/EDTA Vacuum Blood Collection Tubes

TransFix® is a patented stabilisation solution that

prevents cellular degradation in whole blood for
analytical testing purposes.
Blood samples require analysing within 24-48 hours of venepuncture as the immunophenotypic profile is
detrimentally affected by natural degradation. Blood samples treated with TransFix are preserved for 10 days.
TransFix/EDTA Vacuum Blood Collection Tubes are direct draw collection tubes used to stabilise venous blood
at the point of collection and preserve whole blood specimens for immunophenotyping by flow cytometry.

TransFix Benefits TransFix /EDTA

The active components of TransFix stabilise Vacuum Blood Collection
leukocytes and leukocytic antigens for 10 days at Tube Benefits
sample storage temperatures of between 2°C and
1. Ease of application, TransFix/EDTA Vacuum Blood Collection
25°C (1) and for 4 days at temperatures between Tubes fit docking sheaths from most manufacturers.
25°C and 37°C (2). This allows:
2. TransFix/EDTA Vacuum Blood Collection Tubes are packed in
sealed foil pouches with a closed pouch stability of 18 months.
• More time in which to conduct testing –
greater flexibility in the laboratory. 3. TransFix/EDTA Vacuum Blood Collection Tubes have an open pouch
stability of 6 months.
• Time for samples to be transported to/from
remote sites.
• Reduction in the requirements of repeat The tubes are prefilled with sufficient TransFix containing
sampling. K3EDTA for the stabilisation of 3ml and 9ml of blood.

• The ability to batch samples for testing.

www.cytomark.co.uk
 A) B) Diagrams A) and B)

1E4

2
CD3-FITC are flow cytometry
analysis dot plots that

1
1E3
Fresh Blood

1E1 1E2 1E3 1E4

demonstrate the
CD8-PE
1E2
CD8-PE normal CD4+ T cell

1
(orange) and CD8+ T
1E1

1E1 1E2 1E3 1E4

cell (green) expression
CD4-APC

1
observed in a fresh
1

1 1E1 1E2 1E3 1E4

CD3-FITC 1 1E1 1E2 1E3 1E4 human blood sample.

C) D) Diagrams C) and D)
1E4
Aged Blood-10 Days

2
CD3-FITC demonstrate the
reduced CD4+ T cell

1
1E3

1E1 1E2 1E3 1E4

(orange) and CD8+ T
CD8-PE
1E2

CD8-PE cell (green) expression

1
observed in a human
1E1

1E1 1E2 1E3 1E4

blood sample after
CD4-APC 1
storage with no
1

1 1E1 1E2 1E3 1E4

CD3-FITC 1 1E1 1E2 1E3 1E4 TransFix for 10 days.

E) F) Diagrams E) and F)
1E4

demonstrate the CD4+

2
TransFix Stabilised

CD3-FITC
Blood-10 Days

T cell (orange) and

1
1E3

1E1 1E2 1E3 1E4

CD8+ T cell (green)
CD8-PE
1E2

CD8-PE expression observed in

1

a TransFix treated
1E1

1E1 1E2 1E3 1E4

human blood sample
CD4-APC
1

after it was stored for

1

1 1E1 1E2 1E3 1E4

CD3-FITC 1 1E1 1E2 1E3 1E4 10 days.

Product Information Specifications

CODE PRODUCT QUANTITY
1. TransFix/EDTA Vacuum Blood Collection
Tubes consist of purple capped tubes
TVT-03-2 Transfix/EDTA Vacuum Blood Collection Tubes (2 x 3ml tubes) 2 tubes
containing a solution of TransFix and K3EDTA
TVT-03-50 Transfix/EDTA Vacuum Blood Collection Tubes (50 x 3ml tubes) 50 tubes at the correct volume to simultaneously
TVT-09-2 Transfix/EDTA Vacuum Blood Collection Tubes (2 x 9ml tubes) 2 tubes stabilise and anti-coagulate whole blood at
the time of collection.
TVT-09-50 Transfix/EDTA Vacuum Blood Collection Tubes (50 x 9ml tubes) 50 tubes
Sample sizes are available for evaluation. 2. TransFix/EDTA Vacuum Blood Collection Tubes
are available in two sizes: 3ml and 9ml final
draw volumes.

3. TransFix/EDTA Vacuum Blood Collection

References Tubes are sterilised by gamma radiation.
1. Barbara Canonico et al, Clinical Cytometry, Vol. 78, 2010, 267-278 Flow Cytometric Profiles, Biomolecular and 4. TransFix/EDTA Vacuum Blood Collection Tubes
Morphological Aspects of Transfixed Leukocytes and Red Cells.
are CE marked and manufactured under license
2. M. Bergeron et al, Clinical Cytometry, Vol 50, 2002, 86-91 Evaluation of stabilised blood cell products as
candidate preparations for quality assessment programs for CD4 T-cell counting. by Vacutest KIMA s.r.l, 35020 Arzergrande
A wide range of additional references are available from the Cytomark website. (PD) Italy.

Cytomark, a division of Caltag Medsystems Ltd, Whiteleaf Business Centre, 11 Little Balmer, Buckingham, MK18 1TF, UK

t: +44 (0) 1280 827460 e: [email protected] www.cytomark.co.uk