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HPLC Calibration Format

This document provides a calibration certificate for an HPLC instrument. It details acceptance criteria and observations for calibrating the instrument's flow rate, injection volume accuracy, injector linearity, auto sampler carryover, column heater temperature, sample tray chiller temperature, and detectors. The calibration was performed and complies with the criteria.

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ashishchandan13
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247 views

HPLC Calibration Format

This document provides a calibration certificate for an HPLC instrument. It details acceptance criteria and observations for calibrating the instrument's flow rate, injection volume accuracy, injector linearity, auto sampler carryover, column heater temperature, sample tray chiller temperature, and detectors. The calibration was performed and complies with the criteria.

Uploaded by

ashishchandan13
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOC, PDF, TXT or read online on Scribd
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Page Page 1 of 2

Format No.: QC047/F02 Version No.: 00 Effective Date:

HPLC CALIBRATION CERTIFICATE


Instrument Name
Instrument Model No.
Instrument ID
Analyst
Date of Calibration
Next Calibration due date

S. Description of the
Acceptance Criteria Observation Remarks
No. calibration parameter
Calibration of flow rate
1.
Port A B C D ---
0.49 mL/min to 0.51
0.5 mL/ min
mL/min.
0.98 mL/min to 1.02
1.0 mL/min
mL/min.
1.96 mL/min to 2.04
2.0 mL/min
mL/min.
Calibration of GPV
2.
A/B/C A/B/D ---
The relative height of
19.0 to 21.0 %
peak
The measure value
Injection volume should be between ± 1
3.
accuracy µl of the injection
volume 50 µl.
4. Injector linearity
Injection volume (µL) 5 10 20 50 100 ---
%RSD of area NMT 1.0
%RSD of RT NMT 0.5
Correlation
coefficient NLT 0.999

S. Description of the Acceptance Criteria Observation Remarks

Format No. QA001/F02


Page Page 2 of 2

Format No.: QC047/F02 Version No.: 00 Effective Date:

No. calibration parameter


The auto sampler carry
Auto sampler over should not be more
5.
carryover than 0.1%.
Calibration of Column Heater Temperature
6.
Temperature
The observed
30° C
temperature should be
between ± 2°C of the set
70° C temperature
Calibration of Sample tray chiller temperature
7.
Temperature
The observed
5° C
temperature should be
between ± 3°C of the set
15° C temperature
8. Calibration of Refractive Index Detector

Detector Linearity Correlation coefficient


NLT 0.99
9. Calibration of Photo Diode Array Detector (PDA):
The area should be
minimum at 244 ± 2 nm
9.1 Wavelength accuracy
and maximum at 205±
2nm, 272 ± 2 nm.
Detector Linearity Correlation coefficient
9.2
NLT 0.99

Calibration complies/does not complies

Calibrated By Checked By Approved By

Format No. QA001/F02

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