1.

Objective: The objective of this SOP is to outline the procedures for the safe and
appropriate disposal of waste generated within the laboratory of ideSHi in accordance
with regulatory guidelines and best practices.
2. Scope: This SOP applies to all personnel working within the molecular laboratory of
ideSHi who are involved in the generation, handling, and disposal of waste materials.
3. Responsibilities:
3.1. Laboratory Personnel: Responsible for segregating waste at the point of
generation and following the disposal procedures outlined in this SOP.
3.2. Laboratory Manager: Responsible for ensuring that personnel are trained
in waste disposal procedures and that waste is properly segregated and
disposed of following regulations.
3.3. Safety Officer: Responsible for monitoring compliance with waste
disposal procedures and coordinating with waste management personnel for
the removal of hazardous waste.
4. Materials Required:
4.1. Waste bins labeled with appropriate categories (e.g., biohazardous waste,
sharps, chemical waste).
4.2. Autoclave or other appropriate sterilization equipment (e.g.: incinerators).
4.3. Personal Protective Equipment (PPE) including gloves and lab coats.
5. Procedure:
5.1. Segregation of Waste:
All waste generated within the molecular laboratory must be segregated at the point
of generation into appropriate categories:
5.1.1. Biohazardous Waste: This includes materials contaminated with
potentially infectious agents such as used culture plates, pipette tips, and
gloves.
5.1.2. Sharps Waste: This includes needles, syringes, and any other sharp objects
that may cause injury.
5.1.3. Chemical Waste: This includes expired reagents, solvents, and any other
chemical substances.
5.1.4. Non-hazardous Waste: This includes non-contaminated materials such as
paper towels and empty containers.
5.2. Disposal Procedures:
5.2.1. Biohazardous Waste:
5.2.1.1. Place biohazardous waste in designated biohazard bags or
containers.
 5.2.1.2. Seal the bags securely and label them with the biohazard
symbol.
5.2.1.3. Store biohazardous waste in a designated area until it is
collected for autoclaving or incineration.
5.2.2. Sharps Waste:
5.2.2.1. Dispose of sharps in puncture-resistant containers labeled
for sharps disposal.
5.2.2.2. When the container is full, seal it securely and label it as
"Sharps Waste."
5.2.2.3. Store sharps waste in a designated area until it is collected
for autoclaving or disposal by a licensed medical waste
management company.
5.2.3. Chemical Waste:
5.2.3.1. Collect chemical waste in appropriate containers labeled
for chemical waste disposal.
5.2.3.2. Ensure that containers are securely closed to prevent leaks
or spills.
5.2.3.3. Store chemical waste in a designated area until it is
collected for disposal by a licensed hazardous waste management
company.
5.2.4. Non-hazardous Waste:
5.2.4.1. Dispose of non-hazardous waste in regular trash bins or
designated containers.
5.2.4.2. Ensure that containers are properly closed and not
overloaded.
5.3. Autoclaving and Final Disposal:
5.3.1. Biohazardous waste and sharps waste must be autoclaved before final
disposal.
5.3.2. Load autoclave bags or containers into the autoclave chamber according to
manufacturer instructions.
5.3.3. Run the autoclave cycle at appropriate temperature and pressure settings to
ensure complete sterilization.
5.3.4. After autoclaving, allow the waste to cool before handling.
5.3.5. Dispose of autoclaved waste according to local regulations, which may
involve landfill disposal or incineration by a licensed waste management
facility.
6. Documentation:
6.1. Maintain records of waste disposal activities, including dates of disposal, types of
waste disposed, and methods of disposal.
6.2. Records should be kept in a designated logbook or electronic database for future
reference and regulatory compliance.
7. Training:
7.1. All laboratory personnel must receive training on the proper segregation and
disposal of waste according to this SOP.
7.2. Training should be provided upon initial assignment to the laboratory and
regularly as needed to ensure procedure compliance.
8. Emergency Procedures:
8.1. In the event of a spill or accident involving hazardous waste, follow the
laboratory's spill response procedures and notify the appropriate personnel for
assistance.
8.2. Contain the spill using appropriate absorbent materials and disinfect the affected
area according to established protocols.
9. References:
9.1. Refer to local, state, and national regulations governing the disposal of hazardous
waste.
9.2. Consult manufacturer instructions for proper use and maintenance of waste
disposal equipment such as autoclaves and chemical waste containers.
10. Approval: This SOP has been reviewed and approved by the Chief Operating Officer
(COO), ideSHi on 14th March, 2024.
11. Revision History:
11.1. Version 1.0: [14/03/2024] - Initial SOP creation.
11.2. If any revision is needed upon time, the version will be made according to the in-
house standard procedure.

This Standard Operating Procedure outlines the procedures for the safe and compliant disposal of
waste materials generated within the molecular laboratory of ideSHi. All personnel must adhere
to these guidelines to minimize environmental impact and ensure the safety of laboratory staff
and the surrounding community.