Agilent PL-Latex

UNIFORM LATEX
PARTICLES
AGILENT PL-LATEX

UNIFORM LATEX PARTICLES

Unequalled Quality and Reproducibility.
Expertise in the manufacture and characterization of polymers enables us to manufacture a commercial
range of polystyrene-based uniform latex particles; all with an unequalled level of quality and batch-to-
batch reproducibility.

All of our particles are manufactured under the demanding ISO 9001:2000 Quality
Management System for maximum customer confidence. We use the highest quality raw
Applications
materials and process controls to ensure quality and reproducibility, essential for modern
sophisticated assay systems.
PL-Latex particles have been a raw
material component for many years
PL-Latex particles are used in a number of other applications where their uniformity of size,
in a variety of assays.
mobility, consistency of surface properties and versatility deliver advantages over other solid
supports. The applications in which they are used include include immunodiagnostic assays,
and assays for medical syndrome and infection disease research. Slide Agglutination
Nephelometry
Contract Manufacturing with Confidence
Particle Capture
We also undertake contract manufacturing for some of the world’s largest diagnostic and
pharmaceutical companies. Our particles, manufactured under our contract manufacturing Immunoturbidimetry
service, have been used successfully in a variety of innovative clinical tests. Membrane Migration
Particle Enhanced
Emphasis on Characterization Microfluidics
Cell Labeling
High quality characterization of particles is routine at Agilent. All of our products are
extensively characterized using state-of-the-art analytical techniques: particle size and size
uniformity (for sub-micron particles) where applicable, conductiometric titration, loading
assays and color analysis to quantify surface chemistry and color intensity.

All products are quality tested for batch-to-batch

reproducibility.

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Manufacturing Capabilities

Agilent is one of the world’s largest producers of particles for bioseparations. Agilent is ISO
9001:2000 accredited, with 40 years’ experience in bead manufacture and applications
development. Agilent’s technologies are widely used in chromatography, life science and
pharmaceutical chemistries. We manufacture superior quality, reliable particles for bead-
based assays, chromatography media, supports for peptide and oligonucleotide synthesis,
and resins for high throughput chemistry. All Agilent's beads are manufactured under
stringent quality controls to ensure batch-to-batch reproducibility of physical and chemical
properties.

Agilent manufactures polymer microparticles with engineered structure and highly controlled
surfaces with consistency between production runs. In response to client partners' needs,
Agilent applies its abilities and technologies to make bead products directed towards specific
applications. As a result, Agilent is a key OEM development partner and supplier of beads to
major bioscience companies worldwide.

• Microparticles for Life Science & Bioscience

• Synthesis & Purification of Oligonucleotides & Peptides

• Analytical and Preparative Chromatography

LodeStars are manufactured in Agilent's ISO 9001 certified, state of the art facility, where high technology
products have been developed for 40 years.

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SELECTION GUIDE

CHOOSE YOUR OPTIONS TO ACHIEVE

QUALITY CHARACTERIZATION

PL-Latex

These sub-micron uniform latex particles are used in a wide range of

applications that exploit their physico-chemical properties in sophisticated
immunoassay formats. To meet this need, we manufacture particles
with essentially uncharged surfaces for immobilization of biomolecules
by adsorption, and surfaces having functional groups, allowing covalent
immobilization of ligands. Intensely colored HiDye particles are ideal for
membrane migration assays and cell labeling studies. For many years,
PL-Latex particles have been a raw material component in a variety of assay
formats in diagnostic tests. Particle sizes available in the range are 50 nm to
1000 nm.

High Colloidal Stability

All PL-Latex products are produced by emulsion polymerization in the presence

of proprietary surfactants. The high degree of colloidal stability imparted by
the surfactants, even at low concentrations, removes the need for the high
stabilizing surface charge required for many emulsifier-free latexes.

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PL-Latex Plain

These nominally non-functionalized polystyrene particles are manufactured

using persulfate-type polymerization initiators, providing a very low surface
density of sulfate groups. They may be used in a variety of immunodiagnostic
assays where physical adsorption is used to bind ligand to the surface of the
particles. The high degree of hydrophobic bonding between the ligand and the
particle surface results in an extremely strong bond, which is stable under all PL-Latex Plain.
but the harshest of conditions.

PL-Latex Carboxyl

These carboxy-modified particles are manufactured by the co-polymerization of

styrene and a carboxylic acid, providing a moderate surface density of carboxyl
groups. They are also suitable for applications where covalent immobilization
of ligands is necessary.
PL-Latex Carboxyl.

PL-Latex SuperCarboxyl

These carboxy-modified particles are also manufactured by the co-

polymerization of styrene and a carboxylic acid, providing a high surface
density of carboxyl groups. They are suitable for applications where covalent
immobilization of ligands is necessary, and the coupling is achieved using
carbodiimide activation or other surface-modification chemistries.
PL-Latex SuperCarboxyl.

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Choosing the most suitable particle for an application can be difficult – the following guide ought to help in most cases
although this is by no means an exhaustive list. Please contact us for help with your application.

Application Size Comments

Slide Agglutination 200-1000 nm • Smaller size may increase sensitivity but handling can be more difficult
Particle Capture 200-1000 nm • Carboxy-modified particles may enhance conjugate stability
Particle Enhanced 200-1000 nm • Colored particles may improve aesthetic appearance

Immunoturbidimetry 100-200 nm
• Carboxy-modified particles may enhance conjugate stability
Nephelometry 50-100 nm

• Smaller size may improve membrane flow characteristics

Membrane Migration 200-1000 nm • White particles suitable for use with dark or colored membrane
• Carboxy-modified particles may enhance conjugate stability

HiDye Particles
PL-Latex particles are available in a range of colors (blue, red, yellow, green, purple), offering
all the features of the white particles with the added advantage of intense color. As the dye is
not surface bound, it does not interfere with the binding of ligand.
PL-Latex particles are available in a range of colors.

Magnetic Beads

In addition to our PL-Latex particles, we also offer a range of magnetic beads,

suitable for a wide variety of applications requiring a magnetically responsive
species – LodeStars beads are high performance, superparamagnetic particles
with excellent physical and chemical characteristics, designed for biomagnetic
separations. Based on proprietary technology and a wealth of experience,
LodeStars beads are a powerful magnetic platform for bioscience and life science
applications. With a unique internal architecture and a hydrophilic outer polymer
surface, they offer superior bead performance metrics compared to alternative
beads and are a fast and efficient, reliable and affordable solution to many
bioscience applications.

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Non-functionalized products:

PL-Latex Plain 15 mL 100 mL 1L PL-Latex Plain 15 mL 100 mL 1L

White HiDye
50 nm 10% Solids PL6000-7101 PL6000-7102 PL6000-7103 Blue
100 nm 10% Solids PL6001-4101 PL6001-4102 PL6001-4103 300 nm 10% Solids available on request
200 nm 10% Solids PL6002-2101 PL6002-2102 PL6002-2103 400 nm 10% Solids available on request
300 nm 10% Solids PL6003-2101 PL6003-2102 PL6003-2103 800 nm 10% Solids available on request
400 nm 10% Solids PL6004-4101 PL6004-4102 PL6004-4103 1000 nm 10% Solids available on request
600 nm 10% Solids PL6006-4101 PL6006-4102 PL6006-4103 Red
800 nm 10% Solids PL6008-4101 PL6008-4102 PL6008-4103 200 nm 10% Solids available on request
1000 nm 10% Solids PL6010-4101 PL6010-4102 PL6010-4103 300 nm 10% Solids available on request
400 nm 10% Solids available on request
800 nm 10% Solids available on request
Yellow
600 nm 10% Solids PL6006-4161 PL6006-4162 PL6006-4163
Carboxy-modified products:

PL-Latex Carboxyl 15 mL 100 mL 1L PL-Latex Carboxyl 15 mL 100 mL 1L

White HiDye
100 nm 10% Solids PL6101-6101 PL6101-6102 PL6101-6103 Blue
150 nm 10% Solids PL6115-6101 PL6115-6102 PL6115-6103 400 nm 10% Solids PL6104-6121 PL6104-6122 -
300 nm 10% Solids PL6103-6101 PL6103-6102 PL6103-6103 Red
400 nm 10% Solids PL6104-6101 PL6104-6102 PL6104-6103 400 nm 10% Solids PL6104-6141 PL6104-6142 -

PL-Latex 15 mL 100 mL 1L PL-Latex 15 mL 100 mL 1L

SuperCarboxyl SuperCarboxyl
White HiDye
50 nm 10% Solids PL6200-6101 PL6200-6102 PL6200-6103 Blue
100 nm 10% Solids PL6201-6101 PL6201-6102 PL6201-6103 200 nm 10% Solids available on request
125 nm 10% Solids PL6212-5101 PL6212-5102 PL6212-5103 300 nm 10% Solids available on request
150 nm 10% Solids PL6215-6101 PL6215-6102 PL6215-6103 400 nm 10% Solids available on request
200 nm 10% Solids PL6202-6101 PL6202-6102 PL6202-6103 800 nm 10% Solids available on request
300 nm 10% Solids PL6203-6101 PL6203-6102 PL6203-6103 Purple
400 nm 10% Solids PL6204-6101 PL6204-6102 PL6204-6103 300 nm 10% Solids available on request
600 nm 10% Solids PL6206-6101 PL6206-6102 PL6206-6103 Red
800 nm 10% Solids PL6208-6101 PL6208-6102 PL6208-6103 200 nm 10% Solids available on request
300 nm 10% Solids available on request
400 nm 10% Solids available on request
600 nm 10% Solids available on request
Green
300 nm 10% Solids available on request

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Learn more
www.agilent.com/chem/beads

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www.agilent.com/chem/contactus

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For Research Use Only. Not for use in diagnostic procedures.

This information is subject to change without notice.

© Agilent Technologies, Inc. 2016

Printed in the US, November 15, 2016
5991-1317EN