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(6)
(Total 17 marks)
Recently scientists have produced mAbs to Candida albicans using human lymphocytes
produced naturally after an infection.
A sample is taken from the throat of a patient with a suspected Candida albicans infection.
Describe how the mAbs and a fluorescent dye could be used to see any Candida albicans
pathogens on the slide.
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(3)
In a laboratory the human lymphocyte mAbs were injected into animals infected with Candida
albicans.
Doctors intend to start a trial to give the mAbs to patients severely ill with Candida albicans.
(b) Explain how increased phagocytosis of the Candida albicans pathogen will help the patient.
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(2)
The mAbs treatment for Candida albicans is now ready for clinical trials on people.
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(6)
(d) Scientists have also used human lymphocytes to make mAbs to other pathogens and to
some types of cancer cells.
Suggest one reason why these new mAbs have been more successful in treating diseases
in humans than mAbs made using mice.
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(Total 12 marks)
What is an enzyme?
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(2)
Name two other organs in the digestive system that produce carbohydrase.
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(2)
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(4)
Some people have a gene mutation that stops cancer cells producing enzyme B.
The following figure shows how cell division is controlled in pancreatic cancer cells.
The drug is given to pancreatic cancer patients who have the gene mutation that stops cancer
cells producing enzyme B.
(d) Explain why the drug can be used to treat pancreatic cancer in patients with the gene
mutation.
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(3)
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(f) The drug was trialled before it was licensed for use.
A placebo __________________________________________________________
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(g) One stage in a drug trial is to test the drug on healthy volunteers.
✓) one box.
Tick (✓
(1)
St Clement Danes School Page 26 of 41
(h) A monoclonal antibody has been produced to treat pancreatic cancer.
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(3)
(Total 19 marks)
Monoclonal antibodies are used to measure the levels of hormones in the blood.
9.
Pregnant women produce the hormone HCG.
Figure 1
A B C D
(1)
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Figure 2
The pregnancy test strip will show a positive test result when a woman is pregnant.
Explain how the pregnancy test strip works to show a positive result.
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(6)
(Total 8 marks)
(a) An aphid feeds by inserting its sharp mouthpiece into the stem of a plant.
Give the reason why the mouthpiece of an aphid contains a high concentration of dissolved
sugars after feeding.
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(5)
To make the monoclonal antibodies a scientist first isolates the PVY protein from the virus.
Describe how the scientist would use the protein to produce the PVY monoclonal antibody.
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(4)
(Total 10 marks)
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(3)
(c) Describe how injecting a monoclonal antibody for RSV helps to treat a patient suffering with
the disease.
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(2)
A trial was carried out to assess the effectiveness of using monoclonal antibodies to treat
patients with RSV.
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(e) Calculate the total number of patients admitted to hospital with RSV during the trial.
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(f) Evaluate how well the data in the table above supports the conclusion:
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(2)
(Total 12 marks)
✓) one box.
Tick (✓
(1)
(b) Name the sub-cellular structures where aerobic respiration takes place.
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(d) Describe two differences between aerobic and anaerobic respiration in humans.
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(2)
✓) two boxes.
Tick (✓
Carbon dioxide
Ethanol
Glucose
Lactic acid
Water
(2)
Figure 1
After 5 days, the scientist completely covered the boiling tube with black paper.
Figure 2 shows the concentration of carbon dioxide inside the boiling tube over 15 days.
Figure 2
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(2)
(g) Suggest why the concentration of carbon dioxide increased between day 5 and day 10.
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(1)
Explain why the death of the pond snail caused the concentration of carbon dioxide to
increase after day 10.
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(3)
(Total 14 marks)
Type of response
(2)
Figure 1
(b) Hornets are insects that sting other animals and cause pain.
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Figure 2
Explain why a tree might die if the roots of the tree are damaged.
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(6)
(d) The larvae of the hornet moth form when fertilised eggs divide by mitosis.
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(4)
Name the process by which these stem cells then form specialised cells.
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(Total 14 marks)