Direct and Indirect Food Production
Direct and Indirect Food Production
Yoghurt making
● Uses bacteria (eg lactobacillus)
○ Bacteria converts lactose (milk sugar) to lactic acid by lactate fermentation
○ (Link to Lac operon - glucose converted to pyruvate using enzymes made by the Lac
operon)
○ Production of lactate/lactic acid lowers the pH. This denatures the milk protein causing it to
thicken and coagulate, and gives a sour taste
Cheese making
● Uses bacteria (eg lactobacillus and rennin)
○ Bacteria converts lactose (milk sugar) to lactic acid by lactate fermentation
○ Rennin coagulates the milk protein casein in the presence of calcium ions
○ This produces curds and whey. The curds are pressed to form cheese.
○ Bacteria and fungi (if added for blue cheese) also produce fatty acids and ketones that give
flavour
Brewing
● Uses yeast
○ Respires anaerobically (alcoholic fermentation) to produce ethanol and carbon dioxide
○ Yeast cannot respire starch so the barley grains are allowed to germinate (malting) which
converts the starch in the grains to maltose which yeast can respire
○ Alcoholic fermentation will stop when the alcohol content becomes too high as ethanol is
toxic to yeast at high concentrations.
○ GM yeast allows brewing to be carried out at a lower temperature than traditional yeast
therefore reducing costs.