PH Le Botomy
PH Le Botomy
PORTFOLIO
TITLE: PAGE #:
Introduction............................................................................…..............3
Unit 01 [Introduction to
phlebotomy]......................................................................................…4-8
Unit 02 [Medical
Terminology].........................................................................................9-18
Physiology].............................................................................................19-21
Importance].........................................................…...…………………22-33
Unit 05 [Phlebotomy
Techniques].........................................................................................….34-38
Procedures].................................................................................……..39-42
Relationship]...........................................................…….……….……43-45
….....46-50
1
Unit 09 [Regulatory Guidelines for Phlebotomy
Practice]................................................................……………..51-53
…………..........................54-56
Equipment].......................................................................…….57-58
References…………………………………………………….62-64
2
INTRODUCTION
Phlebotomy is defined as the surgical opening or puncture of a vein in order
to withdraw blood. This portfolio is consist of 12 units, each unit will give you a full
expand the understanding of those you wants to know more about phlebotomy. I
completed this portfolio by doing researches on the internet, in books and my own
knowledge, upon completion of this portfolio I’ve faced challenges such as limited
3
INTRODUCTION
TO
PHLEBOTOMY
[UNIT 01]
4
I am a visual learner, I mostly learn this faster the more I see what is happening. As I
go further in this course I would like to develop kenetic and auditory learning. Going
forward in this course I would love to develop a full understanding of phlebotomy and
multitasking skills. I will achive these skills by using visual learning style. I plan on
leaning when the teacher is dictating I would have to listen more to write what is
being said and that will also help me to develop my writing skills whereas the teacher
will be reading fast therefore I would have to write faster. Kenetic learning will be
as soon as I start doing the practical part of the course; because I will start using my
hands more. I will achieve the skills by the help of my teacher, classmates, and by
doing the practical part of the course. My teacher will help me to develop my
or quiz together whereas I would have to learn to communicate with others. By doing
this practical part course I think it is the most important part to help me develop most
of the skills I would like achieve it will help me to achieve skills such as multitasking,
writing,hand eye coordination, and communication skills. By doing the practical part
of the course this will firstly help with communication, whereas I would have to
have to indroduce myself. explain the procedure to convince them. it will help with
the writng skills by having to write quickly on tubes. Hand eye coordination will be
developed by seeing the vein and being able to perform venepuncture. Multitasking
will be achieved by comfort and reassuring the patient whilst doing the procedure.
5
The skills developed can benefit the patient by them feeling better and safer when
doing the procedure and help them to get through faster. For example if my
communication and multitasking skills are developed while doing the doing the
procedure I can speak to them more and assure them that it will not be a very painful
procedure and there is nothing to worry about. Multitasking skills may also help by
saving time, while in the vein I could invert the tubes which will be a shorter wait for
the patient. And also if my writing are achieved I can write up the tubes faster so they
can leave quickly, because the tubes have to be wriiten up in there presence.
6
Cave District;
Cave P.O;
Westmoreland
Jamaica;
Dear Kimberly;
As we were speaking on the phone last night and you told me you would like to apply
for the position of a phlebotomist; I want to take the time out to congratulate you and
members have their role and responsibilities to carry out. I have been working as a
Phlebotomist for two (2) years now and it has been a good experience, sometimes
there are complications that I face as the patients not cooperating but by portraying
compassion and professional ethics I handled the situation very well. On a daily basis
about 150 patients are being catered for. As a Phlebotomists I play a vital role, my
responsibility is to collect blood for donation or for testing, so the blood can be
analyzed in the laboratory which is then used for blood test to diagnose illness,
7
evaluate the effectiveness of medications and determine whether a patient is receiving
confidentiality. You must be able to multitask and communicate very well, you must
also always portray professional ethics and be very compassionate when dealing with
the patients. Supervision and support are an important part of Phlebotomy because
Phlebotomy Supervisors ensure that safety rules and regulations are met, and that the
laboratory is orderly, ensures duties are carried out correctly and well stocked for
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MEDICAL
TERMINOLOGY
[UNIT 02]
9
CLINICAL LABORATORY
the human body (such as fluids, tissues, or cells) for the purpose of providing
blood, study DNA, examine tissue, or study cells. He/She may be a research
Two test that are performed in the clinical laboratory are cytology and
immunohistochemistry.
Blood and Urine are two specimens that are usually sent to the Clinical Laboratory
Department.
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HEMATOLOGY LABORATORY
In the hematology laboratory, manual and automated test techniques are used in the
study of blood
and other body cells and fluid. Blood tests obtained in the laboratory are used by the
clinician for
condition.
Full Blood Count Testing and Hemoglobin Testing are two Laboratory test done in
Blood
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CYTOLOGY LABORATORY
The Cytology laboratory specimens from various body sites for the detection of
cancer,
Two tests that are performed in the cytology laboratory department are Breast
cytology and Thyroid cytology.
Specimens that are sent to the Cytology Laboratory are Spit and Mucus (also called
phlegm or sputum)
12
MICROBIOLOGY LABORATORY
Two Laboratory Tests that are performed in the Microbiology Laboratory are
Bacteriology Tests and
Mycobacteriology Test.
Specimens that are sent to the Microbiology laboratory department are Blood and
Sputum.
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IMMUNOLOGY LABORATORY
These are proteins made by a type of white blood cell in response to a foreign
Two test that are performed in the Immunology Laboratory Department are
Specimens that are sent the Immunology Laboratory department are peritoneal fluid,
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DEPARTMENTS IN THE HOSPITAL SETTING, DESCRIPTION OF WHAT IS
Oncology: Oncology is the field of medicine that deal with the diagnosis,
MEDICAL PERSONNEL:
Oncologist
the male and female urinary tract and the male reproductive organs.
MEDICAL PERSONNEL:
Urologist
MEDICAL PERSONNEL:
Proctologist
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DEPARTMENT AND DESCRIPTION:
Cardiology: Cardiologists diagnose, assess and treat patients with defects and
diseases of the heart and the blood vessels, which are known as the
cardiovascular system.
MEDICAL PERSONNEL:
Cardiologist
and treatment of hormones as well as the glands that produce them. This includes
MEDICAL PERSONNEL:
Endocrinologist
brain and spinal cord, and disorders of the nerves and muscles that activate
movement and transmit sensations from around the body to the brain.
MEDICAL PERSONNEL:
Neurologist
16
DEPARTMENT AND DESCRIPTION:
MEDICAL PERSONNEL:
Anesthesiologist
MEDICAL PERSONNEL:
Orthpedists
MEDICAL PERSONNEL:
Ophthalmologists
17
DEPARTMENT AND DESCRIPTION:
providers who specialize in diseases of the blood and blood components. These
MEDICAL PERSONNEL:
Hematologist
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ANATOMY
AND
PHYSIOLOGY
[UNIT 03]
19
BODY MAIN DISORDER TEST COLOU LAB
SYSTEM FUNCTION R TOP DEPT
TUBE
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body its
shape and
provides
protection
for organs.
our body hy
tissues by
removing all
fluids that
leak out of
our blood
vessels.
21
THE STEPS IN
PHLEBOTOMY
PROCEDURE AND
THEIR IMPORTANCE
[UNIT 04]
22
Step 1. Assemble equipment
Collect all the equipment needed for the procedure and place it within safe and easy
reach on a tray or trolley, ensuring that all the items are clearly visible. The equipment
required includes:
A supply of laboratory sample tubes, which should be stored dry and upright in a
well-fitting, gloves.
a tourniquet.
writing equipment.
laboratory forms.
23
leak-proof transportation bags and containers.
24
Step 2. Put on laboratory coat then perform hand hygiene and put on glove.
Wash hands with soap and water, and dry with single-use towel
if hands are not visibly contaminated, clean with alcohol rub use 3 ml of
alcohol rub on the palm of the hand, and rub it into fingertips, back of hands
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Step 3. Identify and prepare the patient
Introduce yourself to the patient, and ask the patient to state their full name.
Check that the laboratory form matches the patient's identity (i.e. match the
Ask whether the patent has allergies, phobias or has ever fainted during previous
If the patient is anxious or afraid, reassure the person by telling them it will not be
very painful and it only takes a minute to perform the venipuncture procedure and
Discuss the test to be perform and obtain verbal consent. The patient has a right
to refuse a test at any time before the blood sampling, so it is important to ensure
26
Step 4. Select the site
General
Extend the patient's arm and inspect the antecubital fossa or forearm.
Locate a vein of a good size that is visible, straight and clear. The median cubital
The vein should be visible without applying the tourniquet. {If the vein is not
visible apply tourniquet, tell the patient to make a fist and palpate to locate the
vein. Once vein is being located loose the tourniquet}. Locating the vein will help
27
Step 5. Disinfect the entry site
Clean site using 70% isopropyl alcohol, Apply firm but gentle pressure. Start
from the centre of the venipuncture site and work downward and outwards to
Allow the area to dry. Failure to allow enough contact time increases the risk of
contamination.
NB: DO NOT touch the cleaned site; in particular, DO NOT place a finger over
the vein to guide the shaft of the exposed needle. It the site is touched, repeat the
disinfection.
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Step 6. Take blood
Venipuncture
Apply tourniquet
Ask the patient to form a fist so the veins are more prominent.
pull skin back and enter the vein swiftly at a 30 degree angle or less, and continue
to introduce the needle along the vein at the easiest angle of entry.
Draw blood collection tubes in the correct order, to avoid cross contamination
Release the tourniquet as soon as blood enters the last tube upon collecting the
Withdraw the needle gently and apply gentle pressure to the site with a clean
gauze or dry cotton-wool ball. Ask the patient to hold the gauze or cotton wool in
place, with the arm extended and raised. Ask the patient NOT to bend the arm,
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Step 7. Labeling\writing up of tubes :
While the patient is pressing\holding down on the cotton\gauze write up the tubes
30
Step 8. Apply bandage:
After writing up the tubes, take cotton\gauze off the area the venipuncture was
performed{if excess cotton is lft on the site take another cotton to clean area then
31
Step 10: Remove Lab coat then Gloves
Removing Gloves
32
TWO STEPS IN THE PHLEBOTOMY PROCEDURE AND THE
IMPORORTANCE OF EACH:
patient can have serious, even fatal, consequences, especially specimens for type
pathogens.Failure to clean site before doing the venipuncture procedure this can
allow bacteria to infect the puncture site. A serious infection of the blood
33
PHLEBOTOMY
TECHNIQUES
[UNIT 05]
34
FOUR DIFFERENT PHLEBOTOMY TECHNIQUES:
Four different ways of taking blood were observed: evacuated system, butterfly with
Four different ways of taking blood were observed: cannulation and a syringe (38%),
cannula with evacuated tube and adaptor (42%), syringe and needle into vein (14%)
Evacuated System:
Double-pointed needle
the syringe
Needle
35
Butterfly with vacutainer:
Butterfly needles enable IV access using a small needle for IV infusions or blood
draws.
Using a butterfly needle reduces the likelihood a person will experience profuse
36
Allows for greater flexibility during angled insertion, accuracy and is typically
Syringe system:
. They are less likely to collapse under a vacuum which is controlled by the
technician.
This method requires less manipulation than the Vacutainer System for small
volume draws (1-2 tubes).
If the needle is not in the vein and the phlebotomist attaches the tube, no blood
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Because of their small needle size, blood collection tends to be slower
Takes more time: A smaller needle draws blood at a slower rate than a larger one.
May cause hemolysis: Needles with a gauge size 25 or higher are not always
increases.
Syringe system:
There is the potential for needle stick when depositing blood into the collection
tube.
Excessive resistance may lead to the build-up of pressure and eventual failure of
The flow rate during the transient period cannot be known without flow sensors.
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CLINICAL
LABORATORY
PROCEDURES
[UNIT 06]
39
DESCRIPTION OF EACH SPECIAL COLLECTION PROCEDURES:
Bleeding time tests: Bleeding time is a medical test that measures how fast small
blood vessels in the skin stop bleeding. The bleeding time test is used to evaluate
how well a person's blood is clotting. The test evaluates how long it takes the
vessels cut to constrict and how long it takes for platelets in the blood to seal off
the hole.
Arterial Blood Gases: An arterial blood gases (ABG) test is a blood test.
It measures the acid-base balance (pH) and the levels of oxygen and carbon
dioxide in the blood. It uses blood drawn from an artery. This is where the levels
of oxygen and carbon dioxide can be measured before they enter body tissues.
Cold Agglutinin Test: A cold agglutinins blood test is done to check for
conditions that cause the body to make certain types of antibodies called cold
response to infection. They cause red blood cells to clump together (agglutinate)
at low temperatures.
is a specific test that determines the amount of FDPs in your blood. The test is
also known as the fibrin split products (FSPs) test, or the fibrin breakdown
products test.
40
COMPARISON BETWEEN ROUTINE VENEPUNCTURE SITE PREPARATION
The most critical step in collecting a blood culture is the proper cleansing of the site.
Bacteria are normally present on the skin surface. It is imperative for quality test
results that bacteria is not introduced into the specimen being collected. Before the
collection of a specimen for blood culture analysis, the selected venipuncture site
be used on the site. Blood culture collections require a sterile site, one that is free of
living microorganism. Due to the special site preparation once the site has been
prepared do not repalpate, Repalpating the site will contaminate it. The traditional
method involves first cleansing the site for 60 seconds with alcohol to remove the oils
and dirt on the skin surface. The site is then painted with a 2 percent tincture of 70
percent alcohol which is allowed to dry to killsurface skin bacteria. .the only recourse
is to cleanse thoroughly with 70 percent alcohol is painted on the area, with a circular
motion, starting at the site of the puncture and moving in concentric circles outward.
Do not flood the site.The cleansing procedure varies from one laboratory to another.
Instead of using a syringe to draw the blood and inoculate the bottles, a butterfly
collection set can be used. An adapter can be attached to the end of the butterfly
which attaches to the blood culture bottle and directly inoculates the bottle with blood.
Each bottle is filled to the proper level of blood. There are also blood culture bottles
with long necks that insert into an evacuated needle holder. These are the preferred
methods because they minimize the chance of contamination due to the blood being
drawn directly into the prepared tube. It is critical that the tops of the tubes be
cleansed prior to collection.Blood cultures are always collected first. The blood is
41
collected and placed in a bottle containing a solution that enhances the growth of
42
COMMUNICATION
AND
INTERPERSONAL
RELATIONSHIPS
[UNIT 07]
43
AN EFECTIVE COMMUNICATION BETWEEN A PHLEBOTOMIST AND A
VOICE.
A youtube video was observed, the positioning and proximitiy, patient was sitting on
a chair with a upright posture being maintained during the entire venepuncture
procedure. The phlebotomist portray excellent use of eye contact with the patient,
while the identification process the phlebtomist was using eye contact efficiently. The
phlebotomist kept a pleasant facial expression throughout the entire procedure, the
patient’s facial expression was also pleasant which looked pleased with what the
phlebotomist was doing. The phlebotomist did a positive gesture(thumbs up), she did
this when compended her for doing a good job, she notifys the patient before touching
her hand to perform venepuncture which is good because it would be offensive if she
touched the patient without her consent or the patient may get a fright especially if
she’s afraid of the procedure. The phlebotomist also used language effectively. The
Phlebotomist spoke to the patient with respect and the words she used was not
offensive, the plebotomist tone of voice was moderatly good. There is room for
improvement for the phlebotomist tine of voice is was a bit low where the patient ask
her to repeat once. From the video I have learnt that skills needed to communicate
effectively are you have to be an active listener, so if the patient ask you a question
you can answer there questions effectively, also you must be clear when speaking to
the patient meaning telling them the exact thing. Upon communicating with the
patient you must also be relaxed as much as possible, reason being if you are acting
44
panicked the patient will think you do not know what you are doing and they would
start to get worried and be panicked also or if you are not relaxed when answering a
question they asked are if you explaning the procedure they may not understand what
comunicating, when you inform the patient what you are doing it eliminates the fear
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PROFSSIONAL
ETHICS
[UNIT 08]
46
THREE (3) PROFESSIONAL ETHICS A PHLEBOTOMIST SHOULD PROTRAY:
includes adherence to the scope and standards of medical practice and such moral
This is a image showing that the phlebotomist is portraying respect and dignity to a
visually-impaired patient by shaking her hand and treating her with compassion and
care. The patient was given the chance to have their views listened to by the
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This image is showing self determination; this is where the patient decide whether or
This image is depicting that the phlebotomist is showing gratitude; a positive emotion
that involves being thankful and appreciative to the patient. For example thanking
him/her for their time and patience and for being cooperative etc.
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THREE (3) ATTRIBUTES A PHLEBOTOMIST SHOULD PORTRAY:
Hand–eye coordination
the way that one's hands and sight work together to be able to do things that require
speed and accuracy. For example as a phlebotomist when your eyes sees the vein you
In this image the phlebotomist is portraying hand-eye coordination. She saw the vein
Ability to Multitask
Phlebotomists work in busy environments and have to handle several specimens, meet
tight deadlines, therefore having the skill to do more than one things at once
[multitask] is essential.
49
The image showing that the phlebotomist is multitasking. She is doing the
Team Player
Medical staff is essentially a big team, it's important for a phlebotomist to be able to
work well with other people in order to deliver the highest standard of care possible to
patients.
The image above is showing three (3) phlebotomist communicating with each other
about how the can improve the patients needs. Each phlebotomist contributes by
adding there input on how they think they can be a better worker.
50
REGULATORY
GUIDELINES FOR
PHLEBOTOMY
PRACTICE
[UNIT 09]
51
ONE (1) REASON WHY IT IS IMPORTANT FOR THE PHLEBOTOMIST TO
serious injury, for example if OSHA states that you should not recap the needles
and you disobeyed, recapping can cause injury by sticking your self and it can
(HIV).
Standard of Care: Standard of Care is the level of attentiveness and care that a
reasonable treatment option available and the phlebotomist does not pursue it,
and the patient suffers as a result, then he may be subject to a lawsuit for
Tort: A wrongful act, other than breach of contract, committed against one's
Plaintiff: A Plaintiff is person who brings a case against another in a court of law
or the party who brings a legal action or in whose name it is brought as opposed
52
Defendant: A person or group against whom a criminal or civil action is
Intentional Torts: The act of intentionally causing someone to fear that he or she
leads to injury, property damage, or financial loss. The person who caused the
accident is considered negligent because they failed to exercise the same degree
53
WASTE DISPOSAL
AND
DECONTAMINATION
TECHNIQUES
[UNIT 10]
54
Waste Disposal: Waste Disposal is any solid waste that is generated in the diagnosis,
special bags and plastic boxes in clinical settings and then disposed of like normal
trash.
DISPOSED
container.
toilet.
environment.
Program.
bags.
55
FEATURES AND USE OF
PHLEBOTOMY
EQUIPMENT
[UNIT 11]
56
FLIER ADVERTISING THE DIFFERENT PHLEBOTOMY EQUIPMENT MY
57
QUALITY
ASSURANCE
[UNIT 12]
58
Mr. Barry Green’s sample was collected by a phlebotomist working in a health centre.
The requisition form came back stating the sample was unsuitable for testing.
because errors resulting from a failure in this step can, at best, provide results of
iii. Contamination from infusion route: Blood collection above an IV catheter leads
to IV contamination of blood samples, which can cause inaccurate lab test results.
samples because all results are unreliable. Clots are not always detected and can
cause falsely low or high results. If the amount of blood taken is too small then
59
An individual you are collecting blood from starts to develop a develop a Syncope.
Syncope: Syncope is another word for fainting or passing out. Someone is considered
to have syncope if they become unconscious and go limp, then soon recover.
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INCIDENT REPORT FORM
EMPLOYEE DETAIL
NAME: Phebe T. Mahabier
DEPARTMENT: Phlebotomy
PHONE NUMBER: 1876-869-6368
ACTIONS TAKEN: Discontinue procedure, apply gauze, and put the patients head
between his knees.
SIGNITURE: P. Mahabier
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