(N.B: 2023.

year
subjectof No 2022/ s RolNo:l Name:
Date:
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Examiners
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relain
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his/her Laboratory Callege
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tilRubber
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during of ExamNo: Class:
B.Sc.
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 s2H3E*$A3E*H3A3E*3BH3:3EHH:RE3E43E434$
IARNREE3ER&HR&HBEBEBABE3A3BBESBAR0 D

II n d e X
S. Name of the Experiment Page Date of Date of
Remarks
No. No. Experiment Submission

.To calcuate mean of tHe i3o4|osl 203

height ol students h
mñ cass' bydinect
mehod

QExamindton e 4-soofl)o23
blood

3-6 demenstrate agotose gl.6-6a os|o|2023

electo phoreis fo detecen
DNA

4To demoA'shate pols ackyl|9-lu 6la|2023

amide electn phoYesis
or defechisn of proteÉn
5. To leaun basies of 2-Is_A]42023
Csmputer applicahisns

BEB3EBBEBR
H3BBEBAHBE3A
 Dat.o4o2023
Expt. No. Page No. o1

typ:RTMET-1
Te caciulate mean
the heitt
all
dect
students
methad
class by

Rquixenent Student mesunement. Scale

nste b0ek peh

formula 01e dahect methed is

The
X <fx

Where, mear

Teacher's Signature:
 Dte 4-06 2093
Expt. No.
Page No. 0

hese
PRoCEDURE students have
heiht 0S in the table

Question Table
Hight foms) No of Students
ISS 5

9
16S
168
170
172 3
17s

Teacher's Signature
 X fx 8233
SD

165CM

Resut all the

classheght s
The meah
students L63 Cms
Expt. No. Page o 03

5
160 I120
169 9 1,4S
1,6So
1,344
1,02 o
132 51 6
350
17s. 2
<fr=8,233

Resnt R
The meaun height all the students
mu closs H65 Cms.

Teacher's Signature:
 Seum sed
Ari AniiD
Centain AAnhb Conain PAntbady Cenoin DAnlb,

Noxma Nosnmal CloHed

Thd d

8lood Gooup-5ot"
 Date o4| c62o23
Expt. No. Page No. o4

tXPERIMENT -2
Examinen Bon bood grsup.

Rguiceats: ant'sesum-A
Cavit slides blood sample
and atiseSrum-B,
Smallalastubes
ontisesam -D Qund pathalegical bisculas micaesCspe
proceduse

olth
the
sBesilzed cotton
middle
in
ngex
akohal
the tp the Fingex become dy
ick ith stestlized needle.
4 Collect the bloed in sample ef Sodium
chleoüde peodesn disselved in io cc f
disilled watex

Teacher's Signature:
 Date o4{o62023
Expt. No.
Page No. O5

B Place two dsteps blood

the too places
slides.
antisesum A 9h 4he Tst dcp and antiseum
Thd and antisem D Cn

tbsevanienR
Antiseum AB
The blood
Shemains
eh the both
hoxmal unclotted]
dhsps
'and clotted antisesuum D.

Result On he basis abave

shservaisn the blond
blood Got gkeup

Teacher's Signature
 Posiive Ene
20b

Sokb
od 120Kb

LoKb
1SoRb
2o0/Nb

Negative End
 DateaS|o2023
Expt. No. Page No. oß

tXPERIMENT-3
Aig demanctae. agârese qel elecho
phexesis fon detectien ef DNA

Rouinegent Hocase. psudex TRE

TRE bufFex
DNA Samples DNA laddest loding
Gel
qel electrephsnests appesatus ethidim
dyebomiae.

Preceduxe wfth TAE buffes

Mizes the pouwder

I9 Heatel
heat
the
agesistnt
flask. with
flask.
the mixstoe
michotD0Me until he agcthase desolve
Canpleiely
Seluten fox
kept cooltng
4 Tns ested
wells. fon
cemb
Somole
into
the gel tay fomin
Teacher's Signature :
 Date
Expt. No.
Page No. 01

I5. Aften the coling, Seluion

added ethiditum Jhomide ngatuge
IQ. Mixes the DNA sample with appepoiate qel

phces the ino. he

electrophsesß chombe ehsuing that coells
face the neoahie elechrode [ Black] and
pasHve elechsle [Red].
Using sample. pipete ntefully loading each DNA
hto sepmaed wlls intb qel
91 filed the elecko - pheAsis chambesn th
TAA buffen un the Fuly
gub menqed..
ol Connected poloest to the electaodes
fost 30 - 6o minutes

tbsevatisn ehe hsus caefully emeyed he qel

frem the dechophoresis chombe

Teacher's Signature
 Dateo5o6(2623
Expt. No. Page No. o8

Place the qel IS tansillurinato fo

the ViSualiztfen.
DNA bands appenks
shean the digan
Resutt DNA Samples have
DNA Ahagnent
lookb J6okb J2okb Aokb 4okb and gokb

Teacher's Signature :
 Poeiive End
QoKDa.

40RDa.
50RDa
120KDo
ooRDa 460RDEacl
d6oRDe
150D
200 KDo
2000a
NeyetveErd
 Date 6 olo23
Expt. No. Page No. 09

bxpcaiwent -
Aimt demengtaBe the
gel elecho phA nEs0s fobr detecHen f pateins.

Poly acsyl anie

amde qel appxators,
acjuy amide
qlass plates, space9s selutiencsmb amstiun
dis cyl amde pex salphate,
teha methyl athylene diamint Tus glycene Suuning
buffer "paotein Samples paLuer Suppt
melecula weIght makexs CAemassIe ant
blue skinin Stecqent

he acsyl amide
dis acsuy amide Solution

Added APS and TEMED to the gel Solutten

the gdl Solutien the
casling
Teacher's Signature:
 Date 6 62623
Expt. No. Page No. 1o

he gel Camb înto the qel solutisn.

the qel fox 30 minuts to polmsise.
thm
9S
psuotern
fox
Samples
5 minuts heating
C&mb
SMe Moved
Ahsqel
gel castngy
the oells.
Samgles
Ia Connected the poiaer Supply fax
abeut 4-9 hats.

thgenvotn
Aftes 1-2 huns ane Fullg Memoyed the qel
Arem he 4ppasatus.
with Coomassia bhilliant blue
Stand
and lef fox

Nou destained the gel This bymakesplacÉng in

destaining Solutien Jhe back
aund cleat and the protein becor
become yisible.

Teacher's Signature
 Date o k|2023
Expt. No. Page No. 4

The poten band visibe

the.
shon dagam.
Resut o The
have fallouaing
poobein Samples he
protein Aragnant
2ooRDa l6o kDa 120RDabKDato kpa ,20D.

Teacher's Signature
 Datt

Expt. No. Page No. 12

txpERIMINI -5
leasun basîcs of computex
applicatians
nefexs to the
Cemptlesn applicalians
hands' en Cempenent
practical leasuning computex
apploatians hexe apoly J theoxecal
Knooledgeo sttal -ps
-toold scenofeus
softoare. pDgsams Heke
sing Some Rey you might
CSmpterapplicaton practeal
Cove
CIe.

We leroun to naigate and

pestfom basictasks
ke Windoos, mac0S
Aifereht sgeIahing
Linux
Jystems
This fncludes file. manaqement inshallng Safhoate
and customizing setings

Teacher's Signature :
 L
Expt. No.
Page No. 13

Woxd Proccssing
COn lecoun how to USe word
prncessing softoane Such MicsAsofA
Waond CG1 Ghoogle Docs to Coeat. and.
Format docuntents This inclu des tasks ike
formatting text ,
yping imnag e.s editinq Cheatinq adding
Jtobles.
managing pagefaysth.

We
Speatsteat Aalysa
ean haw to
spxtadsheel sftoae. Iike MicyesofE Excel
shects. This încludes cheating
and GangleJfomatting Sprendsheets enteing
data + performing calculatisns Câneatfng
chats usihg foxmulas futtfiens

Presentaticns
We Can leag1n how to cneate and
deliyex presenaiens Softwae Like
MiBSofA Pooex point Google aldes.

Teacher's Signature
 Date

Expt. No. Page No.14

his involves CNeating slides ,adding text,

Images, and multimeota elem ents applying
tianstens and animatiens and presenhind
Alides effechvely
Dalabase Maroggrant
ale Can lean Hhe basts
wth dlatabase. using, Sodsane
MicxOs eft Access
This includes Cheating and manoqing
databases degisning thbles
J data
entexitt
and gunnita
and manipulatt
Jonetieve infoxmatibn

And Commurtation
We COn lea4n to effecively use
web besehS Senrch enqines and email
clients Ths. hcludes naviating. wehsites.
Senching. fos infoxmatfsn senting and
emajls and snline
C9mmun«catisn. managing

Teacher's Signature :
 Date

Expt. No. Page No. Ls

We leakn abeut Commeh Seouits

threats, hest prachces fox enline J
Saftty and psotec tng younpeKSOnal
îinfornhatisn ahileusing 'Compubers and
the intexnet

Rotuctiity To's
JWe Can exploke sthex productvits toals
Ike nste taking applatohs profect
management softatare and colloboatien tools.
he Specit CHUkSe
depentihg Currculum.
ds723

Teacher's Signature: