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Qualitative Tests For Lipids

The document describes qualitative tests to identify various lipids including fats and oils. It outlines procedures to test solubility and unsaturation of lipid samples using solvents and reagents. Practical tests are described to detect lipids using their physical properties and chemical reactions with reagents like iodine and acids.

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Jan Arby Kyle Garcia
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52 views

Qualitative Tests For Lipids

The document describes qualitative tests to identify various lipids including fats and oils. It outlines procedures to test solubility and unsaturation of lipid samples using solvents and reagents. Practical tests are described to detect lipids using their physical properties and chemical reactions with reagents like iodine and acids.

Uploaded by

Jan Arby Kyle Garcia
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
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QUALITATIVE TESTS FOR LIPIDS

NAME: TOTAL
CODE & SET: DATE SUBMITTED: SCORE:
INSTRUCTOR:

INTRODUCTION
Lipids are biological molecules that are insoluble in water but soluble in nonpolar solvents. It
constitute a very important heterogenous group of organic substances in plant and animal tissues, and
related either actually or potentially to the fatty acids.

LEARNING OBJECTIVES:
At the end of the experiment, the students must be able to:
1. Identify the physical and chemical properties of fats and oils.
2. Detect kinds of lipids using Qualitative tests.

TESTS:
I. PRACTICAL TEST FOR FATS AND OILS
II. SOLUBILITY TEST
III. TEST FOR UNSATURATED GLYCRIDES

MATERIALS:
1. Test tubes 5. Spatula 9. Vegetable oil
2. Test tube rack 6. Filter paper 10. Coconut oil
3. Droppers 7. Distilled water
4. Filter paper 8. Margarine

REAGENTS:
1. Acetic acid 4. NaOH
2. Ethanol 5. HCL
3. Hexane 6. Alcoholic Iodine

I. PRACTICAL TESTS FOR FATS AND OILS


• TEST SAMPLES:
a) Acetic acid c) Vegetable Oil
b) Margarine d) Ethyl Alcohol

PROCEDURE
1. Cut a filter paper into 4 and label them with the samples to be tested: Acetic acid, Margarine,
Vegetable Oil and Ethyl Alcohol.
2. Prepare droppers and spatula. Slowly place drops of each of the samples and let the filter paper
stand for at least 3 minutes and note which sample left a translucent spot. Find a good source
of light for better viewing of results.

TEST WITH DESCRIPTION OF RESULTS INFERENCE


Acetic acid

Margarine

Vegetable Oil

Ethyl Alcohol

✓ Expected result: presence and absence of translucent spots.

BIOCHEMISTRY 1015 LDVD, JAKMG Page 1


II. SOLUBILITY TEST
• TEST SAMPLES:
a) Hexane d) Water
b) Ethyl alcohol e) HCL
c) NaOH

PROCEDURE
1. Prepare 5 test tubes. Place 1mL of the following solvents in a separate test tube: hexane, water,
ethyl alcohol, HCL and NaOH. Make 2 sets each.
2. In the first set of the solvent, add drops of coconut oil. Shake well and observe solubility.

TEST DESCRIPTION OF RESULTS with Coconut Oil INFERENCE


Hexane

Water

Ethanol

HCl

NaOH

✓ Expected result: Miscible and immiscible

3. In another set of solvents, add a pea sized amount of margarine each of the 5 test tubes. Shake
well and observe solubility again.

TEST DESCRIPTION OF RESULTS with MARGARINE INFERENCE


Hexane

Water

Ethanol

HCl

NaOH

✓ Expected result: Soluble and insoluble.

III. TEST FOR UNSATURATED GLYCERIDES


• TEST SAMPLES:
a) Coconut Oil b) Vegetable Oil c) Alcoholic Iodine

PROCEDURE
1. Place 10 drops of the coconut oil to alcoholic iodine in a test tube. Shake well and observe for
color change.
TEST DESCRIPTION OF RESULT INFERENCE
IODINE ONLY

Coconut oil with Iodine

Vegetable oil with Iodine

✓ Note the color changes.

BIOCHEMISTRY 1015 LDVD, JAKMG Page 2

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