Igcse Biology 4ed TR Eoc Test Answers 10
Igcse Biology 4ed TR Eoc Test Answers 10
The nutrient broth remained clear in the intact flask but became cloudy in the broken
2b flask. [1] This was because the water ‘sealed’ the swan neck of Flask 1, preventing 3
microorganisms from entering the broth. [1] The nutrient broth became cloudy in Flask
2 because microorganisms were able to enter from the air and multiply. [1]
Sterilise the Petri dish and culture medium before use, and pass the inoculating loop
used to transfer bacteria to the medium through a flame. [1] Seal the lid of the Petri
3a dish using adhesive tape and store upside down. [1] After incubation, measure the 4
diameter of the zones of inhibition and calculate the cross-sectional area of the clear
area using the formula πr2. [1] The substance with the largest clear area / substance Q
is the most effective against the bacteria tested. [1]
Bacterial cells need only be counted in this study (we do not need to see their
subcellular / internal structures).
3d OR 1
The maximum magnification ability of a light microscope (× 1500) is sufficient to see
bacterial cells. [1]
[Total: 20 marks]
1
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