Antimicrobial Activity Of Tinospora Cordifolia

Of Different Solvent Extract

SAVITRIBAI PHULE PUNE UNIVERSITY

BACHELOR OF SCIENCE
In

BIOTECHNOLOGY
By

Karan Nandkumar Sagar

Under the guidance of

Mrs. Rutuja Khonde

SINHGAD COLLEGE OF SCIENCE

Ambegaon (Bk)

Pune-411041

2023-2024

Page 1
 SIGNIFICANCE

SINHGAD TECHNICAL EDUCATION SOCIETY’S

SINHGAD COLLEGE OF SCIENCE

DEPARTMENT OF BIOTECHNOLOGY
S. No.9/1/5 & 9/2/4, Off Westerly Bypass Highway, Ambegaon(Bk), PUNE0-411051

A
: (020)2434 1730, 24100171/262, Fax: 020-24356798, E-mail: [email protected],
Website: www.sinhgad.edu

CERTIFICATE
This is to certify that the project entitled “Antimicrobial activity of tinospora cordifolia of different
solvent extract” which is being submitted here for the partial fulfillment of BSc Biotechnology degree to
Savitribai Phule Pune University, by Karan Nandkumar Sagar under my supervision and guidance. To the
best of my knowledge and belief the work has been completed in the academic year 2023-2024 as prescribed
by university.

Project Guide Internal Examiner Principal

External Examiner

DATE:

PLACE:
Page 2
 ACKNOWLEDGEMENT

It gives me an immense pleasure to express my gratitude and appreciation to my guide Mrs. Rutuja
Khonde for the efforts, experience and her valuable suggestions and sincere dedication towards guiding me
for my project.

I am grateful to Dr. Magan P. Ghatule, Principal, Sinhgad College of Science, for being helpful and
supporting in granting me permission to perform the project work in the department laboratory and also
allowing me to use the college library for the literature work.

I would like express my sincere gratitude to Dr. Vibhavari Dixit, the Head of Department, Dr. Jayant
Londhe, Dr. Manisha Bachchhav, Mrs. Sonal Mahadik and Mrs. Suvarna Vaidya for providing the
guidance and facilities for the project during the course of work. Last but not the least, I would like to thank
my parents and friends whose wonderful comments and compliments has added up essence in this project.

Regards

Karan Nandkumar Sagar

Page 3
Index
 Introduction

Tinospora cordifolia (T.cordifolia) which is also known as Giloe belongs to thefamily Menispermaceae. It is
an important medicinal plant used in Ayurvedic system of medicine. The stem of the plant is greyish brown-
black in colour and bitter in taste. The stems of T.Cordifolia are rather succulent with long filiform fleshy
aeria roots from the branches. The stem is soft wooded, dry, cylindrical and 5 mm to 25 mm in diameter.
Traditionally, the plant has been in used as an anti-spasmodic, anti-inflammatory, jaundice, diabetes,
seminal weakness, urinary tract infections, fever, general debility, skin diseases, and expectorant,
carminative, digestive, anti- stress and aphrodisiac. Piles problem can be controlled by eating this plant
mixed with milk or water and thus, preventing the bleeding and constipation (Kirtikar KR et al., 1987). The
stem is bitter stomachic, diuretic (Nayampalli SS et al., 1988), stimulates bile secretion, causes constipation,
allays (satisfies) thirst, burning sensation, vomiting, enriches the blood and cures jaundice. The extract of its
stem is useful in skin diseases (Ayer KN et al., 1963; Raghunathan K et al., 1982). The root and stem of
T.Cordifolia are prescribed in combination with other drugs as an antidote in snake bite and scorpion sting
(Nadkarni KM et al., 1976; Zhao TF et al., 1991). Oral administration of an aqueous T. cordifolia root extract
to alloxan diabetic rats caused a significant reduction in blood glucose and brain lipids (Dhaliwal KS et al.,
1999). T. cordifolia is reported to benefit the immune system in a variety of ways (Kapil A et al., 1997). The
hepatoprotective action of T.Cordifolia was reported in one of the experiment in which goats treated with T.
cordifolia have shown significant clinical and hemato- biochemical improvement in CCl4 induced
hepatopathy. Extract of T. cordifolia has also exhibited in vitro inactivating property against Hepatitis B and
E surface antigen (Mehrotra R et al., 2000). The aqueous extract of T.cordifolia exerted asignificant anti-
inflammatory effect on cotton pellet granuloma and formalin induced arthritis models (Jana U et al., 1999).
In a clinical evaluation, a compound preparation ‘Rumalaya’ containing t.cordifolia reduce the pain in in
patients suffering from Rheumatoid Arthritis .
 SIGNIFICANCE

1. Tinospora cordifolia is used for diabetics , high cholesterol, allergic rhinitis, upset stomach, and
other cancer , fever .
2. It helps to boost immune system .
3. It improve platelets count in dengue and fever abd reduce the chances of complication .
4. It may act antioxidant , antiviral, antistress, antitoxic effect and antimicrobial activity
5. It has importance in traditional ayurvedic medicine used for ages in treatment of fever pain , skin
disease, cancer, snake bite, eye disorder .
 AIM AND OBJECTIVE

Aim : To use t.cordifolia solvent against various micro-organisms.

Objective:-

1. To collect plant material of tinospora cordifolia and preparation of powder.

Collection of tinospopra cordifolia plant extract

Extract preparation

Preparation of different concentration of tinospora cordifolia

Preparation Bacterial sample

Preparation of culture media

Inoculation of microbial culture and different concentration of t.cordifolia