Mark Scheme (Results)

October 2022

Pearson Edexcel International Advanced Level In Biology (WBI13)

Paper 01 Practical Skills in Biology I
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October 2022
Publications Code WBI13_01_2210_MS
All the material in this publication is copyright
© Pearson Education Ltd 2022
General Marking Guidance

• All candidates must receive the same treatment. Examiners must mark the first candidate in exactly the
same way as they mark the last.
• Mark schemes should be applied positively. Candidates must be rewarded for what they have shown they
can do rather than penalised for omissions.
• Examiners should mark according to the mark scheme not according to their perception of where the grade
boundaries may lie.
• There is no ceiling on achievement. All marks on the mark scheme should be used appropriately.
• All the marks on the mark scheme are designed to be awarded. Examiners should always award full marks
if deserved, i.e. if the answer matches the mark scheme. Examiners should also be prepared to award zero
marks if the candidate’s response is not worthy of credit according to the mark scheme.
• Where some judgement is required, mark schemes will provide the principles by which marks will be
awarded and exemplification may be limited.
• When examiners are in doubt regarding the application of the mark scheme to a candidate’s response, the
team leader must be consulted.
• Crossed out work should be marked UNLESS the candidate has replaced it with an alternative response.
Question Answer Additional Guidance Mark
Number
1(a) An explanation that includes the following
points:

• because (antioxidants / vitamin C) Accept neutralise, get rid of,

reduce (the quantity of) free inhibit, plus many other ways of
radicals (1) saying this

• that cause damage to {cells / tissues

/ blood vessels / endothelium} (1) Expert
(3)
• reducing {plaque / atheroma}
formation (1) Ignore ref to clots
Question Answer Additional Guidance Mark
Number
1(b)(i) A description that includes the following
points:

• use {equal / controlled} masses of

the foods (1)

• use {standard / same} extraction Accept stated detail e.g. volume of

method (1) distilled water / time of macerating
/ standard filtering method

• measure out {equal / same} volume measure out {equal / same} food
of DCPIP solution (1) extract

• add food extract dropwise until {no Add DCPIP solution dropwise until
colour remains / blue to colourless} stays blue / colour stays / no
(1) change

• record {volume / number of drops}

used (1)

• explanation of how to calculate e.g. conc. vitamin C = vol. of

vitamin C content / description of standard Soln. vit C ÷ volume of
calibration of DCPIP with known food extract added
vitamin C concentration (1)
Ignore colorimeter, colour charts
Expert
(5)
Question Answer Additional Guidance Mark
Number
1(b)(ii) • types of {food / fruit} ALLOW (storage) time / (storage) Grad
temperature (1)

Question Answer Additional Guidance Mark

Number
1(c)(i) An answer that includes the following correct answer with no working gains
steps: both marks

• concentration at start –
concentration after 3 weeks (1) e.g. 4.4 – 1.6 = 2.8

Expert
• correct answer to mp1 divided by 3 (2)
given to 2 significant figures (1) e.g. 2.8 ÷ 3 = 0.93, accept .93
ignore sign, 0.93333 would get 1
mark
Question Answer Additional Guidance Mark
Number
1(c)(ii) An answer that includes the following
points:

• increase in storage time decreases Piece together

the vitamin C concentration (in all
juices at both temperatures) (1)

• vitamin C concentration is less at vitamin C concentration more affected

4°C (than 20°C) in orange and lime at 4°C (than 20°C) in orange and lime
(1)
vitamin C concentration less affected at
• vitamin C concentration is more at 4°C (than 20°C) in (carrot and)
4°C (than 20°C) in (carrot and) pineapple (1)
pineapple (1)

• very little difference in effect at 4°C Expert

and 20°C in carrot (1) (4)

• no {range bars / standard deviation}

so cannot tell if differences are
significant (1)
Question Answer Additional Guidance Mark
Number
2(a)(i) • peptide (bond) Grad
(1)

Question Answer Additional Guidance Mark

Number
2(a)(ii) • hydrolysis Grad
(1)

Question Answer Additional Guidance Mark

Number

2(b)(i) • 0.018
Expert
(1)
Question Answer Additional Guidance Mark
Number
2(b)(ii) A graph showing the following initial rate 0.02
features: of reaction
/ au min-1
0.016
• A axes correct (x –
substrate concentration /
0.012
y – initial rate of reaction
/ (1)
0.008

• L axes correctly labelled

and with units (1) 0.004

• S suitable scale on the x- 0

axis (1) 0 1 2 3 4
substrate concentration (%)
• P correct plotting on a
linear scale on y (1)

Ignore line Expert

Allow percentage concentration for y (4)
Allow ecf if miss mp1 for mp3 and 4
Allow use of standard form on y axis
Allow ecf from 2bi in the plot including if 2bi blank
Question Answer Additional Guidance Mark
Number

2(b)(iii) • 1.8
Clerical (1)

Question Answer Additional Guidance Mark

Number

2(b)(iv) An explanation that includes the following

points:

• the rate increases (linearly) as the Accept positive correlation

substrate concentration increases (1)

• because there (is more substrate so)

{more collisions / more ES}
complexes (1)

• does not level off because the Reverse argument if their graph less
{enzyme concentration does not steep or plateau after 2%
become limiting / enzyme’s active
sites are not all occupied at the expert (3)
highest substrate concentration} (1)
If graph goes down at 3% ignore
Question Answer Additional Guidance Mark
Number

2(c)(i) A table drawn with the following features: enzyme enzyme

activity in activity in
• suitable table drawn (1) pH stem / a.u. fruit / a.u.
4.(0) 1.8 0.7
• headings with units (1) 7.(0) 5.1 2.3
8.5 6.6 2.5
• all data correctly entered (1)
10.(0) 1.9 1.5 expert (3)

Allow ±0.1 for each cell

Question Answer Additional Guidance Mark

Number

2(c)(ii) An answer that includes the following points:

• stem (extract) because {more active / Accept reverse

higher} (than fruit extract) (1)

• at all pH values (1) Accept ecf if say fruit for mp 1

• manipulation of data to support mp 1 e.g. stem {4.1 au / 2.64 times} more active
(1) at pH 8.5 / comparison of a pair or more of expert (3)
values
Accept ecf if say fruit for mp 1
Question Answer Additional Guidance Mark
Number

3(a)(i) An answer that includes the following

points:

• description of the use of all three No mark if growing in soil

solutions to grow onions (1)
e.g. age, variety (ignore species) NOT size
• description of a relevant biotic as it is DV
factor kept constant (1)
e.g., light/ temperature / pH of solution
• description of a relevant abiotic
factor kept constant (1)

e.g. height, length, mass

• appropriate measurement of {plant
parts / plant} made at start and Accept if it is clear all started at same
finish (1) height/mass etc. / if measure difference
or increase in height / mass etc. expert (4)

• after {same/ specified / stated} if time quoted must be a week minimum

time (1)
Question Answer Additional Guidance Mark
Number

3(a)(ii) An answer that includes the following

steps:

• correct values from graph correctly e.g. 5g for shoot + bulb, 6.2 g for total
manipulated (1)

• correct percentage (1) e.g. 80.6 (%)

correct with no working gains both marks

allow even if not in table
if no working allow correct answer which expert (2)
is incorrectly rounded for 1 mark (80.64 or
81)

Question Answer Additional Guidance Mark

Number

3(a)(iii) An answer that includes the following

points:

• as solution concentration goes up Accept higher / est (for all parts) in ½

growth (of all parts) goes down (1) strength
Accept lower / est (for all parts) in 2x
strength
Accept reverse argument

• the effect of solution concentration Allow description of relative effects, e.g.

on mass is {greatest in shoots / least percentage increase in bulbs greatest
in roots} (1)

• percentage edible is unaffected / ALLOW double strength solution reduces expert (3)
little affected by solution edible percentage or reverse
concentration (1)
Allow ecf if aii wrong
Question Answer Additional Guidance Mark
Number

3(a)(iv) A description that includes three of the

following points:

• grow several plants in each solution Accept repeat experiment

(1)

• calculation of (means and) SDs (1)

• check for overlap (of means plus and ALLOW accept ranges, error bars in place
minus SD) for each difference (1) of SD
expert (3)
• carry out a statistical test (1) ALLOW t test, ignore Chi, correlation

Question Answer Additional Guidance Mark

Number

3(b) An answer that includes the following

points:
• remove potassium hydrogen Accept don’t add KH2PO4 / use solution
phosphate (1) without KH2PO4
Ignore remove phosphate ions
• replace potassium removed / add
extra of named salt to adjust Accept idea that as there is KNO3 there is Expert (2)
balance (1) no need to do anything but remove KH2PO4
Question Answer Additional Guidance Mark
Number
3(c) An answer that includes the following
points:

• because this is the highest (uptake)

of all mineral ions

• {below 6.5 / at 8.0-8.5} phosphate in

short supply / {above 8.0 / below
5.5} nitrate in short supply (1)
e.g ATP, DNA, RNA, nucleotides,
• needed for appropriate {substance cell division, energy exchange,
/process} (1) protein synthesis.
Allow ecf from mp2
expert
(4)
• pH {above / below} this range will
denature enzymes (1)
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